FastQCFastQC Report
Sun 1 Jan 2017
ERR1142531_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142531_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences595179
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20970.35233097941963676No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19720.33132889433262935No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13830.23236706940265028No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23100.038.6305541
GTATCAA31850.033.80811
CAACGCA41100.026.6312265
ATCAACG40350.026.536533
TAACGCA900.00953623326.4382444
TATCAAC40550.026.4056452
TCAACGC41450.026.1193284
AACGCAG43400.025.4940226
GAGTACT21500.023.79242312-13
ACGCAGA48800.022.1853717
CGCAGAG49600.021.8257088
GTACTTT25050.020.53936814-15
GCAGAGT53250.020.4413789
AGTACTT22900.020.0001812-13
CAGAGTA52800.019.03844310-11
GTACCGC2201.0222549E-418.927386
AGAGTAC50300.018.27002510-11
ACTTTTT28200.018.0341516-17
GCATTAC1650.00816204318.027591
ACATGGG40950.017.141283
Produced by FastQC (version 0.11.5)