FastQCFastQC Report
Sun 1 Jan 2017
ERR1142532_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142532_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences487432
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13100.26875543665577967No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10100.20720839009338737No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19400.031.3208981
GTACTAA1358.16991E-526.4760531
GTATCAA25900.025.530481
CATGGGG18050.025.0799964
ACCGTGT1200.001278632224.8187458
GTACATG43750.024.1007751
ACATGGG43700.023.4443443
TACATGG45050.023.2706742
GCGACCG1300.002031403822.9214089
TATCAAC30850.021.0456522
AACGCAG29600.020.7227427
ATCAACG29600.020.5257743
GTCTTAT1754.7396112E-420.4243851
ATGGGGG10000.020.2520965
CAACGCA30300.020.051585
TCAACGC30100.019.9869254
GTACACT3301.7058483E-819.8549984
ACGCAGA32350.018.7770587
CGCAGAG32700.018.5799058
CATGGGA21300.018.4567574
Produced by FastQC (version 0.11.5)