FastQCFastQC Report
Sun 1 Jan 2017
ERR1142543_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142543_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences721003
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16160.22413221581602297No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14240.19750264562005984No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25300.033.672561
GGTATCA17600.032.1567041
ATCAACG30650.026.6120783
TATCAAC31700.026.4837072
TCTAACG900.009494426.4628372
TCAACGC30400.026.4392364
CAACGCA30850.026.0535765
AACGCAG31150.025.4080286
GTACATG48750.024.3186281
CATGGGT10900.024.0333824
CATGGGG23900.023.9145414
ACATGGG47950.023.839733
TACATGG50100.023.650172
ACGCAGA34100.023.0386647
CGCAGAG35400.022.5319948
ATGGGGG12500.022.3860035
ATTAGAC1400.00314309921.2633023
TATACCC1400.00314309921.2633025
GCAGAGT37650.021.1972549
TGGGGGG7900.021.091546
Produced by FastQC (version 0.11.5)