##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142553_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	513454
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.54843666618626	27.0	14.0	33.0	14.0	33.0
2	25.868395221383025	27.0	27.0	33.0	14.0	33.0
3	25.10304720578669	27.0	14.0	33.0	14.0	33.0
4	30.369012608724443	33.0	27.0	33.0	27.0	33.0
5	31.848775158047264	33.0	33.0	33.0	27.0	33.0
6	35.61081615879903	37.0	37.0	37.0	33.0	37.0
7	35.64340914668111	37.0	37.0	37.0	33.0	37.0
8	35.982387906219444	37.0	37.0	37.0	33.0	37.0
9	36.17322097013559	37.0	37.0	37.0	37.0	37.0
10-11	36.115042438076244	37.0	37.0	37.0	37.0	37.0
12-13	36.21031192667698	37.0	37.0	37.0	37.0	37.0
14-15	36.154827891106116	37.0	37.0	37.0	37.0	37.0
16-17	36.18943469132581	37.0	37.0	37.0	37.0	37.0
18-19	36.18730402333997	37.0	37.0	37.0	37.0	37.0
20-21	36.20286627429137	37.0	37.0	37.0	37.0	37.0
22-23	36.19644895161008	37.0	37.0	37.0	37.0	37.0
24-25	36.19396479528838	37.0	37.0	37.0	37.0	37.0
26-27	36.152965017314116	37.0	37.0	37.0	37.0	37.0
28-29	36.1263793835475	37.0	37.0	37.0	37.0	37.0
30-31	36.112900084525585	37.0	37.0	37.0	37.0	37.0
32-33	36.05575962014124	37.0	37.0	37.0	37.0	37.0
34-35	35.947040046430644	37.0	37.0	37.0	35.0	37.0
36-37	35.8630880273598	37.0	37.0	37.0	35.0	37.0
38-39	35.86524109267822	37.0	37.0	37.0	33.0	37.0
40-41	35.76747576219097	37.0	37.0	37.0	33.0	37.0
42-43	35.739736178898205	37.0	37.0	37.0	33.0	37.0
44-45	35.66660304525819	37.0	37.0	37.0	33.0	37.0
46-47	35.559582747432096	37.0	37.0	37.0	33.0	37.0
48-49	35.42096370853085	37.0	37.0	37.0	33.0	37.0
50-51	35.335923958134515	37.0	37.0	37.0	33.0	37.0
52-53	35.259053391345674	37.0	37.0	37.0	33.0	37.0
54-55	35.21054661177048	37.0	37.0	37.0	33.0	37.0
56-57	35.206284302001734	37.0	37.0	37.0	33.0	37.0
58-59	35.1651014501786	37.0	37.0	37.0	33.0	37.0
60-61	35.133475442785524	37.0	37.0	37.0	33.0	37.0
62-63	35.12683609437262	37.0	37.0	37.0	33.0	37.0
64-65	35.09056604875997	37.0	37.0	37.0	33.0	37.0
66-67	35.06889322120385	37.0	37.0	37.0	33.0	37.0
68-69	35.02928207784923	37.0	37.0	37.0	33.0	37.0
70-71	34.931701768805	37.0	37.0	37.0	27.0	37.0
72-73	34.94659307357621	37.0	37.0	37.0	27.0	37.0
74-75	34.87070896321774	37.0	37.0	37.0	27.0	37.0
76-77	34.7647403662256	37.0	37.0	37.0	27.0	37.0
78-79	34.76217830613842	37.0	37.0	37.0	27.0	37.0
80-81	34.77088405193065	37.0	37.0	37.0	27.0	37.0
82-83	34.69471754042232	37.0	37.0	37.0	27.0	37.0
84-85	34.62728209342998	37.0	37.0	37.0	27.0	37.0
86-87	34.50811951995699	37.0	37.0	37.0	27.0	37.0
88-89	34.42942697885302	37.0	37.0	37.0	27.0	37.0
90-91	34.380662143054685	37.0	37.0	37.0	27.0	37.0
92-93	34.21377085386422	37.0	37.0	37.0	27.0	37.0
94-95	34.26626338484071	37.0	37.0	37.0	27.0	37.0
96-97	34.274156010080745	37.0	37.0	37.0	27.0	37.0
98-99	34.24772228865682	37.0	37.0	37.0	27.0	37.0
100-101	34.131553167372346	37.0	37.0	37.0	27.0	37.0
102-103	34.07057107355284	37.0	37.0	37.0	24.5	37.0
104-105	33.907233169865265	37.0	37.0	37.0	22.0	37.0
106-107	33.88975253868895	37.0	37.0	37.0	22.0	37.0
108-109	33.77626330693694	37.0	33.0	37.0	22.0	37.0
110-111	33.70887070701562	37.0	33.0	37.0	22.0	37.0
112-113	33.579564089480264	37.0	33.0	37.0	22.0	37.0
114-115	33.561120373003234	37.0	33.0	37.0	22.0	37.0
116-117	33.41064243340202	37.0	33.0	37.0	22.0	37.0
118-119	33.29896933318272	37.0	33.0	37.0	22.0	37.0
120-121	33.20594834201311	37.0	33.0	37.0	14.0	37.0
122-123	33.164361364406545	37.0	33.0	37.0	14.0	37.0
124-125	31.752356588905727	37.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	12.0
15	232.0
16	112.0
17	22.0
18	65.0
19	257.0
20	967.0
21	2323.0
22	3825.0
23	5284.0
24	4415.0
25	3583.0
26	3593.0
27	4399.0
28	5520.0
29	7386.0
30	9851.0
31	13484.0
32	18793.0
33	28767.0
34	47692.0
35	110289.0
36	242583.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	53.554891055548524	12.810153213788267	12.00288310752238	21.632072623140832
2	19.07064702972418	19.759121557140464	35.37609990378885	25.794131509346503
3	22.259248150759365	23.864844757271342	28.657289650095237	25.218617441874052
4	15.882753919563736	17.644756061933975	34.115493232057645	32.35699678644464
5	16.503842722977264	34.71013053182663	31.53019449281121	17.255832252384902
6	29.935066452688492	33.62969570433618	18.74581262367367	17.689425219301665
7	26.52915353663619	31.676255321800976	22.486532386542905	19.308058755019925
8	26.15463118409828	31.33231019721338	21.07997990082851	21.43307871785983
9	26.66217421619074	16.075052487661992	20.922419535148233	36.34035376099904
10-11	26.0939636267319	24.657710330428824	26.053940567217314	23.194385475621964
12-13	27.1724968010704	23.815617008762242	25.976087392614318	23.03579879755304
14-15	25.162039832195287	24.201583783552177	24.87389327963167	25.76248310462086
16-17	24.0494766814554	26.12882556178353	25.81409434925037	24.007603407510704
18-19	23.798139658080373	24.838349686632103	28.051003595258777	23.312507060028747
20-21	25.26482669164805	23.677522012897096	28.013493063575932	23.04415823187892
22-23	25.351832880842295	23.477760422550023	27.778340416084013	23.392066280523668
24-25	24.231868872381945	24.31814729264939	27.969788919747433	23.48019491522123
26-27	23.84838758681401	24.579319666416076	27.99734737678546	23.574945369984455
28-29	23.57403488749159	24.39543831391731	28.404588173543505	23.625938625047592
30-31	24.02016538969411	24.14257168120221	28.331262391567698	23.506000537535982
32-33	23.834165608469732	24.444251848274035	27.88556671314943	23.836015830106806
34-35	23.93622408239102	24.468891078850298	27.91087419710432	23.684010641654364
36-37	23.98336733551142	24.479133699161064	27.707603990670904	23.82989497465661
38-39	23.874193209128762	24.517970451101753	27.77317929162106	23.834657048148422
40-41	23.808313491773664	24.47742444382769	27.92464589510997	23.789616169288674
42-43	23.94986420175072	24.331014684129084	27.80567752565754	23.913443588462652
44-45	23.954141948451078	24.655081078343922	27.292707818032387	24.098069155172617
46-47	24.089304981556285	24.59947726573364	27.157934303754573	24.153283448955506
48-49	24.125919751331182	24.822184655295313	27.130083707596008	23.9218118857775
50-51	23.90214118499418	25.044502526029593	26.910492468653473	24.142863820322756
52-53	23.98900388350271	24.96455378670728	26.813599660339584	24.232842669450427
54-55	23.946546331316924	24.8618181959825	26.902409952985078	24.289225519715497
56-57	24.116766058887457	24.804461548648955	26.55086921126333	24.527903181200266
58-59	24.01763351731606	24.851495947056602	26.757995847729298	24.37287468789804
60-61	24.015880682592794	24.74622848395377	27.202534209491013	24.03535662396242
62-63	23.809046185247364	24.87554873464809	27.051693043583263	24.263712036521284
64-65	23.72909744592505	24.680984080365526	27.044486945276503	24.545431528432925
66-67	23.957160719363372	24.72977131349644	26.978171364912924	24.334896602227268
68-69	23.816252283554125	24.512614567225107	27.17682596688311	24.494307182337657
70-71	23.719456854947087	24.654788939223376	27.218017582879867	24.40773662294967
72-73	23.92074070900217	24.636676313749625	27.331464941357943	24.111118035890264
74-75	23.877735981445028	24.715730796967275	27.275810318280158	24.130722903307543
76-77	23.866095344294507	24.70426659284703	26.981920688082155	24.4477173747763
78-79	23.81734204691193	24.77965299546306	27.16848621534848	24.234518742276535
80-81	23.420988053457563	24.903009811979263	27.330685903703156	24.345316230860018
82-83	23.84566095502226	24.719741203691083	27.45912973703584	23.97546810425082
84-85	23.83973523100043	24.754069283560504	27.112801651297826	24.293393834141245
86-87	23.5465966952145	25.061452622954018	27.17795899256716	24.213991689264326
88-89	23.716766491607093	24.629437721142004	27.14506525213848	24.50873053511242
90-91	23.98871339528945	24.64281015014693	27.04555090424417	24.322925550319447
92-93	23.82327844712835	24.93761305956868	26.908835590055148	24.330272903247828
94-95	23.458999822767208	24.832456582834904	27.154887827223344	24.55365576717454
96-97	23.60980730503609	24.771255068613744	26.961227295921347	24.657710330428824
98-99	23.4868167352869	24.917324628885936	26.74611552349383	24.849743112333332
100-101	23.752760714689146	25.152399241217328	26.523700273052697	24.571139771040833
102-103	23.763472482442438	25.034180277103697	26.698886365672486	24.503460874781382
104-105	23.67882378604093	25.192231285203448	26.606991220074633	24.521953708680986
106-107	23.786454093258598	25.08637579997429	26.46371437363425	24.66345573313286
108-109	23.718839498646897	25.018964827183925	26.42095002341998	24.841245650749197
110-111	23.580226836510025	25.38642739800196	26.39362668673989	24.639719078748122
112-113	23.54514717968893	25.379878236414555	26.185208412048596	24.889766171847917
114-115	23.55227883342893	25.367633096278436	26.34376822828163	24.73631984201101
116-117	23.70267941046153	25.409750658532193	26.06297564039517	24.824594290611106
118-119	23.48547628947899	25.246492622993124	26.293812390021685	24.974218697506203
120-121	23.501813210141513	25.144511484962628	26.53392514227175	24.81975016262411
122-123	23.442703728084695	25.34745079403413	26.456605655034338	24.753239822846837
124-125	23.571130864274473	25.202065252956483	26.312791168725948	24.914012714043096
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	167.0
1	155.5
2	252.0
3	353.5
4	278.5
5	241.5
6	343.0
7	620.0
8	1035.0
9	1332.0
10	1241.5
11	968.5
12	855.5
13	798.0
14	748.0
15	728.0
16	711.0
17	705.5
18	716.5
19	698.5
20	670.5
21	664.5
22	658.5
23	673.0
24	733.5
25	809.5
26	898.0
27	1098.5
28	1362.0
29	1778.5
30	2423.5
31	3053.0
32	3814.0
33	4672.5
34	5567.0
35	6459.5
36	7398.5
37	8675.5
38	9792.0
39	10620.0
40	11039.0
41	11343.5
42	12164.0
43	13477.0
44	15026.0
45	16458.5
46	18029.5
47	19548.0
48	20995.5
49	21981.0
50	22229.5
51	21928.5
52	21476.5
53	21663.5
54	21497.0
55	20923.5
56	20269.5
57	18566.0
58	16883.5
59	15080.5
60	13064.0
61	11100.0
62	8991.5
63	7166.0
64	5492.0
65	4245.0
66	3344.5
67	2650.0
68	2146.5
69	1811.0
70	1516.5
71	1255.0
72	1060.5
73	980.0
74	819.0
75	695.0
76	575.0
77	406.0
78	290.0
79	176.0
80	115.5
81	88.5
82	70.0
83	50.0
84	31.5
85	15.0
86	8.0
87	5.5
88	4.0
89	3.5
90	2.5
91	2.0
92	2.0
93	1.5
94	1.0
95	1.5
96	1.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02317637022985506
2	0.0
3	0.0
4	7.790376547850441E-4
5	0.0031161506191401765
6	0.001168556482177566
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	1.9475941369626103E-4
14-15	0.0
16-17	0.0
18-19	0.0
20-21	9.737970684813049E-4
22-23	0.0
24-25	0.0
26-27	0.0
28-29	6.816579479369135E-4
30-31	0.0
32-33	3.8951882739252206E-4
34-35	0.0
36-37	0.0012659361890256964
38-39	0.0
40-41	0.0022397332575070017
42-43	0.0016554550164182187
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.07478761485936422
76-77	0.09582163153856041
78-79	4.8689853424065246E-4
80-81	0.0
82-83	0.0
84-85	0.07848804371959318
86-87	0.1682721334335695
88-89	0.20586070027694786
90-91	0.08890767235234315
92-93	0.14149271405033362
94-95	9.737970684813049E-4
96-97	0.0
98-99	0.0
100-101	0.0
102-103	0.0
104-105	0.0019475941369626098
106-107	0.0
108-109	6.816579479369135E-4
110-111	9.737970684813051E-5
112-113	0.0
114-115	9.737970684813051E-5
116-117	2.921391205443915E-4
118-119	9.737970684813051E-5
120-121	0.0
122-123	0.0
124-125	0.001168556482177566
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	513454.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.76526352219673
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.31791757458546	66.15665035247922
2	8.662691506881895	13.126622096608045
3	1.9284640541255584	4.3833176176172035
4	0.7446653996332984	2.2567908095631517
5	0.3564694409650096	1.350400056616205
6	0.2209719201852896	1.0045197458306574
7	0.13762313215802643	0.7298937012292086
8	0.10216282715153589	0.6192314817047002
9	0.07412227638628836	0.5054304422945016
>10	0.4139410091680944	5.845472508251231
>50	0.029557548427574895	1.5452104439219623
>100	0.010242714400468199	1.1651715649664416
>500	5.852979657410374E-4	0.3492188800803311
>1k	5.852979657410374E-4	0.9620702988371788
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2273	0.4426881473316013	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2066	0.4023729486964753	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	864	0.1682721334335695	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	711	0.1384739431380416	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	1.94759413696261E-4	0.0
22-23	0.0	0.0	0.0	9.73797068481305E-4	0.0
24-25	0.0	0.0	0.0	0.003700428860228959	0.0
26-27	0.0	0.0	0.0	0.006524440358824744	0.0
28-29	0.0	0.0	0.0	0.012269843062864444	0.0
30-31	0.0	0.0	0.0	0.023760648470943842	0.0
32-33	0.0	3.89518827392522E-4	0.0	0.048787233130913384	0.0
34-35	0.0	3.89518827392522E-4	0.0	0.07264526130870536	0.0
36-37	0.0	3.89518827392522E-4	0.0	0.11831634382047856	0.0
38-39	0.0	3.89518827392522E-4	0.0	0.17411491584445732	0.0
40-41	0.0	3.89518827392522E-4	0.0	0.24286498887923746	0.0
42-43	0.0	3.89518827392522E-4	0.0	0.2919443611306953	0.0
44-45	0.0	3.89518827392522E-4	0.0	0.34871673022315536	0.0
46-47	0.0	3.89518827392522E-4	0.0	0.41396113381140276	0.0
48-49	0.0	3.89518827392522E-4	0.0	0.48280858655303105	0.0
50-51	0.0	3.89518827392522E-4	0.0	0.5465922945385566	0.0
52-53	0.0	3.89518827392522E-4	0.0	0.6094995851624488	0.0
54-55	0.0	3.89518827392522E-4	0.0	0.6742570902164555	0.0
56-57	0.0	3.89518827392522E-4	0.0	0.7410595691142732	0.0
58-59	0.0	3.89518827392522E-4	0.0	0.8166262216284224	0.0
60-61	0.0	3.89518827392522E-4	0.0	0.8913164567809385	0.0
62-63	0.0	3.89518827392522E-4	0.0	0.9694149816731392	0.0
64-65	0.0	3.89518827392522E-4	0.0	1.0416707241544518	0.0
66-67	0.0	3.89518827392522E-4	0.0	1.1247356140959073	0.0
68-69	0.0	3.89518827392522E-4	0.0	1.2144223241030354	0.0
70-71	0.0	3.89518827392522E-4	0.0	1.3114125121237734	0.0
72-73	0.0	3.89518827392522E-4	0.0	1.4055786886459156	0.0
74-75	0.0	3.89518827392522E-4	0.0	1.4911754509654225	0.0
76-77	0.0	3.89518827392522E-4	0.0	1.581154300093095	0.0
78-79	0.0	3.89518827392522E-4	0.0	1.6927514441410525	0.0
80-81	0.0	3.89518827392522E-4	0.0	1.8077568779286945	0.0
82-83	0.0	3.89518827392522E-4	0.0	1.9238334884916664	0.0
84-85	0.0	3.89518827392522E-4	0.0	2.037865125210827	0.0
86-87	0.0	3.89518827392522E-4	0.0	2.14293782889996	0.0
88-89	0.0	3.89518827392522E-4	0.0	2.2695314478025295	0.0
90-91	0.0	3.89518827392522E-4	0.0	2.4022599882365316	1.94759413696261E-4
92-93	0.0	3.89518827392522E-4	0.0	2.5424867660978396	3.89518827392522E-4
94-95	0.0	3.89518827392522E-4	0.0	2.6805711904084886	3.89518827392522E-4
96-97	0.0	3.89518827392522E-4	0.0	2.818363475598593	3.89518827392522E-4
98-99	0.0	3.89518827392522E-4	0.0	2.969009882092651	3.89518827392522E-4
100-101	0.0	3.89518827392522E-4	0.0	3.128615221616737	3.89518827392522E-4
102-103	0.0	3.89518827392522E-4	0.0	3.30087992303108	3.89518827392522E-4
104-105	9.73797068481305E-5	3.89518827392522E-4	0.0	3.4762607750645627	3.89518827392522E-4
106-107	1.94759413696261E-4	3.89518827392522E-4	0.0	3.649694032961083	3.89518827392522E-4
108-109	1.94759413696261E-4	3.89518827392522E-4	0.0	3.834715475972531	3.89518827392522E-4
110-111	1.94759413696261E-4	3.89518827392522E-4	0.0	4.029474889668792	3.89518827392522E-4
112-113	1.94759413696261E-4	3.89518827392522E-4	0.0	4.235238210238892	3.89518827392522E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCAACG	190	0.0	75.13095	1
GGTATCA	2015	0.0	58.44554	1
GCGCAGA	315	0.0	41.540657	1
GTATCAA	2855	0.0	41.03333	2
GCGTAAG	70	0.0027924601	33.987812	1
ATCAACG	3560	0.0	33.241432	4
CAACGCA	3705	0.0	32.10099	6
TCAACGC	3735	0.0	32.00237	5
TATCAAC	3795	0.0	31.026306	3
AACGCAG	3890	0.0	30.574337	7
ACGCAGA	4250	0.0	27.98451	8
CGCAGAG	4440	0.0	26.786976	9
CTATTCG	90	0.00954987	26.429815	9
GTCTTAT	185	9.2711525E-7	25.720507	1
GAGTACT	1950	0.0	25.46411	12-13
GTGGTAT	1030	0.0	25.408365	1
TGGTATC	1040	0.0	24.587353	2
CAGAGTA	4680	0.0	23.697887	10-11
GTACTTT	2140	0.0	22.925396	14-15
ATGGGTA	315	4.129106E-10	22.654129	5
>>END_MODULE