##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142531_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	595179
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.20914380379684	33.0	33.0	33.0	27.0	33.0
2	31.2939317415433	33.0	33.0	33.0	27.0	33.0
3	31.39972008420996	33.0	33.0	33.0	27.0	33.0
4	31.377558684026152	33.0	33.0	33.0	27.0	33.0
5	31.375792828712033	33.0	33.0	33.0	27.0	33.0
6	34.73262497500752	37.0	37.0	37.0	27.0	37.0
7	34.72823805947454	37.0	37.0	37.0	27.0	37.0
8	34.728891644362456	37.0	37.0	37.0	27.0	37.0
9	34.69548824807327	37.0	37.0	37.0	27.0	37.0
10-11	34.7643381234889	37.0	37.0	37.0	27.0	37.0
12-13	34.700488424490786	37.0	37.0	37.0	27.0	37.0
14-15	34.684740221009136	37.0	37.0	37.0	27.0	37.0
16-17	34.65500630902636	37.0	37.0	37.0	27.0	37.0
18-19	34.69560922008337	37.0	37.0	37.0	27.0	37.0
20-21	34.68037262739445	37.0	37.0	37.0	27.0	37.0
22-23	34.75738307299149	37.0	37.0	37.0	27.0	37.0
24-25	34.829636966358024	37.0	37.0	37.0	27.0	37.0
26-27	34.8626287217795	37.0	37.0	37.0	27.0	37.0
28-29	34.88906446632022	37.0	37.0	37.0	27.0	37.0
30-31	34.83758835577196	37.0	37.0	37.0	27.0	37.0
32-33	34.85292911880292	37.0	37.0	37.0	27.0	37.0
34-35	34.90732451917826	37.0	37.0	37.0	27.0	37.0
36-37	34.88913419324271	37.0	37.0	37.0	27.0	37.0
38-39	34.86536991392505	37.0	37.0	37.0	27.0	37.0
40-41	34.82413442006522	37.0	37.0	37.0	27.0	37.0
42-43	34.825260131825885	37.0	37.0	37.0	27.0	37.0
44-45	34.81025456207293	37.0	37.0	37.0	27.0	37.0
46-47	34.79615964272933	37.0	37.0	37.0	27.0	37.0
48-49	34.793061415137295	37.0	37.0	37.0	27.0	37.0
50-51	34.71060806916911	37.0	37.0	37.0	27.0	37.0
52-53	34.72026398780871	37.0	37.0	37.0	27.0	37.0
54-55	34.69189185102297	37.0	37.0	37.0	27.0	37.0
56-57	34.65817258337408	37.0	37.0	37.0	27.0	37.0
58-59	34.61882055650484	37.0	37.0	37.0	27.0	37.0
60-61	34.59878372724844	37.0	37.0	37.0	27.0	37.0
62-63	34.58392265184088	37.0	37.0	37.0	27.0	37.0
64-65	34.54259642897347	37.0	37.0	37.0	27.0	37.0
66-67	34.487691938055605	37.0	37.0	37.0	27.0	37.0
68-69	34.46210635791921	37.0	37.0	37.0	27.0	37.0
70-71	34.40471017962663	37.0	37.0	37.0	27.0	37.0
72-73	34.34688387863147	37.0	37.0	37.0	27.0	37.0
74-75	34.27410325297095	37.0	37.0	37.0	27.0	37.0
76-77	34.228240579724755	37.0	37.0	37.0	27.0	37.0
78-79	34.170878844851714	37.0	37.0	37.0	27.0	37.0
80-81	34.11600207668617	37.0	37.0	37.0	27.0	37.0
82-83	34.06411348518681	37.0	37.0	37.0	27.0	37.0
84-85	34.01375384548178	37.0	37.0	37.0	27.0	37.0
86-87	33.942921373233936	37.0	37.0	37.0	27.0	37.0
88-89	33.894400676099124	37.0	37.0	37.0	27.0	37.0
90-91	33.78342817874959	37.0	37.0	37.0	22.0	37.0
92-93	33.71682636652167	37.0	33.0	37.0	22.0	37.0
94-95	33.65693598060415	37.0	33.0	37.0	22.0	37.0
96-97	33.571539822473575	37.0	33.0	37.0	22.0	37.0
98-99	33.477231219515474	37.0	33.0	37.0	22.0	37.0
100-101	33.38902330223345	37.0	33.0	37.0	22.0	37.0
102-103	33.26913415963937	37.0	33.0	37.0	14.0	37.0
104-105	33.14059803857327	37.0	33.0	37.0	14.0	37.0
106-107	33.05504982534666	37.0	33.0	37.0	14.0	37.0
108-109	32.93447349452854	37.0	33.0	37.0	14.0	37.0
110-111	32.8673600715079	37.0	33.0	37.0	14.0	37.0
112-113	32.72773989001628	37.0	33.0	37.0	14.0	37.0
114-115	32.621131625947825	37.0	33.0	37.0	14.0	37.0
116-117	32.42278037363549	37.0	33.0	37.0	14.0	37.0
118-119	32.34906977564732	37.0	33.0	37.0	14.0	37.0
120-121	32.25560965692674	37.0	33.0	37.0	14.0	37.0
122-123	32.16194707810591	37.0	33.0	37.0	14.0	37.0
124-125	30.56524171719768	35.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	1137.0
1	64.0
2	50.0
3	21.0
4	21.0
5	33.0
6	31.0
7	28.0
8	20.0
9	25.0
10	19.0
11	21.0
12	23.0
13	24.0
14	52.0
15	204.0
16	322.0
17	662.0
18	1328.0
19	2534.0
20	3935.0
21	4699.0
22	5268.0
23	5662.0
24	6194.0
25	6942.0
26	8223.0
27	9715.0
28	11406.0
29	13648.0
30	16568.0
31	19971.0
32	25244.0
33	33381.0
34	50852.0
35	94027.0
36	272825.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.698043184885286	19.548751686909583	12.219298245614036	24.533906882591094
2	17.599268847085167	22.570805145050215	35.68822128842094	24.141704719443677
3	19.87901125080718	26.71919178976013	28.192266903608587	25.209530055824104
4	14.737883784130068	18.881032820160087	33.94276870789585	32.43831468781399
5	15.805111826473778	36.7925371448036	31.191180016761745	16.21117101196087
6	30.062244928908676	35.495448739767674	18.27244204346698	16.16986428785668
7	26.557603003322793	32.60704729424611	21.69405333998342	19.141296362447683
8	25.59700624933657	33.57472498032866	20.43578549729319	20.392483273041584
9	26.38324402655613	18.008290364978265	19.87126343814242	35.73720217032319
10-11	25.247928905843885	26.80277656784334	25.65949383832112	22.289800687991658
12-13	26.02388862761756	25.61765385088655	25.24774421705411	23.11071330444178
14-15	24.478260136274706	25.89143560682723	23.353150509343585	26.277153747554475
16-17	24.26174867211281	27.74515018760279	24.128981027292813	23.86412011299159
18-19	24.263920716244016	26.864038876616803	25.414846572717092	23.45719383442209
20-21	25.81188571871595	25.83640851438207	24.902351238906807	23.449354527995173
22-23	25.510565219882636	25.72738058723005	24.73667002605492	24.02538416683239
24-25	24.64563028268694	26.319576441406827	24.818433965570698	24.21635931033553
26-27	24.47161321851671	26.27963802430023	25.001348140408826	24.247400616774236
28-29	24.29611431681448	26.145902049543913	25.319748902015	24.2382347316266
30-31	24.560689584865663	26.089945632255667	25.246499965877984	24.102864817000683
32-33	24.17235420838771	26.260561724957476	24.993196854798555	24.57388721185626
34-35	24.50720242608036	26.22373852244967	25.057872125347487	24.211186926122483
36-37	24.467442536360995	26.357835146644426	24.77720071942749	24.39752159756709
38-39	24.460342570644457	26.22415852526488	24.867960193473063	24.4475387106176
40-41	24.279831577386616	26.420412901616995	25.076620831767784	24.2231346892286
42-43	24.361000429622017	26.216462104810923	25.19682585145187	24.225711614115188
44-45	24.318906037967572	26.41464456257776	25.020237185712922	24.246212213741742
46-47	24.266690925692412	26.27530456448731	25.06429062986707	24.3937138799532
48-49	24.3732960044067	26.506570042694115	24.96245629727706	24.157677655622123
50-51	23.99737203406248	26.709567649065473	24.918086638646262	24.374973678225786
52-53	24.080082477978078	26.35024839415579	25.028427611928983	24.541241515937152
54-55	24.158454705266465	26.228298563584122	25.10851244660716	24.504734284542252
56-57	24.21247954287216	26.088344469816104	24.850682971162506	24.848493016149234
58-59	24.19764815273363	26.06100114375039	25.13294624373169	24.60840445978429
60-61	24.037525392552865	26.284871597061034	25.206593473229592	24.471009537156505
62-63	23.8452099109183	26.302810406817944	25.175413551502228	24.676566130761525
64-65	23.996176471826743	25.955439895231958	25.151825229390635	24.89655840355066
66-67	24.12916070470587	25.93842456010625	25.060700410733126	24.87171432445475
68-69	24.276785285643175	25.749282196709682	25.025288405739733	24.948644111907406
70-71	23.88255546796569	25.78982475289552	25.295110464293657	25.032509314845136
72-73	24.218760526563994	25.849030545562336	25.211289192503067	24.720919735370604
74-75	24.113159776530665	25.75717376821079	25.380850467077188	24.74881598818136
76-77	24.061241681408475	25.602982057113977	25.454974307135036	24.880801954342516
78-79	24.0694431693469	25.647429024327177	25.419090638800007	24.864037167525918
80-81	23.927719794173413	25.767574984438347	25.503426933498872	24.801278287889367
82-83	24.065933603143385	25.7484702106961	25.445586668407376	24.740009517753137
84-85	23.99714969412276	25.80815521027323	25.314742331169494	24.879952764434517
86-87	23.81987080690939	25.80038235764757	25.471841127870842	24.907905707572198
88-89	24.00591160383833	25.512696895565877	25.38452793485446	25.09686356574133
90-91	24.2651516682497	25.596922175292526	25.321743494398753	24.816182662059024
92-93	24.05772986136783	25.82155750039367	25.328517739747426	24.792194898491072
94-95	23.49047651524911	25.96933590312914	25.558101213916977	24.982086367704774
96-97	23.75565477518559	25.936821437502154	25.289190233667565	25.018333553644695
98-99	23.667313848283342	26.05442050864097	25.308028700512075	24.970236942563613
100-101	23.873686160114833	26.383041415684836	24.983520438444735	24.7597519857556
102-103	23.90709899461358	26.31322392115858	25.351784219928913	24.42789286429893
104-105	23.638448598414115	26.416446217393574	25.317371178201032	24.627734005991282
106-107	23.713391138018157	26.385910676355223	25.34810358370524	24.552594601921378
108-109	23.774632993962722	26.402208476772348	25.157288301690457	24.665870227574473
110-111	23.529362240946163	26.745940339320956	25.059185845237852	24.665511574495024
112-113	23.722086957693072	26.708542544342635	25.046641005694582	24.522729492269715
114-115	23.564534295445398	26.764606352835408	25.152069713841247	24.518789637877944
116-117	23.689348014038654	26.75443211885546	25.06341219484065	24.492807672265236
118-119	23.420424834125456	26.614341055906582	25.197441634552415	24.767792475415547
120-121	23.4411328461058	26.568126834048012	25.302749451574396	24.68799086827179
122-123	23.570106469210288	26.726978737301593	25.263395348484863	24.439519445003253
124-125	23.405843885283893	26.61667274124566	25.149851306090596	24.82763206737985
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1686.0
1	967.5
2	211.5
3	161.5
4	139.0
5	115.0
6	95.5
7	87.5
8	92.5
9	91.5
10	82.5
11	81.0
12	79.5
13	88.0
14	95.5
15	106.0
16	132.0
17	151.5
18	189.5
19	244.0
20	300.5
21	376.5
22	471.5
23	600.5
24	765.5
25	1000.5
26	1317.0
27	1683.0
28	2114.5
29	2870.0
30	4034.5
31	5160.5
32	6326.5
33	7409.0
34	8542.0
35	9507.0
36	10429.0
37	11801.5
38	12523.0
39	12755.5
40	13099.5
41	13594.5
42	14289.0
43	15542.5
44	17119.0
45	18521.0
46	20435.5
47	22228.5
48	23821.5
49	25135.0
50	25343.5
51	24794.5
52	24379.5
53	24458.0
54	24046.5
55	23280.5
56	22335.5
57	20770.5
58	19423.0
59	17750.0
60	15430.0
61	12928.5
62	10513.5
63	8253.5
64	6314.0
65	5060.0
66	4037.0
67	3135.0
68	2554.0
69	2179.0
70	1829.5
71	1580.0
72	1411.0
73	1348.0
74	1116.5
75	917.5
76	715.5
77	486.5
78	363.0
79	218.0
80	125.0
81	87.5
82	70.5
83	38.5
84	15.5
85	12.5
86	9.5
87	7.0
88	3.0
89	1.0
90	1.5
91	1.0
92	0.5
93	1.0
94	1.0
95	1.0
96	1.5
97	1.0
98	0.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.39971168337592555
2	0.36005974673165553
3	0.34712246231805893
4	0.3372094781569914
5	0.3632520636648806
6	0.28848464075513414
7	0.28613240722538935
8	0.2815959568465957
9	0.2888206741165263
10-11	0.2877285656920019
12-13	0.32360012702061064
14-15	0.37954968169239844
16-17	0.34233398691822126
18-19	0.3221719852346941
20-21	0.3114189176701463
22-23	0.3052863088247401
24-25	0.290836874284879
26-27	0.29722150815132925
28-29	0.2865524489271295
30-31	0.2890726991375704
32-33	0.28621641556573735
34-35	0.27369917285388096
36-37	0.2774795481695423
38-39	0.27017082255926367
40-41	0.28092389012381147
42-43	0.27453925625736125
44-45	0.26773458068917083
46-47	0.26639044724360234
48-49	0.25832564657019147
50-51	0.26277808860863705
52-53	0.2631141219700292
54-55	0.26605441388221024
56-57	0.2620220135455048
58-59	0.25504932129661834
60-61	0.2521930377247853
62-63	0.25378919619139784
64-65	0.24975679585469246
66-67	0.24942076249330034
68-69	0.2566454797632309
70-71	0.25252907108617745
72-73	0.24244807024441387
74-75	0.25882969661227967
76-77	0.27369917285388096
78-79	0.25916572997367177
80-81	0.26420623039455354
82-83	0.2606778800999363
84-85	0.26185399686480876
86-87	0.2510169209599129
88-89	0.24051587841640917
90-91	0.23236706940265028
92-93	0.2374915781638801
94-95	0.22808264404490078
96-97	0.2222020602205387
98-99	0.22211805188019065
100-101	0.21195304269807907
102-103	0.21472531792956404
104-105	0.22110995179601428
106-107	0.21548139299269634
108-109	0.2159854430347845
110-111	0.21623746805582858
112-113	0.2152293679716522
114-115	0.20296415028083986
116-117	0.20960080916833423
118-119	0.20262811691944774
120-121	0.20262811691944774
122-123	0.19447930790568888
124-125	0.19716757479682584
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	595179.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.44027859796141
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.23641109626871	69.30084801373879
2	8.995876556905529	14.292698798268901
3	2.0187807121131995	4.811175065953905
4	0.6892887824907482	2.1902917166205866
5	0.3356455921103589	1.3331889673712314
6	0.19363773676918022	0.9229581453613436
7	0.11213347243969629	0.6235540003536411
8	0.08059278595763607	0.5121850695568386
9	0.05560705953097147	0.3975696271038419
>10	0.26231517644928826	3.683111793625298
>50	0.013898803793409116	0.8074163213231754
>100	0.005306814286789628	0.6955984289265373
>500	2.5270544222777983E-4	0.17304642012532043
>1k	2.5270544222777983E-4	0.2563576316705733
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	1277	0.214557301248868	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	862	0.1448303787600033	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	3.36033361392119E-4	0.0	0.0	0.0
6	0.0	3.36033361392119E-4	0.0	0.0	0.0
7	0.0	3.36033361392119E-4	0.0	0.0	0.0
8	0.0	3.36033361392119E-4	0.0	0.0	1.680166806960595E-4
9	0.0	3.36033361392119E-4	0.0	0.0	1.680166806960595E-4
10-11	0.0	3.36033361392119E-4	0.0	0.0	1.680166806960595E-4
12-13	0.0	3.36033361392119E-4	0.0	0.0	2.5202502104408923E-4
14-15	0.0	3.36033361392119E-4	0.0	1.680166806960595E-4	3.36033361392119E-4
16-17	0.0	3.36033361392119E-4	0.0	3.36033361392119E-4	3.36033361392119E-4
18-19	0.0	3.36033361392119E-4	0.0	4.2004170174014875E-4	3.36033361392119E-4
20-21	0.0	3.36033361392119E-4	0.0	0.001008100084176357	3.36033361392119E-4
22-23	0.0	3.36033361392119E-4	0.0	0.002772275231484982	3.36033361392119E-4
24-25	0.0	3.36033361392119E-4	0.0	0.00714070892958253	3.36033361392119E-4
26-27	0.0	3.36033361392119E-4	0.0	0.012769267732900522	3.36033361392119E-4
28-29	0.0	3.36033361392119E-4	0.0	0.025874568827193165	3.36033361392119E-4
30-31	0.0	3.36033361392119E-4	0.0	0.04796876233872499	3.36033361392119E-4
32-33	0.0	3.36033361392119E-4	0.0	0.08064800673410856	3.36033361392119E-4
34-35	0.0	3.36033361392119E-4	0.0	0.11702361810480544	3.36033361392119E-4
36-37	0.0	3.36033361392119E-4	0.0	0.16751263065397132	3.36033361392119E-4
38-39	0.0	3.36033361392119E-4	0.0	0.24799262070738384	3.36033361392119E-4
40-41	0.0	3.36033361392119E-4	0.0	0.3265404189327916	3.36033361392119E-4
42-43	0.0	3.36033361392119E-4	0.0	0.39945965835488145	3.36033361392119E-4
44-45	0.0	3.36033361392119E-4	0.0	0.47363902288219173	3.36033361392119E-4
46-47	0.0	3.36033361392119E-4	0.0	0.5713407227069502	3.36033361392119E-4
48-49	0.0	3.36033361392119E-4	0.0	0.6653460555563957	3.36033361392119E-4
50-51	0.0	3.36033361392119E-4	0.0	0.7533027879007828	3.36033361392119E-4
52-53	0.0	3.36033361392119E-4	0.0	0.8364710448453323	3.36033361392119E-4
54-55	0.0	3.36033361392119E-4	0.0	0.9171190515794408	3.36033361392119E-4
56-57	0.0	3.36033361392119E-4	0.0	1.0033116087765193	3.36033361392119E-4
58-59	0.0	5.040500420881786E-4	0.0	1.1021894253661504	5.040500420881785E-4
60-61	0.0	5.040500420881786E-4	0.0	1.2097201010116285	6.72066722784238E-4
62-63	0.0	5.040500420881786E-4	0.0	1.3021292753944613	6.72066722784238E-4
64-65	0.0	5.040500420881786E-4	0.0	1.389329932675716	6.72066722784238E-4
66-67	0.0	5.040500420881786E-4	0.0	1.4942563497704051	7.560750631322678E-4
68-69	0.0	5.040500420881786E-4	0.0	1.6137162097453035	8.400834034802975E-4
70-71	0.0	5.040500420881786E-4	0.0	1.7347722281868143	8.400834034802975E-4
72-73	0.0	5.040500420881786E-4	0.0	1.8509557628881397	8.400834034802975E-4
74-75	0.0	5.040500420881786E-4	0.0	1.9608386720633626	8.400834034802975E-4
76-77	0.0	5.040500420881786E-4	0.0	2.0855070491398386	8.400834034802975E-4
78-79	0.0	5.040500420881786E-4	0.0	2.216980101784505	8.400834034802975E-4
80-81	0.0	5.040500420881786E-4	0.0	2.3648347807970374	8.400834034802975E-4
82-83	0.0	5.040500420881786E-4	0.0	2.5094971428763446	8.400834034802975E-4
84-85	0.0	5.040500420881786E-4	0.0	2.6444145374752805	8.400834034802975E-4
86-87	0.0	5.040500420881786E-4	0.0	2.7874807410879754	8.400834034802975E-4
88-89	0.0	5.040500420881786E-4	0.0	2.9445763375387908	8.400834034802975E-4
90-91	0.0	5.040500420881786E-4	0.0	3.1077205344946646	8.400834034802975E-4
92-93	0.0	5.040500420881786E-4	0.0	3.289262557986757	8.400834034802975E-4
94-95	0.0	5.040500420881786E-4	0.0	3.458959405489777	8.400834034802975E-4
96-97	0.0	5.040500420881786E-4	0.0	3.625211911038528	8.400834034802975E-4
98-99	0.0	5.040500420881786E-4	0.0	3.812382493333938	8.400834034802975E-4
100-101	0.0	5.040500420881786E-4	0.0	4.018118918846263	8.400834034802975E-4
102-103	0.0	5.040500420881786E-4	0.0	4.22461141942172	8.400834034802975E-4
104-105	0.0	5.040500420881786E-4	0.0	4.435976403737364	0.001008100084176357
106-107	0.0	5.040500420881786E-4	0.0	4.641544812568991	0.0011761167648724165
108-109	0.0	5.040500420881786E-4	0.0	4.860302530835261	0.0011761167648724165
110-111	0.0	5.040500420881786E-4	0.0	5.091829516834432	0.0011761167648724165
112-113	0.0	5.040500420881786E-4	0.0	5.344946646303045	0.0011761167648724165
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2965	0.0	28.940184	1
CACGATT	85	0.007176065	28.025162	2
TAACGCA	110	7.706243E-4	27.069756	4
GGTATCA	2165	0.0	25.872204	1
AACGCAG	3410	0.0	25.65314	6
ATCAACG	3425	0.0	25.212418	3
CAACGCA	3435	0.0	25.136898	5
TATCAAC	3600	0.0	24.483091	2
TCAACGC	3510	0.0	24.43219	4
CATGGGG	2255	0.0	24.032661	4
ACGCAGA	3715	0.0	23.236448	7
CGCAGAG	3815	0.0	22.941402	8
GCAGAGT	4055	0.0	21.3079	9
GTACATG	4890	0.0	21.081463	1
GCTTATG	170	3.8912223E-4	21.031286	1
ACATGGG	4820	0.0	21.004333	3
TACATGG	4995	0.0	20.626123	2
TCTAGAC	150	0.004678885	19.851156	3
ATGGGGA	825	0.0	19.48858	5
ATGGGGG	1115	0.0	19.226404	5
>>END_MODULE