FastQCFastQC Report
Sun 1 Jan 2017
ERR1142522_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142522_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences516166
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12300.23829543208967657No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11540.22357148669226568No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA7040.13639023104970108No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT5220.10113025654537493No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGAC900.0095083226.4534763
CATGGGT8200.025.4050164
GTTAAGC1651.1053469E-525.2510453
GGTATCA31750.024.1971741
TTAGACT1552.0992057E-423.0401254
ATGGGTG5750.022.7729935
CATGGGG22800.022.450654
CCGTCGA1653.18772E-421.66069
ATACTAG1703.930814E-420.9990046
ACATGGG54550.020.9494083
GTACATG57450.020.8364981
TACATGG57450.020.4079422
ATGGGGG12900.020.3015065
GTATCAA38450.020.290541
ATGGGGC5700.019.8401075
TAGCCCG2158.528E-519.378715
GTGCTAG3202.6926136E-718.6109581
TATCAAC42350.018.5499842
AACGCAG42100.018.2307266
ATCAACG41700.018.1272933
Produced by FastQC (version 0.11.5)