##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142532_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	487432
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.206590868059546	33.0	33.0	33.0	27.0	33.0
2	31.281413612565444	33.0	33.0	33.0	27.0	33.0
3	31.378889773342742	33.0	33.0	33.0	27.0	33.0
4	31.320315449129314	33.0	33.0	33.0	27.0	33.0
5	31.386462111638135	33.0	33.0	33.0	27.0	33.0
6	34.75643782107043	37.0	37.0	37.0	27.0	37.0
7	34.73392801457434	37.0	37.0	37.0	27.0	37.0
8	34.727726123849074	37.0	37.0	37.0	27.0	37.0
9	34.635627123373105	37.0	37.0	37.0	27.0	37.0
10-11	34.75570746278456	37.0	37.0	37.0	27.0	37.0
12-13	34.68792262305306	37.0	37.0	37.0	27.0	37.0
14-15	34.66464142690673	37.0	37.0	37.0	27.0	37.0
16-17	34.647384660834746	37.0	37.0	37.0	27.0	37.0
18-19	34.695920661753846	37.0	37.0	37.0	27.0	37.0
20-21	34.672709218926954	37.0	37.0	37.0	27.0	37.0
22-23	34.76860054325526	37.0	37.0	37.0	27.0	37.0
24-25	34.86306089875101	37.0	37.0	37.0	27.0	37.0
26-27	34.914101864465195	37.0	37.0	37.0	27.0	37.0
28-29	34.97889654351786	37.0	37.0	37.0	27.0	37.0
30-31	34.917539267015705	37.0	37.0	37.0	27.0	37.0
32-33	34.938656058691265	37.0	37.0	37.0	27.0	37.0
34-35	34.996938034433526	37.0	37.0	37.0	27.0	37.0
36-37	34.97972024815769	37.0	37.0	37.0	27.0	37.0
38-39	34.9599564657224	37.0	37.0	37.0	27.0	37.0
40-41	34.91161946692051	37.0	37.0	37.0	27.0	37.0
42-43	34.93051543599928	37.0	37.0	37.0	27.0	37.0
44-45	34.93315683008092	37.0	37.0	37.0	27.0	37.0
46-47	34.91929540941095	37.0	37.0	37.0	27.0	37.0
48-49	34.9096150847708	37.0	37.0	37.0	27.0	37.0
50-51	34.84731408688802	37.0	37.0	37.0	27.0	37.0
52-53	34.8697685010422	37.0	37.0	37.0	27.0	37.0
54-55	34.85413247386302	37.0	37.0	37.0	27.0	37.0
56-57	34.82876688440644	37.0	37.0	37.0	27.0	37.0
58-59	34.791667350522744	37.0	37.0	37.0	27.0	37.0
60-61	34.77899173628322	37.0	37.0	37.0	27.0	37.0
62-63	34.756082899768586	37.0	37.0	37.0	27.0	37.0
64-65	34.703049861313986	37.0	37.0	37.0	27.0	37.0
66-67	34.66414494739779	37.0	37.0	37.0	27.0	37.0
68-69	34.62399268000459	37.0	37.0	37.0	27.0	37.0
70-71	34.575653629634495	37.0	37.0	37.0	27.0	37.0
72-73	34.51204680858048	37.0	37.0	37.0	27.0	37.0
74-75	34.42633639153769	37.0	37.0	37.0	27.0	37.0
76-77	34.38757303582858	37.0	37.0	37.0	27.0	37.0
78-79	34.34595697451132	37.0	37.0	37.0	27.0	37.0
80-81	34.27877221848381	37.0	37.0	37.0	27.0	37.0
82-83	34.22767996356414	37.0	37.0	37.0	27.0	37.0
84-85	34.17748424395608	37.0	37.0	37.0	27.0	37.0
86-87	34.10429249618409	37.0	37.0	37.0	27.0	37.0
88-89	34.062624119877235	37.0	37.0	37.0	27.0	37.0
90-91	33.980049524856796	37.0	37.0	37.0	27.0	37.0
92-93	33.922193249519935	37.0	37.0	37.0	24.5	37.0
94-95	33.859430648787935	37.0	37.0	37.0	22.0	37.0
96-97	33.77485885210655	37.0	37.0	37.0	22.0	37.0
98-99	33.691407211672605	37.0	33.0	37.0	22.0	37.0
100-101	33.595836957770516	37.0	33.0	37.0	22.0	37.0
102-103	33.50333892727601	37.0	33.0	37.0	22.0	37.0
104-105	33.38223588110752	37.0	33.0	37.0	18.0	37.0
106-107	33.29502474191271	37.0	33.0	37.0	22.0	37.0
108-109	33.190599919578524	37.0	33.0	37.0	14.0	37.0
110-111	33.12488511217975	37.0	33.0	37.0	14.0	37.0
112-113	32.986378612811635	37.0	33.0	37.0	14.0	37.0
114-115	32.87480510101922	37.0	33.0	37.0	14.0	37.0
116-117	32.66891074036994	37.0	33.0	37.0	14.0	37.0
118-119	32.59282833297773	37.0	33.0	37.0	14.0	37.0
120-121	32.48770084852862	37.0	33.0	37.0	14.0	37.0
122-123	32.40116159792545	37.0	33.0	37.0	14.0	37.0
124-125	30.808972328447865	35.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	902.0
1	52.0
2	35.0
3	16.0
4	14.0
5	18.0
6	22.0
7	16.0
8	23.0
9	27.0
10	20.0
11	13.0
12	12.0
13	22.0
14	53.0
15	206.0
16	297.0
17	567.0
18	1211.0
19	2251.0
20	3478.0
21	4072.0
22	4200.0
23	4335.0
24	4534.0
25	5114.0
26	6022.0
27	7108.0
28	8629.0
29	10350.0
30	12252.0
31	15070.0
32	18854.0
33	25579.0
34	39229.0
35	76173.0
36	236656.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.7790125338265	19.971950260108823	12.180424494301494	24.06861271176318
2	17.02633545436018	23.296985616679464	36.24135652395882	23.435322405001532
3	19.52744203662232	28.205687656435252	27.916899947306018	24.349970359636412
4	14.385625488823942	19.73613798213477	34.041699254929405	31.836537274111887
5	15.111788199448265	38.16259727426195	30.941038415613292	15.784576110676493
6	28.994708559296228	36.63623196754364	18.42910868438161	15.939950788778527
7	26.008039630358038	34.0711223389811	21.312220731877915	18.608617298782946
8	25.052662760845234	34.60519386478836	20.502476795471004	19.839666578895397
9	26.392086427144477	19.27863536953941	19.610287809303962	34.71899039401215
10-11	24.60525476625939	28.171282914294764	25.465310766078346	21.7581515533675
12-13	25.544072160174846	26.990300852611238	25.151311226768247	22.314315760445673
14-15	24.075166497312466	27.284763166645558	23.36470246680606	25.27536786923591
16-17	23.51946551019715	29.132966893864626	24.169053394436308	23.178514201501915
18-19	23.597921925048954	28.097536581877446	25.607159871627733	22.697381621445864
20-21	25.47756349251259	26.824422690117444	24.940507117025817	22.75750670034415
22-23	25.21452858237784	26.74310581626827	24.810522471361036	23.23184312999285
24-25	24.147713830170243	27.258344905621563	25.08378336676439	23.510157897443808
26-27	23.790924436827698	27.26433891868038	25.26346866201638	23.68126798247554
28-29	23.78135520521864	27.141956394060113	25.503725029751894	23.57296337096935
30-31	24.211260419838222	26.922887025501346	25.450634859244754	23.415217695415684
32-33	23.62148840577325	27.19716356993565	25.309501670424435	23.871846353866662
34-35	23.731276663142438	27.310874242530208	25.446254098461818	23.511594995865543
36-37	23.941486172235862	27.389453482165173	25.085186103223034	23.583874242375934
38-39	23.89806645487932	27.31007786218876	25.033449579836255	23.758406103095666
40-41	23.61260820195504	27.29764588750288	25.517867722081427	23.57187818846065
42-43	23.668597063369873	27.267341945556023	25.338282224615078	23.725778766459023
44-45	23.673081946509853	27.29332370732831	25.279884323392633	23.753710022769205
46-47	23.50605435446615	27.40112180904564	25.351568043868618	23.74125579261959
48-49	23.60581664268945	27.503699415189043	25.333204449375856	23.55727949274565
50-51	23.269391159299417	27.71072070951029	25.358267113264343	23.661621017925956
52-53	23.273284854393495	27.422172995975856	25.262320016536727	24.042222133093922
54-55	23.366555355238408	27.32121351739657	25.305986443111355	24.006244684253666
56-57	23.564116750548646	27.086028560322006	25.10422687006761	24.245627819061745
58-59	23.598914094152974	27.155050079180636	25.314974394833722	23.931061431832674
60-61	23.64766138245114	27.153813961475304	25.500787131151164	23.697737524922392
62-63	23.26304553933354	27.19475402014236	25.503918854719593	24.038281585804512
64-65	23.262719968412153	26.96298093829882	25.51633581004518	24.257963283243843
66-67	23.674044369039358	26.94316341482224	25.254055165676974	24.128737050461428
68-69	23.657825784717396	26.885131605467414	25.290708435994784	24.166334173820406
70-71	23.46799328328358	26.785837311914975	25.522185626926095	24.22398377787535
72-73	23.716860563749556	26.912435289097726	25.5214143605509	23.849289786601823
74-75	23.811022261710846	26.73071227602547	25.51674564391135	23.941519818352337
76-77	23.672593677489427	26.547097931443055	25.57576480655619	24.204543584511327
78-79	23.65228265094784	26.56007371285306	25.733375016196675	24.05426862000243
80-81	23.568600429877513	26.657856577228834	25.879346339356417	23.894196653537232
82-83	23.696807717502075	26.58069040834438	25.780228888392998	23.942272985760546
84-85	23.611752401969905	26.757785385286976	25.529626266542305	24.10083594620081
86-87	23.50131567717529	26.749737532943342	25.62871271348263	24.120234076398734
88-89	23.559458484088573	26.348057737843707	25.737633957534573	24.354849820533147
90-91	23.784967461370016	26.549054683403757	25.609598124787958	24.05637973043827
92-93	23.6105927554962	26.84852466567348	25.593881313185836	23.947001265644484
94-95	23.099761188321978	26.951486173492622	25.76297915255265	24.185773485632748
96-97	23.351216548638817	26.953318948635523	25.445392801963006	24.25007170076265
98-99	23.17641834129011	27.08993827845832	25.380156997232607	24.353486383018968
100-101	23.285830878377407	27.4858099093191	25.17849396933995	24.049865242963545
102-103	23.390756686913374	27.399975124199116	25.43139469980973	23.777873489077773
104-105	23.220262668793897	27.514843994144638	25.518409019885198	23.746484317176268
106-107	23.243452715023754	27.58802523729347	25.466106660252496	23.702415387430282
108-109	23.23073254169271	27.686897634946217	25.218126048485246	23.864243774875828
110-111	23.07423465873931	28.152387766052584	25.066146947989072	23.70723062721903
112-113	22.996265691957106	28.168379980490734	25.063549021401606	23.771805306150558
114-115	23.057561166126224	28.050649303931795	25.160514509771676	23.731275020170305
116-117	23.121413425237357	28.219887161976732	25.01390175136371	23.644797661422203
118-119	23.026491590852096	27.824803594700036	25.219941047577155	23.928763766870713
120-121	23.008283829061234	27.848568315895495	25.439577380830023	23.703570474213244
122-123	23.010818549079133	28.00839832650087	25.324674657340672	23.65610846707932
124-125	23.095397877398	27.932965486906646	25.043291667137694	23.92834496855766
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1748.0
1	1056.5
2	314.5
3	229.0
4	190.0
5	165.0
6	127.0
7	120.5
8	132.0
9	123.0
10	108.0
11	99.0
12	106.5
13	119.5
14	137.0
15	149.5
16	164.0
17	196.0
18	237.5
19	288.0
20	359.0
21	453.5
22	600.0
23	772.5
24	961.0
25	1228.0
26	1568.5
27	2022.0
28	2538.0
29	3305.0
30	4136.0
31	4961.5
32	6015.0
33	6989.0
34	7913.5
35	8806.0
36	9722.5
37	10783.0
38	11505.5
39	11989.5
40	12378.0
41	12595.5
42	12962.5
43	13756.5
44	14901.0
45	15919.0
46	17157.5
47	18250.5
48	19082.0
49	20064.0
50	20480.0
51	19897.0
52	19131.0
53	18743.0
54	18272.5
55	17510.5
56	16481.0
57	15072.0
58	13748.0
59	12371.0
60	10861.5
61	9163.0
62	7418.0
63	5924.5
64	4570.5
65	3612.5
66	2859.5
67	2252.5
68	1850.0
69	1567.5
70	1315.5
71	1179.0
72	1006.0
73	835.0
74	679.0
75	573.5
76	453.5
77	299.5
78	206.0
79	140.5
80	103.0
81	77.5
82	57.0
83	36.5
84	21.0
85	10.5
86	5.5
87	4.5
88	2.5
89	0.0
90	1.5
91	2.0
92	1.0
93	1.0
94	1.5
95	1.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.38282262961808006
2	0.34076548113377864
3	0.3298921695744227
4	0.32250652398693563
5	0.34712534261189254
6	0.2798339050370103
7	0.2749101413120189
8	0.2708070048745261
9	0.2833215710088792
10-11	0.27880812092763707
12-13	0.31040227149633176
14-15	0.36179405537592935
16-17	0.3252761410822433
18-19	0.30804296804477344
20-21	0.2973748133072921
22-23	0.29224589276042606
24-25	0.2783978072838878
26-27	0.2807571107354462
28-29	0.2725508378604605
30-31	0.2749101413120189
32-33	0.2724482594495232
34-35	0.2613697910682926
36-37	0.2647548786292242
38-39	0.2569589193979878
40-41	0.26752449572453185
42-43	0.25901048761673423
44-45	0.2562408705214266
46-47	0.2570614978089251
48-49	0.24752170559175432
50-51	0.2538815670698682
52-53	0.25470219435736674
54-55	0.2581898603292357
56-57	0.2529583613714323
58-59	0.24659849989331845
60-61	0.24054637364801656
62-63	0.24475208849644667
64-65	0.2398283247714553
66-67	0.23993090318239263
68-69	0.24741912718081704
70-71	0.24259794186676295
72-73	0.2327504144167802
74-75	0.24947069539956343
76-77	0.2658832411495347
78-79	0.25049647950893666
80-81	0.2558305568776773
82-83	0.2487526465230022
84-85	0.24957327381050073
86-87	0.24218762822301368
88-89	0.2319297871292816
90-91	0.22300546537773475
92-93	0.22946790526678593
94-95	0.21859459370742995
96-97	0.21243988905119074
98-99	0.2162352902558716
100-101	0.20536197869651562
102-103	0.2083367526136979
104-105	0.2133630947496266
106-107	0.20700323327151274
108-109	0.20843933102463524
110-111	0.20823417420276058
112-113	0.20443877299807975
114-115	0.1947964023699716
116-117	0.20156657749183476
118-119	0.19243709891841324
120-121	0.19325772620591178
122-123	0.18597465902936205
124-125	0.18597465902936205
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	487432.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.95436205263088
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.9524284662316	70.32180308997201
2	8.773125577640904	14.028993175357948
3	1.7474524346462172	4.1914933388836495
4	0.5624912382131214	1.7989451244609826
5	0.27450545424574285	1.0973954237093013
6	0.1621520191684628	0.7778856748896251
7	0.10161565990456384	0.5687230683558554
8	0.06749358360395062	0.4317125135759824
9	0.04726110978789438	0.34008586946914005
>10	0.28728349793100294	4.204039946713156
>50	0.01770070155315101	0.9562360970334906
>100	0.0059002337030825795	0.7354573484168914
>500	0.0	0.0
>1k	5.900233703082562E-4	0.547229329162022
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1310	0.26875543665577967	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	1010	0.20720839009338737	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	1.0257841093732049E-4	0.0
12-13	0.0	0.0	0.0	2.0515682187464097E-4	0.0
14-15	0.0	0.0	0.0	2.0515682187464097E-4	2.0515682187464097E-4
16-17	0.0	0.0	0.0	3.0773523281196145E-4	2.0515682187464097E-4
18-19	0.0	0.0	0.0	5.128920546866024E-4	2.0515682187464097E-4
20-21	0.0	0.0	0.0	0.0014360977531224868	2.0515682187464097E-4
22-23	0.0	0.0	0.0	0.003897979615618178	2.0515682187464097E-4
24-25	0.0	0.0	0.0	0.011591360435917214	2.0515682187464097E-4
26-27	0.0	0.0	0.0	0.01866927079059233	2.0515682187464097E-4
28-29	0.0	0.0	0.0	0.0335431403765038	2.0515682187464097E-4
30-31	0.0	0.0	0.0	0.06195736020614158	2.0515682187464097E-4
32-33	0.0	0.0	0.0	0.1046299791560669	2.0515682187464097E-4
34-35	0.0	0.0	0.0	0.1502773720231745	2.0515682187464097E-4
36-37	0.0	0.0	0.0	0.21808170165274338	2.0515682187464097E-4
38-39	0.0	0.0	0.0	0.3133770454135141	2.0515682187464097E-4
40-41	0.0	0.0	0.0	0.41410904495396283	2.0515682187464097E-4
42-43	0.0	0.0	0.0	0.49760787145694174	2.0515682187464097E-4
44-45	0.0	0.0	0.0	0.5819273252474191	2.0515682187464097E-4
46-47	0.0	0.0	0.0	0.6807103349800587	2.0515682187464097E-4
48-49	0.0	0.0	0.0	0.7759031003298922	2.0515682187464097E-4
50-51	0.0	0.0	0.0	0.8644282689687998	2.0515682187464097E-4
52-53	0.0	0.0	0.0	0.9592107206748839	2.0515682187464097E-4
54-55	0.0	0.0	0.0	1.0520441825731588	2.0515682187464097E-4
56-57	0.0	0.0	0.0	1.1539045446339182	2.0515682187464097E-4
58-59	0.0	0.0	0.0	1.2653046989118482	2.0515682187464097E-4
60-61	0.0	0.0	0.0	1.3778332157100888	2.0515682187464097E-4
62-63	0.0	0.0	0.0	1.4865663313036483	2.0515682187464097E-4
64-65	0.0	0.0	0.0	1.5886318501862824	2.0515682187464097E-4
66-67	0.0	0.0	0.0	1.6991087987657765	2.0515682187464097E-4
68-69	0.0	0.0	0.0	1.8261008715061793	2.0515682187464097E-4
70-71	0.0	0.0	0.0	1.9660178240246844	2.0515682187464097E-4
72-73	0.0	0.0	0.0	2.096292405915081	2.0515682187464097E-4
74-75	0.0	0.0	0.0	2.2126163239180032	2.0515682187464097E-4
76-77	0.0	0.0	0.0	2.347917247944329	2.0515682187464097E-4
78-79	0.0	0.0	0.0	2.4985023552003147	2.0515682187464097E-4
80-81	0.0	0.0	0.0	2.6599607740156577	2.0515682187464097E-4
82-83	0.0	0.0	0.0	2.826548113377866	2.0515682187464097E-4
84-85	0.0	0.0	0.0	2.9870833264947727	2.0515682187464097E-4
86-87	0.0	0.0	0.0	3.1487469021319896	2.0515682187464097E-4
88-89	0.0	0.0	0.0	3.333182884997292	2.0515682187464097E-4
90-91	0.0	0.0	0.0	3.5202859065469645	2.0515682187464097E-4
92-93	0.0	0.0	0.0	3.6981568711122783	2.0515682187464097E-4
94-95	0.0	0.0	0.0	3.8700782878432274	2.0515682187464097E-4
96-97	0.0	0.0	0.0	4.0573864662147745	2.0515682187464097E-4
98-99	0.0	0.0	0.0	4.2636716506097265	2.0515682187464097E-4
100-101	0.0	0.0	0.0	4.476521853304666	2.0515682187464097E-4
102-103	0.0	0.0	0.0	4.700655681202711	2.0515682187464097E-4
104-105	0.0	0.0	0.0	4.915967765760147	2.0515682187464097E-4
106-107	0.0	0.0	0.0	5.13292110489258	2.0515682187464097E-4
108-109	0.0	0.0	0.0	5.368954028459354	2.0515682187464097E-4
110-111	0.0	0.0	0.0	5.625194898980781	2.0515682187464097E-4
112-113	0.0	0.0	0.0	5.891078140130316	2.0515682187464097E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1940	0.0	31.320898	1
GTACTAA	135	8.16991E-5	26.476053	1
GTATCAA	2590	0.0	25.53048	1
CATGGGG	1805	0.0	25.079996	4
ACCGTGT	120	0.0012786322	24.818745	8
GTACATG	4375	0.0	24.100775	1
ACATGGG	4370	0.0	23.444344	3
TACATGG	4505	0.0	23.270674	2
GCGACCG	130	0.0020314038	22.921408	9
TATCAAC	3085	0.0	21.045652	2
AACGCAG	2960	0.0	20.722742	7
ATCAACG	2960	0.0	20.525774	3
GTCTTAT	175	4.7396112E-4	20.424385	1
ATGGGGG	1000	0.0	20.252096	5
CAACGCA	3030	0.0	20.05158	5
TCAACGC	3010	0.0	19.986925	4
GTACACT	330	1.7058483E-8	19.854998	4
ACGCAGA	3235	0.0	18.777058	7
CGCAGAG	3270	0.0	18.579905	8
CATGGGA	2130	0.0	18.456757	4
>>END_MODULE