FastQCFastQC Report
Sun 1 Jan 2017
ERR1142546_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142546_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences853446
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42240.4949346531590751No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41200.48274876207750694No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16060.18817827958652333No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11220.13146701724537932No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9850.11541444918600592No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCAGA4750.057.608851
GTCAACG2750.051.916281
GGTATCA53150.033.689011
GTATCAA70850.029.3868581
GCGTAAG1653.4123332E-728.8423771
GCGTACA1055.911711E-428.3273331
ATCAACG81900.025.4174983
CAACGCA83250.025.362545
TCAACGC83450.025.2304824
TATCAAC82600.025.2020952
GAGTACT35400.024.86460912-13
AACGCAG86100.024.7978766
GTACTAG2403.5680205E-722.3077751
ACGCAGA95100.022.2634627
GTACTTT39650.022.12442614-15
CGCAGAG98750.021.3803338
GTAAGTA4351.8189894E-1220.5092932
GCAGAGT103800.020.4547429
ATGGGGG20700.020.4003055
GTCTAAA2701.106926E-619.8291341
Produced by FastQC (version 0.11.5)