ERR1142547_2.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	ERR1142547_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	159294
Sequences flagged as poor quality	0
Sequence length	125
%GC	44

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	373	0.23415822315969215	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	350	0.2197195123482366	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	281	0.17640337991386995	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	276	0.1732645297374666	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	262	0.1644757492435371	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	259	0.16259243913769508	No Hit
GTACATGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	236	0.14815372832623952	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	213	0.13371501751478398	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	201	0.12618177709141587	No Hit
GTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTAGGGCTGAA	199	0.12492623702085451	No Hit
ATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGT	198	0.12429846698557385	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	194	0.12178738684445113	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	192	0.12053184677388978	No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC	187	0.11739299659748641	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	185	0.11613745652692506	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	183	0.1148819164563637	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	181	0.11362637638580235	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	176	0.11048752620939897	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	172	0.10797644606827628	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	171	0.1073486760329956	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	170	0.1067209059977149	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	170	0.1067209059977149	No Hit
GGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTAGGGCTGA	168	0.10546536592715355	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	166	0.10420982585659222	No Hit
TATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTAGGGCTGAAA	165	0.10358205582131153	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACAAA	95	0.0	87.80327	1
TACAAGA	45	3.1414814E-4	52.960705	2
GTACAAG	60	2.1673124E-5	49.650654	1
TCTCTGC	65	3.4719465E-5	45.845818	6
TACAAAA	170	0.0	45.561775	2
CCTCAGG	70	5.3873875E-5	42.557705	2
AGGGCTA	170	3.1650416E-10	35.058563	5
CTCAGGA	85	1.6871988E-4	35.058563	3
CTAACGC	105	1.4588744E-5	34.05689	3
TATTCTC	70	0.0027590774	34.05689	3
TCTAACG	105	1.4620271E-5	34.046165	2
TCCGTCG	35	0.00278893	33.992634	32-33
AAGCTAA	90	2.3604714E-4	33.110867	9
CGCCTAC	110	2.0057123E-5	32.50885	7
GGGTGGG	55	2.0381794E-5	32.447514	20-21
ACGCCTA	130	1.7012808E-6	32.09207	6
GGGCTAT	190	1.0422809E-9	31.368189	6
TTCTCTG	95	3.2417307E-4	31.368189	5
GTCTAAC	115	2.7237511E-5	31.085629	1
GCTATTG	195	1.376975E-9	30.563875	8

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content