FastQCFastQC Report
Sun 1 Jan 2017
ERR1142534_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142534_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1016568
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22610.22241502781909328No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18570.18267346601506246No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30500.037.121471
GGTATCA20200.036.5798381
CAACGCA36200.029.9446935
TCAACGC36200.029.615634
AACGCAG37250.029.3942816
ATCAACG36350.029.1657143
TATCAAC39100.028.485372
ACGCAGA41800.025.4979447
CGCAGAG43950.023.9820168
GTACATG59250.023.6347481
TACATGG60200.023.250292
GCAGAGT47950.022.118729
ACATGGG62350.021.9709283
CATGGGT12000.019.853444
CATGGGG33650.019.646944
AGAGTAC44300.019.36811310-11
CAGAGTA46800.019.2883610-11
ATGGGGG17450.018.7725945
TGAACCG2251.2040302E-418.5298775
GAGTACA48150.017.88134212-13
Produced by FastQC (version 0.11.5)