##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142541_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	427819
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	22.147571753475184	14.0	14.0	33.0	14.0	33.0
2	27.656212089692136	27.0	27.0	33.0	14.0	33.0
3	31.154329284113143	33.0	33.0	33.0	27.0	33.0
4	30.786250727527296	33.0	33.0	33.0	27.0	33.0
5	32.26892681250716	33.0	33.0	33.0	33.0	33.0
6	34.89876326203371	37.0	37.0	37.0	27.0	37.0
7	35.05611017743485	37.0	37.0	37.0	33.0	37.0
8	35.41977799022484	37.0	37.0	37.0	33.0	37.0
9	35.87352595373277	37.0	37.0	37.0	33.0	37.0
10-11	35.99632438017012	37.0	37.0	37.0	33.0	37.0
12-13	36.071866840883644	37.0	37.0	37.0	37.0	37.0
14-15	36.06826251288512	37.0	37.0	37.0	37.0	37.0
16-17	36.075537785839344	37.0	37.0	37.0	37.0	37.0
18-19	36.06225763699135	37.0	37.0	37.0	37.0	37.0
20-21	36.090093006621956	37.0	37.0	37.0	37.0	37.0
22-23	36.07734111855714	37.0	37.0	37.0	37.0	37.0
24-25	36.06303600354356	37.0	37.0	37.0	37.0	37.0
26-27	36.025079531297116	37.0	37.0	37.0	37.0	37.0
28-29	35.991435630488596	37.0	37.0	37.0	35.0	37.0
30-31	35.93053487573016	37.0	37.0	37.0	33.0	37.0
32-33	35.87498685191635	37.0	37.0	37.0	33.0	37.0
34-35	35.81355549893763	37.0	37.0	37.0	33.0	37.0
36-37	35.74885290274625	37.0	37.0	37.0	33.0	37.0
38-39	35.695913458729045	37.0	37.0	37.0	33.0	37.0
40-41	35.576269403649675	37.0	37.0	37.0	33.0	37.0
42-43	35.53615197081009	37.0	37.0	37.0	33.0	37.0
44-45	35.465336976618616	37.0	37.0	37.0	33.0	37.0
46-47	35.35104915863951	37.0	37.0	37.0	33.0	37.0
48-49	35.2217199329623	37.0	37.0	37.0	33.0	37.0
50-51	35.133929301877664	37.0	37.0	37.0	33.0	37.0
52-53	35.05174384494377	37.0	37.0	37.0	33.0	37.0
54-55	34.98337497867089	37.0	37.0	37.0	30.0	37.0
56-57	34.929402387458246	37.0	37.0	37.0	27.0	37.0
58-59	34.87699003550567	37.0	37.0	37.0	27.0	37.0
60-61	34.847801289797786	37.0	37.0	37.0	27.0	37.0
62-63	34.82677487442119	37.0	37.0	37.0	27.0	37.0
64-65	34.80136342705677	37.0	37.0	37.0	27.0	37.0
66-67	34.75118215881015	37.0	37.0	37.0	27.0	37.0
68-69	34.70641556359114	37.0	37.0	37.0	27.0	37.0
70-71	34.666038675234155	37.0	37.0	37.0	27.0	37.0
72-73	34.62980255668869	37.0	37.0	37.0	27.0	37.0
74-75	34.517112376963155	37.0	37.0	37.0	27.0	37.0
76-77	34.451669981931616	37.0	37.0	37.0	27.0	37.0
78-79	34.436688178879386	37.0	37.0	37.0	27.0	37.0
80-81	34.392846040030946	37.0	37.0	37.0	27.0	37.0
82-83	34.33985867855331	37.0	37.0	37.0	27.0	37.0
84-85	34.259434480469544	37.0	37.0	37.0	27.0	37.0
86-87	34.08839836472901	37.0	37.0	37.0	27.0	37.0
88-89	33.99080452247328	37.0	37.0	37.0	27.0	37.0
90-91	33.95091382103179	37.0	37.0	37.0	27.0	37.0
92-93	33.89195547649824	37.0	37.0	37.0	27.0	37.0
94-95	33.87965588251106	37.0	33.0	37.0	24.5	37.0
96-97	33.846465444498726	37.0	33.0	37.0	22.0	37.0
98-99	33.765033811027564	37.0	33.0	37.0	22.0	37.0
100-101	33.706776697622125	37.0	33.0	37.0	22.0	37.0
102-103	33.58733950572555	37.0	33.0	37.0	22.0	37.0
104-105	33.48372091936076	37.0	33.0	37.0	22.0	37.0
106-107	33.409514303946295	37.0	33.0	37.0	22.0	37.0
108-109	33.298733810326326	37.0	33.0	37.0	18.0	37.0
110-111	33.20878923095982	37.0	33.0	37.0	14.0	37.0
112-113	33.15278891306838	37.0	33.0	37.0	14.0	37.0
114-115	33.01058625259748	37.0	33.0	37.0	14.0	37.0
116-117	32.88553921167596	37.0	33.0	37.0	14.0	37.0
118-119	32.721342436871666	37.0	33.0	37.0	14.0	37.0
120-121	32.63120618766347	37.0	33.0	37.0	14.0	37.0
122-123	32.49923098319617	37.0	33.0	37.0	14.0	37.0
124-125	31.148182993275192	35.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	5.0
16	8.0
17	9.0
18	57.0
19	284.0
20	1077.0
21	2577.0
22	3928.0
23	4919.0
24	4084.0
25	3192.0
26	3312.0
27	3929.0
28	5265.0
29	7165.0
30	9489.0
31	13160.0
32	18726.0
33	28343.0
34	46749.0
35	95116.0
36	176425.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	44.41225707498644	12.983745768101304	11.963413949834464	30.640583207077793
2	17.746289902972986	20.32588547960703	37.13088946493727	24.796935152482707
3	20.355246180601007	25.67739401985901	28.25116405183537	25.716195747704624
4	14.515718095736748	17.986578436207836	33.67171630993481	33.825987158120604
5	16.68579496181743	34.53003999410957	32.280692732572405	16.503472311500587
6	31.08894644326427	34.13190029171965	18.41760789887052	16.36154536614556
7	27.113941153507206	30.856422879396717	22.68537673770603	19.344259229390055
8	26.367926623174753	31.314177257204566	21.280027301265257	21.037868818355427
9	26.682078168571287	15.573174636937583	20.723249785540148	37.021497408950985
10-11	25.637243787676567	24.646404203646867	26.843244456183573	22.873107552492993
12-13	26.699375203064847	23.428248862252495	26.768563341039087	23.103812593643575
14-15	24.78396237661254	23.960717032202872	25.03418501749572	26.221135573688876
16-17	24.007465774077357	25.921943625692172	26.06931903445149	24.001271565778985
18-19	23.99566171675405	24.79564956208116	27.99092606920216	23.21776265196263
20-21	24.999766255744266	23.911102384658896	27.815449560327053	23.27368179926978
22-23	24.56669759875087	23.821522653271593	27.870431187020678	23.741348560956855
24-25	23.941783791743703	24.59836987137084	27.815735159027533	23.644111177857926
26-27	23.90122925816759	24.635184505596992	27.87101554629411	23.592570689941304
28-29	23.58625960978825	24.435216762228887	28.37590195853854	23.60262166944432
30-31	24.00980321117108	24.215263931709437	28.24103183823065	23.533901018888827
32-33	23.56814447231189	24.26341513584016	28.028792550120496	24.13964784172746
34-35	23.860468867054603	24.55048110355209	27.95145604970332	23.637593979689985
36-37	23.89050496881271	24.594191228289567	27.692233973051657	23.82306982984607
38-39	23.86289528983051	24.249039897713754	27.71908213520204	24.168982677253698
40-41	23.64163146810157	24.4276093882579	28.099207360259555	23.83155178338098
42-43	24.099210180006033	24.424698295307127	27.715108492142704	23.760983032544136
44-45	23.878205502794405	24.51691018865455	27.496090636460746	24.1087936720903
46-47	23.83411816544471	24.546836886992356	27.39794552809325	24.22109941946968
48-49	24.098731608795052	24.821406030117863	27.033727143945807	24.04613521714128
50-51	23.813575367152932	25.194416330270514	26.76949831587657	24.222509986699983
52-53	23.913500802909642	24.672583499096582	26.95251964031518	24.4613960576786
54-55	24.065434214001716	24.787819147817185	26.814493979930766	24.332252658250333
56-57	24.00349213101802	24.62513352609398	26.545922457861852	24.825451885026144
58-59	23.885568429639637	24.582475299133513	26.891395660314295	24.64056061091256
60-61	23.906605363483155	24.73394122280684	27.024629574656572	24.33482383905343
62-63	23.440637278849234	24.93507768472181	26.960934413852588	24.66335062257637
64-65	23.553652362330798	24.522286293970115	27.230557782613673	24.69350356108541
66-67	23.83215419877485	24.6733468058174	27.006876109639062	24.487622885768687
68-69	23.945173075529606	24.477290629915924	26.997164688805313	24.58037160574916
70-71	23.544770101374645	24.44257968907412	27.407618642463284	24.605031567087952
72-73	23.800602591282765	24.555244156991627	27.35970118204194	24.284452069683674
74-75	23.91250700278474	24.573546490888486	27.23980926695025	24.27413723937653
76-77	23.791029245851043	24.471113657222023	27.383305956145836	24.354551140781098
78-79	23.854096665049934	24.587937613585233	27.37662671583093	24.181339005533903
80-81	23.585792122369504	24.57359303817736	27.5897049920644	24.250909847388733
82-83	23.750932053041122	24.600473564755188	27.34485845649679	24.3037359257069
84-85	23.69488290724027	24.71881599842832	27.028980253387186	24.55732084094423
86-87	23.5092617424513	24.679203034586465	27.192878699690475	24.61865652327176
88-89	23.724957769973457	24.36198102753093	27.158182785143463	24.754878417352145
90-91	23.98129303252068	24.510762006972982	26.988288249276426	24.519656711229914
92-93	23.674323750375873	24.79720408996615	26.852717459344287	24.675754700313686
94-95	23.197894436665973	24.818205830035602	27.111465362688424	24.87243437061
96-97	23.63125527384244	24.65774077355143	26.854931641652197	24.85607231095393
98-99	23.380448273685833	24.816803367779364	26.73022937270201	25.072518985832794
100-101	23.59806366711156	24.98696878820249	26.325034652504915	25.08993289218104
102-103	23.761099904398822	24.836554711221336	26.633810092585886	24.76853529179396
104-105	23.648065860551224	24.83363058158588	26.460626488676127	25.057677069186774
106-107	23.564810973108358	25.00598981556043	26.502684021743324	24.92651518958789
108-109	23.77280823550862	25.013791297924758	26.17436408091518	25.039036385651443
110-111	23.555316097831206	25.12537442864205	26.166937614899776	25.152371858626964
112-113	23.612088289673906	25.208557824687546	26.129624911469566	25.049728974168982
114-115	23.603206028717754	25.040028610230024	26.26741682814461	25.089348532907607
116-117	23.614859139703263	25.3582427086316	26.060995634820923	24.965902516844217
118-119	23.341529946075326	25.040963585067516	26.331579476367345	25.285926992489816
120-121	23.218330353073174	25.057326180708948	26.5256010191321	25.198742447085777
122-123	23.32914152947859	25.156549849352178	26.39118412225731	25.123124498911924
124-125	23.42252214137287	25.097412690880955	26.3480584078781	25.132006759868077
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	58.0
1	55.5
2	158.5
3	245.0
4	193.5
5	179.0
6	244.0
7	425.0
8	684.0
9	796.5
10	712.0
11	635.5
12	622.5
13	626.0
14	629.5
15	637.0
16	626.0
17	637.0
18	663.5
19	644.5
20	641.0
21	647.0
22	663.5
23	718.5
24	787.5
25	877.0
26	1024.0
27	1207.0
28	1430.5
29	1783.5
30	2305.5
31	2792.0
32	3431.0
33	4192.5
34	4923.5
35	5731.0
36	6557.0
37	7345.0
38	8077.5
39	8757.5
40	9039.0
41	9283.5
42	9926.5
43	11059.0
44	12445.0
45	13447.0
46	14742.5
47	16072.5
48	17055.5
49	18127.5
50	18847.5
51	18373.0
52	17633.5
53	17817.0
54	17518.0
55	16784.5
56	16025.0
57	14892.0
58	14163.0
59	12993.0
60	11303.5
61	9390.0
62	7540.0
63	5986.5
64	4535.5
65	3690.5
66	2913.5
67	2090.5
68	1705.0
69	1470.5
70	1264.5
71	1183.0
72	1069.0
73	1023.0
74	795.5
75	641.0
76	561.0
77	378.5
78	245.0
79	150.5
80	89.5
81	72.5
82	57.0
83	35.0
84	23.0
85	13.5
86	7.0
87	3.0
88	1.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.026880526577828474
2	0.0
3	7.012311281172645E-4
4	0.0
5	0.001402462256234529
6	0.0025711808030966367
7	0.006544823862427803
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	2.3374370937242152E-4
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	1.1687185468621076E-4
36-37	1.1687185468621076E-4
38-39	0.0
40-41	0.0018699496749793722
42-43	0.0
44-45	0.0
46-47	0.004791746042134641
48-49	0.0077135424092899104
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.007830414263976121
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.07257742176013687
76-77	0.035412171969921864
78-79	3.5061556405863224E-4
80-81	0.0
82-83	0.0
84-85	0.058903414761850216
86-87	0.20440887384618264
88-89	0.2314062722786973
90-91	0.13989561005939427
92-93	0.11231385235344854
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
102-103	0.0
104-105	0.001402462256234529
106-107	0.0022205652390380045
108-109	0.0030386682218414797
110-111	1.1687185468621076E-4
112-113	0.0
114-115	0.0
116-117	3.5061556405863224E-4
118-119	0.0
120-121	9.349748374896861E-4
122-123	0.0
124-125	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	427819.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.08386072058636
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.43164068229447	67.39568412926339
2	9.154649182103638	14.11351404998213
3	1.7965806445221846	4.15462116526948
4	0.5903249990046519	1.8201812001261946
5	0.27502266146020454	1.059990426550169
6	0.1493863749182966	0.6909167110653162
7	0.10829731280530942	0.5843582483688772
8	0.07378393630001868	0.4550040535333806
9	0.047647868881240486	0.33055935196268726
>10	0.32642059430525805	4.905106462440831
>50	0.031173056675120703	1.6522189909085656
>100	0.013702442481535232	1.743847564002316
>500	6.851221240767616E-4	0.35700816270913555
>1k	6.851221240767616E-4	0.7369894838174537
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1527	0.3569266442116877	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1264	0.2954520486467408	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	825	0.19283856023224774	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	527	0.12318293483926614	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	443	0.10354846325198272	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	1.1687185468621076E-4	0.0	0.0	0.0	0.0
12-13	2.3374370937242152E-4	0.0	0.0	0.0	0.0
14-15	2.3374370937242152E-4	0.0	0.0	2.3374370937242152E-4	0.0
16-17	2.3374370937242152E-4	0.0	0.0	2.3374370937242152E-4	0.0
18-19	2.3374370937242152E-4	0.0	0.0	8.181029828034752E-4	0.0
20-21	2.3374370937242152E-4	0.0	0.0	0.0016362059656069507	0.0
22-23	2.3374370937242152E-4	0.0	0.0	0.003739899349958744	2.3374370937242152E-4
24-25	2.3374370937242152E-4	0.0	0.0	0.005843592734310538	2.3374370937242152E-4
26-27	2.3374370937242152E-4	0.0	0.0	0.01227154474205213	2.3374370937242152E-4
28-29	2.3374370937242152E-4	0.0	0.0	0.025244320612221524	2.3374370937242152E-4
30-31	2.3374370937242152E-4	0.0	0.0	0.04791746042134641	2.3374370937242152E-4
32-33	2.3374370937242152E-4	0.0	0.0	0.0813428108616027	2.3374370937242152E-4
34-35	2.3374370937242152E-4	0.0	0.0	0.12236483185646266	2.3374370937242152E-4
36-37	2.3374370937242152E-4	0.0	0.0	0.17355470420902297	2.3374370937242152E-4
38-39	2.3374370937242152E-4	0.0	0.0	0.24414530443949425	2.3374370937242152E-4
40-41	2.3374370937242152E-4	0.0	0.0	0.3296955020698006	2.3374370937242152E-4
42-43	2.3374370937242152E-4	0.0	0.0	0.40461036092366165	3.506155640586323E-4
44-45	2.3374370937242152E-4	0.0	0.0	0.4825638879993642	4.6748741874484305E-4
46-47	2.3374370937242152E-4	0.0	0.0	0.5820218363373295	4.6748741874484305E-4
48-49	2.3374370937242152E-4	0.0	0.0	0.6837003499143329	4.6748741874484305E-4
50-51	2.3374370937242152E-4	0.0	0.0	0.7783665522101636	4.6748741874484305E-4
52-53	2.3374370937242152E-4	0.0	0.0	0.8630986468576665	7.012311281172646E-4
54-55	2.3374370937242152E-4	0.0	0.0	0.9482982289239141	7.012311281172646E-4
56-57	2.3374370937242152E-4	0.0	0.0	1.0478730491165655	9.34974837489686E-4
58-59	4.6748741874484305E-4	0.0	0.0	1.1585506955044074	0.0011687185468621075
60-61	4.6748741874484305E-4	0.0	0.0	1.2646703395594865	0.0011687185468621075
62-63	4.6748741874484305E-4	0.0	0.0	1.3656476220083729	0.0011687185468621075
64-65	4.6748741874484305E-4	0.0	0.0	1.460664439868262	0.0011687185468621075
66-67	4.6748741874484305E-4	0.0	0.0	1.567602186906145	0.0011687185468621075
68-69	4.6748741874484305E-4	0.0	0.0	1.6864608631220213	0.0011687185468621075
70-71	4.6748741874484305E-4	0.0	0.0	1.8158380062596566	0.0011687185468621075
72-73	4.6748741874484305E-4	0.0	0.0	1.93539791360365	0.0011687185468621075
74-75	4.6748741874484305E-4	0.0	0.0	2.0464261755555504	0.0011687185468621075
76-77	4.6748741874484305E-4	0.0	0.0	2.1767382935306756	0.0011687185468621075
78-79	4.6748741874484305E-4	0.0	0.0	2.314647082060404	0.0011687185468621075
80-81	4.6748741874484305E-4	0.0	0.0	2.4544258202651124	0.0011687185468621075
82-83	4.6748741874484305E-4	0.0	0.0	2.6092810277243412	0.0011687185468621075
84-85	4.6748741874484305E-4	0.0	0.0	2.749994740766539	0.0011687185468621075
86-87	4.6748741874484305E-4	0.0	0.0	2.8983051243633406	0.0012855904015483183
88-89	4.6748741874484305E-4	0.0	0.0	3.059354540120939	0.0014024622562345292
90-91	4.6748741874484305E-4	0.0	0.0	3.2297537042534343	0.0014024622562345292
92-93	4.6748741874484305E-4	0.0	0.0	3.4100869760342576	0.0014024622562345292
94-95	4.6748741874484305E-4	0.0	0.0	3.587147835883867	0.0016362059656069507
96-97	4.6748741874484305E-4	0.0	0.0	3.7617543867850656	0.0016362059656069507
98-99	4.6748741874484305E-4	0.0	0.0	3.94945058541112	0.0016362059656069507
100-101	4.6748741874484305E-4	0.0	0.0	4.166131003999355	0.0016362059656069507
102-103	4.6748741874484305E-4	0.0	0.0	4.3935636332187205	0.0018699496749793722
104-105	4.6748741874484305E-4	0.0	0.0	4.617606978652187	0.0021036933843517937
106-107	4.6748741874484305E-4	0.0	0.0	4.840832221102849	0.0021036933843517937
108-109	4.6748741874484305E-4	0.0	0.0	5.049682225427108	0.002337437093724215
110-111	4.6748741874484305E-4	0.0	0.0	5.281789728833923	0.002337437093724215
112-113	4.6748741874484305E-4	0.0	0.0	5.551179353885638	0.002337437093724215
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAACGC	20	8.4888533E-4	89.22107	4
TATCACG	40	1.7735589E-4	59.480717	2
TAACGCA	55	8.536014E-4	43.258698	5
ACAACGC	70	0.0027914715	33.98898	3
GTGTTAT	130	1.7370985E-6	32.028076	1
TACAACG	75	0.0039139725	31.72305	2
GTATCAA	2515	0.0	30.7455	1
CGACGGA	80	0.0053669843	29.740358	1
GGTATCA	1910	0.0	25.84764	1
CAACGCA	3165	0.0	25.558855	5
AACGCAG	3270	0.0	25.283852	6
ATCAACG	3075	0.0	25.146317	3
TCAACGC	3270	0.0	24.738157	4
TATCAAC	3280	0.0	23.574673	2
ACGCAGA	3525	0.0	23.454807	7
CGGGCGT	130	0.0020539348	22.877197	6
CGCAGAG	3645	0.0	22.356264	8
GCAGAGT	3940	0.0	20.68238	9
TACTATA	260	7.6957804E-7	20.589478	2
GACACCG	145	0.0038707282	20.510592	7
>>END_MODULE