FastQCFastQC Report
Sun 1 Jan 2017
ERR1142532_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142532_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences487432
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28050.5754648853583679No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23990.49217121567726374No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14350.2944000393901098No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6930.14217367755912622No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5380.11037437016855685No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA5050.10360419504669369No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25000.038.0984951
GTATCAA35950.035.9326061
CTCAACG2109.094947E-1131.1818031
ATCAACG43550.030.0565743
CGTTAAC800.00534649929.764451
TATCAAC44250.029.7155652
TCAACGC45450.029.0619034
CAACGCA45600.028.9663055
AACGCAG47350.028.3954586
CATGGGG16550.025.5249774
ACGCAGA51550.025.5023087
CGCAGAG53300.024.6624588
GTGTTAT2201.5277146E-724.3527341
CCGCAGA3451.8189894E-1224.1566541
GCAGAGT55950.023.4943529
ATGGGGG9950.023.3209655
ACATGGG42000.022.8077053
GAGTACT24950.022.7669112-13
TACATGG44400.022.244882
GTACATG43500.021.8956871
Produced by FastQC (version 0.11.5)