##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142536_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	709784
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	22.05865305501392	14.0	14.0	33.0	14.0	33.0
2	27.604638876052434	27.0	27.0	33.0	14.0	33.0
3	31.173444315453715	33.0	33.0	33.0	27.0	33.0
4	30.804701148518422	33.0	33.0	33.0	27.0	33.0
5	32.287133832264466	33.0	33.0	33.0	33.0	33.0
6	34.94544255717232	37.0	37.0	37.0	33.0	37.0
7	35.09261690880606	37.0	37.0	37.0	33.0	37.0
8	35.43325856880403	37.0	37.0	37.0	33.0	37.0
9	35.89413539893827	37.0	37.0	37.0	33.0	37.0
10-11	36.00834056558051	37.0	37.0	37.0	33.0	37.0
12-13	36.08424182568218	37.0	37.0	37.0	37.0	37.0
14-15	36.08341551796039	37.0	37.0	37.0	37.0	37.0
16-17	36.08991608714764	37.0	37.0	37.0	37.0	37.0
18-19	36.08353034162506	37.0	37.0	37.0	37.0	37.0
20-21	36.102698849227366	37.0	37.0	37.0	37.0	37.0
22-23	36.10395275182309	37.0	37.0	37.0	37.0	37.0
24-25	36.09305084363694	37.0	37.0	37.0	37.0	37.0
26-27	36.05346274359523	37.0	37.0	37.0	37.0	37.0
28-29	36.03080091971642	37.0	37.0	37.0	37.0	37.0
30-31	35.97387021967246	37.0	37.0	37.0	35.0	37.0
32-33	35.93034007529051	37.0	37.0	37.0	33.0	37.0
34-35	35.87555228069384	37.0	37.0	37.0	33.0	37.0
36-37	35.81964442703696	37.0	37.0	37.0	33.0	37.0
38-39	35.77961605220743	37.0	37.0	37.0	33.0	37.0
40-41	35.658569367582245	37.0	37.0	37.0	33.0	37.0
42-43	35.621896943295425	37.0	37.0	37.0	33.0	37.0
44-45	35.560693112270776	37.0	37.0	37.0	33.0	37.0
46-47	35.4473198888676	37.0	37.0	37.0	33.0	37.0
48-49	35.336746108675314	37.0	37.0	37.0	33.0	37.0
50-51	35.2519393223854	37.0	37.0	37.0	33.0	37.0
52-53	35.168161017999836	37.0	37.0	37.0	33.0	37.0
54-55	35.107643311204534	37.0	37.0	37.0	33.0	37.0
56-57	35.055828956414906	37.0	37.0	37.0	33.0	37.0
58-59	35.006149758236305	37.0	37.0	37.0	30.0	37.0
60-61	34.96726187121716	37.0	37.0	37.0	27.0	37.0
62-63	34.9547721560362	37.0	37.0	37.0	27.0	37.0
64-65	34.912257813644715	37.0	37.0	37.0	27.0	37.0
66-67	34.868147915422156	37.0	37.0	37.0	27.0	37.0
68-69	34.818456741769324	37.0	37.0	37.0	27.0	37.0
70-71	34.78195408744068	37.0	37.0	37.0	27.0	37.0
72-73	34.744158081895335	37.0	37.0	37.0	27.0	37.0
74-75	34.63673314698556	37.0	37.0	37.0	27.0	37.0
76-77	34.58338029597736	37.0	37.0	37.0	27.0	37.0
78-79	34.55177631504796	37.0	37.0	37.0	27.0	37.0
80-81	34.51493834744091	37.0	37.0	37.0	27.0	37.0
82-83	34.47081083822684	37.0	37.0	37.0	27.0	37.0
84-85	34.398128867373735	37.0	37.0	37.0	27.0	37.0
86-87	34.215803681119894	37.0	37.0	37.0	27.0	37.0
88-89	34.139338869289816	37.0	37.0	37.0	27.0	37.0
90-91	34.088343777825365	37.0	37.0	37.0	27.0	37.0
92-93	34.015240552055275	37.0	37.0	37.0	27.0	37.0
94-95	34.024732876480726	37.0	37.0	37.0	27.0	37.0
96-97	33.96783880729912	37.0	37.0	37.0	27.0	37.0
98-99	33.89856702884258	37.0	33.0	37.0	22.0	37.0
100-101	33.84300012398138	37.0	33.0	37.0	22.0	37.0
102-103	33.73308217711303	37.0	33.0	37.0	22.0	37.0
104-105	33.62391657180213	37.0	33.0	37.0	22.0	37.0
106-107	33.548346398340904	37.0	33.0	37.0	22.0	37.0
108-109	33.43452022023601	37.0	33.0	37.0	22.0	37.0
110-111	33.357513694307	37.0	33.0	37.0	22.0	37.0
112-113	33.296044289530336	37.0	33.0	37.0	22.0	37.0
114-115	33.15940835521793	37.0	33.0	37.0	14.0	37.0
116-117	33.02874043370941	37.0	33.0	37.0	14.0	37.0
118-119	32.872837370242216	37.0	33.0	37.0	14.0	37.0
120-121	32.78835321731682	37.0	33.0	37.0	14.0	37.0
122-123	32.63853369475784	37.0	33.0	37.0	14.0	37.0
124-125	31.27993727669263	35.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	15.0
16	5.0
17	21.0
18	86.0
19	354.0
20	1408.0
21	3432.0
22	5304.0
23	7202.0
24	6076.0
25	5239.0
26	5493.0
27	6789.0
28	8801.0
29	11520.0
30	15759.0
31	21531.0
32	30515.0
33	46394.0
34	76543.0
35	156026.0
36	301271.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	44.38982592225128	12.674613285830253	12.125264949941371	30.81029584197709
2	17.85994048893748	20.111752307744325	37.19032832523697	24.83797887808122
3	20.636539748511854	25.28054665210806	28.133140784051285	25.949772815328803
4	14.717012499577336	17.784847221126427	33.52259278879208	33.975547490504155
5	16.948283319549546	34.525501887228415	31.91282822206035	16.613386571161683
6	31.418556224622346	33.733082738546415	18.32502557137584	16.523335465455396
7	27.24093523416079	30.929682722628172	22.310159131833977	19.519222911377064
8	26.66585890918927	31.142713839703344	21.090923435862177	21.1005038152452
9	27.09796219695006	15.528245212628066	20.33477790426383	37.03901468615805
10-11	25.840255627064007	24.53077274213	26.691007405069712	22.93796422573628
12-13	26.912765010200285	23.31209212943656	26.443115088533975	23.332027771829175
14-15	25.023457840695194	23.811046740980355	24.853194774748374	26.312300643576076
16-17	24.289713490301274	25.91619422246768	25.652874677366633	24.14121760986441
18-19	24.14128805383046	24.63953822571374	27.865026543286408	23.354147177169395
20-21	25.126694008433585	23.850384837013106	27.629085591189394	23.39383556336391
22-23	24.943433495260532	23.645785196623198	27.550846454696078	23.859934853420196
24-25	24.230681446749998	24.36537030984074	27.47948671708576	23.9244615263235
26-27	24.075775165402433	24.398056310088702	27.631363907893103	23.894804616615758
28-29	23.938338987635674	24.340574033790563	27.9011642978709	23.819922680702863
30-31	24.246390451179515	24.15586995480315	27.933709410186765	23.664030183830572
32-33	23.88064537943938	24.196234347350742	27.67665937806431	24.246460895145567
34-35	24.13260400193017	24.290187487011867	27.752727067798936	23.824481443259028
36-37	24.113479673731497	24.404624790517108	27.434985456832965	24.046910078918433
38-39	24.23152677434262	24.25611171849464	27.370580345569923	24.141781161592824
40-41	23.96082680188399	24.485714406477584	27.599606070126843	23.953852721511588
42-43	24.219461595629646	24.36302670184176	27.443688735633803	23.97382296689479
44-45	24.126565264925667	24.437011823315263	27.1598119991434	24.276610912615666
46-47	24.095407771853186	24.36035891334104	27.217759405201946	24.326473909603827
48-49	24.252980855598018	24.70244280455802	26.904454111555324	24.140122228288625
50-51	24.019138216696913	24.897504029394856	26.701855775841665	24.381501978066566
52-53	24.199686101687274	24.622702117827394	26.652051891843154	24.525559888642178
54-55	24.218987748385423	24.62629476009603	26.721368754437968	24.433348737080575
56-57	24.35762147357506	24.513091306651038	26.379645075121445	24.74964214465246
58-59	24.216803985437824	24.551905931945495	26.622888089897774	24.60840199271891
60-61	24.117830213135264	24.6250267687071	26.93481397157445	24.322329046583185
62-63	23.69354620560621	24.782680364730677	26.899521544582576	24.624251885080533
64-65	23.851833797324257	24.386644387588337	26.973558152902854	24.787963662184552
66-67	24.0132978648459	24.536589558400422	26.91993383473902	24.53017874201466
68-69	24.201376756872513	24.416230853330028	26.802872423159723	24.57951996663774
70-71	23.794421964992164	24.44166112507467	27.15938933534709	24.604527574586072
72-73	23.95876773779065	24.43827981470419	27.136847266210566	24.46610518129459
74-75	24.06494127200978	24.409089691478908	27.20795672273706	24.318012313774254
76-77	23.95968689838291	24.35994283668712	27.14658387194065	24.53378639298932
78-79	23.988441545908014	24.39666461674581	27.198082232117258	24.41681160522892
80-81	23.67382189511175	24.543029992222987	27.448702703921192	24.334445408744067
82-83	23.79406974516191	24.582055949415597	27.23730036180021	24.386573943622285
84-85	23.676156939806283	24.651286381455694	26.939792188533012	24.732764490205007
86-87	23.73014982344027	24.54762524871171	27.013038528346605	24.709186399501412
88-89	23.722982780140402	24.31337998339574	27.0645900046876	24.89904723177626
90-91	24.01369829194573	24.411315550947137	26.858033639111373	24.71695251799576
92-93	23.727501205859205	24.69310530606258	26.82105895593748	24.758334532140733
94-95	23.460674980469395	24.62455100745509	26.958009026696168	24.956764985379344
96-97	23.68410671415529	24.521474138611183	26.796673354147178	24.99774579308635
98-99	23.504333712791496	24.65031615251964	26.603797775097775	25.241552359591086
100-101	23.733910598153805	24.82980737801923	26.386407695862403	25.049874327964567
102-103	23.855215107694736	24.639186005883477	26.639653753818067	24.865945132603724
104-105	23.80111401735906	24.73394681542774	26.400887043542763	25.06405212367044
106-107	23.693275718506392	24.76517073816766	26.513410663439334	25.028142879886612
108-109	23.758762577217404	24.84693888333541	26.243386019748776	25.15091251969841
110-111	23.644932232522752	24.897996562315083	26.310476485671618	25.146594719490544
112-113	23.669383925250497	24.96477801697418	26.19092569006909	25.17491236770623
114-115	23.558169498163878	25.0003698310823	26.278294719446144	25.163165951307686
116-117	23.59576998072653	25.10150975508042	26.136613392243273	25.16610687194978
118-119	23.38619918172289	24.86967866280446	26.361963639642482	25.382158515830167
120-121	23.29362138584177	24.847276190583536	26.48835269619515	25.37074972737955
122-123	23.42769067772731	25.015004564769	26.29236500118346	25.26493975632023
124-125	23.367531530719205	24.9121563743336	26.37647509664912	25.343836998298073
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	150.0
1	145.5
2	324.0
3	490.0
4	371.5
5	314.5
6	439.5
7	847.5
8	1412.5
9	1649.5
10	1459.0
11	1171.0
12	1049.0
13	1032.5
14	1022.0
15	975.0
16	941.5
17	965.5
18	956.5
19	895.0
20	881.0
21	906.5
22	936.0
23	993.0
24	1096.5
25	1241.5
26	1473.0
27	1733.5
28	2101.0
29	2700.5
30	3495.0
31	4348.0
32	5439.0
33	6614.5
34	7631.0
35	8708.0
36	9935.0
37	11333.5
38	12539.5
39	13469.0
40	14339.0
41	15098.5
42	15952.0
43	17580.0
44	19685.5
45	21591.5
46	23782.0
47	25769.5
48	28029.0
49	30269.5
50	31641.0
51	31304.0
52	30376.5
53	30443.5
54	30142.5
55	29271.5
56	27905.0
57	25771.5
58	24066.5
59	22058.0
60	19379.0
61	16432.0
62	13244.5
63	10318.5
64	7915.5
65	6377.0
66	5052.0
67	3814.0
68	2952.5
69	2520.5
70	2203.0
71	1928.0
72	1665.5
73	1643.0
74	1345.5
75	1074.0
76	892.0
77	597.0
78	413.0
79	271.0
80	181.0
81	145.0
82	105.0
83	55.0
84	32.0
85	21.0
86	14.0
87	4.5
88	1.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.030431793334310155
2	0.0
3	0.0012679913889295897
4	0.0
5	0.001831543117342741
6	8.453275926197266E-4
7	0.00817150006199069
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	4.226637963098633E-4
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	2.1133189815493165E-4
36-37	7.044396605164388E-5
38-39	0.0
40-41	0.001831543117342741
42-43	2.1133189815493165E-4
44-45	0.0
46-47	0.004297081929150277
48-49	0.006551288842802881
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.006480844876751237
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.07002130225533401
76-77	0.033531327840582485
78-79	7.044396605164388E-5
80-81	0.0
82-83	0.0
84-85	0.05508718145238552
86-87	0.1949184540648986
88-89	0.21584031198223683
90-91	0.131941548414729
92-93	0.10510239734905268
94-95	7.044396605164388E-5
96-97	0.0
98-99	0.0
100-101	0.0
102-103	0.0
104-105	0.0014793232870845216
106-107	0.001831543117342741
108-109	0.002606426743910824
110-111	5.63551728413151E-4
112-113	0.0
114-115	7.044396605164388E-5
116-117	0.0
118-119	0.0
120-121	8.453275926197266E-4
122-123	0.0
124-125	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	709784.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.68953636202724
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.20573135529341	68.6217856845954
2	9.145117491458363	14.392501107582484
3	1.9395730339485189	4.578723083450983
4	0.6858255647523414	2.158691828623489
5	0.33089187635588	1.3018864168202722
6	0.196923518509324	0.9297492222166677
7	0.11476062551196736	0.6321322289907063
8	0.0736267593209787	0.4634924443845104
9	0.05430752391903484	0.38460904903426996
>10	0.2307194474931603	3.0374641035511187
>50	0.013173743942349443	0.7368465229933542
>100	0.007861709120622716	1.2085103588900816
>500	8.499144994711448E-4	0.46698525626180365
>1k	6.374358746033586E-4	1.0866226926048914
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2810	0.3958950892102386	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2302	0.32432401970176844	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1387	0.19541156182726013	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	928	0.13074400099185104	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.4088793210328775E-4
2	0.0	0.0	0.0	0.0	1.4088793210328775E-4
3	0.0	0.0	0.0	0.0	1.4088793210328775E-4
4	0.0	0.0	0.0	0.0	1.4088793210328775E-4
5	0.0	0.0	0.0	0.0	1.4088793210328775E-4
6	0.0	0.0	0.0	0.0	1.4088793210328775E-4
7	0.0	0.0	0.0	0.0	1.4088793210328775E-4
8	0.0	0.0	0.0	0.0	1.4088793210328775E-4
9	0.0	0.0	0.0	0.0	1.4088793210328775E-4
10-11	0.0	0.0	0.0	0.0	1.4088793210328775E-4
12-13	0.0	0.0	0.0	0.0	4.226637963098633E-4
14-15	0.0	0.0	0.0	0.0	6.33995694464795E-4
16-17	0.0	0.0	0.0	2.1133189815493163E-4	7.044396605164388E-4
18-19	0.0	0.0	0.0	4.931077623615072E-4	8.453275926197266E-4
20-21	0.0	0.0	0.0	9.862155247230143E-4	0.00126799138892959
22-23	0.0	0.0	0.0	0.0021133189815493165	0.00126799138892959
24-25	0.0	0.0	0.0	0.004437969861253565	0.00126799138892959
26-27	0.0	0.0	0.0	0.00852371989224891	0.0013384353549812339
28-29	0.0	0.0	0.0	0.017540547546859325	0.0014088793210328776
30-31	0.0	0.0	0.0	0.03388354767084071	0.0014793232870845214
32-33	0.0	0.0	0.0	0.06375178927673772	0.0016906551852394532
34-35	0.0	0.0	0.0	0.097917112811785	0.0016906551852394532
36-37	0.0	0.0	0.0	0.1472278890479357	0.0017610991512910972
38-39	0.0	0.0	0.0	0.2141496567969974	0.0020428750154976726
40-41	0.0	0.0	0.0	0.2964986531113691	0.0021133189815493165
42-43	0.0	0.0	0.0	0.3685628303822008	0.0021133189815493165
44-45	0.0	0.0	0.0	0.440133899890671	0.0021133189815493165
46-47	0.0	0.0	0.0	0.5311475040293949	0.00218376294760096
48-49	0.0	1.4088793210328775E-4	0.0	0.6304030521961611	0.002254206913652604
50-51	0.0	1.4088793210328775E-4	0.0	0.7209939925385751	0.002254206913652604
52-53	0.0	1.4088793210328775E-4	0.0	0.8049632000721346	0.002395094845755892
54-55	0.0	1.4088793210328775E-4	0.0	0.886396424827835	0.00253598277785918
56-57	0.0	1.4088793210328775E-4	0.0	0.9780440246610236	0.0027473146760141117
58-59	0.0	1.4088793210328775E-4	0.0	1.0838508616705926	0.002958646574169043
60-61	0.0	1.4088793210328775E-4	0.0	1.1844448451923402	0.002958646574169043
62-63	0.0	1.4088793210328775E-4	0.0	1.280037307124421	0.0032404224383756185
64-65	0.0	1.4088793210328775E-4	0.0	1.3725302345502293	0.0032404224383756185
66-67	0.0	1.4088793210328775E-4	0.0	1.476435084476404	0.0032404224383756185
68-69	0.0	1.4088793210328775E-4	0.0	1.5954853871036823	0.0033108664044272625
70-71	0.0	1.4088793210328775E-4	0.0	1.7240456251479324	0.003522198302582194
72-73	0.0	1.4088793210328775E-4	0.0	1.84394125536783	0.0038039741667887693
74-75	0.0	1.4088793210328775E-4	0.0	1.9629211140290566	0.0038039741667887693
76-77	0.0	1.4088793210328775E-4	0.0	2.087466046008363	0.0038039741667887693
78-79	0.0	1.4088793210328775E-4	0.0	2.229340193636374	0.0038039741667887693
80-81	0.0	2.817758642065755E-4	0.0	2.3780474059713943	0.0038039741667887693
82-83	0.0	4.226637963098633E-4	0.0	2.531967471794236	0.0038039741667887693
84-85	0.0	4.226637963098633E-4	0.0	2.6790544729100683	0.0038744181328404133
86-87	0.0	4.226637963098633E-4	0.0	2.832692762868704	0.004015306064943701
88-89	0.0	4.226637963098633E-4	0.0	2.992741453738039	0.004085750030995345
90-91	0.0	4.226637963098633E-4	0.0	3.154692131690768	0.004085750030995345
92-93	0.0	4.226637963098633E-4	0.0	3.3255187493660046	0.004367525895201921
94-95	0.0	4.226637963098633E-4	0.0	3.4990926817172547	0.004367525895201921
96-97	0.0	4.226637963098633E-4	0.0	3.667805980410942	0.004719745725460141
98-99	0.0	4.226637963098633E-4	0.0	3.8625835465437373	0.005142409521770003
100-101	0.0	4.226637963098633E-4	0.0	4.081875612862504	0.005212853487821647
102-103	0.0	4.226637963098633E-4	0.0	4.3027878904004595	0.005283297453873291
104-105	0.0	4.226637963098633E-4	0.0	4.526940590376791	0.005353741419924935
106-107	0.0	4.226637963098633E-4	0.0	4.750529738624708	0.005353741419924935
108-109	0.0	4.226637963098633E-4	0.0	4.97855685673388	0.005353741419924935
110-111	0.0	4.226637963098633E-4	0.0	5.226167397405408	0.0054946293520282226
112-113	0.0	4.226637963098633E-4	0.0	5.488359839049627	0.0054946293520282226
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCACG	65	9.094947E-11	73.21352	2
CTCAACG	340	0.0	45.518143	1
GTATCAA	4300	0.0	30.592422	1
GGTATCA	3150	0.0	30.423235	1
CATGGGG	2230	0.0	28.275848	4
TTTACCG	85	0.007216837	27.993402	3
ATCACGC	170	4.4297485E-7	27.993402	3
TATACTC	160	8.751178E-6	26.025116	5
ATCAACG	5150	0.0	25.98902	3
CCGCAGA	400	0.0	25.297583	1
TATCAAC	5330	0.0	25.222948	2
TCAACGC	5420	0.0	24.913872	4
CAACGCA	5465	0.0	24.817574	5
AACGCAG	5605	0.0	24.622208	6
ATGGGGG	1260	0.0	23.605547	5
ACGCAGA	6020	0.0	22.626791	7
GTATCAC	240	3.5453377E-7	22.321396	1
GAGTACT	2545	0.0	22.201853	12-13
CGCAGAG	6290	0.0	21.654007	8
ACATGGG	5535	0.0	21.601955	3
>>END_MODULE