FastQCFastQC Report
Sun 1 Jan 2017
ERR1142544_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142544_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences449866
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29840.6633086296808384No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27340.6077365259877385No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11620.2582991379655275No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9400.20895110988605495No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18750.061.221711
GCGTACA300.00424135359.4770471
GCGCAGA3400.052.4797481
GTGGTAT8350.047.0117951
GTCAACG2000.044.6077841
GTATCAA28750.041.9959681
TGGTATC9500.041.3163032
CGTAAGA600.001310862739.6469572
GCGTAAG801.1990305E-437.1731531
TATCAAC35300.033.188882
ATCAACG35850.032.513823
CAACGCA36500.032.097745
CTAGACG750.00391749931.7175663
TCAACGC38200.030.6693084
AACGCAG38900.030.1174186
GTCTATC1454.0718733E-628.7130581
GTATATA1254.8889364E-528.5489831
ACGCAGA41650.028.271667
CGCAGAG43850.026.8532418
CAGAGTA46000.025.4688610-11
Produced by FastQC (version 0.11.5)