FastQCFastQC Report
Sun 1 Jan 2017
ERR1142534_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142534_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1016568
Sequences flagged as poor quality0
Sequence length125
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36510.3591496092735557No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31830.31311235451047054No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19430.19113330342879178No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27400.049.4994431
CTCAACG4500.042.3012771
GTATCAA42100.039.8458441
ATCAACG52950.032.013333
CAACGCA54850.031.2281635
TCAACGC54750.031.1765734
TATCAAC55050.031.00822
AACGCAG57000.030.2589446
ACGCAGA62450.027.2373097
CGCAGAG66250.025.6750188
GCAGAGT71250.023.8732649
GAGTACT32750.022.15537612-13
CAGAGTA69850.022.09538510-11
CCGCAGA6300.021.7171751
ACATGGG56900.020.9059413
AGAGTAC68000.020.81606510-11
GTACATG57150.020.6093851
TACATGG58950.020.2798252
AGTACTT34200.020.08567612-13
CGTATCA1500.00471186219.8287241
Produced by FastQC (version 0.11.5)