FastQCFastQC Report
Sun 1 Jan 2017
ERR1142542_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142542_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences285787
Sequences flagged as poor quality0
Sequence length125
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15420.5395626812976098No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14700.5143690930658147No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8110.2837777785553577No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4320.15116152939077004No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3980.13926455717020017No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3670.12841731779262178No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAACG2250.039.6802061
GTATCAA23550.032.3507421
GGTATCA17150.030.1939771
ATCAACG28100.027.1029643
TATCAAC28800.026.6508062
CCGTTAT450.00954170426.43495622-23
CAACGCA29900.026.2673245
CCGCAGA2308.192728E-925.8783971
AACGCAG31100.025.6319436
TCAACGC30250.025.570024
ATGGGTG4150.024.373285
ACGCAGA32900.024.0487697
CATGGGT6700.023.97744
TGTTATC1250.001630617223.7997912
GAGTACT13550.023.4841912-13
CGCAGAG34050.023.2324858
GTTATCA1300.002048515522.8844133
CATGGGG9700.022.6956754
GATTAAA1852.6742879E-522.52121
ATGGGAG3204.965841E-1022.3123045
Produced by FastQC (version 0.11.5)