FastQCFastQC Report
Sun 1 Jan 2017
ERR1142541_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142541_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences427819
Sequences flagged as poor quality0
Sequence length125
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15270.3569266442116877No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12640.2954520486467408No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8250.19283856023224774No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA5270.12318293483926614No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT4430.10354846325198272No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGC208.4888533E-489.221074
TATCACG401.7735589E-459.4807172
TAACGCA558.536014E-443.2586985
ACAACGC700.002791471533.988983
GTGTTAT1301.7370985E-632.0280761
TACAACG750.003913972531.723052
GTATCAA25150.030.74551
CGACGGA800.005366984329.7403581
GGTATCA19100.025.847641
CAACGCA31650.025.5588555
AACGCAG32700.025.2838526
ATCAACG30750.025.1463173
TCAACGC32700.024.7381574
TATCAAC32800.023.5746732
ACGCAGA35250.023.4548077
CGGGCGT1300.002053934822.8771976
CGCAGAG36450.022.3562648
GCAGAGT39400.020.682389
TACTATA2607.6957804E-720.5894782
GACACCG1450.003870728220.5105927
Produced by FastQC (version 0.11.5)