##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142543_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	721003
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	22.659026384078846	14.0	14.0	33.0	14.0	33.0
2	28.014671228829837	33.0	27.0	33.0	14.0	33.0
3	31.308571531602503	33.0	33.0	33.0	27.0	33.0
4	30.97368249507977	33.0	33.0	33.0	27.0	33.0
5	32.314098554374944	33.0	33.0	33.0	33.0	33.0
6	35.13908679991623	37.0	37.0	37.0	33.0	37.0
7	35.29197243284702	37.0	37.0	37.0	33.0	37.0
8	35.58183669138686	37.0	37.0	37.0	33.0	37.0
9	35.977140178334906	37.0	37.0	37.0	33.0	37.0
10-11	36.08033323023621	37.0	37.0	37.0	37.0	37.0
12-13	36.146666518724615	37.0	37.0	37.0	37.0	37.0
14-15	36.147037529663535	37.0	37.0	37.0	37.0	37.0
16-17	36.148977882200214	37.0	37.0	37.0	37.0	37.0
18-19	36.138328828035384	37.0	37.0	37.0	37.0	37.0
20-21	36.15340712868046	37.0	37.0	37.0	37.0	37.0
22-23	36.15305622861486	37.0	37.0	37.0	37.0	37.0
24-25	36.14517137931465	37.0	37.0	37.0	37.0	37.0
26-27	36.11413059307659	37.0	37.0	37.0	37.0	37.0
28-29	36.086154981324626	37.0	37.0	37.0	37.0	37.0
30-31	36.03333966710263	37.0	37.0	37.0	37.0	37.0
32-33	36.00372813982744	37.0	37.0	37.0	37.0	37.0
34-35	35.945811598564774	37.0	37.0	37.0	35.0	37.0
36-37	35.891242477493165	37.0	37.0	37.0	33.0	37.0
38-39	35.85477314241411	37.0	37.0	37.0	33.0	37.0
40-41	35.73793798361449	37.0	37.0	37.0	33.0	37.0
42-43	35.70498597093216	37.0	37.0	37.0	33.0	37.0
44-45	35.64660757306142	37.0	37.0	37.0	33.0	37.0
46-47	35.53535768921904	37.0	37.0	37.0	33.0	37.0
48-49	35.43119099365745	37.0	37.0	37.0	33.0	37.0
50-51	35.35214000496531	37.0	37.0	37.0	33.0	37.0
52-53	35.28632266439945	37.0	37.0	37.0	33.0	37.0
54-55	35.232685578284695	37.0	37.0	37.0	33.0	37.0
56-57	35.17943961398219	37.0	37.0	37.0	33.0	37.0
58-59	35.12997310690802	37.0	37.0	37.0	33.0	37.0
60-61	35.09715216164149	37.0	37.0	37.0	33.0	37.0
62-63	35.07135476551415	37.0	37.0	37.0	33.0	37.0
64-65	35.04499703884727	37.0	37.0	37.0	33.0	37.0
66-67	35.00901452559837	37.0	37.0	37.0	30.0	37.0
68-69	34.971661005571406	37.0	37.0	37.0	27.0	37.0
70-71	34.92818892570489	37.0	37.0	37.0	27.0	37.0
72-73	34.90142690113633	37.0	37.0	37.0	27.0	37.0
74-75	34.794941213836836	37.0	37.0	37.0	27.0	37.0
76-77	34.73503161567983	37.0	37.0	37.0	27.0	37.0
78-79	34.720343743368616	37.0	37.0	37.0	27.0	37.0
80-81	34.690515850835574	37.0	37.0	37.0	27.0	37.0
82-83	34.640633256727085	37.0	37.0	37.0	27.0	37.0
84-85	34.56968209563622	37.0	37.0	37.0	27.0	37.0
86-87	34.39640056976185	37.0	37.0	37.0	27.0	37.0
88-89	34.32350489526396	37.0	37.0	37.0	27.0	37.0
90-91	34.278838645608964	37.0	37.0	37.0	27.0	37.0
92-93	34.21928549534468	37.0	37.0	37.0	27.0	37.0
94-95	34.22919252763165	37.0	37.0	37.0	27.0	37.0
96-97	34.19237784031412	37.0	37.0	37.0	27.0	37.0
98-99	34.12512777339346	37.0	37.0	37.0	27.0	37.0
100-101	34.07476113136838	37.0	37.0	37.0	27.0	37.0
102-103	33.979034761297804	37.0	37.0	37.0	24.5	37.0
104-105	33.87268915663319	37.0	35.0	37.0	22.0	37.0
106-107	33.80340095672278	37.0	33.0	37.0	22.0	37.0
108-109	33.71002825230963	37.0	33.0	37.0	22.0	37.0
110-111	33.62530252994786	37.0	33.0	37.0	22.0	37.0
112-113	33.557696015134475	37.0	33.0	37.0	22.0	37.0
114-115	33.44532477673464	37.0	33.0	37.0	22.0	37.0
116-117	33.313056256353995	37.0	33.0	37.0	22.0	37.0
118-119	33.16775727701549	37.0	33.0	37.0	14.0	37.0
120-121	33.085179950707555	37.0	33.0	37.0	14.0	37.0
122-123	32.947281772752675	37.0	33.0	37.0	14.0	37.0
124-125	31.635265040506074	37.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	13.0
16	16.0
17	14.0
18	58.0
19	372.0
20	1304.0
21	3180.0
22	4995.0
23	6879.0
24	6322.0
25	4938.0
26	5219.0
27	6459.0
28	8192.0
29	10788.0
30	14667.0
31	19753.0
32	28372.0
33	42500.0
34	70538.0
35	147992.0
36	338432.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	44.08012297583769	13.661361391358396	12.304381846635367	29.954133786168548
2	17.808386372872235	20.287155531946468	37.30275740877638	24.60170068640491
3	20.347072064887385	25.359395175886924	28.264792240808983	26.028740518416704
4	14.793142330891826	17.768858104612605	33.855060242467786	33.58293932202779
5	16.842582678213784	34.57366835509051	31.998868219720716	16.584880746974985
6	31.228233205500732	34.05377291104654	18.33910082594193	16.3788930575108
7	27.379722166353414	30.74576764895587	22.361100515303043	19.51340966938767
8	26.386714063603062	31.294183241956	21.281048761239553	21.038053933201386
9	26.869236327726792	15.628506400112066	20.66135647147099	36.84090080069015
10-11	25.83595352585218	24.545736980289956	26.77741978882196	22.840889705035902
12-13	26.793855226677284	23.28194196140654	26.67880716169003	23.245395650226143
14-15	24.873336171971545	24.001980574283323	24.95058966467546	26.17409358906967
16-17	24.21605735343681	25.966188767591813	25.698922195885455	24.118831683085922
18-19	24.062729281292867	24.792476591636927	27.815973026464526	23.328821100605683
20-21	25.312568697845983	23.95958954315629	27.484963623515345	23.242878135482382
22-23	24.80885988606142	23.778211587338465	27.65246999837033	23.76045852822979
24-25	24.13082885924192	24.405793041083047	27.59981581213948	23.863562287535558
26-27	24.055378410353356	24.44532130934268	27.65043973464743	23.848860545656535
28-29	23.747543352801586	24.46099392096843	28.02755328341214	23.76390944281785
30-31	24.17763865060201	24.291646498003473	27.87152064554516	23.659194205849353
32-33	23.792619448185377	24.341576942120906	27.67207626043165	24.19372734926207
34-35	24.21522300246463	24.34323946846121	27.80675754957344	23.634779979500724
36-37	24.063090108814073	24.49013558243025	27.45539011415387	23.991384194601807
38-39	24.01293753285354	24.242478880115616	27.4713836142152	24.273199972815647
40-41	23.82096979893465	24.614260084648144	27.70676762121793	23.858002495199273
42-43	24.2065019372386	24.42675143775906	27.505806167956887	23.860940457045455
44-45	23.93727904044782	24.564183505477786	27.24953987708789	24.248997576986504
46-47	23.98032922404323	24.463707127084696	27.214314643645764	24.34164900522631
48-49	24.129441219510504	24.857027928621463	26.951570508152496	24.06196034371554
50-51	23.901218164140786	25.13554035142711	26.767919134871835	24.195322349560268
52-53	24.169871692628185	24.722157882838214	26.76833522190615	24.339635202627452
54-55	24.1179995090173	24.746984409218825	26.86015176081098	24.274864320952894
56-57	24.104754071758368	24.708635054223073	26.504952129186705	24.681658744831854
58-59	24.06676814574152	24.723978072198086	26.686870017787733	24.52238376427266
60-61	24.03464340647681	24.784085503111637	26.961330257987832	24.21994083242372
62-63	23.649832247577333	25.010159458421118	26.85966632593762	24.480341968063936
64-65	23.810511190660787	24.57625002947283	27.00293896141902	24.610299818447356
66-67	24.011973182401697	24.63851239589178	26.909214232163503	24.440300189543024
68-69	24.050177322424457	24.60613894810424	26.858418064834684	24.485265664636625
70-71	23.715504651159566	24.565986549293136	27.27020553312538	24.448303266421913
72-73	23.979650570108586	24.755028758548857	27.125199201667677	24.140121469674884
74-75	24.021189063744856	24.646409407244754	27.174495396035343	24.157906132975047
76-77	23.883587422169033	24.55986263593319	27.23402188806216	24.322528053835615
78-79	24.068759960111013	24.612379178392263	27.122084435389134	24.19677642610759
80-81	23.760788790060513	24.751838757952463	27.364379898557985	24.122992553429043
82-83	23.909262513470818	24.73914810340595	27.167085296455078	24.184504086668156
84-85	23.73798531486438	24.835241549732174	26.965148305158255	24.461624830245192
86-87	23.67900762695669	24.848937611841762	27.062249170416443	24.40980559078511
88-89	23.694571419038404	24.63186635970327	27.01111074038237	24.66245148087596
90-91	24.110946439410462	24.521460488283232	26.918845865899204	24.448747206407102
92-93	23.92652433267054	24.945361185474823	26.692900703153636	24.435213778700998
94-95	23.495875872571606	24.899410680429014	27.00252842922548	24.602185017773902
96-97	23.773271401089872	24.783946807433534	26.723744561395723	24.719037230080872
98-99	23.54442353221831	24.856623342760017	26.68428564097514	24.91466748404653
100-101	23.775021584529874	25.111424717667415	26.396718457980384	24.71683523982233
102-103	23.942618824054822	24.951005751709772	26.62360628180465	24.48276914243075
104-105	23.769958612578225	24.99812757977897	26.441416825706803	24.790496981936
106-107	23.899440418704675	25.073458481481246	26.48590020187646	24.541200897937614
108-109	23.928095391985664	25.08089649825308	26.26545979586204	24.725548313899218
110-111	23.753949386822153	25.287379437945734	26.158186291778897	24.800484883453215
112-113	23.850247502437576	25.266885158591574	26.21951642364872	24.663350915322127
114-115	23.68318855816134	25.31424973266408	26.35779601471839	24.644765694456193
116-117	23.767792921804766	25.55197412493429	26.084218789658298	24.596014163602646
118-119	23.49379960971036	25.242821458440535	26.342262098770743	24.921116833078365
120-121	23.466557928877997	25.204282105213522	26.483546127915307	24.845613837993177
122-123	23.57715571225085	25.4073838805109	26.326312095788783	24.689148311449465
124-125	23.46786351790492	25.267647984821146	26.4019705881945	24.862517909079436
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	154.0
1	143.5
2	346.5
3	501.5
4	364.5
5	332.0
6	444.0
7	888.0
8	1490.0
9	1715.5
10	1460.0
11	1106.5
12	979.0
13	981.0
14	975.5
15	934.0
16	937.0
17	929.5
18	917.0
19	912.5
20	911.0
21	939.5
22	969.5
23	1021.0
24	1164.0
25	1368.5
26	1636.0
27	1987.0
28	2365.5
29	3003.0
30	3936.0
31	4844.5
32	5858.5
33	6929.5
34	7986.0
35	9131.0
36	10457.5
37	11908.5
38	13113.5
39	14122.5
40	15002.0
41	15788.5
42	16888.0
43	18497.5
44	20567.5
45	22434.5
46	24651.0
47	26763.0
48	28783.5
49	30798.0
50	31145.5
51	30259.0
52	29782.5
53	30132.5
54	29992.5
55	29133.0
56	27745.0
57	25866.0
58	24477.0
59	22299.0
60	19401.5
61	16296.0
62	13205.0
63	10304.0
64	7783.5
65	6311.5
66	4899.5
67	3658.0
68	2922.5
69	2427.0
70	2079.0
71	1886.5
72	1756.0
73	1814.0
74	1446.5
75	1152.5
76	949.5
77	595.5
78	391.0
79	236.5
80	149.0
81	98.5
82	72.0
83	41.0
84	19.5
85	7.5
86	3.5
87	2.0
88	0.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.029264788079938642
2	0.0
3	0.0
4	0.0
5	0.002080435171559619
6	0.0011095654248317968
7	0.009431306111070273
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	2.0804351715596192E-4
22-23	6.93478390519873E-5
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	2.773913562079492E-4
36-37	3.467391952599365E-4
38-39	0.0
40-41	0.0025658700449235298
42-43	3.467391952599365E-4
44-45	0.0
46-47	0.004854348733639111
48-49	0.008044349330030526
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	6.93478390519873E-5
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.008113697169082513
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.07447957914183437
76-77	0.04029109448920462
78-79	2.773913562079492E-4
80-81	0.0
82-83	0.0
84-85	0.06636588197275185
86-87	0.2101933001665735
88-89	0.23564395709865285
90-91	0.14590785336538128
92-93	0.1135917603671552
94-95	2.773913562079492E-4
96-97	0.0
98-99	0.0
100-101	6.93478390519873E-5
102-103	0.0
104-105	0.0015256524591437206
106-107	0.0024271743668195555
108-109	0.002773913562079492
110-111	6.93478390519873E-4
112-113	0.0
114-115	0.0
116-117	0.0
118-119	0.0
120-121	3.467391952599365E-4
122-123	0.0
124-125	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	721003.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.04765722460844
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.88990743634307	68.68443619306002
2	9.400941259510901	14.862447645409931
3	1.985401508945689	4.708240137970775
4	0.6758685569961324	2.13703304089284
5	0.35488202702146415	1.4026296413583461
6	0.1884015500650898	0.8935620690078074
7	0.12921355123257192	0.7149819954624709
8	0.07739784243105392	0.48944944947313646
9	0.06046827131825151	0.43018876657167904
>10	0.21927403685321678	2.8017056593048073
>50	0.01069476320553048	0.588958397037507
>100	0.006290996731028772	0.9077173723692636
>500	6.290996729990086E-4	0.2879300722526521
>1k	6.290996729990086E-4	1.0907195598286996
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2808	0.38945746411596066	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2297	0.3185839726048297	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1475	0.20457612520336255	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.386956781039746E-4	0.0
2	0.0	0.0	0.0	1.386956781039746E-4	0.0
3	0.0	0.0	0.0	1.386956781039746E-4	0.0
4	0.0	0.0	0.0	1.386956781039746E-4	1.386956781039746E-4
5	0.0	0.0	0.0	1.386956781039746E-4	1.386956781039746E-4
6	0.0	0.0	0.0	1.386956781039746E-4	1.386956781039746E-4
7	0.0	0.0	0.0	1.386956781039746E-4	1.386956781039746E-4
8	0.0	0.0	0.0	1.386956781039746E-4	1.386956781039746E-4
9	0.0	0.0	0.0	1.386956781039746E-4	1.386956781039746E-4
10-11	0.0	0.0	0.0	2.080435171559619E-4	1.386956781039746E-4
12-13	0.0	2.773913562079492E-4	0.0	2.773913562079492E-4	1.386956781039746E-4
14-15	0.0	2.773913562079492E-4	0.0	2.773913562079492E-4	1.386956781039746E-4
16-17	0.0	2.773913562079492E-4	0.0	4.160870343119238E-4	1.386956781039746E-4
18-19	0.0	2.773913562079492E-4	0.0	6.241305514678858E-4	1.386956781039746E-4
20-21	0.0	2.773913562079492E-4	0.0	0.0011095654248317968	1.386956781039746E-4
22-23	0.0	2.773913562079492E-4	0.0	0.002496522205871543	1.386956781039746E-4
24-25	0.0	2.773913562079492E-4	0.0	0.004091522504067251	1.386956781039746E-4
26-27	0.0	2.773913562079492E-4	0.0	0.007558914456666616	1.386956781039746E-4
28-29	0.0	2.773913562079492E-4	0.0	0.014493698361865346	1.386956781039746E-4
30-31	0.0	2.773913562079492E-4	0.0	0.0322467451591741	1.386956781039746E-4
32-33	0.0	2.773913562079492E-4	0.0	0.06373066408877633	1.386956781039746E-4
34-35	0.0	2.773913562079492E-4	0.0	0.09750306170709415	1.386956781039746E-4
36-37	0.0	2.773913562079492E-4	0.0	0.14868176692746077	1.386956781039746E-4
38-39	0.0	2.773913562079492E-4	0.0	0.21913917140427988	1.386956781039746E-4
40-41	0.0	2.773913562079492E-4	0.0	0.30228723042761263	1.386956781039746E-4
42-43	0.0	2.773913562079492E-4	0.0	0.3708028954109761	1.386956781039746E-4
44-45	0.0	2.773913562079492E-4	0.0	0.4429939958640949	1.386956781039746E-4
46-47	0.0	2.773913562079492E-4	0.0	0.529193359805715	5.547827124158984E-4
48-49	0.0	2.773913562079492E-4	0.0	0.6191375070561427	5.547827124158984E-4
50-51	0.0	2.773913562079492E-4	0.0	0.7106073067657139	5.547827124158984E-4
52-53	0.0	2.773913562079492E-4	0.0	0.8020077586362331	6.241305514678858E-4
54-55	0.0	3.4673919525993646E-4	0.0	0.8836995130394742	6.93478390519873E-4
56-57	0.0	4.160870343119238E-4	0.0	0.9799543136436326	6.93478390519873E-4
58-59	0.0	4.160870343119238E-4	0.0	1.082242376245314	7.628262295718603E-4
60-61	0.0	4.160870343119238E-4	0.0	1.185778699949931	8.321740686238476E-4
62-63	0.0	4.160870343119238E-4	0.0	1.2751680644879424	8.321740686238476E-4
64-65	0.0	4.160870343119238E-4	0.0	1.3642800376697461	8.321740686238476E-4
66-67	0.0	4.160870343119238E-4	0.0	1.4662907089152195	8.321740686238476E-4
68-69	0.0	4.160870343119238E-4	0.0	1.5861931226361055	8.321740686238476E-4
70-71	0.0	4.160870343119238E-4	0.0	1.7188555387425573	8.321740686238476E-4
72-73	0.0	4.160870343119238E-4	0.0	1.8431268663237184	8.321740686238476E-4
74-75	0.0	4.160870343119238E-4	0.0	1.960602105677785	8.321740686238476E-4
76-77	0.0	4.160870343119238E-4	0.0	2.085636259488518	8.321740686238476E-4
78-79	0.0	4.160870343119238E-4	0.0	2.221835415386621	8.321740686238476E-4
80-81	0.0	4.160870343119238E-4	0.0	2.363513050569831	9.015219076758348E-4
82-83	0.0	4.160870343119238E-4	0.0	2.5217648192864663	0.0011095654248317968
84-85	0.0	4.160870343119238E-4	0.0	2.666909846422276	0.0011095654248317968
86-87	0.0	4.160870343119238E-4	0.0	2.807963351054018	0.0011095654248317968
88-89	0.0	4.160870343119238E-4	0.0	2.9680875114250567	0.0012482611029357713
90-91	0.0	4.160870343119238E-4	0.0	3.1412490655378686	0.0012482611029357713
92-93	0.0	4.854348733639111E-4	0.0	3.318294098637592	0.0012482611029357713
94-95	0.0	5.547827124158984E-4	0.0	3.499846741275695	0.0012482611029357713
96-97	0.0	5.547827124158984E-4	0.0	3.6770304700535226	0.0012482611029357713
98-99	0.0	5.547827124158984E-4	0.0	3.8696094191008914	0.0012482611029357713
100-101	0.0	5.547827124158984E-4	0.0	4.080288154140829	0.0012482611029357713
102-103	0.0	5.547827124158984E-4	0.0	4.291660367571286	0.0012482611029357713
104-105	0.0	5.547827124158984E-4	0.0	4.5114236695270336	0.0012482611029357713
106-107	0.0	5.547827124158984E-4	0.0	4.737844364031773	0.0012482611029357713
108-109	0.0	5.547827124158984E-4	0.0	4.966068102351863	0.0012482611029357713
110-111	0.0	5.547827124158984E-4	0.0	5.209132278229077	0.0012482611029357713
112-113	0.0	5.547827124158984E-4	0.0	5.476121458579229	0.0012482611029357713
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2140	0.0	46.14958	1
GTATCAA	3160	0.0	38.40754	1
ATCAACG	4060	0.0	29.88526	3
CAACGCA	4220	0.0	28.75217	5
TCAACGC	4220	0.0	28.75217	4
CTCAACG	315	0.0	28.330494	1
TATCAAC	4325	0.0	28.329182	2
AACGCAG	4340	0.0	28.094223	6
CCGCAGA	410	0.0	26.119331	1
ACGCAGA	4805	0.0	25.37543	7
ATAGACG	120	0.001290234	24.782305	3
CGCAGAG	4990	0.0	24.671333	8
GCAGAGT	5420	0.0	22.714012	9
TTAGTAC	135	0.002559976	22.028715	3
ATGGGTA	520	0.0	21.732174	5
GTACATG	4655	0.0	21.47153	1
GAGTACT	2320	0.0	21.405298	12-13
TACATGG	4820	0.0	21.224346	2
CAGAGTA	5405	0.0	21.126537	10-11
ACATGGG	4585	0.0	20.885239	3
>>END_MODULE