FastQCFastQC Report
Sun 1 Jan 2017
ERR1142541_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142541_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences427819
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9220.21551170004137266No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6040.1411812004609426No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4370.10214600099574819No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTACG800.005339579729.7714884
CGTGTAC800.005339579729.7714883
GTACTAG1159.968139E-425.9004171
ATGGGGG6850.024.3358765
CATGGGG12700.023.4421184
GGTATCA18850.021.4897881
CATGGGT7250.021.3533424
GTATCAA23450.021.338851
GTACAAC2255.0110793E-621.1807841
GTACAAA3801.891749E-1020.379541
GTACATG35100.020.0267031
GTGTTTA1500.004668816519.8569871
TACATGG35600.019.5711982
ACATGGG34200.019.3253543
GTACAGG2801.543729E-619.1478081
CTAGGCA2851.8369392E-618.8030454
AACGCAG24450.018.7473746
ATCAGTC1600.006792780518.6093657
TATCAAC27150.018.4243012
ATCAACG25900.018.3916533
Produced by FastQC (version 0.11.5)