##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142543_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	721003
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.32988212254318	33.0	33.0	33.0	27.0	33.0
2	31.406910928248564	33.0	33.0	33.0	27.0	33.0
3	31.509762095303348	33.0	33.0	33.0	27.0	33.0
4	31.49861373669735	33.0	33.0	33.0	27.0	33.0
5	31.498104723558708	33.0	33.0	33.0	27.0	33.0
6	34.89676048504653	37.0	37.0	37.0	33.0	37.0
7	34.875802181128236	37.0	37.0	37.0	27.0	37.0
8	34.88901294446764	37.0	37.0	37.0	33.0	37.0
9	34.86430847028376	37.0	37.0	37.0	27.0	37.0
10-11	34.929505841168485	37.0	37.0	37.0	33.0	37.0
12-13	34.869784869133696	37.0	37.0	37.0	30.0	37.0
14-15	34.85471905109965	37.0	37.0	37.0	27.0	37.0
16-17	34.816080515615056	37.0	37.0	37.0	27.0	37.0
18-19	34.85884940839358	37.0	37.0	37.0	27.0	37.0
20-21	34.83704020649013	37.0	37.0	37.0	27.0	37.0
22-23	34.90756002402209	37.0	37.0	37.0	27.0	37.0
24-25	34.965590295740796	37.0	37.0	37.0	27.0	37.0
26-27	35.0103577932408	37.0	37.0	37.0	30.0	37.0
28-29	35.03588889366618	37.0	37.0	37.0	33.0	37.0
30-31	34.98291615984954	37.0	37.0	37.0	27.0	37.0
32-33	34.993833590151496	37.0	37.0	37.0	27.0	37.0
34-35	35.05788048038635	37.0	37.0	37.0	33.0	37.0
36-37	35.039437422590474	37.0	37.0	37.0	33.0	37.0
38-39	35.007323825282285	37.0	37.0	37.0	27.0	37.0
40-41	34.98085444859453	37.0	37.0	37.0	27.0	37.0
42-43	34.986508377912436	37.0	37.0	37.0	27.0	37.0
44-45	34.98157774655584	37.0	37.0	37.0	27.0	37.0
46-47	34.957465502917465	37.0	37.0	37.0	27.0	37.0
48-49	34.967413450429476	37.0	37.0	37.0	27.0	37.0
50-51	34.89174455584789	37.0	37.0	37.0	27.0	37.0
52-53	34.91664181702434	37.0	37.0	37.0	27.0	37.0
54-55	34.88833472260171	37.0	37.0	37.0	27.0	37.0
56-57	34.864424281174976	37.0	37.0	37.0	27.0	37.0
58-59	34.82678019370238	37.0	37.0	37.0	27.0	37.0
60-61	34.819449433636194	37.0	37.0	37.0	27.0	37.0
62-63	34.79923592550932	37.0	37.0	37.0	27.0	37.0
64-65	34.75410712576786	37.0	37.0	37.0	27.0	37.0
66-67	34.70974461964791	37.0	37.0	37.0	27.0	37.0
68-69	34.679687185767605	37.0	37.0	37.0	27.0	37.0
70-71	34.62699461722073	37.0	37.0	37.0	27.0	37.0
72-73	34.57525419450405	37.0	37.0	37.0	27.0	37.0
74-75	34.49185232238978	37.0	37.0	37.0	27.0	37.0
76-77	34.452618088967725	37.0	37.0	37.0	27.0	37.0
78-79	34.405505941029375	37.0	37.0	37.0	27.0	37.0
80-81	34.35908519104636	37.0	37.0	37.0	27.0	37.0
82-83	34.314348900073924	37.0	37.0	37.0	27.0	37.0
84-85	34.25904746582192	37.0	37.0	37.0	27.0	37.0
86-87	34.184970104146586	37.0	37.0	37.0	27.0	37.0
88-89	34.13288849006176	37.0	37.0	37.0	27.0	37.0
90-91	34.04322866895145	37.0	37.0	37.0	27.0	37.0
92-93	33.98241893584354	37.0	37.0	37.0	27.0	37.0
94-95	33.91098025944413	37.0	37.0	37.0	24.5	37.0
96-97	33.84321077720897	37.0	37.0	37.0	22.0	37.0
98-99	33.737173076949745	37.0	33.0	37.0	22.0	37.0
100-101	33.649233775726316	37.0	33.0	37.0	22.0	37.0
102-103	33.550787583408116	37.0	33.0	37.0	22.0	37.0
104-105	33.40760579359586	37.0	33.0	37.0	22.0	37.0
106-107	33.32432458672155	37.0	33.0	37.0	22.0	37.0
108-109	33.2316391193934	37.0	33.0	37.0	22.0	37.0
110-111	33.154403657127645	37.0	33.0	37.0	14.0	37.0
112-113	33.0194728732058	37.0	33.0	37.0	14.0	37.0
114-115	32.90912936561983	37.0	33.0	37.0	14.0	37.0
116-117	32.71034309149893	37.0	33.0	37.0	14.0	37.0
118-119	32.64220883963035	37.0	33.0	37.0	14.0	37.0
120-121	32.54313782328229	37.0	33.0	37.0	14.0	37.0
122-123	32.47389608642405	37.0	33.0	37.0	14.0	37.0
124-125	30.84111577899121	35.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	1436.0
1	96.0
2	45.0
3	45.0
4	32.0
5	19.0
6	34.0
7	38.0
8	27.0
9	23.0
10	30.0
11	22.0
12	35.0
13	33.0
14	60.0
15	197.0
16	354.0
17	588.0
18	1260.0
19	2406.0
20	3763.0
21	4943.0
22	5267.0
23	5991.0
24	6522.0
25	7695.0
26	9150.0
27	10823.0
28	12843.0
29	15761.0
30	18890.0
31	22927.0
32	28937.0
33	39753.0
34	59720.0
35	115138.0
36	346100.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	44.005604035351105	19.188807527010503	11.983499848200832	24.822088589437563
2	17.60269176530858	22.370496381235043	35.64904592399967	24.377765929456704
3	19.875811844226128	26.37084403960881	28.028774014303494	25.72457010186157
4	14.78467461106147	18.245107126851444	34.0764153910819	32.893802871005185
5	15.746596634151572	36.638439275377856	31.305814991097442	16.309149099373133
6	30.810325758050045	35.355356608721664	17.922327260585572	15.911990372642723
7	27.273713762621693	32.110650777265505	21.479628604260977	19.13600685585182
8	25.819846589154405	33.37534464578343	20.530872750238576	20.273936014823594
9	26.598821638993037	17.59583747800138	19.644404872042795	36.16093601096279
10-11	25.457085061923166	26.41375693174957	25.845722140140293	22.283435866186974
12-13	26.033213695743846	25.16369567425918	25.389425224651003	23.413665405345977
14-15	24.597216053155925	25.720318746179625	23.21693871668878	26.465526483975665
16-17	24.494278335341118	27.547363886458903	23.87461401468571	24.08374376351427
18-19	24.519340491736664	26.72732662024281	25.218620754916532	23.534712133103998
20-21	25.972165598271275	25.83531729743043	24.482140148800784	23.710376955497516
22-23	25.513448232202013	25.519639803595005	24.64544557747882	24.321466386724158
24-25	24.789505350615748	26.133680187245467	24.69922131760434	24.377593144534444
26-27	24.74744277769469	26.067703351778494	24.726783273453233	24.458070597073583
28-29	24.428858189345178	26.070608332278432	25.08252569668659	24.4180077816898
30-31	24.824497035246658	25.91956041663044	24.969656923264186	24.286285624858717
32-33	24.309776534175757	26.09456800479079	24.79671488754374	24.798940573489716
34-35	24.800094024310013	26.019628270161327	24.901768241202955	24.278509464325705
36-37	24.684017041589993	26.24199002151758	24.593537493028	24.48045544386443
38-39	24.564717140029753	25.98623200852867	24.689615173581146	24.759435677860434
40-41	24.466742198046617	26.258004344097596	24.905811812801804	24.369441645053982
42-43	24.709187700588682	26.118063499900902	24.873379208667863	24.299369590842552
44-45	24.456397774687066	26.25083449235049	24.6826842837274	24.61008344923505
46-47	24.39076631003585	26.0347219566132	24.876581629282438	24.697930104068515
48-49	24.56271612132068	26.286123942122426	24.773961348659622	24.377198587897276
50-51	24.164101294588626	26.59640085309197	24.72555096682134	24.51394688549806
52-53	24.354637942184794	26.123238040930996	24.89996731639813	24.62215670048608
54-55	24.44284807261282	25.998846996104962	25.07428808268248	24.48401684859974
56-57	24.433502127192035	25.930255360398764	24.651853974073152	24.98438853833605
58-59	24.389407802925	26.00119736912554	24.89283129124648	24.716563536702978
60-61	24.446518005330226	26.11560318256058	24.9805140128757	24.457364799233495
62-63	24.008905681683068	26.340319695059666	24.91080759541727	24.73996702783999
64-65	24.292124863027794	25.863898298485378	24.955570944649324	24.888405893837504
66-67	24.507664881160967	25.90380796635907	24.850651626145144	24.73787552633482
68-69	24.552858078593513	25.792728633075917	24.728085143665332	24.926328144665238
70-71	24.234997013621918	25.777762017303612	25.065411762088225	24.92182920698625
72-73	24.52661495977374	25.901232233287264	24.95501834028104	24.61713446665795
74-75	24.564319161334378	25.692965828381176	25.143697143580322	24.599017866704123
76-77	24.478476619686965	25.70127029498802	25.124717026403914	24.695536058921103
78-79	24.52820217562422	25.70974803145226	25.140044335783763	24.62200545713976
80-81	24.31878190520629	25.79439226340688	25.24816609169785	24.638659739688983
82-83	24.44307537404711	25.90713855092861	25.089752531254323	24.56003354376996
84-85	24.42133195001415	25.828548620493752	24.946682641592616	24.80343678789948
86-87	24.13187467233666	25.906019549246494	25.131656345995797	24.830449432421045
88-89	24.233384315906562	25.52023081201335	25.19361790878754	25.052766963292548
90-91	24.75118057578313	25.548751366881866	25.037174320625823	24.662893736709183
92-93	24.42214534796416	25.930045661084634	24.91497639082774	24.73283260012347
94-95	23.948903962751277	26.03107582239289	25.21274954016069	24.807270674695143
96-97	24.25270530272699	25.87727712531696	25.066450909737974	24.80356666221807
98-99	23.974189117611484	26.014272030518175	25.130090128164667	24.881448723705667
100-101	24.151726016886247	26.270708171509117	24.7450660409618	24.83249977064284
102-103	24.29316722525651	26.126574465748547	25.141060732223014	24.439197576771928
104-105	24.212588163086853	26.335592134118414	24.951187521243597	24.50063218155114
106-107	24.10506269287442	26.305151658372488	25.121354499708083	24.46843114904501
108-109	24.25603460798767	26.226782684607326	24.93746277132191	24.579719936083094
110-111	24.135011829729738	26.533972592837664	24.719756343709946	24.611259233722656
112-113	24.15179080286573	26.495764514218834	24.845009299442033	24.507435383473407
114-115	24.074368787514672	26.527638110633056	24.907553284125715	24.490439817726557
116-117	24.076595407528185	26.729092257405828	24.78807478004744	24.406237555018546
118-119	23.940788243821455	26.393026683651026	24.96832806220556	24.697857010321957
120-121	23.800585426060078	26.32923127387154	25.207512133830733	24.66267116623765
122-123	24.01976010745896	26.528062396243108	25.062662388877193	24.389515107420742
124-125	24.039061555374264	26.4337464264917	24.92734850895931	24.599843509174725
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2368.0
1	1375.0
2	337.0
3	250.0
4	197.0
5	164.5
6	139.0
7	132.0
8	119.0
9	123.0
10	136.5
11	128.5
12	126.5
13	137.5
14	148.5
15	156.0
16	160.5
17	181.0
18	230.0
19	266.5
20	305.0
21	393.5
22	537.0
23	752.5
24	933.5
25	1142.0
26	1568.0
27	2035.5
28	2532.0
29	3306.5
30	4322.0
31	5245.5
32	6352.5
33	7602.0
34	8702.5
35	9865.5
36	11081.0
37	12489.5
38	13735.0
39	14659.5
40	15578.5
41	16398.5
42	17464.5
43	19340.0
44	21531.0
45	23179.5
46	25269.0
47	27171.0
48	29046.0
49	31033.5
50	31345.5
51	30320.5
52	30064.0
53	30544.5
54	29993.0
55	28953.0
56	27675.0
57	25810.0
58	24294.0
59	22254.5
60	19315.0
61	16179.5
62	13295.5
63	10359.5
64	7721.0
65	6298.5
66	4972.5
67	3620.0
68	2873.0
69	2510.5
70	2214.5
71	2059.0
72	1845.0
73	1818.5
74	1511.5
75	1172.5
76	915.5
77	631.0
78	453.0
79	255.0
80	163.5
81	109.5
82	68.5
83	47.0
84	26.5
85	16.0
86	9.5
87	5.0
88	2.5
89	2.5
90	2.0
91	2.5
92	3.0
93	2.0
94	0.5
95	0.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.4090135547286211
2	0.3694852864689883
3	0.35741876247394255
4	0.34798745636287226
5	0.37031746053761216
6	0.3076270140346157
7	0.3052691875068481
8	0.29805701224544146
9	0.3062400572535759
10-11	0.3063787529316799
12-13	0.3389722372861139
14-15	0.3902896381845845
16-17	0.3536046313260832
18-19	0.3338404971962669
20-21	0.32232875591363697
22-23	0.316780928789478
24-25	0.2996520125436371
26-27	0.305893318058316
28-29	0.2962539684300897
30-31	0.2968780989815576
32-33	0.2945202724537901
34-35	0.2829391833321082
36-37	0.2851583141817718
38-39	0.27967983489666476
40-41	0.29077548914498275
42-43	0.28092809599960056
44-45	0.27780744324226114
46-47	0.27815418243752105
48-49	0.2681680936140349
50-51	0.27343852938198593
52-53	0.2757270080707015
54-55	0.2789863565061449
56-57	0.2746174426458697
58-59	0.2671972238673071
60-61	0.2630363535241878
62-63	0.26511678869574745
64-65	0.2606091791573683
66-67	0.2606091791573683
68-69	0.26851483280929483
70-71	0.2641459189490196
72-73	0.25256482982733774
74-75	0.27128874637137435
76-77	0.2878628799047993
78-79	0.2697630939122306
80-81	0.27440939912871376
82-83	0.2694163547169707
84-85	0.27177418124473823
86-87	0.26296700568513587
88-89	0.2500683076214662
90-91	0.24361895858963137
92-93	0.2484039594842185
94-95	0.23994352311987607
96-97	0.23231526082415746
98-99	0.234950478708133
100-101	0.22205178064446332
102-103	0.22635134666568654
104-105	0.23141373891648162
106-107	0.2251030855627508
108-109	0.22628199882663455
110-111	0.22593525963137462
112-113	0.22260656335687923
114-115	0.212828518050549
116-117	0.22004069331195572
118-119	0.21047069152278144
120-121	0.2117883004647692
122-123	0.20422938600810261
124-125	0.20416003816905062
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	721003.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.16475357350652
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.49427831911987	71.01451538863157
2	8.999948262495964	14.609571657995863
3	1.889474010854068	4.600760774235462
4	0.6496715394703565	2.109217216193284
5	0.32353942932816604	1.312999902636676
6	0.18915526424162282	0.9211644245581695
7	0.113874102978454	0.6469794454655226
8	0.08077087828649938	0.5244598745631528
9	0.05591336948178949	0.4084375369908477
>10	0.19267685911869148	2.5038663266060475
>50	0.006994842830373723	0.3967456589202463
>100	0.003291663817147621	0.44366433644697223
>500	0.0	0.0
>1k	4.114579770474593E-4	0.50761745675624
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	1616	0.22413221581602297	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1424	0.19750264562005984	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.386956781039746E-4	0.0
2	0.0	0.0	0.0	1.386956781039746E-4	0.0
3	0.0	0.0	0.0	1.386956781039746E-4	0.0
4	0.0	0.0	0.0	1.386956781039746E-4	0.0
5	0.0	0.0	0.0	1.386956781039746E-4	0.0
6	0.0	0.0	0.0	1.386956781039746E-4	0.0
7	0.0	0.0	0.0	1.386956781039746E-4	0.0
8	0.0	0.0	0.0	1.386956781039746E-4	0.0
9	0.0	0.0	0.0	1.386956781039746E-4	0.0
10-11	0.0	0.0	0.0	1.386956781039746E-4	0.0
12-13	0.0	0.0	0.0	1.386956781039746E-4	0.0
14-15	0.0	0.0	0.0	1.386956781039746E-4	0.0
16-17	0.0	0.0	0.0	2.773913562079492E-4	0.0
18-19	0.0	0.0	0.0	4.854348733639111E-4	0.0
20-21	0.0	0.0	0.0	9.708697467278222E-4	0.0
22-23	0.0	0.0	0.0	0.002288478688715581	6.93478390519873E-5
24-25	0.0	0.0	0.0	0.0038141311478593013	1.386956781039746E-4
26-27	0.0	0.0	0.0	0.007420218778562641	1.386956781039746E-4
28-29	0.0	0.0	0.0	0.014216307005657396	1.386956781039746E-4
30-31	0.0	0.0	0.0	0.032038701642018136	1.386956781039746E-4
32-33	0.0	0.0	0.0	0.06373066408877633	1.386956781039746E-4
34-35	0.0	0.0	0.0	0.09736436602899018	1.386956781039746E-4
36-37	0.0	0.0	0.0	0.14826567989314884	1.386956781039746E-4
38-39	0.0	0.0	0.0	0.21865373653091597	1.386956781039746E-4
40-41	0.0	0.0	0.0	0.3019404912323527	1.386956781039746E-4
42-43	0.0	0.0	0.0	0.3702481126985602	1.386956781039746E-4
44-45	0.0	0.0	0.0	0.442369865312627	1.386956781039746E-4
46-47	0.0	0.0	0.0	0.5282224900589872	1.386956781039746E-4
48-49	0.0	0.0	0.0	0.6176118545969989	1.386956781039746E-4
50-51	1.386956781039746E-4	0.0	0.0	0.7083881759160503	1.386956781039746E-4
52-53	1.386956781039746E-4	0.0	0.0	0.7987484102007898	1.386956781039746E-4
54-55	1.386956781039746E-4	0.0	0.0	0.8800240775697188	1.386956781039746E-4
56-57	1.386956781039746E-4	0.0	0.0	0.9760708346567213	1.386956781039746E-4
58-59	1.386956781039746E-4	0.0	0.0	1.0783588972584024	1.386956781039746E-4
60-61	1.386956781039746E-4	0.0	0.0	1.1818952209630196	1.386956781039746E-4
62-63	1.386956781039746E-4	0.0	0.0	1.2707298027886154	1.386956781039746E-4
64-65	1.386956781039746E-4	0.0	0.0	1.360327210843783	2.080435171559619E-4
66-67	1.386956781039746E-4	0.0	0.0	1.4618524472158922	2.773913562079492E-4
68-69	1.386956781039746E-4	0.0	0.0	1.5815468174196226	2.773913562079492E-4
70-71	1.386956781039746E-4	0.0	0.0	1.7140705378479701	2.773913562079492E-4
72-73	1.386956781039746E-4	0.0	0.0	1.8373016478433515	2.773913562079492E-4
74-75	1.386956781039746E-4	0.0	0.0	1.9542914523240542	2.773913562079492E-4
76-77	1.386956781039746E-4	0.0	0.0	2.079256258295735	2.773913562079492E-4
78-79	1.386956781039746E-4	0.0	0.0	2.2155247620328904	2.773913562079492E-4
80-81	1.386956781039746E-4	0.0	0.0	2.3582426148018802	2.773913562079492E-4
82-83	1.386956781039746E-4	0.0	0.0	2.516216992162307	2.773913562079492E-4
84-85	1.386956781039746E-4	0.0	0.0	2.6621248455276882	2.773913562079492E-4
86-87	1.386956781039746E-4	0.0	0.0	2.8038024807108988	2.773913562079492E-4
88-89	1.386956781039746E-4	0.0	0.0	2.963857293242885	2.773913562079492E-4
90-91	1.386956781039746E-4	0.0	0.0	3.135285151379398	2.773913562079492E-4
92-93	1.386956781039746E-4	0.0	0.0	3.3112206190542897	2.773913562079492E-4
94-95	1.386956781039746E-4	0.0	0.0	3.4931893487267045	2.773913562079492E-4
96-97	1.386956781039746E-4	0.0	0.0	3.66857003368918	2.773913562079492E-4
98-99	1.386956781039746E-4	0.0	0.0	3.860316808667925	4.160870343119238E-4
100-101	1.386956781039746E-4	0.0	0.0	4.070856848029759	4.160870343119238E-4
102-103	1.386956781039746E-4	0.0	0.0	4.280217974127709	4.160870343119238E-4
104-105	1.386956781039746E-4	0.0	0.0	4.49873301498052	4.160870343119238E-4
106-107	1.386956781039746E-4	0.0	0.0	4.722795882957492	4.160870343119238E-4
108-109	1.386956781039746E-4	0.0	0.0	4.948592446910762	4.160870343119238E-4
110-111	1.386956781039746E-4	0.0	0.0	5.189784231133574	4.160870343119238E-4
112-113	1.386956781039746E-4	0.0	0.0	5.454554280634062	4.160870343119238E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2530	0.0	33.67256	1
GGTATCA	1760	0.0	32.156704	1
ATCAACG	3065	0.0	26.612078	3
TATCAAC	3170	0.0	26.483707	2
TCTAACG	90	0.0094944	26.462837	2
TCAACGC	3040	0.0	26.439236	4
CAACGCA	3085	0.0	26.053576	5
AACGCAG	3115	0.0	25.408028	6
GTACATG	4875	0.0	24.318628	1
CATGGGT	1090	0.0	24.033382	4
CATGGGG	2390	0.0	23.914541	4
ACATGGG	4795	0.0	23.83973	3
TACATGG	5010	0.0	23.65017	2
ACGCAGA	3410	0.0	23.038664	7
CGCAGAG	3540	0.0	22.531994	8
ATGGGGG	1250	0.0	22.386003	5
ATTAGAC	140	0.003143099	21.263302	3
TATACCC	140	0.003143099	21.263302	5
GCAGAGT	3765	0.0	21.197254	9
TGGGGGG	790	0.0	21.09154	6
>>END_MODULE