##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142533_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	469901
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	22.778968335883516	27.0	14.0	33.0	14.0	33.0
2	28.075243508739074	33.0	27.0	33.0	14.0	33.0
3	31.29295106841654	33.0	33.0	33.0	27.0	33.0
4	30.97496919563908	33.0	33.0	33.0	27.0	33.0
5	32.29332135917991	33.0	33.0	33.0	33.0	33.0
6	35.15881643154622	37.0	37.0	37.0	33.0	37.0
7	35.32308720347478	37.0	37.0	37.0	33.0	37.0
8	35.604025103160026	37.0	37.0	37.0	33.0	37.0
9	35.98033202738449	37.0	37.0	37.0	33.0	37.0
10-11	36.081849155460404	37.0	37.0	37.0	37.0	37.0
12-13	36.144429358524455	37.0	37.0	37.0	37.0	37.0
14-15	36.135806265575084	37.0	37.0	37.0	37.0	37.0
16-17	36.13853556387409	37.0	37.0	37.0	37.0	37.0
18-19	36.138266358232904	37.0	37.0	37.0	37.0	37.0
20-21	36.15296200689082	37.0	37.0	37.0	37.0	37.0
22-23	36.147493833807545	37.0	37.0	37.0	37.0	37.0
24-25	36.12921870777036	37.0	37.0	37.0	37.0	37.0
26-27	36.0958904109589	37.0	37.0	37.0	37.0	37.0
28-29	36.06327077405666	37.0	37.0	37.0	37.0	37.0
30-31	35.99000321344283	37.0	37.0	37.0	37.0	37.0
32-33	35.941380205617776	37.0	37.0	37.0	35.0	37.0
34-35	35.86720287890428	37.0	37.0	37.0	33.0	37.0
36-37	35.79901404763982	37.0	37.0	37.0	33.0	37.0
38-39	35.74673282244558	37.0	37.0	37.0	33.0	37.0
40-41	35.618294066196924	37.0	37.0	37.0	33.0	37.0
42-43	35.57209178103473	37.0	37.0	37.0	33.0	37.0
44-45	35.4919866099455	37.0	37.0	37.0	33.0	37.0
46-47	35.34740615576472	37.0	37.0	37.0	33.0	37.0
48-49	35.21633918633925	37.0	37.0	37.0	33.0	37.0
50-51	35.11969542520659	37.0	37.0	37.0	33.0	37.0
52-53	35.04185668896214	37.0	37.0	37.0	33.0	37.0
54-55	34.98011496038527	37.0	37.0	37.0	30.0	37.0
56-57	34.92682714018485	37.0	37.0	37.0	27.0	37.0
58-59	34.88130372142217	37.0	37.0	37.0	27.0	37.0
60-61	34.850541922660305	37.0	37.0	37.0	27.0	37.0
62-63	34.83615484963855	37.0	37.0	37.0	27.0	37.0
64-65	34.7992343068008	37.0	37.0	37.0	27.0	37.0
66-67	34.758824731166776	37.0	37.0	37.0	27.0	37.0
68-69	34.7263349088425	37.0	37.0	37.0	27.0	37.0
70-71	34.688396066405474	37.0	37.0	37.0	27.0	37.0
72-73	34.66705859319303	37.0	37.0	37.0	27.0	37.0
74-75	34.55932845429143	37.0	37.0	37.0	27.0	37.0
76-77	34.50140242306358	37.0	37.0	37.0	27.0	37.0
78-79	34.47247824541765	37.0	37.0	37.0	27.0	37.0
80-81	34.446767510603294	37.0	37.0	37.0	27.0	37.0
82-83	34.40511937620903	37.0	37.0	37.0	27.0	37.0
84-85	34.30956839844988	37.0	37.0	37.0	27.0	37.0
86-87	34.13979008344311	37.0	37.0	37.0	27.0	37.0
88-89	34.05981366287793	37.0	37.0	37.0	27.0	37.0
90-91	34.01791228365124	37.0	37.0	37.0	27.0	37.0
92-93	33.958224179135605	37.0	37.0	37.0	27.0	37.0
94-95	33.96703667368233	37.0	37.0	37.0	27.0	37.0
96-97	33.92308699066399	37.0	37.0	37.0	22.0	37.0
98-99	33.86107924860768	37.0	37.0	37.0	22.0	37.0
100-101	33.7997205794412	37.0	37.0	37.0	22.0	37.0
102-103	33.700042136535146	37.0	33.0	37.0	22.0	37.0
104-105	33.61158839840732	37.0	33.0	37.0	22.0	37.0
106-107	33.539592382225194	37.0	33.0	37.0	22.0	37.0
108-109	33.43487990023431	37.0	33.0	37.0	22.0	37.0
110-111	33.34988220923131	37.0	33.0	37.0	14.0	37.0
112-113	33.29534412567753	37.0	33.0	37.0	14.0	37.0
114-115	33.17472084545469	37.0	33.0	37.0	14.0	37.0
116-117	33.05135124207014	37.0	33.0	37.0	14.0	37.0
118-119	32.90231240197403	37.0	33.0	37.0	14.0	37.0
120-121	32.821242133981414	37.0	33.0	37.0	14.0	37.0
122-123	32.69029646670256	37.0	33.0	37.0	14.0	37.0
124-125	31.383660600849968	37.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	6.0
16	3.0
17	9.0
18	63.0
19	306.0
20	1186.0
21	3045.0
22	4617.0
23	6262.0
24	5147.0
25	3636.0
26	3613.0
27	4400.0
28	5527.0
29	7216.0
30	9487.0
31	13184.0
32	18361.0
33	27510.0
34	45077.0
35	93408.0
36	217838.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	44.44217387324678	14.01169486077496	12.480549364701274	29.065581901276982
2	17.76650826450678	20.529217856527225	37.5157745993305	24.188499279635497
3	20.641288109334365	25.964996658849365	28.385734776483407	25.007980455332863
4	14.786731673267347	18.42451920723727	33.78264783433106	33.00610128516433
5	16.739379338818352	34.2727810575331	32.3174131597877	16.67042644386085
6	30.265420433457617	33.860471253213476	18.72712259734069	17.14698571598822
7	26.350032669375278	31.409635656654444	22.87877852717517	19.361553146795103
8	26.059531688589725	31.36086111755455	21.351093102589694	21.22851409126603
9	26.637951398273252	16.040612809932306	20.961010936346167	36.36042485544827
10-11	25.414182987480345	25.02378160506149	26.660083719762245	22.901951687695917
12-13	26.805646295709096	23.899076614010184	26.596772511656713	22.698504578624007
14-15	24.853958599790168	24.018676274364175	25.48994362642344	25.63742149942222
16-17	23.674135615799923	26.201157265040937	26.38438734967578	23.74031976948336
18-19	23.47217818221285	24.80150074164558	28.782020042519598	22.944301033621976
20-21	24.720153224090232	23.908597573951905	28.641200255373484	22.73004894658438
22-23	24.466642973732768	23.979199874016015	28.32617934415973	23.22797780809149
24-25	23.786074087946186	24.561130961628088	28.339160801956158	23.31363414846957
26-27	23.545597902536915	24.516227886299454	28.515261725342146	23.42291248582148
28-29	23.27064637019287	24.494840402552878	28.98376466532312	23.250748561931132
30-31	23.764367388024286	24.252555325483453	28.962377181576542	23.02070010491572
32-33	23.367049655140125	24.36204647361891	28.65933462580416	23.611569245436804
34-35	23.359945350132634	24.393781236666058	28.72671980557586	23.519553607625443
36-37	23.468184720153225	24.554373270908705	28.33624175356459	23.641200255373484
38-39	23.518783743809866	24.329486423736064	28.424817142334234	23.726912690119832
40-41	23.26213650085285	24.51592857545683	28.698356758804373	23.523578164885947
42-43	23.70586964687205	24.33387493735376	28.400161310745574	23.560094105028618
44-45	23.63316953996693	24.455364002204718	28.097194941062053	23.814271516766297
46-47	23.49107911479621	24.54975254346584	28.05766572564727	23.901502616090685
48-49	23.75055068774781	24.688262848848716	27.832024807019856	23.729161656383617
50-51	23.428871187760826	25.002926148273787	27.55867725329378	24.00952541067161
52-53	23.4695180474185	24.885880217322374	27.222329809896127	24.422271925363003
54-55	23.670517832479607	24.824377900876993	27.17519222134024	24.32991204530316
56-57	23.763835361065418	24.73659345266343	26.918542416381325	24.58102876988983
58-59	23.554773829778856	24.734704474670703	27.354195196642696	24.356326498907748
60-61	23.651790483527382	24.919397915731185	27.44046086303285	23.98835073770858
62-63	23.144662386332442	24.946424885241786	27.55239933517911	24.35651339324666
64-65	23.12178522710103	24.784369473569964	27.532501526917375	24.561343772411636
66-67	23.43926080974236	24.64002996602219	27.43008371544623	24.49062550878922
68-69	23.474838317007197	24.703394970429944	27.516221501975945	24.30554521058691
70-71	23.146258467209048	24.662216083813398	27.830862245451705	24.36066320352585
72-73	23.46057999450948	24.502288779977057	27.943545555340382	24.093585670173077
74-75	23.52206441390502	24.663139938282253	27.939525978741663	23.875269669071063
76-77	23.32685767206187	24.590197090742645	28.002959288493372	24.079985948702117
78-79	23.35108182822266	24.74819056861155	27.84045082028266	24.06027678288313
80-81	23.012081268182023	24.88736989280721	28.15284496095986	23.94770387805091
82-83	23.24330018450695	24.840870736601964	27.987490982143047	23.92833809674804
84-85	23.261154715920462	24.81346700112005	27.661502229452623	24.263876053506863
86-87	23.11520599730207	24.764997254080225	27.80598343917121	24.3138133094465
88-89	23.372704377962027	24.548822335361088	27.71173269930058	24.366740587376306
90-91	23.427606270180412	24.654894981937534	27.558529853688686	24.35896889419337
92-93	23.111487033517697	24.91424566008471	27.635161622962134	24.33910568343546
94-95	22.66948002821874	24.870052277024605	27.824188312206523	24.636279382550136
96-97	23.126999091297954	24.581880013024023	27.47355293987457	24.817567955803458
98-99	23.01154924122315	24.91024705203862	27.221372161370162	24.856831545368067
100-101	23.075498881679334	25.168067316307052	26.961530194658025	24.794903607355593
102-103	23.149557034354046	25.059640222089335	27.214668621688396	24.576134121868222
104-105	23.256373205191778	24.99505210716391	27.061994437078763	24.686580250565548
106-107	23.06660154525034	25.116755676576403	27.023366032371303	24.793276745801958
108-109	23.08564034735826	25.282327015181227	26.683081873504307	24.94895076395621
110-111	22.993771001853588	25.483135737679802	26.69445987329192	24.82863338717469
112-113	22.94227933117827	25.439826686897877	26.541548113325998	25.076345868597855
114-115	22.881546199674187	25.549025804399015	26.648194670999498	24.921233324927297
116-117	23.223107870708798	25.486991394986813	26.429850574749338	24.860050159555055
118-119	22.775861298443715	25.36183153472753	26.744356790047263	25.117950376781494
120-121	22.788054841747403	25.342654515080415	26.931511659457648	24.937778983714534
122-123	22.71606146826672	25.55197796982769	26.948335926078048	24.783624635827547
124-125	22.660943475327784	25.409501150242285	26.97100027452591	24.95855509990402
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	89.0
1	85.0
2	165.0
3	269.0
4	243.5
5	226.0
6	303.0
7	532.5
8	860.5
9	1041.0
10	977.0
11	883.5
12	854.5
13	842.0
14	837.0
15	848.0
16	909.0
17	898.0
18	860.5
19	834.0
20	848.5
21	883.5
22	902.0
23	938.5
24	1003.5
25	1108.5
26	1270.0
27	1517.0
28	1806.0
29	2186.5
30	2853.5
31	3488.5
32	4171.5
33	4978.0
34	5689.5
35	6538.5
36	7347.5
37	8297.5
38	9221.5
39	9601.5
40	9803.0
41	10292.0
42	10971.0
43	12046.0
44	13436.0
45	14815.0
46	16492.0
47	17733.0
48	19149.0
49	20701.0
50	21818.0
51	21571.0
52	19977.0
53	19235.0
54	19009.5
55	18177.5
56	16968.5
57	15406.0
58	14078.0
59	12822.0
60	11320.0
61	9465.5
62	7609.0
63	6098.0
64	4620.0
65	3627.0
66	2828.0
67	2239.0
68	1804.0
69	1528.5
70	1349.0
71	1128.5
72	965.5
73	941.0
74	727.0
75	553.5
76	460.5
77	314.5
78	231.0
79	153.0
80	106.5
81	76.5
82	43.0
83	19.5
84	10.0
85	9.0
86	6.5
87	1.5
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02596291559285892
2	0.0
3	6.384323506440718E-4
4	0.0
5	0.003192161753220359
6	0.0010640539177401197
7	0.008938052909017006
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	2.1281078354802395E-4
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	1.0640539177401198E-4
36-37	2.1281078354802395E-4
38-39	0.0
40-41	0.0013832700930621557
42-43	1.0640539177401198E-4
44-45	0.0
46-47	0.004362621062734491
48-49	0.007235566640632815
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	1.0640539177401198E-4
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.007554782815954851
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.07320690954052024
76-77	0.041178886616542634
78-79	4.256215670960479E-4
80-81	0.0
82-83	0.0
84-85	0.059374208609898685
86-87	0.2178118369614025
88-89	0.24590286038974168
90-91	0.1481163053494247
92-93	0.11427939076528887
94-95	1.0640539177401198E-4
96-97	0.0
98-99	0.0
100-101	0.0
102-103	0.0
104-105	8.512431341920958E-4
106-107	0.0020217024437062274
108-109	0.002447324010802275
110-111	4.256215670960479E-4
112-113	0.0
114-115	1.0640539177401198E-4
116-117	1.0640539177401198E-4
118-119	0.0
120-121	7.448377424180839E-4
122-123	0.0
124-125	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	469901.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.96219478594563
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.88322088358937	64.26073706622375
2	9.217967637027616	13.63562235800759
3	1.91043815418552	4.239005966591155
4	0.6657942696942419	1.9697442184996778
5	0.32957821048778296	1.2188163900650395
6	0.2012631217820511	0.8931517329882936
7	0.12713997942036304	0.6582486346079007
8	0.09363356262575676	0.5540275037942607
9	0.07071712290898426	0.4707354257366358
>10	0.4465243022680668	6.480908641338005
>50	0.03614679265447518	1.857711889410282
>100	0.015586231655109747	1.9700477774650473
>500	9.94865850326154E-4	0.5187563557443444
>1k	9.94865850326154E-4	1.2724860395279713
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2136	0.45456383365857916	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1997	0.42498313474540383	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1055	0.22451537664316526	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	883	0.18791192187290515	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	691	0.14705225143168454	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	541	0.11513063389948096	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	497	0.1057669594233679	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.1281078354802395E-4	0.0
2	0.0	0.0	0.0	2.1281078354802395E-4	0.0
3	0.0	0.0	0.0	2.1281078354802395E-4	0.0
4	0.0	0.0	0.0	2.1281078354802395E-4	0.0
5	0.0	0.0	0.0	2.1281078354802395E-4	0.0
6	0.0	0.0	0.0	2.1281078354802395E-4	0.0
7	0.0	0.0	0.0	2.1281078354802395E-4	0.0
8	0.0	0.0	0.0	2.1281078354802395E-4	0.0
9	0.0	0.0	0.0	2.1281078354802395E-4	0.0
10-11	0.0	0.0	0.0	2.1281078354802395E-4	2.1281078354802395E-4
12-13	0.0	0.0	0.0	6.384323506440718E-4	4.256215670960479E-4
14-15	0.0	0.0	0.0	6.384323506440718E-4	4.256215670960479E-4
16-17	0.0	0.0	0.0	7.448377424180838E-4	4.256215670960479E-4
18-19	0.0	0.0	0.0	8.512431341920958E-4	4.256215670960479E-4
20-21	0.0	0.0	0.0	0.0013832700930621557	4.256215670960479E-4
22-23	0.0	0.0	0.0	0.0034049725367683832	5.320269588700599E-4
24-25	0.0	0.0	0.0	0.010002106826757125	6.384323506440718E-4
26-27	0.0	0.0	0.0	0.01617361954964982	6.384323506440718E-4
28-29	0.0	0.0	0.0	0.027239780294147066	6.384323506440718E-4
30-31	0.0	0.0	0.0	0.04937210178314155	6.384323506440718E-4
32-33	0.0	0.0	0.0	0.0905509883996842	6.384323506440718E-4
34-35	0.0	0.0	0.0	0.133219550501063	8.512431341920958E-4
36-37	0.0	0.0	0.0	0.19450905616289388	8.512431341920958E-4
38-39	0.0	0.0	0.0	0.27218499215792263	8.512431341920958E-4
40-41	0.0	0.0	0.0	0.36486408839308704	8.512431341920958E-4
42-43	0.0	0.0	0.0	0.44562578074956216	8.512431341920958E-4
44-45	0.0	0.0	0.0	0.523514527528139	0.0011704593095141318
46-47	0.0	0.0	0.0	0.613001462010083	0.0014896754848361677
48-49	0.0	0.0	0.0	0.7056805582452474	0.0014896754848361677
50-51	0.0	0.0	0.0	0.8032543025020165	0.0014896754848361677
52-53	0.0	0.0	0.0	0.8928476423757346	0.0020217024437062274
54-55	0.0	0.0	0.0	0.9750990102170457	0.0021281078354802394
56-57	0.0	0.0	0.0	1.0678845118439841	0.0021281078354802394
58-59	0.0	0.0	0.0	1.1663094992349452	0.0021281078354802394
60-61	0.0	0.0	0.0	1.2632448111410701	0.0021281078354802394
62-63	0.0	0.0	0.0	1.3519869078805962	0.0021281078354802394
64-65	0.0	0.0	0.0	1.4489222197867209	0.0021281078354802394
66-67	0.0	0.0	0.0	1.5585197733139533	0.0021281078354802394
68-69	0.0	0.0	0.0	1.6735440018216603	0.0021281078354802394
70-71	0.0	0.0	0.0	1.7936756891345198	0.0021281078354802394
72-73	0.0	0.0	0.0	1.9051885397136843	0.0021281078354802394
74-75	0.0	0.0	0.0	2.0136156339314026	0.0021281078354802394
76-77	0.0	0.0	0.0	2.1370458883892565	0.0021281078354802394
78-79	0.0	0.0	0.0	2.2728191682928958	0.0021281078354802394
80-81	0.0	0.0	0.0	2.4196586089410324	0.0021281078354802394
82-83	0.0	0.0	0.0	2.5677749142904567	0.0021281078354802394
84-85	0.0	0.0	0.0	2.713443895629079	0.0021281078354802394
86-87	0.0	0.0	0.0	2.862305038720922	0.0021281078354802394
88-89	0.0	0.0	0.0	3.0288294768472506	0.0021281078354802394
90-91	0.0	0.0	0.0	3.202376670830664	0.0021281078354802394
92-93	0.0	0.0	0.0	3.364644893286033	0.0021281078354802394
94-95	0.0	0.0	0.0	3.528722007401559	0.0021281078354802394
96-97	0.0	0.0	0.0	3.7012051474672325	0.0021281078354802394
98-99	0.0	0.0	0.0	3.8795405840804764	0.0021281078354802394
100-101	0.0	0.0	0.0	4.093202610762692	0.0021281078354802394
102-103	0.0	0.0	0.0	4.296862530618151	0.0021281078354802394
104-105	0.0	0.0	0.0	4.491690802956367	0.0021281078354802394
106-107	0.0	0.0	0.0	4.700138965441656	0.0021281078354802394
108-109	0.0	0.0	0.0	4.923909504342404	0.0021281078354802394
110-111	0.0	0.0	0.0	5.15427717753314	0.0021281078354802394
112-113	0.0	0.0	0.0	5.396775065386113	0.0021281078354802394
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGGAT	65	0.0019410303	36.602715	7
GGTATCA	3015	0.0	28.022463	1
GTATCAA	3910	0.0	26.477549	1
CTCAACG	280	9.458745E-11	25.499315	1
CCGCAGA	445	0.0	25.403814	1
GAGTACT	2020	0.0	24.875942	12-13
GTGTTAT	155	2.104071E-4	23.031641	1
ATCAACG	4560	0.0	22.828957	3
CAACGCA	4620	0.0	22.78999	5
TATCAAC	4645	0.0	22.667332	2
AACGCAG	4735	0.0	22.485352	6
TCAACGC	4770	0.0	22.073322	4
GTACTTT	2395	0.0	21.601698	14-15
ACGCAGA	4990	0.0	21.3363	7
CGCAGAG	5200	0.0	20.699001	8
ATCGTAC	75	0.004717608	19.824358	42-43
GCAGAGT	5475	0.0	19.550713	9
AGTACTT	2070	0.0	19.391333	12-13
CATGGGG	1630	0.0	18.977043	4
GTCTTAT	190	8.313191E-4	18.788969	1
>>END_MODULE