##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142532_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	487432
Sequences flagged as poor quality	0
Sequence length	125
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	21.867638973231138	14.0	14.0	33.0	14.0	33.0
2	27.622080618424725	27.0	27.0	33.0	14.0	33.0
3	31.179998440808152	33.0	33.0	33.0	27.0	33.0
4	30.821117612302846	33.0	33.0	33.0	27.0	33.0
5	32.29714298609857	33.0	33.0	33.0	33.0	33.0
6	34.9535545470958	37.0	37.0	37.0	33.0	37.0
7	35.087601962940475	37.0	37.0	37.0	33.0	37.0
8	35.42627279292291	37.0	37.0	37.0	33.0	37.0
9	35.89951624021402	37.0	37.0	37.0	33.0	37.0
10-11	36.00319121436426	37.0	37.0	37.0	33.0	37.0
12-13	36.073370234207026	37.0	37.0	37.0	37.0	37.0
14-15	36.078512489947315	37.0	37.0	37.0	37.0	37.0
16-17	36.09259035106435	37.0	37.0	37.0	37.0	37.0
18-19	36.08018759539792	37.0	37.0	37.0	37.0	37.0
20-21	36.10766424855159	37.0	37.0	37.0	37.0	37.0
22-23	36.10429147039997	37.0	37.0	37.0	37.0	37.0
24-25	36.09381821464327	37.0	37.0	37.0	37.0	37.0
26-27	36.04638185428942	37.0	37.0	37.0	37.0	37.0
28-29	36.00969571140179	37.0	37.0	37.0	37.0	37.0
30-31	35.927145735199986	37.0	37.0	37.0	35.0	37.0
32-33	35.8699551937501	37.0	37.0	37.0	33.0	37.0
34-35	35.79540838516962	37.0	37.0	37.0	33.0	37.0
36-37	35.72029431797666	37.0	37.0	37.0	33.0	37.0
38-39	35.65518062006598	37.0	37.0	37.0	33.0	37.0
40-41	35.523134509018696	37.0	37.0	37.0	33.0	37.0
42-43	35.46359902509478	37.0	37.0	37.0	33.0	37.0
44-45	35.36189663378687	37.0	37.0	37.0	33.0	37.0
46-47	35.20410847051486	37.0	37.0	37.0	33.0	37.0
48-49	35.05788910042837	37.0	37.0	37.0	33.0	37.0
50-51	34.93983263306471	37.0	37.0	37.0	27.0	37.0
52-53	34.85788889527154	37.0	37.0	37.0	27.0	37.0
54-55	34.79973206519063	37.0	37.0	37.0	27.0	37.0
56-57	34.739684714996145	37.0	37.0	37.0	27.0	37.0
58-59	34.687766703868434	37.0	37.0	37.0	27.0	37.0
60-61	34.64089452477474	37.0	37.0	37.0	27.0	37.0
62-63	34.63436233156625	37.0	37.0	37.0	27.0	37.0
64-65	34.59577951386039	37.0	37.0	37.0	27.0	37.0
66-67	34.54837495281393	37.0	37.0	37.0	27.0	37.0
68-69	34.50799906448489	37.0	37.0	37.0	27.0	37.0
70-71	34.48108043788672	37.0	37.0	37.0	27.0	37.0
72-73	34.447863496856996	37.0	37.0	37.0	27.0	37.0
74-75	34.34627701915344	37.0	37.0	37.0	27.0	37.0
76-77	34.28518644651972	37.0	37.0	37.0	27.0	37.0
78-79	34.26777170969489	37.0	37.0	37.0	27.0	37.0
80-81	34.225646859459374	37.0	37.0	37.0	27.0	37.0
82-83	34.1715777790543	37.0	37.0	37.0	27.0	37.0
84-85	34.07466785110539	37.0	37.0	37.0	27.0	37.0
86-87	33.899290567709954	37.0	37.0	37.0	24.5	37.0
88-89	33.833176730292635	37.0	37.0	37.0	22.0	37.0
90-91	33.78384369512055	37.0	37.0	37.0	22.0	37.0
92-93	33.72509909074496	37.0	35.0	37.0	22.0	37.0
94-95	33.732906333601406	37.0	33.0	37.0	22.0	37.0
96-97	33.682721897618535	37.0	33.0	37.0	22.0	37.0
98-99	33.60001395066389	37.0	33.0	37.0	22.0	37.0
100-101	33.536457393031235	37.0	33.0	37.0	22.0	37.0
102-103	33.427953027294066	37.0	33.0	37.0	14.0	37.0
104-105	33.32039751185806	37.0	33.0	37.0	14.0	37.0
106-107	33.243910945526764	37.0	33.0	37.0	14.0	37.0
108-109	33.131654261517504	37.0	33.0	37.0	14.0	37.0
110-111	33.06549528959937	37.0	33.0	37.0	14.0	37.0
112-113	32.99492954914737	37.0	33.0	37.0	14.0	37.0
114-115	32.87485023552003	37.0	33.0	37.0	14.0	37.0
116-117	32.74775250701636	37.0	33.0	37.0	14.0	37.0
118-119	32.585274458796306	37.0	33.0	37.0	14.0	37.0
120-121	32.49459616931182	37.0	33.0	37.0	14.0	37.0
122-123	32.346251374550704	37.0	33.0	37.0	14.0	37.0
124-125	31.069940012145285	35.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	9.0
16	6.0
17	13.0
18	81.0
19	432.0
20	1505.0
21	3524.0
22	5491.0
23	7042.0
24	5895.0
25	4170.0
26	4108.0
27	4839.0
28	6182.0
29	7831.0
30	10523.0
31	14572.0
32	20512.0
33	30934.0
34	50954.0
35	103819.0
36	204990.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	44.318422008483424	12.980014487905883	12.590934182633804	30.11062932097689
2	17.259227953847923	20.61477293242955	38.648467888854235	23.47753122486829
3	20.394807027909764	26.581772077106773	28.688749928194525	24.33467096678894
4	14.319741010028064	18.745178814685946	34.25770158709317	32.67737858819281
5	16.18508138804886	35.118522500194906	32.31312754145313	16.38326857030311
6	29.51619124212185	34.781627494747895	19.008091517857142	16.694089745273107
7	26.00915078274072	32.16910482365252	22.94835757812019	18.87338681548657
8	25.78739188235487	31.884857785291075	21.389650248650067	20.938100083703983
9	26.895649034121682	16.279193815752762	21.267376782812782	35.55778036731277
10-11	25.115400712304485	25.447959920563278	26.845077877529583	22.59156148960265
12-13	26.542574143675424	24.348729668958953	27.02171790116365	22.086978286201973
14-15	24.571427399103875	24.569068095652316	26.058506622462208	24.800997882781598
16-17	23.105274171576752	26.745063926865697	26.908984227543538	23.240677674014016
18-19	22.93807136174892	25.062265095438953	29.613361453495052	22.386302089317073
20-21	24.323032388173914	24.055097029117967	29.56982629336255	22.052044289345567
22-23	24.10500336457188	23.999039866073627	29.41753926701571	22.478417502338786
24-25	23.182105401368805	24.73401418043953	29.484317812535902	22.599562605655763
26-27	22.82205517897881	24.830848200364358	29.61069441481068	22.736402205846147
28-29	22.774766531536706	24.613484547588175	29.940586584385105	22.671162336490013
30-31	23.34776953503258	24.3087240886934	29.903863513269542	22.43964286300448
32-33	22.787999146547623	24.540551297411742	29.711939306405817	22.95951024963482
34-35	22.974099950454576	24.567759777527716	29.709405321568262	22.74873495044945
36-37	22.97307416529297	24.72716679164149	29.41931327786571	22.880445765199827
38-39	22.90329728044117	24.671031036124013	29.378867206092334	23.04680447734248
40-41	22.728606276709456	24.75519109763428	29.596488456590915	22.919714169065347
42-43	23.151071638857605	24.52100912744573	29.359437540903226	22.96848169279344
44-45	22.944328644816096	24.891779776461124	28.9156231022994	23.248268476423377
46-47	22.916752152183367	24.755340451199395	28.866954445110093	23.460952951507146
48-49	23.02186613595679	25.13205340610077	28.575854410414497	23.27022604752794
50-51	22.92083818871145	25.317275025029133	28.332464836120728	23.429421950138686
52-53	22.96915262026293	25.179204483907498	28.07899358269461	23.77264931313496
54-55	23.014081964253474	25.153252145940357	27.982775033235406	23.849890856570763
56-57	23.093169919086147	25.09478245170608	27.72530322178273	24.086744407425037
58-59	22.951919447225457	25.106681547374816	28.06688933020401	23.87450967519572
60-61	22.997874575325376	25.098988166554513	28.202190254230334	23.700947003889773
62-63	22.803283329777283	25.157355282377853	28.28384266933644	23.755518718508426
64-65	22.72901656025866	24.93804263979386	28.395755715669058	23.937185084278422
66-67	22.9377694158971	24.959222153603736	28.254174728198983	23.84883370230018
68-69	22.948739516486402	24.796381854289418	28.43750512892055	23.81737350030363
70-71	22.709526662180572	24.804793283986278	28.810685387910517	23.67499466592263
72-73	22.838672881550657	25.055802655549904	28.745650675376254	23.359873787523185
74-75	22.849593712917834	24.86388664200339	28.89462814159074	23.391891503488033
76-77	22.800365323044087	24.823906083244395	28.78222231343896	23.593506280272557
78-79	22.723957569335525	25.07075367667801	28.78533452461428	23.419954229372188
80-81	22.521808170165276	25.14443040259975	28.979221717080538	23.35453971015444
82-83	22.82359385514287	24.989537002084393	28.975734051108663	23.21113509166407
84-85	22.74107809114414	25.070232864156246	28.59044668820098	23.59824235649863
86-87	22.50830231848218	25.251484961266122	28.654227835559105	23.58598488469259
88-89	22.752716868900475	24.842734643817998	28.596645074271194	23.807903413010333
90-91	22.917201665704525	24.839911167903754	28.4452739640193	23.797613202372418
92-93	22.689986054373566	25.2375788420817	28.33467860362997	23.737756499914767
94-95	22.392354215562378	25.032927669910883	28.50212952781106	24.072588586715685
96-97	22.545093469448048	25.072112622888937	28.134488503011703	24.248305404651315
98-99	22.48231548195441	25.186590129494984	27.890146728159003	24.440947660391604
100-101	22.70008944837434	25.338611334504098	27.676886211820317	24.284413005301253
102-103	22.6914728618556	25.32773802294474	27.83403633737629	24.14675277782337
104-105	22.738826976102942	25.478228400735293	27.62122915572479	24.161715467436977
106-107	22.615883475904177	25.647980123856605	27.571276018452316	24.164860381786905
108-109	22.756205421851742	25.643982097446433	27.291956673604606	24.307855807097216
110-111	22.625323251875397	25.966114073932744	27.172442373717637	24.236120300474223
112-113	22.557710613993336	26.000549820282625	27.13157937927752	24.31016018644652
114-115	22.522857057863515	26.01688647533038	27.204643113955502	24.255613352850606
116-117	22.56696581981263	25.849273179923742	27.2109004034413	24.372860596822324
118-119	22.518423082604343	25.817242199937635	27.23292684928359	24.431407868174432
120-121	22.31392750973984	25.792060825019337	27.460604680457333	24.43340698478349
122-123	22.34393720559996	26.017885571731032	27.332325329481854	24.305851893187153
124-125	22.336551560012474	25.90227970260467	27.41602931280671	24.345139424576146
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	156.0
1	150.5
2	319.0
3	453.5
4	343.5
5	292.5
6	386.5
7	789.5
8	1339.0
9	1531.0
10	1316.0
11	1074.5
12	1005.5
13	1020.5
14	1021.5
15	995.5
16	999.5
17	1052.5
18	1079.0
19	1021.0
20	995.5
21	1023.5
22	1071.5
23	1150.0
24	1260.0
25	1403.5
26	1621.0
27	1988.5
28	2361.5
29	2906.0
30	3681.0
31	4490.0
32	5380.0
33	6367.5
34	7240.5
35	7996.0
36	8881.0
37	9908.5
38	10759.5
39	11508.5
40	11797.0
41	11918.5
42	12546.0
43	13315.0
44	14298.0
45	15366.0
46	16605.0
47	17658.5
48	18729.5
49	19848.0
50	20440.0
51	19748.0
52	18651.5
53	18288.0
54	18049.0
55	17488.0
56	16342.0
57	14969.5
58	13891.5
59	12593.5
60	11057.5
61	9278.5
62	7477.0
63	5958.0
64	4475.0
65	3537.0
66	2822.0
67	2160.0
68	1728.0
69	1449.5
70	1230.0
71	1044.0
72	919.0
73	895.5
74	714.5
75	540.5
76	446.5
77	311.5
78	200.5
79	128.5
80	100.5
81	66.5
82	40.5
83	20.5
84	11.0
85	7.5
86	4.5
87	2.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.026465230021828684
2	0.0
3	8.206272874985639E-4
4	0.0
5	0.0028721955062449736
6	0.0016412545749971278
7	0.008616586518734922
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	2.0515682187464097E-4
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	1.0257841093732049E-4
36-37	1.0257841093732049E-4
38-39	0.0
40-41	0.0022567250406210506
42-43	2.0515682187464097E-4
44-45	0.0
46-47	0.004103136437492819
48-49	0.007077910354675114
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.007180488765612433
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.06934300579362865
76-77	0.039390109799931065
78-79	3.0773523281196145E-4
80-81	0.0
82-83	0.0
84-85	0.06205993861707889
86-87	0.19930985245121374
88-89	0.22003069146055246
90-91	0.1376602274778841
92-93	0.11098984063418077
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
102-103	0.0
104-105	0.0016412545749971278
106-107	0.002769617095307653
108-109	0.003179930739056935
110-111	5.128920546866024E-4
112-113	0.0
114-115	1.0257841093732049E-4
116-117	1.0257841093732049E-4
118-119	0.0
120-121	2.0515682187464097E-4
122-123	0.0
124-125	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	487432.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.10759639386873
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.43322025161999	67.41765458578143
2	9.14674772357326	14.10567463571649
3	1.75779075755872	4.0661706083613165
4	0.6222183908500951	1.9191105820204637
5	0.2734884839856887	1.0544019820769757
6	0.16244840003286926	0.751560339873853
7	0.10403789715418821	0.5615478528402379
8	0.07340166683626564	0.45278608808383924
9	0.06078837447062088	0.42185208997079904
>10	0.32890062219953103	4.6862480723517255
>50	0.023135953396476595	1.1822774533386486
>100	0.012018676802033338	1.4403720920014524
>500	9.014007601524971E-4	0.4022013755371587
>1k	9.014007601524971E-4	1.53814224204562
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2805	0.5754648853583679	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2399	0.49217121567726374	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1435	0.2944000393901098	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	693	0.14217367755912622	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	538	0.11037437016855685	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	505	0.10360419504669369	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	1.0257841093732049E-4	0.0
12-13	0.0	0.0	0.0	2.0515682187464097E-4	0.0
14-15	0.0	0.0	0.0	2.0515682187464097E-4	0.0
16-17	0.0	0.0	0.0	3.0773523281196145E-4	0.0
18-19	0.0	0.0	0.0	5.128920546866024E-4	0.0
20-21	0.0	0.0	0.0	0.0014360977531224868	0.0
22-23	0.0	0.0	0.0	0.003897979615618178	0.0
24-25	0.0	0.0	0.0	0.011591360435917214	0.0
26-27	0.0	0.0	0.0	0.018464113968717687	0.0
28-29	0.0	0.0	0.0	0.03313282673275452	0.0
30-31	0.0	0.0	0.0	0.06164962497332961	0.0
32-33	0.0	0.0	0.0	0.10309130299200708	0.0
34-35	0.0	0.0	0.0	0.14853353903724006	0.0
36-37	0.0	0.0	0.0	0.21531208455743572	0.0
38-39	0.0	0.0	0.0	0.3097868010307079	0.0
40-41	0.0	0.0	0.0	0.4110316926258432	0.0
42-43	0.0	0.0	0.0	0.4950434111835087	0.0
44-45	0.0	0.0	0.0	0.5788499729192995	0.0
46-47	0.0	0.0	0.0	0.6780432962956884	0.0
48-49	0.0	0.0	0.0	0.7743644241658323	0.0
50-51	0.0	0.0	0.0	0.8646334257906744	0.0
52-53	0.0	0.0	0.0	0.9595184559076959	0.0
54-55	0.0	0.0	0.0	1.052351917805971	0.0
56-57	0.0	0.0	0.0	1.15421227986673	0.0
58-59	0.0	0.0	0.0	1.2661253261993468	0.0
60-61	0.0	0.0	0.0	1.3787564214085246	0.0
62-63	0.0	0.0	0.0	1.4887204779333323	0.0
64-65	0.0	0.0	0.0	1.5911963104597153	0.0
66-67	0.0	0.0	0.0	1.702699043148583	0.0
68-69	0.0	0.0	0.0	1.830204007943672	0.0
70-71	0.0	0.0	0.0	1.9709415877496759	0.0
72-73	0.0	0.0	0.0	2.0997800718869506	2.0515682187464097E-4
74-75	0.0	0.0	0.0	2.2148730489586237	2.0515682187464097E-4
76-77	0.0	0.0	0.0	2.350789443450574	2.0515682187464097E-4
78-79	0.0	0.0	0.0	2.5012719722956227	2.0515682187464097E-4
80-81	0.0	0.0	0.0	2.66139687176878	2.0515682187464097E-4
82-83	0.0	0.0	0.0	2.8279842111309885	4.1031364374928195E-4
84-85	0.0	0.0	0.0	2.9886220026588326	4.1031364374928195E-4
86-87	0.0	0.0	0.0	3.1492597941866762	4.1031364374928195E-4
88-89	0.0	0.0	0.0	3.333593198641041	4.1031364374928195E-4
90-91	0.0	0.0	0.0	3.521722004300087	4.1031364374928195E-4
92-93	0.0	0.0	0.0	3.6991826552216516	6.154704656239229E-4
94-95	0.0	0.0	0.0	3.8723350128838483	6.154704656239229E-4
96-97	0.0	0.0	0.0	4.059335456022584	6.154704656239229E-4
98-99	0.0	0.0	0.0	4.266441267705034	6.154704656239229E-4
100-101	0.0	0.0	0.0	4.478983735167162	6.154704656239229E-4
102-103	0.0	0.0	0.0	4.704040768763643	6.154704656239229E-4
104-105	0.0	0.0	0.0	4.920481215841389	6.154704656239229E-4
106-107	0.0	0.0	0.0	5.139280966370693	6.154704656239229E-4
108-109	0.0	0.0	0.0	5.375211311526531	6.154704656239229E-4
110-111	0.0	0.0	0.0	5.633606328677642	6.154704656239229E-4
112-113	0.0	0.0	0.0	5.899181834594364	6.154704656239229E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2500	0.0	38.098495	1
GTATCAA	3595	0.0	35.932606	1
CTCAACG	210	9.094947E-11	31.181803	1
ATCAACG	4355	0.0	30.056574	3
CGTTAAC	80	0.005346499	29.76445	1
TATCAAC	4425	0.0	29.715565	2
TCAACGC	4545	0.0	29.061903	4
CAACGCA	4560	0.0	28.966305	5
AACGCAG	4735	0.0	28.395458	6
CATGGGG	1655	0.0	25.524977	4
ACGCAGA	5155	0.0	25.502308	7
CGCAGAG	5330	0.0	24.662458	8
GTGTTAT	220	1.5277146E-7	24.352734	1
CCGCAGA	345	1.8189894E-12	24.156654	1
GCAGAGT	5595	0.0	23.494352	9
ATGGGGG	995	0.0	23.320965	5
ACATGGG	4200	0.0	22.807705	3
GAGTACT	2495	0.0	22.76691	12-13
TACATGG	4440	0.0	22.24488	2
GTACATG	4350	0.0	21.895687	1
>>END_MODULE