FastQCFastQC Report
Sun 1 Jan 2017
ERR1142531_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142531_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences595179
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12770.214557301248868No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8620.1448303787600033No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29650.028.9401841
CACGATT850.00717606528.0251622
TAACGCA1107.706243E-427.0697564
GGTATCA21650.025.8722041
AACGCAG34100.025.653146
ATCAACG34250.025.2124183
CAACGCA34350.025.1368985
TATCAAC36000.024.4830912
TCAACGC35100.024.432194
CATGGGG22550.024.0326614
ACGCAGA37150.023.2364487
CGCAGAG38150.022.9414028
GCAGAGT40550.021.30799
GTACATG48900.021.0814631
GCTTATG1703.8912223E-421.0312861
ACATGGG48200.021.0043333
TACATGG49950.020.6261232
TCTAGAC1500.00467888519.8511563
ATGGGGA8250.019.488585
ATGGGGG11150.019.2264045
Produced by FastQC (version 0.11.5)