##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1142522_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 516166 Sequences flagged as poor quality 0 Sequence length 125 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.09123615271056 33.0 33.0 33.0 27.0 33.0 2 31.181325387569114 33.0 33.0 33.0 27.0 33.0 3 31.293568348167064 33.0 33.0 33.0 27.0 33.0 4 31.24006036817613 33.0 33.0 33.0 27.0 33.0 5 31.306786963883713 33.0 33.0 33.0 27.0 33.0 6 34.640596242294144 37.0 37.0 37.0 27.0 37.0 7 34.6310295525083 37.0 37.0 37.0 27.0 37.0 8 34.60693846553241 37.0 37.0 37.0 27.0 37.0 9 34.5182421934029 37.0 37.0 37.0 27.0 37.0 10-11 34.62145026987442 37.0 37.0 37.0 27.0 37.0 12-13 34.55939368342742 37.0 37.0 37.0 27.0 37.0 14-15 34.52654184894007 37.0 37.0 37.0 27.0 37.0 16-17 34.51376592026596 37.0 37.0 37.0 27.0 37.0 18-19 34.56142403800328 37.0 37.0 37.0 27.0 37.0 20-21 34.53653282083671 37.0 37.0 37.0 27.0 37.0 22-23 34.62787843445713 37.0 37.0 37.0 27.0 37.0 24-25 34.72126602682083 37.0 37.0 37.0 27.0 37.0 26-27 34.77932874307878 37.0 37.0 37.0 27.0 37.0 28-29 34.833766656463226 37.0 37.0 37.0 27.0 37.0 30-31 34.771681009597685 37.0 37.0 37.0 27.0 37.0 32-33 34.79778695225954 37.0 37.0 37.0 27.0 37.0 34-35 34.84957746151432 37.0 37.0 37.0 27.0 37.0 36-37 34.84135723778785 37.0 37.0 37.0 27.0 37.0 38-39 34.80767621269126 37.0 37.0 37.0 27.0 37.0 40-41 34.76789056233847 37.0 37.0 37.0 27.0 37.0 42-43 34.77716277321637 37.0 37.0 37.0 27.0 37.0 44-45 34.76261415901086 37.0 37.0 37.0 27.0 37.0 46-47 34.740923462606986 37.0 37.0 37.0 27.0 37.0 48-49 34.7373412816807 37.0 37.0 37.0 27.0 37.0 50-51 34.65930243371318 37.0 37.0 37.0 27.0 37.0 52-53 34.66989398759314 37.0 37.0 37.0 27.0 37.0 54-55 34.64246967060984 37.0 37.0 37.0 27.0 37.0 56-57 34.62003405881053 37.0 37.0 37.0 27.0 37.0 58-59 34.579151861997886 37.0 37.0 37.0 27.0 37.0 60-61 34.5652096418594 37.0 37.0 37.0 27.0 37.0 62-63 34.52906719931185 37.0 37.0 37.0 27.0 37.0 64-65 34.49529802427901 37.0 37.0 37.0 27.0 37.0 66-67 34.451354796712685 37.0 37.0 37.0 27.0 37.0 68-69 34.42901508429459 37.0 37.0 37.0 27.0 37.0 70-71 34.36470534672954 37.0 37.0 37.0 27.0 37.0 72-73 34.29926903360547 37.0 37.0 37.0 27.0 37.0 74-75 34.22804582246796 37.0 37.0 37.0 27.0 37.0 76-77 34.18844325275202 37.0 37.0 37.0 27.0 37.0 78-79 34.1385077668812 37.0 37.0 37.0 27.0 37.0 80-81 34.09384674697675 37.0 37.0 37.0 27.0 37.0 82-83 34.03827935199142 37.0 37.0 37.0 27.0 37.0 84-85 33.97241875675655 37.0 37.0 37.0 27.0 37.0 86-87 33.89863144802254 37.0 37.0 37.0 27.0 37.0 88-89 33.851285245444295 37.0 37.0 37.0 22.0 37.0 90-91 33.76132775114982 37.0 37.0 37.0 22.0 37.0 92-93 33.690206251477235 37.0 35.0 37.0 22.0 37.0 94-95 33.63657040564469 37.0 33.0 37.0 22.0 37.0 96-97 33.543527663581095 37.0 33.0 37.0 22.0 37.0 98-99 33.45498734903113 37.0 33.0 37.0 22.0 37.0 100-101 33.36199207231782 37.0 33.0 37.0 18.0 37.0 102-103 33.24998837583258 37.0 33.0 37.0 14.0 37.0 104-105 33.12458782639693 37.0 33.0 37.0 14.0 37.0 106-107 33.0359700173975 37.0 33.0 37.0 14.0 37.0 108-109 32.936236598303644 37.0 33.0 37.0 14.0 37.0 110-111 32.86639860045024 37.0 33.0 37.0 14.0 37.0 112-113 32.726779756899916 37.0 33.0 37.0 14.0 37.0 114-115 32.62571246459473 37.0 33.0 37.0 14.0 37.0 116-117 32.41889043447263 37.0 33.0 37.0 14.0 37.0 118-119 32.335508344214844 37.0 33.0 37.0 14.0 37.0 120-121 32.24024926089669 37.0 33.0 37.0 14.0 37.0 122-123 32.160210087452484 37.0 33.0 37.0 14.0 37.0 124-125 30.609296234157227 35.0 27.5 37.0 14.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 1039.0 1 51.0 2 37.0 3 14.0 4 19.0 5 25.0 6 16.0 7 24.0 8 31.0 9 27.0 10 14.0 11 21.0 12 15.0 13 25.0 14 64.0 15 266.0 16 407.0 17 684.0 18 1351.0 19 2635.0 20 3783.0 21 4743.0 22 4920.0 23 5043.0 24 5500.0 25 6132.0 26 6981.0 27 8154.0 28 9910.0 29 11744.0 30 14030.0 31 17115.0 32 21359.0 33 28297.0 34 42590.0 35 79570.0 36 239530.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.693928524187086 20.452617900855127 12.226207798068769 23.62724577688902 2 16.77879100153604 23.599968890357957 36.234566701016895 23.38667340708911 3 19.26287117451837 28.850561974698014 28.312403882981723 23.574162967801893 4 14.187288460155175 19.804114832721922 34.57307103850633 31.435525668616577 5 14.617490588930714 38.48232896742681 31.401471549015337 15.498708894627136 6 28.54874970610467 37.151284686935895 18.5718532796776 15.728112327281844 7 25.46143099471679 34.44872136154933 21.79582589298726 18.294021750746627 8 25.044590293887925 34.86028374581784 20.410502714276276 19.68462324601796 9 26.373756628561544 18.965312875877576 19.90385306696669 34.757077428594194 10-11 24.317512996860934 28.177077918986637 25.774346707963865 21.731062376188564 12-13 25.336155729395976 27.245355049455107 25.230510256941063 22.187978964207858 14-15 24.089367027312424 27.094910412248534 23.74194527013427 25.073777290304765 16-17 23.267049072127268 29.100141822462383 24.463062490340228 23.169746615070117 18-19 23.4692994945367 28.12342984206337 25.941220089857982 22.466050573541946 20-21 25.332783957179757 26.74000967886761 25.367573461244685 22.559632902707946 22-23 25.189919912002317 26.662345814571314 25.007530720481036 23.140203552945337 24-25 24.072474596097628 27.393077236218645 25.183163617744984 23.351284549938743 26-27 23.78059266873881 27.263364389545757 25.29981762693486 23.656225314780574 28-29 23.63886840589172 27.040567992701902 25.854140981959745 23.46642261944664 30-31 24.20821513232037 26.87288695449423 25.639062682159096 23.279835231026308 32-33 23.605954094946092 27.300255891627856 25.441592784516708 23.65219722890934 34-35 23.749612661382997 27.244256946110635 25.662846868409776 23.343283524096588 36-37 23.921657388042085 27.424188407725854 25.15758766770252 23.496566536529546 38-39 23.802469951257606 27.236972726407345 25.372658801314042 23.587898521021007 40-41 23.43661960884889 27.359011125229753 25.626692779675373 23.577676486245984 42-43 23.696247878764922 27.295572191931583 25.42383418279874 23.58434574650476 44-45 23.66736020856767 27.336527122927873 25.418213537083123 23.577899131421333 46-47 23.635168003232625 27.216698526669152 25.522678882257864 23.62545458784036 48-49 23.635941580799404 27.349156414233583 25.58865493452267 23.42624707044434 50-51 23.28269744644767 27.586407863181115 25.482682487831553 23.64821220253966 52-53 23.217093907507724 27.364686021448975 25.389577402130907 24.02864266891239 54-55 23.149662744976144 27.32338793652767 25.360567583109273 24.16638173538691 56-57 23.47938254480746 27.0607741890364 25.067410317081034 24.3924329490751 58-59 23.486858719089337 27.068804755337126 25.476407855630452 23.967928669943085 60-61 23.543203233808136 27.118593037056314 25.528106431292265 23.810097297843285 62-63 23.207219877195264 27.136796548569254 25.575902144712227 24.080081429523254 64-65 23.026718969584604 26.913600150731853 25.682202472118977 24.377478407564563 66-67 23.430830684423828 26.7445169272844 25.554911335584542 24.269741052707232 68-69 23.399603524956465 26.55800025835953 25.39887895273089 24.643517263953115 70-71 23.238126132706043 26.55937079332643 25.689238879813214 24.513264194154313 72-73 23.492771947815854 26.7013551019774 25.72149420532213 24.08437874488462 74-75 23.489835106398477 26.46058123124829 25.899086320251214 24.150497342102017 76-77 23.31764116154453 26.368580166079266 25.988159955235542 24.32561871714067 78-79 23.249791899171385 26.50354669860298 25.958939302548977 24.287722099676653 80-81 23.12495993192799 26.58946399118794 26.061633922552545 24.223942154331528 82-83 23.357055233792003 26.41464535159858 26.12675789577969 24.101541518829723 84-85 23.266703575279 26.68113872781841 25.669259982322718 24.382897714579872 86-87 23.13851251933915 26.6963337920666 25.86274713566883 24.302406552925422 88-89 23.37145515159225 26.21199511735156 25.951354138646625 24.46519559240956 90-91 23.468305756233978 26.252621766487998 25.989862502913073 24.28920997436495 92-93 23.326985840981237 26.59965061278711 25.876017063457766 24.19734648277389 94-95 22.62158952653661 26.823345266774055 26.104620057307702 24.45044514938163 96-97 22.952638937434465 26.787642238533536 25.773913550040778 24.485805273991225 98-99 22.721985642077218 26.982272994716265 25.705905312276084 24.58983605093043 100-101 22.955497069608093 27.22415143869282 25.476595211767776 24.343756279931306 102-103 23.07804328219248 27.30073172555001 25.633014928676907 23.988210063580613 104-105 22.940609353412746 27.49702177815621 25.67639859919202 23.88597026923902 106-107 22.845584180562785 27.29324753597974 25.841922129969785 24.019246153487686 108-109 22.747056122265704 27.36590295907163 25.75302008992067 24.134020828741996 110-111 22.69481099469527 27.934009304637563 25.459406771104366 23.911772929562805 112-113 22.620581322309715 28.04731119742998 25.28535548930081 24.046751990959496 114-115 22.567546447434967 28.054745797428126 25.38599010040354 23.99171765473337 116-117 22.60218450537001 27.862879621153063 25.43693505217591 24.098000821301014 118-119 22.571886751792437 27.625557921957906 25.61383549383495 24.188719832414705 120-121 22.58567416684706 27.643563625985657 25.81160274250524 23.959159464662044 122-123 22.57430778399638 27.82812193639941 25.75385200758657 23.843718272017643 124-125 22.496869691822376 27.753263770929387 25.581946129580196 24.167920407668042 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1812.0 1 1073.5 2 282.0 3 194.5 4 148.5 5 134.0 6 129.0 7 116.0 8 111.0 9 117.5 10 123.0 11 118.5 12 113.0 13 114.0 14 120.0 15 139.5 16 163.0 17 206.5 18 254.0 19 307.5 20 357.5 21 405.0 22 527.5 23 707.5 24 847.5 25 1034.5 26 1356.0 27 1782.0 28 2322.0 29 3191.5 30 4446.5 31 5634.0 32 6851.5 33 8076.0 34 9438.0 35 10790.5 36 11707.5 37 12524.0 38 12606.5 39 12595.0 40 12661.5 41 12540.0 42 12833.0 43 13810.0 44 15234.0 45 16529.0 46 17800.0 47 19135.0 48 20854.5 49 22375.0 50 23106.0 51 22095.0 52 20480.0 53 19944.5 54 19533.0 55 18720.0 56 17651.5 57 16041.0 58 14352.5 59 12737.0 60 10973.5 61 9188.5 62 7473.0 63 5986.5 64 4524.0 65 3467.0 66 2733.0 67 2094.5 68 1662.5 69 1405.5 70 1205.0 71 994.0 72 815.5 73 742.0 74 613.0 75 496.0 76 417.5 77 294.0 78 191.0 79 120.0 80 89.0 81 59.5 82 44.5 83 30.5 84 14.0 85 9.0 86 5.0 87 3.5 88 4.0 89 2.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 1.5 96 1.5 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.40490849842880006 2 0.35957424549466643 3 0.35240600891961116 4 0.3454315084682059 5 0.36383644021496964 6 0.29583506081376926 7 0.29389769957726775 8 0.28672946300221247 9 0.29777242205027066 10-11 0.2949632482573436 12-13 0.33119190337992044 14-15 0.3776885730559549 16-17 0.34688452939558206 18-19 0.32692970865961724 20-21 0.31830845115718587 22-23 0.3113339507057807 24-25 0.2965131372465447 26-27 0.30251895707969917 28-29 0.292832150897192 30-31 0.2917666022171162 32-33 0.28963550485696465 34-35 0.2781082054997811 36-37 0.2814017196018335 38-39 0.2749115594595537 40-41 0.28672946300221247 42-43 0.2771395248815304 44-45 0.2745240872122534 46-47 0.27394287884130303 48-49 0.2653216213388716 50-51 0.27365227465582775 52-53 0.2730710662848774 54-55 0.27549276783050414 56-57 0.27278046209940215 58-59 0.26658090614259755 60-61 0.2625124475459445 62-63 0.26444980878244595 64-65 0.26047821824761797 66-67 0.25795964864016613 68-69 0.26725898257537306 70-71 0.26328739204054513 72-73 0.2512757523742362 74-75 0.26725898257537306 76-77 0.2851795740130113 78-79 0.26958381605917475 80-81 0.2745240872122534 82-83 0.26764645482267335 84-85 0.2675495867608482 86-87 0.26009074600031773 88-89 0.24827284245765896 90-91 0.24139521006807887 92-93 0.24497932835560654 94-95 0.23761735565690106 96-97 0.22977104264907028 98-99 0.2303522510200207 100-101 0.2194061600337876 102-103 0.22289341025949014 104-105 0.22812428559804404 106-107 0.22357148669226568 108-109 0.224055827001391 110-111 0.22357148669226568 112-113 0.22289341025949014 114-115 0.21204418733508212 116-117 0.21882495166283714 118-119 0.21020369416040577 120-121 0.2110755067168314 122-123 0.20245424921440003 124-125 0.20167930471979945 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 516166.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.76138259679038 #Duplication Level Percentage of deduplicated Percentage of total 1 85.1944891203761 61.13674328905405 2 9.801302440438134 14.067100287502724 3 2.354294382229681 5.068422597259752 4 0.9216128355145794 2.6454484518189827 5 0.47274881847189276 1.696255441727105 6 0.27010621579923294 1.162991729624399 7 0.17363542370348725 0.8722222650919228 8 0.11971450916757691 0.6872702955809166 9 0.09764022309707182 0.6306117665854446 >10 0.5349913984760248 7.260397399504345 >50 0.042148008966396575 2.0450043346843656 >100 0.016009708759114026 1.8811132443724163 >500 6.53457500372001E-4 0.2874541739499666 >1k 6.53457500372001E-4 0.5589647232436544 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1230 0.23829543208967657 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1154 0.22357148669226568 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 704 0.13639023104970108 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 522 0.10113025654537493 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.9373612365014355E-4 0.0 2 0.0 0.0 0.0 1.9373612365014355E-4 0.0 3 0.0 0.0 0.0 1.9373612365014355E-4 0.0 4 0.0 0.0 0.0 1.9373612365014355E-4 0.0 5 0.0 0.0 0.0 1.9373612365014355E-4 0.0 6 0.0 0.0 0.0 1.9373612365014355E-4 0.0 7 0.0 0.0 0.0 1.9373612365014355E-4 0.0 8 0.0 0.0 0.0 1.9373612365014355E-4 0.0 9 0.0 0.0 0.0 1.9373612365014355E-4 0.0 10-11 0.0 0.0 0.0 2.9060418547521534E-4 0.0 12-13 0.0 0.0 0.0 3.874722473002871E-4 0.0 14-15 0.0 0.0 0.0 6.780764327755024E-4 1.9373612365014355E-4 16-17 0.0 0.0 0.0 7.749444946005742E-4 3.874722473002871E-4 18-19 0.0 0.0 0.0 0.0011624167419008614 3.874722473002871E-4 20-21 0.0 0.0 0.0 0.001743625112851292 3.874722473002871E-4 22-23 0.0 0.0 0.0 0.003584118287527656 3.874722473002871E-4 24-25 0.0 0.0 0.0 0.008330653316956173 3.874722473002871E-4 26-27 0.0 0.0 0.0 0.01637070244843713 3.874722473002871E-4 28-29 0.0 0.0 0.0 0.02944789079482182 3.874722473002871E-4 30-31 0.0 0.0 0.0 0.05443985074569034 3.874722473002871E-4 32-33 0.0 0.0 0.0 0.09318707547571906 3.874722473002871E-4 34-35 0.0 0.0 0.0 0.13542155043145035 3.874722473002871E-4 36-37 0.0 0.0 0.0 0.1918956304754672 3.874722473002871E-4 38-39 0.0 0.0 0.0 0.27801133743795603 3.874722473002871E-4 40-41 0.0 0.0 0.0 0.3756543437576284 3.874722473002871E-4 42-43 0.0 0.0 0.0 0.46351367583296843 3.874722473002871E-4 44-45 0.0 9.686806182507178E-5 0.0 0.5448828477660288 3.874722473002871E-4 46-47 0.0 1.9373612365014355E-4 0.0 0.645141291754978 3.874722473002871E-4 48-49 0.0 1.9373612365014355E-4 0.0 0.7544084654936589 3.874722473002871E-4 50-51 0.0 1.9373612365014355E-4 0.0 0.8626100905522642 3.874722473002871E-4 52-53 0.0 1.9373612365014355E-4 0.0 0.9616092497374875 3.874722473002871E-4 54-55 0.0 1.9373612365014355E-4 0.0 1.0622551659737371 3.874722473002871E-4 56-57 0.0 1.9373612365014355E-4 0.0 1.1723941522688437 3.874722473002871E-4 58-59 0.0 1.9373612365014355E-4 0.0 1.2938667017974836 3.874722473002871E-4 60-61 0.0 1.9373612365014355E-4 0.0 1.414370570707873 3.874722473002871E-4 62-63 0.0 1.9373612365014355E-4 0.0 1.52509076537393 3.874722473002871E-4 64-65 0.0 1.9373612365014355E-4 0.0 1.6372639809673633 3.874722473002871E-4 66-67 0.0 1.9373612365014355E-4 0.0 1.7563148289503765 3.874722473002871E-4 68-69 0.0 1.9373612365014355E-4 0.0 1.8880553930324742 3.874722473002871E-4 70-71 0.0 1.9373612365014355E-4 0.0 2.037425944366735 3.874722473002871E-4 72-73 0.0 1.9373612365014355E-4 0.0 2.1826311690425175 3.874722473002871E-4 74-75 0.0 1.9373612365014355E-4 0.0 2.3065254201167837 3.874722473002871E-4 76-77 0.0 1.9373612365014355E-4 0.0 2.4557991033892197 3.874722473002871E-4 78-79 0.0 1.9373612365014355E-4 0.0 2.6138877802877367 3.874722473002871E-4 80-81 0.0 1.9373612365014355E-4 0.0 2.77342947811363 4.8434030912535887E-4 82-83 0.0 1.9373612365014355E-4 0.0 2.9479857255224093 5.812083709504307E-4 84-85 0.0 1.9373612365014355E-4 0.0 3.109367916522979 5.812083709504307E-4 86-87 0.0 1.9373612365014355E-4 0.0 3.266875385050546 5.812083709504307E-4 88-89 0.0 1.9373612365014355E-4 0.0 3.442981521448526 5.812083709504307E-4 90-91 0.0 1.9373612365014355E-4 0.0 3.639914291138897 5.812083709504307E-4 92-93 0.0 1.9373612365014355E-4 0.0 3.8265790462758105 5.812083709504307E-4 94-95 0.0 1.9373612365014355E-4 0.0 4.010628363743447 5.812083709504307E-4 96-97 0.0 1.9373612365014355E-4 0.0 4.1961307021384595 5.812083709504307E-4 98-99 0.0 1.9373612365014355E-4 0.0 4.395872645621758 5.812083709504307E-4 100-101 0.0 1.9373612365014355E-4 0.0 4.627774785630979 5.812083709504307E-4 102-103 0.0 1.9373612365014355E-4 0.0 4.869944940193658 5.812083709504307E-4 104-105 0.0 1.9373612365014355E-4 0.0 5.107852900036035 5.812083709504307E-4 106-107 0.0 1.9373612365014355E-4 0.0 5.349248110104114 5.812083709504307E-4 108-109 0.0 1.9373612365014355E-4 0.0 5.599361445736449 5.812083709504307E-4 110-111 0.0 1.9373612365014355E-4 0.0 5.861098948787793 7.749444946005742E-4 112-113 0.0 1.9373612365014355E-4 0.0 6.123030187962787 7.749444946005742E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATTAGAC 90 0.00950832 26.453476 3 CATGGGT 820 0.0 25.405016 4 GTTAAGC 165 1.1053469E-5 25.251045 3 GGTATCA 3175 0.0 24.197174 1 TTAGACT 155 2.0992057E-4 23.040125 4 ATGGGTG 575 0.0 22.772993 5 CATGGGG 2280 0.0 22.45065 4 CCGTCGA 165 3.18772E-4 21.6606 9 ATACTAG 170 3.930814E-4 20.999004 6 ACATGGG 5455 0.0 20.949408 3 GTACATG 5745 0.0 20.836498 1 TACATGG 5745 0.0 20.407942 2 ATGGGGG 1290 0.0 20.301506 5 GTATCAA 3845 0.0 20.29054 1 ATGGGGC 570 0.0 19.840107 5 TAGCCCG 215 8.528E-5 19.37871 5 GTGCTAG 320 2.6926136E-7 18.610958 1 TATCAAC 4235 0.0 18.549984 2 AACGCAG 4210 0.0 18.230726 6 ATCAACG 4170 0.0 18.127293 3 >>END_MODULE