FastQCFastQC Report
Sun 1 Jan 2017
ERR1142544_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142544_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences449866
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10360.23029079770420527No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7700.17116207937474714No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12750.051.410591
GTATCAA18550.035.6533782
GTTCTAG750.003879298231.7810921
TATCAAC23250.028.4460283
ATCAACG22750.028.2855074
CAACGCA22800.027.6884636
AACGCAG23550.026.5567347
TCAACGC24400.025.8785975
ACGCAGA25500.024.5259258
ATGGGTA2951.7826096E-1024.2317035
CGCAGAG26300.023.7798889
CATGGGG22100.023.455714
GTGGTAT6400.023.2771641
GTACATG36500.022.8562641
CATGGGT4800.022.3435864
CAGAGTA27800.021.85403410-11
ACATGGG37150.021.8123133
TACATGG37850.021.5663322
TGGGGGG8150.021.1918186
GCAGAGT27350.021.1247069
Produced by FastQC (version 0.11.5)