##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142536_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	709784
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.444995942427557	33.0	33.0	33.0	27.0	33.0
2	31.499540705341342	33.0	33.0	33.0	27.0	33.0
3	31.60234662939711	33.0	33.0	33.0	27.0	33.0
4	31.568232025517624	33.0	33.0	33.0	27.0	33.0
5	31.60188310810049	33.0	33.0	33.0	27.0	33.0
6	35.062778253665904	37.0	37.0	37.0	33.0	37.0
7	35.037710063906765	37.0	37.0	37.0	33.0	37.0
8	35.044784046977675	37.0	37.0	37.0	33.0	37.0
9	34.999234978528676	37.0	37.0	37.0	33.0	37.0
10-11	35.05513226559066	37.0	37.0	37.0	33.0	37.0
12-13	35.002252798034334	37.0	37.0	37.0	33.0	37.0
14-15	34.98460447121941	37.0	37.0	37.0	33.0	37.0
16-17	34.95061314428051	37.0	37.0	37.0	33.0	37.0
18-19	34.9923871205888	37.0	37.0	37.0	33.0	37.0
20-21	34.97153570663751	37.0	37.0	37.0	30.0	37.0
22-23	35.05247159699289	37.0	37.0	37.0	33.0	37.0
24-25	35.10537360661835	37.0	37.0	37.0	33.0	37.0
26-27	35.15530499419542	37.0	37.0	37.0	33.0	37.0
28-29	35.186539144303055	37.0	37.0	37.0	33.0	37.0
30-31	35.12325017188328	37.0	37.0	37.0	33.0	37.0
32-33	35.14955535768628	37.0	37.0	37.0	33.0	37.0
34-35	35.203819753615186	37.0	37.0	37.0	33.0	37.0
36-37	35.194476770397756	37.0	37.0	37.0	33.0	37.0
38-39	35.17472357787722	37.0	37.0	37.0	33.0	37.0
40-41	35.13886900803625	37.0	37.0	37.0	33.0	37.0
42-43	35.14386278078965	37.0	37.0	37.0	33.0	37.0
44-45	35.13790392570134	37.0	37.0	37.0	33.0	37.0
46-47	35.117737931539736	37.0	37.0	37.0	33.0	37.0
48-49	35.11351833797325	37.0	37.0	37.0	33.0	37.0
50-51	35.04395351261792	37.0	37.0	37.0	33.0	37.0
52-53	35.06870470452983	37.0	37.0	37.0	33.0	37.0
54-55	35.042410085321734	37.0	37.0	37.0	33.0	37.0
56-57	35.01582594141316	37.0	37.0	37.0	33.0	37.0
58-59	34.98347032336598	37.0	37.0	37.0	27.0	37.0
60-61	34.97411677355365	37.0	37.0	37.0	27.0	37.0
62-63	34.95364716589836	37.0	37.0	37.0	27.0	37.0
64-65	34.91384984727748	37.0	37.0	37.0	27.0	37.0
66-67	34.867691438522144	37.0	37.0	37.0	27.0	37.0
68-69	34.82944600047338	37.0	37.0	37.0	27.0	37.0
70-71	34.78998117737227	37.0	37.0	37.0	27.0	37.0
72-73	34.73695025528893	37.0	37.0	37.0	27.0	37.0
74-75	34.6717050539319	37.0	37.0	37.0	27.0	37.0
76-77	34.62030843186096	37.0	37.0	37.0	27.0	37.0
78-79	34.56347283117117	37.0	37.0	37.0	27.0	37.0
80-81	34.513397033463704	37.0	37.0	37.0	27.0	37.0
82-83	34.47576868455755	37.0	37.0	37.0	27.0	37.0
84-85	34.41589694893094	37.0	37.0	37.0	27.0	37.0
86-87	34.36015393415462	37.0	37.0	37.0	27.0	37.0
88-89	34.31827499633691	37.0	37.0	37.0	27.0	37.0
90-91	34.23247776788431	37.0	37.0	37.0	27.0	37.0
92-93	34.165922308758724	37.0	37.0	37.0	27.0	37.0
94-95	34.10906627931878	37.0	37.0	37.0	27.0	37.0
96-97	34.02921522604059	37.0	37.0	37.0	27.0	37.0
98-99	33.92792384725494	37.0	37.0	37.0	22.0	37.0
100-101	33.83904117308928	37.0	37.0	37.0	22.0	37.0
102-103	33.739381276557374	37.0	33.0	37.0	22.0	37.0
104-105	33.60902964845643	37.0	33.0	37.0	22.0	37.0
106-107	33.537529022914015	37.0	33.0	37.0	22.0	37.0
108-109	33.43873488272489	37.0	33.0	37.0	22.0	37.0
110-111	33.356177372271	37.0	33.0	37.0	22.0	37.0
112-113	33.22593000123982	37.0	33.0	37.0	22.0	37.0
114-115	33.12749723859653	37.0	33.0	37.0	18.0	37.0
116-117	32.92347883299708	37.0	33.0	37.0	14.0	37.0
118-119	32.83695039617686	37.0	33.0	37.0	14.0	37.0
120-121	32.75240002592338	37.0	33.0	37.0	14.0	37.0
122-123	32.66686555346415	37.0	33.0	37.0	14.0	37.0
124-125	31.008272234933443	35.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	1380.0
1	93.0
2	47.0
3	31.0
4	39.0
5	27.0
6	26.0
7	43.0
8	31.0
9	30.0
10	31.0
11	31.0
12	33.0
13	27.0
14	78.0
15	251.0
16	370.0
17	638.0
18	1296.0
19	2254.0
20	3481.0
21	4265.0
22	4861.0
23	5203.0
24	5792.0
25	6731.0
26	8071.0
27	9551.0
28	11453.0
29	13990.0
30	16932.0
31	21047.0
32	27116.0
33	37184.0
34	57403.0
35	112225.0
36	357723.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	44.39832381211077	18.93294399597081	11.777172267635482	24.891559924282937
2	17.636802271647397	22.2731463674005	35.43043079847049	24.659620562481617
3	20.039478577357226	26.39889482607226	27.880472668296523	25.681153928273986
4	14.883386211069826	18.390424322627712	33.629250719646194	33.096938746656264
5	15.8161944911521	36.8852679259585	31.128653391164402	16.16988419172499
6	30.95461849035433	35.061748982155756	18.016514443425383	15.967118084064538
7	27.294527952338086	32.175747649618245	21.29326461772823	19.23645978031544
8	25.94674409896639	33.292638986032514	20.341097758278405	20.419519156722693
9	26.66716598034588	17.57890333758221	19.408652446589922	36.345278235481985
10-11	25.457428227433827	26.38450820459774	25.748955701389946	22.409107866578488
12-13	26.24787341491221	25.050624563992628	25.247397738380734	23.454104282714425
14-15	24.736593103438523	25.727493817478237	23.032359852821685	26.503553226261555
16-17	24.59368474036119	27.547361619520665	23.84531530346925	24.013638336648892
18-19	24.674855619002383	26.62554520568859	25.161980247845506	23.537618927463523
20-21	25.97604983398566	25.759997964575128	24.574645673520674	23.68930652791854
22-23	25.693319133509206	25.50406666661955	24.488619057681014	24.313995142190226
24-25	24.856126417980576	26.10546843259933	24.55703894213615	24.48136620728394
26-27	24.81868440912033	25.97518958290348	24.585998422851134	24.620127585125058
28-29	24.506278208131853	25.99154144634767	24.94043968652764	24.561740658992832
30-31	25.030805057681764	25.90203709041991	24.848871517474098	24.218286334424228
32-33	24.51057486125683	26.000840760641097	24.66629221024668	24.82229216785539
34-35	24.792804604781672	25.982994860028313	24.836391225596437	24.387809309593578
36-37	24.704970622456354	26.153537491425517	24.535282369870938	24.60620951624719
38-39	24.76495029166649	25.88428843689648	24.5818552220946	24.768906049342426
40-41	24.62879375012805	26.171480084467657	24.801388672036442	24.398337493367848
42-43	24.778978614525283	25.93800310401268	24.79995931016603	24.483058971296007
44-45	24.545245840264755	26.001109027270065	24.722125094037743	24.731520038427444
46-47	24.57133250357076	26.048870284374985	24.80973619483692	24.570061017217338
48-49	24.666195790365872	26.27009464613646	24.691693742053964	24.372015821443707
50-51	24.269613078587938	26.521097869021986	24.69216381010505	24.517125242285022
52-53	24.39569847508342	26.089861861827924	24.76322399631556	24.751215666773092
54-55	24.43760180950726	25.9993049050814	24.862287851238385	24.700805434172956
56-57	24.589515471090376	25.80424594106823	24.553702925397594	25.0525356624438
58-59	24.542998506150212	25.77325267250787	24.872210509215606	24.811538312126316
60-61	24.620984983483314	25.97770512366855	24.90675153495343	24.494558357894707
62-63	24.126530298011257	26.20214872098764	24.843782997685437	24.827537983315665
64-65	24.297115574766224	25.823445489730766	24.946817526909058	24.93262140859395
66-67	24.58456603403137	25.855109966672057	24.707100426645688	24.85322357265089
68-69	24.7009422367882	25.73719080648123	24.572673720493295	24.98919323623727
70-71	24.32716858130657	25.683602371728544	24.963537675381133	25.025691371583754
72-73	24.60263822653099	25.868080199035752	24.85072671636911	24.678554858064146
74-75	24.69710807342158	25.597184286419605	25.03607580050135	24.66963183965746
76-77	24.645677412791642	25.50084138809962	24.943730846234384	24.909750352874354
78-79	24.635215141485244	25.66984939601601	25.01825866703961	24.676676795459137
80-81	24.396618463955335	25.633967371341708	25.23394985335752	24.73546431134544
82-83	24.60008023822843	25.655891011623243	25.063498386759562	24.680530363388765
84-85	24.427734330854598	25.852253463824788	24.856968899034875	24.86304330628574
86-87	24.22666578147049	25.824397795861522	24.959989603653412	24.98894681901458
88-89	24.45305526366338	25.356131995525576	25.065040168129894	25.125772572681154
90-91	24.688919738397423	25.552078316953754	24.88373768000712	24.875264264641707
92-93	24.42541093525241	25.89169260058795	24.961884726985893	24.721011737173743
94-95	24.014556844491864	25.90936147167043	25.123972544220933	24.952109139616773
96-97	24.299595936346734	25.877442262248113	24.931214570296696	24.891747231108454
98-99	24.065627431515118	25.92498188940463	24.939949078513138	25.06944160056711
100-101	24.26686377457407	26.183159275473127	24.687059428175274	24.86291752177753
102-103	24.499659709298548	26.026661282260548	24.96441773578192	24.509261272658982
104-105	24.312659214532225	26.14923944171124	24.870651431024452	24.667449912732078
106-107	24.2089210827297	26.15890766862936	24.98736250547154	24.6448087431694
108-109	24.328224490674817	26.096186688675004	24.88278654902844	24.69280227162174
110-111	24.055014600147977	26.451895760026662	24.723740913737693	24.76934872608767
112-113	24.16046402775356	26.49408704897467	24.70301108320914	24.642437840062634
114-115	24.11420810621173	26.499496697118964	24.84911501988505	24.537180176784254
116-117	24.19906742298827	26.556630580195623	24.68003995750103	24.564262039315075
118-119	23.97494377596788	26.33790608010142	24.959376107362843	24.727774036567858
120-121	23.970473943050035	26.318271241883124	25.056418430279386	24.65483638478746
122-123	24.050482813723047	26.452446677333906	24.997900196146382	24.499170312796664
124-125	24.02135231316726	26.364869614808562	24.885058039161436	24.728720032862743
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2080.0
1	1221.0
2	294.5
3	220.5
4	199.0
5	175.5
6	151.5
7	126.5
8	115.0
9	123.0
10	131.0
11	124.5
12	123.5
13	137.5
14	145.0
15	148.5
16	165.5
17	180.0
18	208.0
19	241.0
20	313.0
21	389.5
22	443.0
23	611.0
24	822.5
25	1029.5
26	1346.5
27	1718.0
28	2207.0
29	3026.0
30	4039.0
31	4950.0
32	6023.0
33	7262.5
34	8364.5
35	9524.0
36	10794.5
37	12075.5
38	13226.5
39	14254.0
40	15073.5
41	15731.0
42	16681.5
43	18459.0
44	20553.5
45	22280.5
46	24502.5
47	26646.0
48	28644.5
49	30663.5
50	32007.0
51	31533.5
52	30590.5
53	30704.0
54	30368.0
55	29084.5
56	27576.5
57	25804.0
58	23969.5
59	21761.0
60	18936.0
61	16061.5
62	13150.5
63	10141.5
64	7782.5
65	6346.0
66	4918.5
67	3780.5
68	3020.0
69	2491.5
70	2159.0
71	1963.0
72	1754.5
73	1650.0
74	1353.5
75	1084.0
76	850.0
77	608.0
78	470.0
79	291.0
80	199.0
81	160.0
82	110.5
83	65.0
84	38.0
85	27.0
86	19.0
87	9.5
88	4.5
89	3.5
90	3.5
91	2.0
92	1.5
93	1.0
94	0.0
95	0.0
96	0.5
97	1.5
98	1.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.4144922962478726
2	0.3708170372958534
3	0.3613775458449331
4	0.3510927268013931
5	0.37574811491946847
6	0.30445882127520485
7	0.3005139591763128
8	0.29149713152170237
9	0.30234550229365553
10-11	0.3003730712442095
12-13	0.33397484305084363
14-15	0.387512257250093
16-17	0.35405137337556214
18-19	0.3335521792545338
20-21	0.3261555628191112
22-23	0.31889983431579183
24-25	0.3003026272781579
26-27	0.30685391612096075
28-29	0.29558288155269774
30-31	0.2969917608737306
32-33	0.29466710999402634
34-35	0.28149408834236894
36-37	0.2839596271541765
38-39	0.27543590726192757
40-41	0.28987692030251455
42-43	0.2804374288515943
44-45	0.2756472391600825
46-47	0.27487235553351447
48-49	0.26543286408259414
50-51	0.27332258828037825
52-53	0.27360436414458483
54-55	0.27712656244716705
56-57	0.2721250408575003
58-59	0.2650806442523359
60-61	0.26254466147447675
62-63	0.2640944287276129
64-65	0.2597973467984626
66-67	0.25634559246193206
68-69	0.26684174340362704
70-71	0.26226288561027017
72-73	0.24944208378887095
74-75	0.2676166270301951
76-77	0.28529806250915773
78-79	0.26782795892835004
80-81	0.27332258828037825
82-83	0.26655996753942046
84-85	0.2671235192678336
86-87	0.25965645886635935
88-89	0.24796276050178648
90-91	0.23767794145824644
92-93	0.2443701182331526
94-95	0.23436707505381918
96-97	0.2259137991276219
98-99	0.23035176898887547
100-101	0.21731963526932135
102-103	0.22063050167374865
104-105	0.22823845000732615
106-107	0.22175760513057494
108-109	0.22084183357190357
110-111	0.22147582926636836
112-113	0.21844673872614767
114-115	0.20611904466710998
116-117	0.21576986801618522
118-119	0.20527371707449027
120-121	0.2057668248368518
122-123	0.1950593419970019
124-125	0.19484801009884697
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	709784.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.40364086320574
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.48871876921015	70.3441152350158
2	8.99434597813255	14.463563276503768
3	1.8694057638310762	4.5092108898804195
4	0.6643138631229198	2.1365301308393625
5	0.3272439965959959	1.3155804388472288
6	0.1818878342750731	0.8774666462663557
7	0.11893817119802372	0.6694143401352665
8	0.07961986739348423	0.5121381778785417
9	0.04886473471546781	0.3536012322844466
>10	0.21005725348393128	2.782759315142068
>50	0.011349436541021581	0.6377602407138974
>100	0.004413638460395106	0.7165046814058944
>500	4.203465199643809E-4	0.22627353026326005
>1k	4.203465199643809E-4	0.4550818648237187
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	1565	0.22048961374164533	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1128	0.15892158741250859	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	751	0.10580683700956912	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	7.044396605164388E-5	2.817758642065755E-4
18-19	0.0	0.0	0.0	3.522198302582194E-4	4.226637963098633E-4
20-21	0.0	0.0	0.0	8.453275926197266E-4	4.931077623615072E-4
22-23	0.0	0.0	0.0	0.0019724310494460286	5.63551728413151E-4
24-25	0.0	0.0	0.0	0.004297081929150277	5.63551728413151E-4
26-27	0.0	0.0	0.0	0.008241944028042333	5.63551728413151E-4
28-29	0.0	0.0	0.0	0.017188327716601108	5.63551728413151E-4
30-31	0.0	0.0	0.0	0.03374265973873742	7.044396605164388E-4
32-33	0.0	0.0	0.0	0.0633995694464795	8.453275926197266E-4
34-35	0.0	0.0	0.0	0.09770578091363005	9.862155247230143E-4
36-37	0.0	0.0	0.0	0.1470165571497808	9.862155247230143E-4
38-39	0.0	0.0	0.0	0.2146427645593589	0.00126799138892959
40-41	0.0	1.4088793210328775E-4	0.0	0.29706220483978224	0.0013384353549812339
42-43	0.0	2.817758642065755E-4	0.0	0.36884460624640736	0.0014088793210328776
44-45	0.0	2.817758642065755E-4	0.0	0.4403452317888259	0.0014088793210328776
46-47	0.0	2.817758642065755E-4	0.0	0.5308657281651883	0.0014088793210328776
48-49	0.0	2.817758642065755E-4	0.0	0.6295577246035413	0.0016906551852394532
50-51	0.0	2.817758642065755E-4	0.0	0.7196555571835939	0.0016906551852394532
52-53	0.0	2.817758642065755E-4	0.0	0.8038360966153083	0.001831543117342741
54-55	0.0	2.817758642065755E-4	0.0	0.8854806532691635	0.0021133189815493165
56-57	0.0	2.817758642065755E-4	0.0	0.9767055893060423	0.0021133189815493165
58-59	0.0	2.817758642065755E-4	0.0	1.0827237582137665	0.0021133189815493165
60-61	0.0	2.817758642065755E-4	0.0	1.1844448451923402	0.0021133189815493165
62-63	0.0	2.817758642065755E-4	0.0	1.2801781950565243	0.00253598277785918
64-65	0.0	2.817758642065755E-4	0.0	1.3722484586860229	0.00253598277785918
66-67	0.0	2.817758642065755E-4	0.0	1.4755897568837844	0.00253598277785918
68-69	0.0	2.817758642065755E-4	0.0	1.5937947319184427	0.00253598277785918
70-71	0.0	2.817758642065755E-4	0.0	1.7223549699626928	0.0026768707099624673
72-73	0.0	2.817758642065755E-4	0.0	1.8433072596733655	0.0028177586420657552
74-75	0.0	2.817758642065755E-4	0.0	1.9620757864364369	0.0028177586420657552
76-77	0.0	2.817758642065755E-4	0.0	2.08563450289102	0.0028177586420657552
78-79	0.0	2.817758642065755E-4	0.0	2.2280722022474446	0.0028177586420657552
80-81	0.0	2.817758642065755E-4	0.0	2.377272522344826	0.002888202608117399
82-83	0.0	2.817758642065755E-4	0.0	2.5314039200658227	0.0030995345062723306
84-85	0.0	2.817758642065755E-4	0.0	2.6776455935890358	0.0032404224383756185
86-87	0.0	2.817758642065755E-4	0.0	2.8305794438871543	0.0032404224383756185
88-89	0.0	2.817758642065755E-4	0.0	2.9902759149262312	0.0032404224383756185
90-91	0.0	2.817758642065755E-4	0.0	3.152085704946857	0.0032404224383756185
92-93	0.0	2.817758642065755E-4	0.0	3.3235463183165583	0.003522198302582194
94-95	0.0	2.817758642065755E-4	0.0	3.4971906946338605	0.003663086234685482
96-97	0.0	2.817758642065755E-4	0.0	3.6637202303799468	0.003663086234685482
98-99	0.0	2.817758642065755E-4	0.0	3.8570889171917093	0.003663086234685482
100-101	0.0	2.817758642065755E-4	0.0	4.075394767985753	0.003663086234685482
102-103	0.0	2.817758642065755E-4	0.0	4.296166157591605	0.003663086234685482
104-105	0.0	2.817758642065755E-4	0.0	4.519473529975317	0.0037335302007371254
106-107	0.0	2.817758642065755E-4	0.0	4.7407380273435304	0.0038039741667887693
108-109	0.0	2.817758642065755E-4	0.0	4.967990261826133	0.0038744181328404133
110-111	0.0	2.817758642065755E-4	0.0	5.214685030938989	0.003944862098892057
112-113	0.0	2.817758642065755E-4	0.0	5.477441024311622	0.003944862098892057
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3315	0.0	25.527033	1
GGTATCA	2535	0.0	24.448427	1
ATCAACG	3710	0.0	22.79791	3
AACGCAG	3800	0.0	22.558693	6
TCAACGC	3785	0.0	22.346169	4
GTTTAGA	245	4.275953E-7	21.891321	1
CAACGCA	3830	0.0	21.77258	5
TATCAAC	3915	0.0	21.759367	2
GTCTTAA	275	5.4173142E-8	21.670197	1
CATGGGG	2375	0.0	21.568275	4
GTACATG	5485	0.0	20.317043	1
ACGCAGA	4165	0.0	20.160095	7
CGCAGAG	4275	0.0	19.782055	8
TACATGG	5575	0.0	19.76828	2
ACATGGG	5475	0.0	19.582537	3
GCAGAGT	4510	0.0	18.757912	9
GTCTTAT	235	1.6718991E-4	17.751118	1
CATGGGT	975	0.0	17.71634	4
GTAGTAT	170	0.009600051	17.527367	1
AATAGAC	170	0.00963921	17.514978	7
>>END_MODULE