FastQCFastQC Report
Sun 1 Jan 2017
ERR1142522_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142522_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences516166
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25990.5035201853667232No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23820.46147944653464196No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17350.3361321745329991No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA7710.1493705513342607No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7440.14413967599570682No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT5520.10694234025487924No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGCT750.003915149331.7223287
GGTATCA30100.030.434251
GTATCAA39900.029.3698521
TATCAAC49450.023.8158042
GTACTAG1751.7517144E-523.794051
CATGGGG18250.023.4658324
CAACGCA51050.023.1858845
ATCAACG50550.023.179893
GGACTAA1300.002054777222.8766786
TCAACGC51800.022.8501824
AACGCAG53150.022.269796
ATGGGGC5450.020.7359265
CTAGACA1450.003872303820.5101264
ACGCAGA57800.020.3752847
GTGTTAT2055.9402628E-520.3119961
CGCAGAG59600.019.7599248
AGTACTT27750.019.61211612-13
CAATACC2158.573279E-519.3653764
ACATGGG46150.019.2035223
GAGTACT26800.019.19763212-13
Produced by FastQC (version 0.11.5)