FastQCFastQC Report
Sun 1 Jan 2017
ERR1142543_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142543_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences721003
Sequences flagged as poor quality0
Sequence length125
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28080.38945746411596066No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22970.3185839726048297No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14750.20457612520336255No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21400.046.149581
GTATCAA31600.038.407541
ATCAACG40600.029.885263
CAACGCA42200.028.752175
TCAACGC42200.028.752174
CTCAACG3150.028.3304941
TATCAAC43250.028.3291822
AACGCAG43400.028.0942236
CCGCAGA4100.026.1193311
ACGCAGA48050.025.375437
ATAGACG1200.00129023424.7823053
CGCAGAG49900.024.6713338
GCAGAGT54200.022.7140129
TTAGTAC1350.00255997622.0287153
ATGGGTA5200.021.7321745
GTACATG46550.021.471531
GAGTACT23200.021.40529812-13
TACATGG48200.021.2243462
CAGAGTA54050.021.12653710-11
ACATGGG45850.020.8852393
Produced by FastQC (version 0.11.5)