##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142531_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	595179
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.287787371530246	33.0	33.0	33.0	14.0	33.0
2	29.66528892988496	33.0	27.0	33.0	14.0	33.0
3	31.09413470569358	33.0	33.0	33.0	27.0	33.0
4	31.90797558381596	33.0	33.0	33.0	27.0	33.0
5	32.14881069392569	33.0	33.0	33.0	33.0	33.0
6	35.36282530129591	37.0	37.0	37.0	33.0	37.0
7	35.90943732893802	37.0	37.0	37.0	33.0	37.0
8	36.05829842786792	37.0	37.0	37.0	33.0	37.0
9	36.090307285707325	37.0	37.0	37.0	37.0	37.0
10-11	36.16501170236181	37.0	37.0	37.0	37.0	37.0
12-13	36.18263917241704	37.0	37.0	37.0	37.0	37.0
14-15	36.20415874888059	37.0	37.0	37.0	37.0	37.0
16-17	36.20948655782546	37.0	37.0	37.0	37.0	37.0
18-19	36.202996913533575	37.0	37.0	37.0	37.0	37.0
20-21	36.21340134648568	37.0	37.0	37.0	37.0	37.0
22-23	36.20798952920046	37.0	37.0	37.0	37.0	37.0
24-25	36.187692274088974	37.0	37.0	37.0	37.0	37.0
26-27	36.146229957710204	37.0	37.0	37.0	37.0	37.0
28-29	36.118513926062576	37.0	37.0	37.0	37.0	37.0
30-31	36.0561940189422	37.0	37.0	37.0	37.0	37.0
32-33	36.017572864634005	37.0	37.0	37.0	37.0	37.0
34-35	35.964293095018476	37.0	37.0	37.0	37.0	37.0
36-37	35.90920294566845	37.0	37.0	37.0	35.0	37.0
38-39	35.85635917933932	37.0	37.0	37.0	33.0	37.0
40-41	35.72752062824797	37.0	37.0	37.0	33.0	37.0
42-43	35.69535635497893	37.0	37.0	37.0	33.0	37.0
44-45	35.625861295509424	37.0	37.0	37.0	33.0	37.0
46-47	35.50218841726607	37.0	37.0	37.0	33.0	37.0
48-49	35.38366525028604	37.0	37.0	37.0	33.0	37.0
50-51	35.30924982232236	37.0	37.0	37.0	33.0	37.0
52-53	35.24062424917546	37.0	37.0	37.0	33.0	37.0
54-55	35.19051411424127	37.0	37.0	37.0	33.0	37.0
56-57	35.13995789501982	37.0	37.0	37.0	33.0	37.0
58-59	35.10346467197263	37.0	37.0	37.0	33.0	37.0
60-61	35.06321291577828	37.0	37.0	37.0	33.0	37.0
62-63	35.053876228831996	37.0	37.0	37.0	33.0	37.0
64-65	35.02143556812321	37.0	37.0	37.0	33.0	37.0
66-67	34.97891642682285	37.0	37.0	37.0	27.0	37.0
68-69	34.9411513175028	37.0	37.0	37.0	27.0	37.0
70-71	34.9055712651152	37.0	37.0	37.0	27.0	37.0
72-73	34.882735277958396	37.0	37.0	37.0	27.0	37.0
74-75	34.786433157083835	37.0	37.0	37.0	27.0	37.0
76-77	34.74332007681723	37.0	37.0	37.0	27.0	37.0
78-79	34.71935333739934	37.0	37.0	37.0	27.0	37.0
80-81	34.68199314827976	37.0	37.0	37.0	27.0	37.0
82-83	34.642648682161166	37.0	37.0	37.0	27.0	37.0
84-85	34.56709662135257	37.0	37.0	37.0	27.0	37.0
86-87	34.402120202493705	37.0	37.0	37.0	27.0	37.0
88-89	34.32880024328816	37.0	37.0	37.0	27.0	37.0
90-91	34.290104321557045	37.0	37.0	37.0	27.0	37.0
92-93	34.23241915457366	37.0	37.0	37.0	27.0	37.0
94-95	34.23831485989929	37.0	37.0	37.0	27.0	37.0
96-97	34.20515928821413	37.0	37.0	37.0	27.0	37.0
98-99	34.129437530558036	37.0	37.0	37.0	27.0	37.0
100-101	34.07686595125164	37.0	37.0	37.0	27.0	37.0
102-103	33.98005221958436	37.0	37.0	37.0	22.0	37.0
104-105	33.88386182980246	37.0	37.0	37.0	22.0	37.0
106-107	33.8217637047006	37.0	35.0	37.0	22.0	37.0
108-109	33.72843127865735	37.0	33.0	37.0	22.0	37.0
110-111	33.649260978629954	37.0	33.0	37.0	22.0	37.0
112-113	33.602212947701446	37.0	33.0	37.0	22.0	37.0
114-115	33.4694402860316	37.0	33.0	37.0	22.0	37.0
116-117	33.35467985261577	37.0	33.0	37.0	22.0	37.0
118-119	33.21659702375252	37.0	33.0	37.0	14.0	37.0
120-121	33.12512454236456	37.0	33.0	37.0	14.0	37.0
122-123	32.993134838426755	37.0	33.0	37.0	14.0	37.0
124-125	31.703222055885707	37.0	30.0	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	59.0
16	20.0
17	15.0
18	61.0
19	329.0
20	1113.0
21	2731.0
22	4669.0
23	6496.0
24	5625.0
25	4174.0
26	4013.0
27	4769.0
28	6311.0
29	8187.0
30	11083.0
31	15229.0
32	21708.0
33	32103.0
34	53163.0
35	106925.0
36	306394.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.39856341446199	19.614232751057944	12.435842708336835	24.551361126143235
2	17.650488340482443	23.081795560663263	34.80801573980265	24.45970035905165
3	21.52022514386525	25.495526525811734	28.341748225311886	24.64250010501113
4	15.903813649026006	17.738222850979035	33.882468774047425	32.47549472594753
5	16.626229904833593	35.39454406150868	31.47733077047153	16.501895263186192
6	30.747969911168386	34.34088690477191	18.172256376738787	16.73888680732092
7	26.482552815392708	31.35754840465841	22.655211639633766	19.50468714031512
8	26.173806535512846	31.66660786082842	21.268895575952783	20.89069002770595
9	26.51857676430116	15.98174666780918	20.813402354585765	36.686274213303896
10-11	25.676225135631466	25.014575447050387	26.696254404137242	22.61294501318091
12-13	26.72112087288026	23.818380688834786	26.546635549977402	22.913862888307552
14-15	24.757593933925758	24.2805105690893	25.09009894502326	25.87179655196168
16-17	23.9137301551298	26.175486702319805	26.01629089736029	23.8944922451901
18-19	23.769487834752233	24.928382889853303	28.209412630485954	23.092716644908506
20-21	24.963855381200766	24.05496838343455	27.92027937837136	23.06089685699332
22-23	24.616711947162116	24.08351101097317	27.827930756965554	23.471846284899165
24-25	23.911461929940405	24.659892234101	27.95957182629091	23.46907400966768
26-27	23.769991884794322	24.750621241676875	27.88724064525126	23.592146228277542
28-29	23.534936548500536	24.54194452425237	28.38885444546935	23.53426448177775
30-31	23.94111687408326	24.379724419040322	28.247300391982915	23.431858314893503
32-33	23.58298931917961	24.485742944559536	28.031566973969174	23.89970076229168
34-35	23.928095581329316	24.43189359839645	28.078779661244766	23.561231159029468
36-37	23.9057694456369	24.602535205824807	27.730756403331103	23.760938945207194
38-39	23.800402904000308	24.450375433273017	27.82751071526381	23.921710947462866
40-41	23.61334788390792	24.689099082524105	27.94197580336898	23.755577230198995
42-43	23.814116269320884	24.56977192556519	27.739997328532535	23.876114476581396
44-45	23.821404989087316	24.725838781274202	27.39007928707162	24.062676942566856
46-47	23.89676508764634	24.696986881403348	27.266181912887145	24.14006611806317
48-49	23.922084250499893	24.86507149698385	27.212793843361954	24.0000504091543
50-51	23.71639456365228	25.044818449575672	26.96247683470015	24.276310152071897
52-53	23.850135841486342	24.927794831470866	26.839908666132374	24.382160660910415
54-55	23.891299928256878	25.02112809759753	26.812941988880656	24.274629985264937
56-57	23.911125896579012	24.863612459444973	26.617202555869746	24.60805908810627
58-59	23.90692547956161	24.86546064293263	26.791183828730514	24.436430048775243
60-61	23.818800730536527	24.959297959101377	27.07622412753138	24.14567718283071
62-63	23.550814124826314	25.008022796503237	26.984150986509942	24.45701209216051
64-65	23.574588485144805	24.726762873018032	27.15645209256375	24.542196549273413
66-67	23.881017092975874	24.761836749268014	26.98741026082646	24.36973589692965
68-69	23.900708862375858	24.663588601076313	27.03035557370136	24.405346962846473
70-71	23.587861802919793	24.59293758684362	27.436619907624426	24.382580702612156
72-73	23.795782445281166	24.736927882200142	27.41360162236907	24.053688050149617
74-75	23.82347403001335	24.713265927148868	27.434675740834763	24.02858430200302
76-77	23.771952086509163	24.68180084001553	27.419330441403115	24.12691663207219
78-79	23.807939648256195	24.84368108673463	27.308071961725705	24.040307303283473
80-81	23.54031308228281	24.941572199287947	27.42057431461796	24.09754040381129
82-83	23.65960492557701	24.885286611254763	27.413853647390113	24.04125481577811
84-85	23.719449307936518	24.87915051067367	27.17678277687793	24.224617404511882
86-87	23.55976090756164	24.90232065752945	27.273301035825998	24.264617399082912
88-89	23.772963018451215	24.658742570107382	27.118455760681286	24.449838650760118
90-91	23.849410743543075	24.825056604281404	27.058612766157875	24.26691988601765
92-93	23.655091165793678	25.040047695649285	26.953255404652012	24.35160573390503
94-95	23.257644555372885	24.94402939622315	27.230822349933675	24.56750369847029
96-97	23.468401942944894	24.877053793900657	26.925261139925972	24.729283123228473
98-99	23.484615552632064	25.025244506274586	26.748003541791633	24.742136399301724
100-101	23.632470231644596	25.233165148635955	26.489425870200396	24.644938749519053
102-103	23.731180031553535	25.20132598764405	26.582927153007752	24.484566827794662
104-105	23.54703888368498	25.244109900911084	26.555410406226766	24.65344080917717
106-107	23.666975262488467	25.29634439965791	26.469204545263615	24.567475792590006
108-109	23.720131931131007	25.159663216048152	26.43780905606737	24.68239579675347
110-111	23.606991191703315	25.476349492378326	26.241919427397708	24.67473988852065
112-113	23.58702171951631	25.49518716218146	26.22488360644445	24.69290751185778
114-115	23.619468781214376	25.455892643971517	26.35343850626325	24.571200068550862
116-117	23.66061302566119	25.482585911129256	26.258990992625748	24.597810070583808
118-119	23.398759028796377	25.455367208856494	26.392984295480854	24.75288946686627
120-121	23.334862292834387	25.377871419546487	26.564915251959086	24.72235103566004
122-123	23.421189255669304	25.691598661915155	26.337538790851156	24.549673291564385
124-125	23.382629427449558	25.549540558386635	26.347619791692917	24.720210222470886
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	162.0
1	143.5
2	211.5
3	337.5
4	282.5
5	230.0
6	364.5
7	699.0
8	1013.0
9	1131.0
10	1093.0
11	951.0
12	898.0
13	863.5
14	856.0
15	862.0
16	878.5
17	891.5
18	849.5
19	836.0
20	846.5
21	886.5
22	932.5
23	1013.0
24	1079.0
25	1180.0
26	1383.5
27	1690.5
28	2092.5
29	2761.5
30	3771.5
31	4636.5
32	5760.0
33	6934.5
34	7785.5
35	8808.5
36	9928.0
37	11067.5
38	11865.5
39	12253.5
40	12639.0
41	13207.5
42	13902.0
43	15162.0
44	16812.0
45	18127.0
46	19892.0
47	21665.5
48	23448.0
49	24900.5
50	25245.5
51	24681.0
52	24059.5
53	24198.0
54	23928.0
55	23244.0
56	22319.0
57	20682.0
58	19322.5
59	17719.5
60	15418.0
61	12977.5
62	10559.0
63	8273.5
64	6302.0
65	5008.5
66	3897.0
67	2942.5
68	2436.0
69	2075.0
70	1718.5
71	1506.0
72	1351.0
73	1309.0
74	1074.5
75	884.5
76	735.5
77	521.0
78	354.5
79	208.5
80	138.0
81	79.5
82	48.0
83	33.0
84	16.0
85	5.5
86	1.0
87	1.5
88	1.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.026378618869281344
2	0.0
3	6.72066722784238E-4
4	1.680166806960595E-4
5	0.0018481834876566546
6	0.001344133445568476
7	0.00672066722784238
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	8.400834034802975E-5
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	8.400834034802975E-5
38-39	0.0
40-41	0.0022682251893968034
42-43	8.400834034802975E-5
44-45	0.0
46-47	0.0052925254419258745
48-49	0.008232817354106916
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.008652859055847065
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.06334228862241444
76-77	0.031923169332251304
78-79	2.520250210440893E-4
80-81	0.0
82-83	0.0
84-85	0.05502546292795949
86-87	0.17062093924684843
88-89	0.189186782463763
90-91	0.11777969316793771
92-93	0.09669359974058225
94-95	8.400834034802975E-5
96-97	0.0
98-99	0.0
100-101	0.0
102-103	0.0
104-105	0.0010921084245243868
106-107	0.001680166806960595
108-109	0.00336033361392119
110-111	2.520250210440893E-4
112-113	0.0
114-115	8.400834034802975E-5
116-117	0.0
118-119	0.0
120-121	5.040500420881786E-4
122-123	0.0
124-125	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	595179.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.97355582228158
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.4943400062497	64.95309012953932
2	10.206233770419077	15.508077421843783
3	2.248066326021962	5.12380777536663
4	0.7958898799577995	2.4186633689345145
5	0.38042669871933377	1.4451184515719802
6	0.23455068676043184	1.0691789816248891
7	0.14130392605401204	0.7514753199780423
8	0.09407417886088491	0.5717719903298194
9	0.06613184104488731	0.4521844005528598
>10	0.3089373469047598	4.129334246851633
>50	0.020207486265420807	1.0884004541036223
>100	0.00877430109397773	1.2714163305619046
>500	2.658879119386753E-4	0.11615258819082135
>1k	7.97663735816026E-4	1.1013285405501876
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2097	0.35233097941963676	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1972	0.33132889433262935	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1383	0.23236706940265028	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	1.680166806960595E-4
6	0.0	0.0	0.0	0.0	1.680166806960595E-4
7	0.0	0.0	0.0	0.0	1.680166806960595E-4
8	0.0	0.0	0.0	0.0	1.680166806960595E-4
9	0.0	0.0	0.0	0.0	1.680166806960595E-4
10-11	0.0	0.0	0.0	0.0	1.680166806960595E-4
12-13	0.0	0.0	0.0	0.0	1.680166806960595E-4
14-15	0.0	0.0	0.0	1.680166806960595E-4	1.680166806960595E-4
16-17	0.0	0.0	0.0	3.36033361392119E-4	1.680166806960595E-4
18-19	0.0	0.0	0.0	4.2004170174014875E-4	1.680166806960595E-4
20-21	0.0	0.0	0.0	0.001008100084176357	1.680166806960595E-4
22-23	0.0	0.0	0.0	0.002772275231484982	1.680166806960595E-4
24-25	0.0	0.0	0.0	0.007308725610278588	1.680166806960595E-4
26-27	0.0	0.0	0.0	0.01318930943464067	1.680166806960595E-4
28-29	0.0	0.0	0.0	0.02663064389032543	1.680166806960595E-4
30-31	0.0	0.0	0.0	0.04889285408255331	1.680166806960595E-4
32-33	0.0	0.0	0.0	0.08140408179724082	1.680166806960595E-4
34-35	1.680166806960595E-4	0.0	0.0	0.11786370150828573	1.680166806960595E-4
36-37	1.680166806960595E-4	0.0	0.0	0.16810068903640754	1.680166806960595E-4
38-39	1.680166806960595E-4	0.0	0.0	0.24874869577051612	2.5202502104408923E-4
40-41	1.680166806960595E-4	0.0	0.0	0.3273805023362719	3.36033361392119E-4
42-43	1.680166806960595E-4	0.0	0.0	0.40071978346010195	4.2004170174014875E-4
44-45	1.680166806960595E-4	0.0	0.0	0.4758232397312405	5.040500420881786E-4
46-47	1.680166806960595E-4	0.0	0.0	0.5738609729173912	5.040500420881786E-4
48-49	1.680166806960595E-4	0.0	0.0	0.6676142807457924	5.040500420881786E-4
50-51	1.680166806960595E-4	0.0	0.0	0.7558230381112236	6.72066722784238E-4
52-53	1.680166806960595E-4	0.0	0.0	0.838739270034729	6.72066722784238E-4
54-55	1.680166806960595E-4	0.0	0.0	0.9199753351512738	6.72066722784238E-4
56-57	1.680166806960595E-4	0.0	0.0	1.0066719423904404	6.72066722784238E-4
58-59	1.680166806960595E-4	0.0	0.0	1.1060538090221597	6.72066722784238E-4
60-61	1.680166806960595E-4	0.0	0.0	1.214256551390422	6.72066722784238E-4
62-63	1.680166806960595E-4	0.0	0.0	1.306833742453951	9.240917438283273E-4
64-65	1.680166806960595E-4	0.0	0.0	1.3941184080755535	0.0010081000841763571
66-67	1.680166806960595E-4	0.0	0.0	1.499632883552679	0.0010081000841763571
68-69	1.680166806960595E-4	0.0	0.0	1.6192607602082734	0.0010081000841763571
70-71	1.680166806960595E-4	0.0	0.0	1.7404847953304805	0.0010081000841763571
72-73	1.680166806960595E-4	0.0	0.0	1.8565843216914575	0.001344133445568476
74-75	1.680166806960595E-4	0.0	0.0	1.9662152058456364	0.001344133445568476
76-77	1.680166806960595E-4	0.0	0.0	2.092227716367681	0.001344133445568476
78-79	1.680166806960595E-4	0.0	0.0	2.222692668928171	0.001344133445568476
80-81	1.680166806960595E-4	0.0	0.0	2.369623256196875	0.001344133445568476
82-83	1.680166806960595E-4	0.0	0.0	2.5137815682340943	0.001344133445568476
84-85	1.680166806960595E-4	0.0	0.0	2.6504631379803385	0.001344133445568476
86-87	1.680166806960595E-4	0.0	0.0	2.7944534333368614	0.001344133445568476
88-89	1.680166806960595E-4	0.0	0.0	2.9531451882542896	0.001344133445568476
90-91	1.680166806960595E-4	0.0	0.0	3.117213476953992	0.001344133445568476
92-93	1.680166806960595E-4	0.0	0.0	3.300855708954785	0.001344133445568476
94-95	1.680166806960595E-4	0.0	0.0	3.473408840029638	0.001344133445568476
96-97	1.680166806960595E-4	0.0	0.0	3.6408374623432613	0.001344133445568476
98-99	1.680166806960595E-4	0.0	0.0	3.828680111361456	0.001344133445568476
100-101	1.680166806960595E-4	0.0	0.0	4.037692862147354	0.001680166806960595
102-103	1.680166806960595E-4	0.0	0.0	4.248469788080561	0.0018481834876566546
104-105	1.680166806960595E-4	0.0	0.0	4.4620189892452515	0.0018481834876566546
106-107	1.680166806960595E-4	0.0	0.0	4.669099548203146	0.0018481834876566546
108-109	1.680166806960595E-4	0.0	0.0	4.890125491658812	0.0018481834876566546
110-111	1.680166806960595E-4	0.0	0.0	5.12383669450703	0.0018481834876566546
112-113	1.680166806960595E-4	0.0	0.0	5.379138040824693	0.0018481834876566546
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2310	0.0	38.630554	1
GTATCAA	3185	0.0	33.8081	1
CAACGCA	4110	0.0	26.631226	5
ATCAACG	4035	0.0	26.53653	3
TAACGCA	90	0.009536233	26.438244	4
TATCAAC	4055	0.0	26.405645	2
TCAACGC	4145	0.0	26.119328	4
AACGCAG	4340	0.0	25.494022	6
GAGTACT	2150	0.0	23.792423	12-13
ACGCAGA	4880	0.0	22.185371	7
CGCAGAG	4960	0.0	21.825708	8
GTACTTT	2505	0.0	20.539368	14-15
GCAGAGT	5325	0.0	20.441378	9
AGTACTT	2290	0.0	20.00018	12-13
CAGAGTA	5280	0.0	19.038443	10-11
GTACCGC	220	1.0222549E-4	18.92738	6
AGAGTAC	5030	0.0	18.270025	10-11
ACTTTTT	2820	0.0	18.03415	16-17
GCATTAC	165	0.008162043	18.02759	1
ACATGGG	4095	0.0	17.14128	3
>>END_MODULE