FastQCFastQC Report
Sun 1 Jan 2017
ERR1142533_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142533_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences469901
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10130.21557732373414826No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA6990.14875473770006875No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6770.1440729004620122No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT5890.1253455515097861No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGCG453.1564108E-452.9314963
ATTAACG350.007768614751.041083
TTAACGC700.002776324534.0273864
GACCGGA850.007159095728.0375351
GTACATA2057.6488504E-826.156971
GTATGAT1200.001276713824.8249021
GTTAAGC1300.002034445322.9152931
CTATCCA1653.1939102E-421.6537934
GCTTAGT1450.003834131220.5447441
CATGGGG17050.019.9075164
GGTATCA30600.019.8599191
GCTATCC1500.004679956519.8493083
TACATAG1908.26082E-418.806622
ACATTAC1600.00677417518.6186751
TAAGAGA2652.0652751E-517.9767324
GTATAAG3002.982546E-617.8739281
GTATCAA36150.017.4701371
ATACTAT4602.4265319E-916.8377591
AATCCTT3558.0538666E-716.7633156
ATGGGGC4753.741661E-916.2973275
Produced by FastQC (version 0.11.5)