##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142542_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	285787
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	21.836224180945948	14.0	14.0	33.0	14.0	33.0
2	27.541494189728713	27.0	27.0	33.0	14.0	33.0
3	31.18958175144426	33.0	33.0	33.0	27.0	33.0
4	30.837000983249762	33.0	33.0	33.0	27.0	33.0
5	32.30865994604373	33.0	33.0	33.0	33.0	33.0
6	34.988232494830065	37.0	37.0	37.0	33.0	37.0
7	35.13732255141067	37.0	37.0	37.0	33.0	37.0
8	35.48136199337269	37.0	37.0	37.0	33.0	37.0
9	35.92332401403843	37.0	37.0	37.0	33.0	37.0
10-11	36.04433896573322	37.0	37.0	37.0	33.0	37.0
12-13	36.11157085521735	37.0	37.0	37.0	37.0	37.0
14-15	36.11455209649144	37.0	37.0	37.0	37.0	37.0
16-17	36.12048833571856	37.0	37.0	37.0	37.0	37.0
18-19	36.11866180057176	37.0	37.0	37.0	37.0	37.0
20-21	36.13855773705592	37.0	37.0	37.0	37.0	37.0
22-23	36.13505687802454	37.0	37.0	37.0	37.0	37.0
24-25	36.12155731366367	37.0	37.0	37.0	37.0	37.0
26-27	36.07838355138617	37.0	37.0	37.0	37.0	37.0
28-29	36.03945245934909	37.0	37.0	37.0	37.0	37.0
30-31	35.9704640169077	37.0	37.0	37.0	35.0	37.0
32-33	35.9078631988159	37.0	37.0	37.0	33.0	37.0
34-35	35.827843813749396	37.0	37.0	37.0	33.0	37.0
36-37	35.74188119123683	37.0	37.0	37.0	33.0	37.0
38-39	35.67867327765084	37.0	37.0	37.0	33.0	37.0
40-41	35.54541844100676	37.0	37.0	37.0	33.0	37.0
42-43	35.47744474031359	37.0	37.0	37.0	33.0	37.0
44-45	35.364624703013085	37.0	37.0	37.0	33.0	37.0
46-47	35.19977640690444	37.0	37.0	37.0	33.0	37.0
48-49	35.052899537067816	37.0	37.0	37.0	33.0	37.0
50-51	34.940268451679046	37.0	37.0	37.0	27.0	37.0
52-53	34.85314937348445	37.0	37.0	37.0	27.0	37.0
54-55	34.7844373606917	37.0	37.0	37.0	27.0	37.0
56-57	34.73700693173588	37.0	37.0	37.0	27.0	37.0
58-59	34.676363865396254	37.0	37.0	37.0	27.0	37.0
60-61	34.64236826727598	37.0	37.0	37.0	27.0	37.0
62-63	34.639182328097505	37.0	37.0	37.0	27.0	37.0
64-65	34.601300968903416	37.0	37.0	37.0	27.0	37.0
66-67	34.555357661475156	37.0	37.0	37.0	27.0	37.0
68-69	34.51454579809439	37.0	37.0	37.0	27.0	37.0
70-71	34.483485952825006	37.0	37.0	37.0	27.0	37.0
72-73	34.449289155909824	37.0	37.0	37.0	27.0	37.0
74-75	34.36086316032569	37.0	37.0	37.0	27.0	37.0
76-77	34.294908445800544	37.0	37.0	37.0	27.0	37.0
78-79	34.26614401634784	37.0	37.0	37.0	27.0	37.0
80-81	34.24071248867163	37.0	37.0	37.0	27.0	37.0
82-83	34.189182852963924	37.0	37.0	37.0	27.0	37.0
84-85	34.10603526402531	37.0	37.0	37.0	27.0	37.0
86-87	33.94020721726321	37.0	37.0	37.0	27.0	37.0
88-89	33.853058396638055	37.0	37.0	37.0	22.0	37.0
90-91	33.796575771466166	37.0	37.0	37.0	22.0	37.0
92-93	33.728530339028715	37.0	35.0	37.0	22.0	37.0
94-95	33.7408297088391	37.0	33.0	37.0	22.0	37.0
96-97	33.69464146374747	37.0	33.0	37.0	22.0	37.0
98-99	33.62547456672277	37.0	33.0	37.0	22.0	37.0
100-101	33.57134159356444	37.0	33.0	37.0	22.0	37.0
102-103	33.45792145898869	37.0	33.0	37.0	18.0	37.0
104-105	33.361753683687496	37.0	33.0	37.0	14.0	37.0
106-107	33.27741814708156	37.0	33.0	37.0	14.0	37.0
108-109	33.175034203795136	37.0	33.0	37.0	14.0	37.0
110-111	33.09336848771987	37.0	33.0	37.0	14.0	37.0
112-113	33.02914758194039	37.0	33.0	37.0	14.0	37.0
114-115	32.8931931823351	37.0	33.0	37.0	14.0	37.0
116-117	32.76953290387596	37.0	33.0	37.0	14.0	37.0
118-119	32.62042535174798	37.0	33.0	37.0	14.0	37.0
120-121	32.53016757235284	37.0	33.0	37.0	14.0	37.0
122-123	32.38276233698524	37.0	33.0	37.0	14.0	37.0
124-125	31.109581611479882	35.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	7.0
16	5.0
17	9.0
18	29.0
19	252.0
20	939.0
21	2257.0
22	3466.0
23	4374.0
24	3516.0
25	2383.0
26	2179.0
27	2633.0
28	3250.0
29	4387.0
30	6042.0
31	8149.0
32	11672.0
33	17355.0
34	29489.0
35	61075.0
36	122319.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	44.2233850784928	13.3288997007298	12.537496280998967	29.91021893977843
2	17.62507610589742	20.73719496406402	38.14952446935819	23.48820446068037
3	20.337945175760886	26.5982238458147	28.799171407976598	24.264659570447815
4	14.1171571834968	18.578171855262834	34.4896723783797	32.81499858286066
5	15.958947011131055	35.246713346420464	32.42843195918496	16.365907683263522
6	29.37837885123432	34.815333205031756	19.02444145074094	16.781846492992987
7	25.905201826675768	31.996220670830926	23.056707434430386	19.04187006806292
8	26.154443694079855	31.248447270169745	21.617848257618437	20.979260778131966
9	26.946642079590745	15.777484630161624	21.295580274820058	35.98029301542757
10-11	25.327079258328755	25.0147837375388	26.85951425362245	22.798622750509995
12-13	26.73792019930928	24.14298061143439	27.011550560382382	22.107548628873953
14-15	24.72680702761147	24.357126111404646	26.02952548576387	24.886541375220006
16-17	23.021166113224183	26.417051860301555	27.063337380636625	23.498444645837637
18-19	22.992998281937247	24.80991087768163	29.710238744239593	22.486852096141533
20-21	24.399158112786992	23.8905616605403	29.52308803949802	22.18719218717469
22-23	24.12373550931288	23.835058977490228	29.457952950973976	22.583252562222913
24-25	23.21869084318041	24.643178311119822	29.390770049022525	22.747360796677246
26-27	23.04635970145598	24.648077064387113	29.534408493038523	22.771154741118384
28-29	22.640463002165948	24.372347237628023	30.183493300954904	22.80369645925112
30-31	23.156056783548586	24.219436153498936	29.86682389331915	22.757683169633328
32-33	22.67440436408934	24.356601244983153	29.61033916868157	23.35865522224594
34-35	23.004585590991876	24.36924067441954	29.850605259520655	22.775568475067924
36-37	23.02798937670363	24.576520275589864	29.269875816604674	23.125614531101835
38-39	23.082400529065353	24.385643853639248	29.35892815278512	23.17302746451028
40-41	22.784683888689628	24.561590748046992	29.567058288353703	23.086667074909677
42-43	23.328428739636056	24.233999856536258	29.225138346282975	23.212433057544708
44-45	23.07190320063544	24.522983900597296	28.942009258643676	23.463103640123588
46-47	23.009089557069995	24.645559764493864	28.73157374439011	23.613776934046037
48-49	23.285391842947877	24.694241772085455	28.43455286686613	23.585813518100537
50-51	23.113017736985935	25.196562474850147	27.985877594152285	23.704542194011623
52-53	23.03446272923541	24.989940060254664	27.875480690164355	24.100116520345573
54-55	23.184574525783187	25.01058480616683	27.7304426023577	24.074398065692282
56-57	23.421464237351593	24.75567468079374	27.47255823392947	24.350302847925203
58-59	23.090623436335452	24.916458761245263	27.819669894011973	24.173247908407312
60-61	23.04635970145598	24.957048431174268	28.11779402142155	23.878797845948206
62-63	22.762931833848285	25.079867173804267	27.99969907658501	24.157501915762438
64-65	22.788475333027744	24.88129271100505	28.2019476043347	24.12828435163251
66-67	23.18543130945695	24.766109037718252	27.98905388546823	24.059405767356573
68-69	23.280450125443075	24.56619790263378	28.113595090049582	24.039756881873565
70-71	22.834313667171706	24.69741450800771	28.47417832161715	23.994093503203434
72-73	23.12718913036632	24.68236833725817	28.644759908603262	23.545682623772247
74-75	23.08388522130664	24.805635647148826	28.543943587508164	23.56653554403637
76-77	22.95399575065368	24.699588016423338	28.753093377110233	23.593322855812747
78-79	22.899302275128942	24.906573450064034	28.67845170862114	23.515672566185888
80-81	22.716918544230495	24.816909096634905	28.883924041331483	23.58224831780312
82-83	22.988974306039115	24.89493923796394	28.7173664302435	23.398720025753445
84-85	22.85794305819337	24.90543248991309	28.382506104658795	23.854118347234742
86-87	22.70760191892759	24.99268220434791	28.42284414234559	23.876871734378916
88-89	22.99734036491526	24.70830798469088	28.310298941580946	23.98405270881291
90-91	23.114936166038373	24.735484955925838	28.153860167888222	23.995718710147568
92-93	22.886059190167547	25.080077827417174	28.11786895782363	23.91599402459164
94-95	22.297414328528465	25.1108782255285	28.390599276032987	24.201108169910054
96-97	22.76818049806324	24.978742909929423	27.91624531556719	24.336831276440147
98-99	22.62856602994538	25.28613967745209	27.586279291920206	24.499015000682327
100-101	22.814018832207204	25.35769646624934	27.35113913509012	24.477145566453338
102-103	22.975152823606393	25.44657384695595	27.27993225724053	24.298341072197125
104-105	22.86351627972077	25.60893678814493	27.209790576832233	24.317756355302063
106-107	22.786894137283685	25.69891927545791	27.161055425405163	24.353131161853238
108-109	22.801550837879354	25.735965652020255	26.82876048442689	24.633723025673504
110-111	22.674308748504128	25.883703190499187	26.739413407234785	24.702574653761904
112-113	22.681227627568784	25.94134792695259	26.702054327173734	24.67537011830489
114-115	22.604597130030406	26.040722636089114	26.702054327173734	24.652625906706742
116-117	22.892263980278983	25.971835618547413	26.673408296053175	24.462492105120432
118-119	22.61789374604163	25.850720991507657	26.845167904768235	24.68621735768247
120-121	22.371323947506085	25.87465074330223	27.12611381613133	24.627911493060356
122-123	22.506447109210708	26.15934244734715	26.8455178157159	24.488692627726245
124-125	22.541263248503256	25.95324489917316	26.919698936620627	24.585792915702953
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	64.0
1	66.5
2	141.0
3	223.0
4	187.0
5	165.5
6	197.5
7	379.0
8	660.5
9	798.5
10	730.0
11	592.5
12	579.0
13	609.0
14	604.0
15	595.0
16	602.0
17	634.5
18	648.0
19	600.0
20	582.0
21	623.0
22	655.5
23	670.0
24	710.5
25	749.5
26	863.5
27	1041.0
28	1202.0
29	1473.5
30	1901.5
31	2338.5
32	2920.5
33	3554.0
34	4151.5
35	4641.5
36	5189.0
37	5830.0
38	6244.0
39	6444.5
40	6387.5
41	6372.5
42	6591.0
43	7245.0
44	8136.5
45	8785.0
46	9729.0
47	10735.5
48	11490.5
49	12210.5
50	12743.0
51	12422.5
52	11601.5
53	11219.0
54	10969.0
55	10557.5
56	9996.5
57	9126.5
58	8367.0
59	7648.0
60	6766.5
61	5632.0
62	4335.5
63	3360.5
64	2542.0
65	2018.0
66	1568.5
67	1119.0
68	885.5
69	777.0
70	653.0
71	575.0
72	528.0
73	504.5
74	383.0
75	273.5
76	235.0
77	175.0
78	121.0
79	65.5
80	37.5
81	24.5
82	16.0
83	13.5
84	7.5
85	3.5
86	1.0
87	0.0
88	1.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0321918071850714
2	3.4991094766381953E-4
3	3.4991094766381953E-4
4	0.0
5	0.0034991094766381953
6	6.998218953276391E-4
7	0.0076980408486040305
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	1.7495547383190977E-4
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	1.7495547383190977E-4
36-37	0.0
38-39	0.0
40-41	0.0033241540028062858
42-43	1.7495547383190977E-4
44-45	0.0
46-47	0.006823263479444481
48-49	0.0076980408486040305
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.00822290727009976
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.06175928226266415
76-77	0.033591450975726675
78-79	3.4991094766381953E-4
80-81	0.0
82-83	0.0
84-85	0.05056213193742193
86-87	0.18317838110200954
88-89	0.20907179122913216
90-91	0.12561803021131124
92-93	0.09919975366269286
94-95	1.7495547383190977E-4
96-97	0.0
98-99	0.0
100-101	0.0
102-103	0.0
104-105	6.998218953276391E-4
106-107	0.002274421159814827
108-109	0.002099465685982917
110-111	3.4991094766381953E-4
112-113	0.0
114-115	0.0
116-117	1.7495547383190977E-4
118-119	0.0
120-121	5.248664214957293E-4
122-123	0.0
124-125	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	285787.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.699586907615
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.5149363230438	62.03085195700857
2	9.191256617899453	13.180186053305468
3	1.9199550191080903	4.129799452537564
4	0.71800034812334	2.0592131343986897
5	0.36347870148132244	1.3030636372963564
6	0.21596084136543559	0.9290581868473624
7	0.15023421355487385	0.7540211735892408
8	0.12393052820037043	0.7108614141767273
9	0.09540416930801696	0.615639557578408
>10	0.6446293812908498	8.772040533883978
>50	0.04626158569510827	2.2070972281554635
>100	0.014357043836412913	1.8506218596418413
>500	5.317423643115893E-4	0.30919949076422193
>1k	0.0010634847286231787	1.1483463208160747
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1542	0.5395626812976098	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1470	0.5143690930658147	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	811	0.2837777785553577	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	432	0.15116152939077004	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	398	0.13926455717020017	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	367	0.12841731779262178	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.499109476638196E-4	0.0
2	0.0	0.0	0.0	3.499109476638196E-4	0.0
3	0.0	0.0	0.0	3.499109476638196E-4	0.0
4	0.0	0.0	0.0	3.499109476638196E-4	0.0
5	0.0	0.0	0.0	3.499109476638196E-4	0.0
6	0.0	0.0	0.0	3.499109476638196E-4	0.0
7	0.0	0.0	0.0	3.499109476638196E-4	0.0
8	0.0	0.0	0.0	3.499109476638196E-4	3.499109476638196E-4
9	0.0	0.0	0.0	3.499109476638196E-4	6.998218953276392E-4
10-11	0.0	0.0	0.0	6.998218953276391E-4	6.998218953276392E-4
12-13	0.0	0.0	0.0	0.0010497328429914586	6.998218953276392E-4
14-15	0.0	0.0	0.0	0.0010497328429914586	6.998218953276392E-4
16-17	0.0	0.0	0.0	0.0010497328429914586	6.998218953276392E-4
18-19	0.0	0.0	0.0	0.0015745992644871879	6.998218953276392E-4
20-21	0.0	0.0	0.0	0.0027992875813105562	0.0013996437906552783
22-23	0.0	0.0	0.0	0.005073708741125384	0.0013996437906552783
24-25	0.0	0.0	0.0	0.011197150325242225	0.0013996437906552783
26-27	0.0	0.0	0.0	0.020819701385997265	0.0013996437906552783
28-29	0.0	0.0	0.0	0.037090560452364876	0.0013996437906552783
30-31	0.0	0.0	0.0	0.067882723846781	0.0013996437906552783
32-33	0.0	0.0	0.0	0.11197150325242226	0.002099465685982917
34-35	0.0	0.0	0.0	0.16673256656181001	0.002099465685982917
36-37	0.0	0.0	0.0	0.22586751671699554	0.002099465685982917
38-39	0.0	0.0	0.0	0.31771914047874816	0.002099465685982917
40-41	0.0	0.0	0.0	0.4221675583563983	0.002099465685982917
42-43	0.0	0.0	0.0	0.506321141269547	0.002099465685982917
44-45	0.0	0.0	0.0	0.5826017278602595	0.002099465685982917
46-47	0.0	0.0	0.0	0.6889746559500607	0.002099465685982917
48-49	0.0	0.0	0.0	0.8012960701501468	0.002099465685982917
50-51	0.0	0.0	0.0	0.899796001917512	0.002099465685982917
52-53	0.0	0.0	0.0	0.9895481599932816	0.002099465685982917
54-55	0.0	0.0	0.0	1.0793003180690515	0.002099465685982917
56-57	0.0	0.0	0.0	1.180949448365391	0.002099465685982917
58-59	0.0	0.0	0.0	1.2887220202458474	0.002099465685982917
60-61	0.0	0.0	0.0	1.401918211815093	0.002099465685982917
62-63	0.0	0.0	0.0	1.5177387354918173	0.002099465685982917
64-65	0.0	0.0	0.0	1.6251613964246099	0.002099465685982917
66-67	0.0	0.0	0.0	1.7338087456742257	0.002099465685982917
68-69	0.0	0.0	0.0	1.8590768649378733	0.002099465685982917
70-71	0.0	0.0	0.0	2.0042899082183583	0.002099465685982917
72-73	0.0	0.0	0.0	2.133407047906308	0.002099465685982917
74-75	0.0	0.0	0.0	2.253426502954998	0.002099465685982917
76-77	0.0	0.0	0.0	2.3872674404364087	0.002099465685982917
78-79	0.0	0.0	0.0	2.543677634042136	0.002099465685982917
80-81	0.0	0.0	0.0	2.7161837312403994	0.002099465685982917
82-83	0.0	0.0	0.0	2.8748683460059414	0.002099465685982917
84-85	0.0	0.0	0.0	3.0265547418182073	0.002099465685982917
86-87	0.0	0.0	0.0	3.189788198903379	0.002099465685982917
88-89	0.0	0.0	0.0	3.355296077148366	0.002099465685982917
90-91	0.0	0.0	0.0	3.524128109396159	0.002099465685982917
92-93	0.0	0.0	0.0	3.7214778838785527	0.002099465685982917
94-95	0.0	0.0	0.0	3.9027317547684115	0.002099465685982917
96-97	0.0	0.0	0.0	4.096232508826503	0.002099465685982917
98-99	0.0	0.0	0.0	4.2951568825733855	0.002099465685982917
100-101	0.0	0.0	0.0	4.532746416037119	0.002099465685982917
102-103	0.0	0.0	0.0	4.766311973602718	0.002099465685982917
104-105	0.0	0.0	0.0	4.994978777901024	0.002099465685982917
106-107	0.0	0.0	0.0	5.218571873458204	0.002099465685982917
108-109	0.0	0.0	0.0	5.447763544178007	0.002099465685982917
110-111	0.0	0.0	0.0	5.698649693652966	0.002099465685982917
112-113	0.0	0.0	0.0	5.967906167880274	0.002099465685982917
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCAACG	225	0.0	39.680206	1
GTATCAA	2355	0.0	32.350742	1
GGTATCA	1715	0.0	30.193977	1
ATCAACG	2810	0.0	27.102964	3
TATCAAC	2880	0.0	26.650806	2
CCGTTAT	45	0.009541704	26.434956	22-23
CAACGCA	2990	0.0	26.267324	5
CCGCAGA	230	8.192728E-9	25.878397	1
AACGCAG	3110	0.0	25.631943	6
TCAACGC	3025	0.0	25.57002	4
ATGGGTG	415	0.0	24.37328	5
ACGCAGA	3290	0.0	24.048769	7
CATGGGT	670	0.0	23.9774	4
TGTTATC	125	0.0016306172	23.799791	2
GAGTACT	1355	0.0	23.48419	12-13
CGCAGAG	3405	0.0	23.232485	8
GTTATCA	130	0.0020485155	22.884413	3
CATGGGG	970	0.0	22.695675	4
GATTAAA	185	2.6742879E-5	22.5212	1
ATGGGAG	320	4.965841E-10	22.312304	5
>>END_MODULE