FastQCFastQC Report
Sun 1 Jan 2017
ERR1142552_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142552_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168368
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8580.5095980233773638No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8200.48702841395039437No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3070.18233868668630618No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2860.16986600779245462No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2780.16511451107098737No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT2510.14907820963603535No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC2340.13898127910291744No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG1820.1080965504133802No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCAACG604.3655746E-1179.351561
GCGCAGA1000.071.416411
AAAACGC401.7682053E-459.4959875
GGGTAAA551.3041059E-554.103341
GGTAAAA653.508708E-545.7661482
GTCTGTA558.497997E-443.2826731
GTTGTAA600.001303857939.675781
ACGCAAG600.001309587739.6404348
AACGCAA600.001309587739.6404347
AGTAGTG957.437786E-637.5764125
CTAGTAC1555.0677045E-934.546063
TAGTACT1251.2701094E-633.3177534
GTGTTGG902.3811453E-433.0631521
GAGTAGT1102.026309E-532.4620061
AGTAGTA1102.0304356E-532.452362
GCTGTCG750.003913748531.7123458
CTGTGCG1501.4741272E-731.7123459
GTAGTGT1152.7569506E-531.0321646
GTACTGT1551.9601794E-730.6984866
GTTCTGG1203.6717294E-529.7568381
Produced by FastQC (version 0.11.5)