##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142544_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	449866
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.55911760390872	27.0	14.0	33.0	14.0	33.0
2	25.421772261073297	27.0	27.0	33.0	14.0	33.0
3	24.63549145745622	27.0	14.0	33.0	14.0	33.0
4	30.20323162897396	33.0	27.0	33.0	27.0	33.0
5	31.80325252408495	33.0	33.0	33.0	27.0	33.0
6	35.5218776257819	37.0	37.0	37.0	33.0	37.0
7	35.53058466298853	37.0	37.0	37.0	33.0	37.0
8	35.89413514246465	37.0	37.0	37.0	33.0	37.0
9	36.11236234789915	37.0	37.0	37.0	37.0	37.0
10-11	36.03527828286645	37.0	37.0	37.0	35.0	37.0
12-13	36.1349268448827	37.0	37.0	37.0	37.0	37.0
14-15	36.08478858148871	37.0	37.0	37.0	37.0	37.0
16-17	36.12533621122734	37.0	37.0	37.0	37.0	37.0
18-19	36.121251661605896	37.0	37.0	37.0	37.0	37.0
20-21	36.145663375316204	37.0	37.0	37.0	37.0	37.0
22-23	36.13221159189625	37.0	37.0	37.0	37.0	37.0
24-25	36.125741887584304	37.0	37.0	37.0	37.0	37.0
26-27	36.07446217318046	37.0	37.0	37.0	37.0	37.0
28-29	36.05070287596752	37.0	37.0	37.0	37.0	37.0
30-31	36.02171090947082	37.0	37.0	37.0	37.0	37.0
32-33	35.9560535803995	37.0	37.0	37.0	37.0	37.0
34-35	35.82817994691753	37.0	37.0	37.0	33.0	37.0
36-37	35.73138001093659	37.0	37.0	37.0	33.0	37.0
38-39	35.696954204140795	37.0	37.0	37.0	33.0	37.0
40-41	35.58546433826962	37.0	37.0	37.0	33.0	37.0
42-43	35.53016231500047	37.0	37.0	37.0	33.0	37.0
44-45	35.41637398692055	37.0	37.0	37.0	33.0	37.0
46-47	35.26072208168655	37.0	37.0	37.0	33.0	37.0
48-49	35.070417635473675	37.0	37.0	37.0	33.0	37.0
50-51	34.95330942991913	37.0	37.0	37.0	27.0	37.0
52-53	34.85051437539178	37.0	37.0	37.0	27.0	37.0
54-55	34.798536675365554	37.0	37.0	37.0	27.0	37.0
56-57	34.78194951385524	37.0	37.0	37.0	27.0	37.0
58-59	34.72975063685631	37.0	37.0	37.0	27.0	37.0
60-61	34.7084220634589	37.0	37.0	37.0	27.0	37.0
62-63	34.69909373013297	37.0	37.0	37.0	27.0	37.0
64-65	34.65581195289264	37.0	37.0	37.0	27.0	37.0
66-67	34.63050108254458	37.0	37.0	37.0	27.0	37.0
68-69	34.61026727958992	37.0	37.0	37.0	27.0	37.0
70-71	34.502699692797414	37.0	37.0	37.0	27.0	37.0
72-73	34.516987280656906	37.0	37.0	37.0	27.0	37.0
74-75	34.44920042857207	37.0	37.0	37.0	27.0	37.0
76-77	34.3304050539494	37.0	37.0	37.0	27.0	37.0
78-79	34.33349819724096	37.0	37.0	37.0	27.0	37.0
80-81	34.32320846652114	37.0	37.0	37.0	27.0	37.0
82-83	34.248255035944034	37.0	37.0	37.0	27.0	37.0
84-85	34.1949213765877	37.0	37.0	37.0	27.0	37.0
86-87	34.05869636736273	37.0	37.0	37.0	27.0	37.0
88-89	33.96854285498348	37.0	37.0	37.0	24.5	37.0
90-91	33.911241347423456	37.0	37.0	37.0	22.0	37.0
92-93	33.74970880217665	37.0	35.0	37.0	22.0	37.0
94-95	33.799010149689025	37.0	37.0	37.0	22.0	37.0
96-97	33.80706254751415	37.0	37.0	37.0	22.0	37.0
98-99	33.764276473438755	37.0	37.0	37.0	22.0	37.0
100-101	33.64406956738229	37.0	33.0	37.0	22.0	37.0
102-103	33.550866257952364	37.0	33.0	37.0	22.0	37.0
104-105	33.40380024273895	37.0	33.0	37.0	14.0	37.0
106-107	33.37830820733285	37.0	33.0	37.0	14.0	37.0
108-109	33.27153419018107	37.0	33.0	37.0	14.0	37.0
110-111	33.18899739033401	37.0	33.0	37.0	14.0	37.0
112-113	33.051114109534836	37.0	33.0	37.0	14.0	37.0
114-115	33.03277086954782	37.0	33.0	37.0	14.0	37.0
116-117	32.88140801927685	37.0	33.0	37.0	14.0	37.0
118-119	32.75863257058769	37.0	33.0	37.0	14.0	37.0
120-121	32.66175261077743	37.0	33.0	37.0	14.0	37.0
122-123	32.60632277166978	37.0	33.0	37.0	14.0	37.0
124-125	31.219118581977746	35.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	11.0
15	393.0
16	161.0
17	26.0
18	86.0
19	365.0
20	1344.0
21	3107.0
22	5079.0
23	6908.0
24	5326.0
25	3861.0
26	3698.0
27	4320.0
28	5212.0
29	6862.0
30	9043.0
31	12325.0
32	17534.0
33	26660.0
34	44972.0
35	100047.0
36	192526.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	55.68063822549122	11.834653705305428	11.97895705807932	20.505751011124033
2	19.40333343706793	19.348428198619143	35.79977148750962	25.448466876803316
3	22.6998706281426	23.980029608816846	28.644529704400867	24.675570058639686
4	15.626500453470381	18.08991161773336	34.07896608752867	32.20462184126758
5	16.184211696291225	34.818340254128024	31.628624525399463	17.368823524181288
6	29.524271046132437	33.71604754399731	18.562343144599282	18.19733826527096
7	26.020414968012695	32.321402373151116	22.40867280479076	19.249509854045428
8	26.262264763285064	31.3502242890105	20.60169028110593	21.7858206665985
9	26.735072221505963	16.013212823374072	21.060715857610933	36.190999097509035
10-11	26.04664500095584	24.64678370892666	25.797348543788594	23.509222746328906
12-13	27.33502865297666	24.011594563714528	26.088879799762598	22.564496983546213
14-15	25.359106934064812	24.104955697918935	25.377890305113077	25.158047062903176
16-17	23.594914930223666	26.166347312310776	26.373297826463883	23.865439931001674
18-19	23.215802038829338	24.445834981972407	29.248376183130087	23.089986796068164
20-21	25.067492695006372	23.144464932140526	29.197078669418612	22.590963703434486
22-23	25.344213610275055	22.874700466361094	28.949175976846437	22.831909946517406
24-25	24.087172624737143	23.728065690672334	29.237595195013625	22.947166489576894
26-27	23.477657791431223	24.036824298791196	29.34173731733449	23.143780592443083
28-29	23.060824140709663	23.84361888354775	29.846564227958545	23.248992747784044
30-31	23.620144665300334	23.542899441166927	29.714626133115196	23.122329760417546
32-33	23.506077941246755	23.915756855675433	29.073643285922763	23.504521917155056
34-35	23.657266830567323	23.88522359991642	29.289388395655596	23.168121173860662
36-37	23.5124783543288	23.911825705387688	29.1299051480575	23.44579079222601
38-39	23.505888420107322	23.991032885348083	29.155237337340452	23.347841357204146
40-41	23.341776766591124	23.82782064947378	29.291229536529983	23.539173047405118
42-43	23.567495593063605	23.843804392042838	28.92028391118106	23.6684161037125
44-45	23.69105466961273	24.032711963117904	28.529384305548767	23.746849061720603
46-47	23.686386612902506	24.06449920643036	28.14904882787319	24.10006535279394
48-49	23.796752810837006	24.29001080321696	28.142046742807857	23.77118964313818
50-51	23.675383336371276	24.51040976646379	27.873411193555413	23.940795703609517
52-53	23.566017436303255	24.632001529344294	27.493409148501996	24.30857188585045
54-55	23.595581795467986	24.526525676534792	27.416163924368593	24.461728603628636
56-57	23.7299551421979	24.55753491039554	27.043497397002664	24.6690125504039
58-59	23.449538306962516	24.69502029493227	27.522528930837183	24.33291246726803
60-61	23.74151413976606	24.58698812535288	27.685799771487506	23.985697963393545
62-63	23.557570476541905	24.49351584694109	27.622558717484765	24.326354959032244
64-65	23.40997096913303	24.3025700986516	27.762489274583984	24.524969657631384
66-67	23.75774119404445	24.252332916913037	27.671573312942076	24.318352576100438
68-69	23.426086879204032	24.21509960743866	27.873633481970188	24.485180031387124
70-71	23.44598169232616	24.250221176972698	27.966327750930276	24.337469379770866
72-73	23.597360102786162	24.459728007895684	28.219403111148655	23.7235087781695
74-75	23.606329813680485	24.3407741753212	28.226110537046452	23.826785473951862
76-77	23.56105983605828	24.16193472591828	28.054215580693683	24.22278985732976
78-79	23.263260615383246	24.42594016885028	28.177168312341898	24.133630903424574
80-81	22.976397416119468	24.66567822418231	28.282866453566175	24.075057906132045
82-83	23.384185513019432	24.439499762151396	28.550168272329984	23.62614645249919
84-85	23.472262540721683	24.43312189756098	28.076579567585487	24.01803599413185
86-87	23.08305482139239	24.91154787325239	28.028580780338135	23.97681652501709
88-89	23.471794689035498	24.371210670040405	27.981125183201993	24.175869457722104
90-91	23.531577548166926	24.347475942954965	27.91229008830835	24.20865642056976
92-93	23.617744249435432	24.579260237045577	27.776893565444638	24.026101948074352
94-95	23.01862340618498	24.49318016111536	28.018134369498338	24.470062063201322
96-97	23.109437032360745	24.440944636847416	27.690023251368185	24.75959507942365
98-99	23.01763191705975	24.902304241707533	27.393712794476578	24.686351046756144
100-101	23.277931650758227	24.993775924386373	27.272009887388688	24.456282537466713
102-103	23.39074302125522	24.92986800513931	27.355256898720953	24.32413207488452
104-105	23.366654885197363	25.122871833174393	27.108271702911356	24.402201578716888
106-107	23.356399461172884	24.96265554631824	26.895564456971634	24.78538053553725
108-109	23.300375224512297	25.187501111447013	26.692622659292585	24.8195010047481
110-111	23.002849737477383	25.674645338834228	26.61781508271352	24.70468984097487
112-113	23.00985182254271	25.5887308665247	26.445986138094458	24.955431172838132
114-115	23.091653959178956	25.525934389351495	26.639821635775984	24.742590015693562
116-117	23.256139633311403	25.50304091903331	26.47500133373642	24.765818113918872
118-119	23.014904471341403	25.34349193646983	26.54952041167906	25.09208318050971
120-121	23.018854503340993	25.310092338607497	26.884783468855172	24.786269689196338
122-123	23.000849141744432	25.615738908919543	26.900565946304013	24.482846003032012
124-125	23.070981050750287	25.442721560842884	26.829932456689253	24.65636493171757
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	182.0
1	152.5
2	254.0
3	379.0
4	311.5
5	272.0
6	361.5
7	661.0
8	1156.0
9	1553.5
10	1480.5
11	1177.0
12	1039.5
13	973.5
14	954.0
15	953.0
16	917.5
17	920.0
18	928.5
19	863.5
20	829.0
21	859.5
22	891.5
23	909.5
24	929.0
25	958.5
26	1045.0
27	1195.0
28	1326.5
29	1696.0
30	2443.5
31	3097.0
32	3820.0
33	4571.5
34	5262.0
35	6095.0
36	6797.5
37	7820.0
38	8847.0
39	9076.0
40	8766.0
41	8686.5
42	9360.5
43	10546.0
44	11784.0
45	13177.0
46	15005.0
47	16813.5
48	18036.0
49	18633.5
50	18763.0
51	18886.5
52	19145.5
53	19432.0
54	19368.0
55	18899.5
56	18156.0
57	16338.0
58	14509.5
59	13020.0
60	11437.0
61	9611.5
62	7762.0
63	6295.0
64	4755.5
65	3541.5
66	2717.0
67	2163.0
68	1795.5
69	1523.5
70	1276.5
71	1080.5
72	945.0
73	815.0
74	683.5
75	586.0
76	481.0
77	359.0
78	259.5
79	178.5
80	124.5
81	82.5
82	66.0
83	48.0
84	29.0
85	20.0
86	11.0
87	6.0
88	6.0
89	4.0
90	3.0
91	1.5
92	1.5
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02645232135791547
2	0.0
3	0.0
4	4.445768295447978E-4
5	0.0031120378068135845
6	0.00200059573295159
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	5.557210369309972E-4
22-23	0.0
24-25	0.0
26-27	0.0
28-29	7.780094517033961E-4
30-31	0.0
32-33	3.3343262215859834E-4
34-35	0.0
36-37	0.0015560189034067922
38-39	0.0
40-41	0.0021117399403377895
42-43	0.0015560189034067922
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.07613378205954661
76-77	0.09736232567031072
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.07124343693455384
86-87	0.16738317632361638
88-89	0.20183788061333818
90-91	0.0803572619402222
92-93	0.13948598026968032
94-95	4.445768295447978E-4
96-97	0.0
98-99	0.0
100-101	0.0
102-103	0.0
104-105	0.0015560189034067922
106-107	0.0
108-109	4.445768295447978E-4
110-111	0.0
112-113	0.0
114-115	0.0
116-117	4.445768295447978E-4
118-119	2.222884147723989E-4
120-121	0.0
122-123	0.0
124-125	0.0014448746960205928
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	449866.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.94269991383271
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.93529491188748	58.38675954205086
2	9.006419825477677	12.238409590008466
3	2.2106288568482158	4.505882791250921
4	0.8856059508192287	2.4068183743366136
5	0.45778297883040897	1.5551505778167454
6	0.2755928372812381	1.1234712865080532
7	0.18509314584754408	0.880300964509887
8	0.1277361317843297	0.69429901359812
9	0.10710750640231619	0.6549455849410335
>10	0.7127686978531993	9.536158136477555
>50	0.06860295205487303	3.191941340939245
>100	0.025866686836057265	2.8340832505662212
>500	3.748795193631487E-4	0.23942107086656314
>1k	0.0011246385580894463	1.7523584761297388
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2984	0.6633086296808384	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2734	0.6077365259877385	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1162	0.2582991379655275	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	940	0.20895110988605495	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	1.1114420738619945E-4	0.0
12-13	0.0	0.0	0.0	2.222884147723989E-4	2.222884147723989E-4
14-15	0.0	0.0	0.0	6.668652443171967E-4	2.222884147723989E-4
16-17	0.0	0.0	0.0	8.891536590895956E-4	2.222884147723989E-4
18-19	0.0	0.0	0.0	8.891536590895956E-4	3.3343262215859834E-4
20-21	0.0	0.0	0.0	0.001000297866475795	4.445768295447978E-4
22-23	0.0	0.0	0.0	0.0021117399403377895	4.445768295447978E-4
24-25	0.0	0.0	0.0	0.006890940857944366	4.445768295447978E-4
26-27	0.0	0.0	0.0	0.009669546042599352	4.445768295447978E-4
28-29	0.0	0.0	0.0	0.017671928974405712	4.445768295447978E-4
30-31	0.0	0.0	0.0	0.03423241587494943	4.445768295447978E-4
32-33	0.0	0.0	0.0	0.06735338967603686	4.445768295447978E-4
34-35	0.0	0.0	0.0	0.10792102537199966	4.445768295447978E-4
36-37	0.0	0.0	0.0	0.17038406992304375	4.445768295447978E-4
38-39	0.0	0.0	0.0	0.25707655168427934	4.445768295447978E-4
40-41	0.0	0.0	0.0	0.3379895346614325	4.445768295447978E-4
42-43	0.0	0.0	0.0	0.4144567493431377	4.445768295447978E-4
44-45	0.0	0.0	0.0	0.49525858811290474	4.445768295447978E-4
46-47	0.0	0.0	0.0	0.5873971360360641	4.445768295447978E-4
48-49	0.0	0.0	0.0	0.6847594617063748	6.668652443171967E-4
50-51	0.0	0.0	0.0	0.7687844824903416	6.668652443171967E-4
52-53	0.0	0.0	0.0	0.847585725527157	6.668652443171967E-4
54-55	0.0	0.0	0.0	0.9270538338082895	6.668652443171967E-4
56-57	0.0	0.0	0.0	1.0213041216717866	6.668652443171967E-4
58-59	0.0	0.0	0.0	1.127335695518221	6.668652443171967E-4
60-61	0.0	0.0	0.0	1.2266986169214833	6.668652443171967E-4
62-63	0.0	0.0	0.0	1.31950403008896	6.668652443171967E-4
64-65	0.0	0.0	0.0	1.408197107583147	6.668652443171967E-4
66-67	0.0	0.0	0.0	1.5147844024665122	6.668652443171967E-4
68-69	0.0	0.0	0.0	1.6295963686964563	6.668652443171967E-4
70-71	0.0	0.0	0.0	1.7388511245570903	6.668652443171967E-4
72-73	0.0	0.0	0.0	1.8524405045057861	6.668652443171967E-4
74-75	0.0	0.0	0.0	1.9679193359800475	6.668652443171967E-4
76-77	0.0	0.0	0.0	2.0821755811730602	6.668652443171967E-4
78-79	0.0	0.0	0.0	2.212436592229686	6.668652443171967E-4
80-81	0.0	0.0	0.0	2.3442536221897186	6.668652443171967E-4
82-83	0.0	0.0	0.0	2.4735143353798685	6.668652443171967E-4
84-85	0.0	0.0	0.0	2.600218731800136	6.668652443171967E-4
86-87	0.0	0.0	0.0	2.7329249154192583	6.668652443171967E-4
88-89	0.0	0.0	0.0	2.8820804417315378	6.668652443171967E-4
90-91	0.0	0.0	0.0	3.0317916890807486	6.668652443171967E-4
92-93	0.0	0.0	0.0	3.179835773319166	6.668652443171967E-4
94-95	0.0	0.0	0.0	3.3223226471882734	6.668652443171967E-4
96-97	0.0	0.0	0.0	3.4888166698528007	6.668652443171967E-4
98-99	0.0	0.0	0.0	3.665980536426402	6.668652443171967E-4
100-101	0.0	0.0	0.0	3.8493684786136315	6.668652443171967E-4
102-103	0.0	0.0	0.0	4.033645574459951	6.668652443171967E-4
104-105	0.0	0.0	0.0	4.220256698661379	6.668652443171967E-4
106-107	0.0	0.0	0.0	4.406200957618491	6.668652443171967E-4
108-109	0.0	0.0	0.0	4.612262318112505	6.668652443171967E-4
110-111	0.0	0.0	0.0	4.829438099345138	8.891536590895956E-4
112-113	0.0	0.0	0.0	5.053949398265262	8.891536590895956E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1875	0.0	61.22171	1
GCGTACA	30	0.004241353	59.477047	1
GCGCAGA	340	0.0	52.479748	1
GTGGTAT	835	0.0	47.011795	1
GTCAACG	200	0.0	44.607784	1
GTATCAA	2875	0.0	41.995968	1
TGGTATC	950	0.0	41.316303	2
CGTAAGA	60	0.0013108627	39.646957	2
GCGTAAG	80	1.1990305E-4	37.173153	1
TATCAAC	3530	0.0	33.18888	2
ATCAACG	3585	0.0	32.51382	3
CAACGCA	3650	0.0	32.09774	5
CTAGACG	75	0.003917499	31.717566	3
TCAACGC	3820	0.0	30.669308	4
AACGCAG	3890	0.0	30.117418	6
GTCTATC	145	4.0718733E-6	28.713058	1
GTATATA	125	4.8889364E-5	28.548983	1
ACGCAGA	4165	0.0	28.27166	7
CGCAGAG	4385	0.0	26.853241	8
CAGAGTA	4600	0.0	25.46886	10-11
>>END_MODULE