FastQCFastQC Report
Sun 1 Jan 2017
ERR1142546_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142546_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences853446
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31070.3640534960618481No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15510.1817338179568479No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41750.023.8331851
GTATCAA52050.022.894511
CTAACGC1400.00313387521.2746243
GTACTAG2004.8548074E-520.8540361
GTATATG3159.980795E-920.806751
ATATGCG750.00472009419.823677118-119
TCAACGC59300.019.5884384
CATGGGG37250.019.5098294
CAACGCA59200.019.4179975
ATCAACG60000.019.1613453
AACGCAG60450.019.1138746
TATCAAC61850.019.0697672
ACATGGG73650.018.84533
CATGGGT13300.018.3633614
ATGGGGG19200.018.3028875
TACATGG84500.017.5534532
GTATAAG3757.372546E-817.4776691
GTACATG84800.017.4252051
ACGCAGA66900.017.2720747
CGCAGAG68250.016.8431578
Produced by FastQC (version 0.11.5)