FastQCFastQC Report
Sun 1 Jan 2017
ERR1142536_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142536_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences709784
Sequences flagged as poor quality0
Sequence length125
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28100.3958950892102386No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23020.32432401970176844No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13870.19541156182726013No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA9280.13074400099185104No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG659.094947E-1173.213522
CTCAACG3400.045.5181431
GTATCAA43000.030.5924221
GGTATCA31500.030.4232351
CATGGGG22300.028.2758484
TTTACCG850.00721683727.9934023
ATCACGC1704.4297485E-727.9934023
TATACTC1608.751178E-626.0251165
ATCAACG51500.025.989023
CCGCAGA4000.025.2975831
TATCAAC53300.025.2229482
TCAACGC54200.024.9138724
CAACGCA54650.024.8175745
AACGCAG56050.024.6222086
ATGGGGG12600.023.6055475
ACGCAGA60200.022.6267917
GTATCAC2403.5453377E-722.3213961
GAGTACT25450.022.20185312-13
CGCAGAG62900.021.6540078
ACATGGG55350.021.6019553
Produced by FastQC (version 0.11.5)