FastQCFastQC Report
Sun 1 Jan 2017
ERR1142536_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142536_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences709784
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15650.22048961374164533No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11280.15892158741250859No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA7510.10580683700956912No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33150.025.5270331
GGTATCA25350.024.4484271
ATCAACG37100.022.797913
AACGCAG38000.022.5586936
TCAACGC37850.022.3461694
GTTTAGA2454.275953E-721.8913211
CAACGCA38300.021.772585
TATCAAC39150.021.7593672
GTCTTAA2755.4173142E-821.6701971
CATGGGG23750.021.5682754
GTACATG54850.020.3170431
ACGCAGA41650.020.1600957
CGCAGAG42750.019.7820558
TACATGG55750.019.768282
ACATGGG54750.019.5825373
GCAGAGT45100.018.7579129
GTCTTAT2351.6718991E-417.7511181
CATGGGT9750.017.716344
GTAGTAT1700.00960005117.5273671
AATAGAC1700.0096392117.5149787
Produced by FastQC (version 0.11.5)