FastQCFastQC Report
Sun 1 Jan 2017
ERR1142533_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142533_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences469901
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21360.45456383365857916No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19970.42498313474540383No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10550.22451537664316526No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA8830.18791192187290515No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT6910.14705225143168454No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5410.11513063389948096No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG4970.1057669594233679No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGAT650.001941030336.6027157
GGTATCA30150.028.0224631
GTATCAA39100.026.4775491
CTCAACG2809.458745E-1125.4993151
CCGCAGA4450.025.4038141
GAGTACT20200.024.87594212-13
GTGTTAT1552.104071E-423.0316411
ATCAACG45600.022.8289573
CAACGCA46200.022.789995
TATCAAC46450.022.6673322
AACGCAG47350.022.4853526
TCAACGC47700.022.0733224
GTACTTT23950.021.60169814-15
ACGCAGA49900.021.33637
CGCAGAG52000.020.6990018
ATCGTAC750.00471760819.82435842-43
GCAGAGT54750.019.5507139
AGTACTT20700.019.39133312-13
CATGGGG16300.018.9770434
GTCTTAT1908.313191E-418.7889691
Produced by FastQC (version 0.11.5)