##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142522_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	516166
Sequences flagged as poor quality	0
Sequence length	125
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.134377312724975	33.0	33.0	33.0	14.0	33.0
2	29.54555317475386	33.0	27.0	33.0	14.0	33.0
3	31.04768814683648	33.0	33.0	33.0	27.0	33.0
4	31.91042416586912	33.0	33.0	33.0	27.0	33.0
5	32.16698697705777	33.0	33.0	33.0	33.0	33.0
6	35.35622454791675	37.0	37.0	37.0	33.0	37.0
7	35.92785073019145	37.0	37.0	37.0	33.0	37.0
8	36.087874830965234	37.0	37.0	37.0	37.0	37.0
9	36.10652774495027	37.0	37.0	37.0	37.0	37.0
10-11	36.18245099444752	37.0	37.0	37.0	37.0	37.0
12-13	36.20443326371749	37.0	37.0	37.0	37.0	37.0
14-15	36.22728637686326	37.0	37.0	37.0	37.0	37.0
16-17	36.239172088049195	37.0	37.0	37.0	37.0	37.0
18-19	36.22471646718304	37.0	37.0	37.0	37.0	37.0
20-21	36.240219231797525	37.0	37.0	37.0	37.0	37.0
22-23	36.23227120732478	37.0	37.0	37.0	37.0	37.0
24-25	36.21157243987399	37.0	37.0	37.0	37.0	37.0
26-27	36.16902411239795	37.0	37.0	37.0	37.0	37.0
28-29	36.136397011814026	37.0	37.0	37.0	37.0	37.0
30-31	36.06064328142497	37.0	37.0	37.0	37.0	37.0
32-33	36.009761394590114	37.0	37.0	37.0	37.0	37.0
34-35	35.932316347841585	37.0	37.0	37.0	37.0	37.0
36-37	35.85653355703398	37.0	37.0	37.0	35.0	37.0
38-39	35.7914740606704	37.0	37.0	37.0	33.0	37.0
40-41	35.65216616359853	37.0	37.0	37.0	33.0	37.0
42-43	35.595175776784984	37.0	37.0	37.0	33.0	37.0
44-45	35.49717823335904	37.0	37.0	37.0	33.0	37.0
46-47	35.344390176803586	37.0	37.0	37.0	33.0	37.0
48-49	35.20828473785565	37.0	37.0	37.0	33.0	37.0
50-51	35.115217778776596	37.0	37.0	37.0	33.0	37.0
52-53	35.02794740451715	37.0	37.0	37.0	33.0	37.0
54-55	34.97474262155973	37.0	37.0	37.0	30.0	37.0
56-57	34.92620300445981	37.0	37.0	37.0	27.0	37.0
58-59	34.885397333415995	37.0	37.0	37.0	27.0	37.0
60-61	34.854727936361556	37.0	37.0	37.0	27.0	37.0
62-63	34.847899706683506	37.0	37.0	37.0	27.0	37.0
64-65	34.82248152726061	37.0	37.0	37.0	27.0	37.0
66-67	34.78041172800998	37.0	37.0	37.0	27.0	37.0
68-69	34.74435065463436	37.0	37.0	37.0	27.0	37.0
70-71	34.71323566449553	37.0	37.0	37.0	27.0	37.0
72-73	34.691248551822476	37.0	37.0	37.0	27.0	37.0
74-75	34.596802191543034	37.0	37.0	37.0	27.0	37.0
76-77	34.551649081884506	37.0	37.0	37.0	27.0	37.0
78-79	34.525314530596745	37.0	37.0	37.0	27.0	37.0
80-81	34.4986041312291	37.0	37.0	37.0	27.0	37.0
82-83	34.44653851667874	37.0	37.0	37.0	27.0	37.0
84-85	34.37761495332897	37.0	37.0	37.0	27.0	37.0
86-87	34.21919208161715	37.0	37.0	37.0	27.0	37.0
88-89	34.155564295207355	37.0	37.0	37.0	27.0	37.0
90-91	34.104975918599834	37.0	37.0	37.0	27.0	37.0
92-93	34.028041366537124	37.0	37.0	37.0	27.0	37.0
94-95	34.0391860370501	37.0	37.0	37.0	27.0	37.0
96-97	33.99624151920119	37.0	37.0	37.0	24.5	37.0
98-99	33.92194371578135	37.0	37.0	37.0	22.0	37.0
100-101	33.86464528853121	37.0	37.0	37.0	22.0	37.0
102-103	33.766475319955205	37.0	37.0	37.0	22.0	37.0
104-105	33.674529124351466	37.0	33.0	37.0	22.0	37.0
106-107	33.60020419787433	37.0	33.0	37.0	22.0	37.0
108-109	33.510811444380295	37.0	33.0	37.0	22.0	37.0
110-111	33.43790466632828	37.0	33.0	37.0	22.0	37.0
112-113	33.37913578189962	37.0	33.0	37.0	14.0	37.0
114-115	33.27027351665937	37.0	33.0	37.0	14.0	37.0
116-117	33.15393303704622	37.0	33.0	37.0	14.0	37.0
118-119	33.00500323539326	37.0	33.0	37.0	14.0	37.0
120-121	32.93008838241961	37.0	33.0	37.0	14.0	37.0
122-123	32.78078370136739	37.0	33.0	37.0	14.0	37.0
124-125	31.56300783081412	37.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	47.0
16	17.0
17	13.0
18	70.0
19	310.0
20	1277.0
21	3284.0
22	5283.0
23	7224.0
24	6169.0
25	4175.0
26	3795.0
27	4159.0
28	5323.0
29	6970.0
30	9448.0
31	12762.0
32	18165.0
33	27152.0
34	44421.0
35	91886.0
36	264214.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.433873630845795	20.207088207250994	12.627419517375566	23.731618644527646
2	16.970896959505275	23.559862524846658	35.679413212028685	23.78982730361938
3	21.15912547344357	26.776127788594735	28.876618910619666	23.18812782734203
4	15.266987752002262	18.06763715548874	34.744442679293094	31.9209324132159
5	15.4900243723821	36.66919819125152	31.882873073748737	15.957904362617647
6	29.306757285616307	35.216621395939974	18.801171743316917	16.6754495751268
7	25.30737665216161	32.46412808878177	23.551270774321793	18.677224484734822
8	25.893026661965337	32.01586311380447	21.716463308315543	20.37464691591465
9	26.631161293072385	16.084360457682216	21.46751238942511	35.816965859820286
10-11	25.02576690444547	25.563481515636443	27.20830120542616	22.202450374491928
12-13	26.289023298706233	24.450564353328193	27.285505050700742	21.974907297264835
14-15	24.3250233452029	24.595382105756673	26.3147902031517	24.764804345888724
16-17	22.945331540628402	26.74033159874924	27.131194228213406	23.183142632408956
18-19	22.852725673523633	25.319083395651788	29.799328123123182	22.0288628077014
20-21	24.04766309965137	24.17717607468162	29.752627311641927	22.022533514025085
22-23	23.836420841357235	24.353018215070346	29.50484921517496	22.305711728397455
24-25	23.085015285780155	25.01830806368494	29.48063219971869	22.416044450816212
26-27	22.844588756330328	25.022085918096117	29.373205519154688	22.760119806418867
28-29	22.60009376828385	24.822053370427344	29.915085457004142	22.66276740428467
30-31	23.118919107418932	24.51982501753312	29.7967126854539	22.564543189594044
32-33	22.689212385162914	24.747368094760212	29.645404772883143	22.918014747193734
34-35	22.87442411937245	24.809557390451907	29.71050011042959	22.60551837974605
36-37	23.056923547850886	24.788827625221344	29.30452606332071	22.849722763607055
38-39	23.004517926403523	24.77555670075131	29.30859452191737	22.911330850927804
40-41	22.54149694131095	24.837620664435413	29.59997287623328	23.020909518020353
42-43	22.933654935921652	24.751678242422482	29.22059806457237	23.09406875708349
44-45	23.04917410290488	24.989828853508367	28.841206123611396	23.119790919975355
46-47	23.063129913860912	24.97713807440588	28.570335295325705	23.3893967164075
48-49	23.167129572670106	25.01474937684488	28.58773142474621	23.230389625738802
50-51	22.91317134410248	25.408880088963627	28.158576891930114	23.51937167500378
52-53	22.77048468903415	25.448983466559206	28.022671001189543	23.757860843217106
54-55	22.81330037236083	25.405586574861577	27.92338123781884	23.857731814958754
56-57	23.117853558738855	25.180658935303757	27.640913969536935	24.060573536420453
58-59	22.95404966619266	25.106167395760277	28.123801257734915	23.81598168031215
60-61	23.03716246323857	25.346593925210108	28.199648950143946	23.416594661407377
62-63	22.703645726374848	25.315693013487913	28.170588531596426	23.810072728540817
64-65	22.52221184657649	25.143364731501105	28.390672768062984	23.943750653859418
66-67	22.993497661405637	24.948752426751813	28.3153982973018	23.74235161454075
68-69	22.858344021109488	24.97578298454373	28.301263546998445	23.864609447348332
70-71	22.679138106733106	25.0340006897006	28.615600407620807	23.67126079594549
72-73	22.92634540051069	25.0015498889892	28.666359272017143	23.40574543848297
74-75	22.864151868748987	25.16901175013303	28.650338798894676	23.31649758222331
76-77	22.741318315667485	24.990770608190275	28.807039337072894	23.460871739069347
78-79	22.680322493463823	25.281523077385064	28.662802918831265	23.37535151031985
80-81	22.543135347930704	25.45857340467989	28.70849687891105	23.289794368478358
82-83	22.70877973365158	25.215725173684433	28.80420252399422	23.271292568669768
84-85	22.81395278969541	25.3681006269316	28.19631904218729	23.621627541185696
86-87	22.427864346060957	25.52831234056509	28.36318507888177	23.68063823449218
88-89	22.774916615476286	25.096582423309044	28.36432413653227	23.7641768246824
90-91	22.936011132714956	25.102623655079785	28.224923511072102	23.736441701133153
92-93	22.795937046718535	25.32248431209811	28.17869082616486	23.7028878150185
94-95	22.267997896019583	25.28583426407667	28.290012195724422	24.15615564417933
96-97	22.56347764091397	25.255441079032714	27.934230460743247	24.24685081931007
98-99	22.41662565918716	25.6108499978689	27.647016657431912	24.325507685512022
100-101	22.668579487994172	25.696771968707743	27.41743935090649	24.217209192391596
102-103	22.76312271633544	25.655506174370263	27.480016118845484	24.101354990448808
104-105	22.654659972314597	25.81978527830222	27.45940426982901	24.066150479554167
106-107	22.5904915790473	25.683663564873203	27.403793061359714	24.322051794719783
108-109	22.561477097147616	25.86508701485525	27.23194435699098	24.341491531006156
110-111	22.544503824853944	26.260865016608108	27.0187644031397	24.17586675539824
112-113	22.487533080443114	26.346562927430323	26.86335403726708	24.302549954859483
114-115	22.364047965235955	26.35278801082211	27.001029708494663	24.282134315447273
116-117	22.418853624609135	26.234680316022363	26.969812037212836	24.376654022155662
118-119	22.388243317072416	25.983404563648126	27.11472665770314	24.513625461576314
120-121	22.31765934404442	26.11055034940513	27.394640840199703	24.177149466350745
122-123	22.092892596567772	26.394415749971905	27.33393908161328	24.178752571847042
124-125	22.19905999232805	26.273621276876042	27.23464931824258	24.292669412553323
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	158.0
1	151.0
2	264.0
3	428.0
4	357.5
5	303.0
6	480.0
7	946.0
8	1366.5
9	1498.5
10	1385.0
11	1159.5
12	1047.0
13	1026.0
14	1025.0
15	1025.5
16	1059.5
17	1044.5
18	1008.0
19	984.0
20	987.0
21	1008.0
22	1090.5
23	1171.5
24	1212.5
25	1316.5
26	1482.5
27	1773.0
28	2147.0
29	2885.0
30	4048.0
31	5047.0
32	6137.0
33	7465.5
34	8798.0
35	9805.0
36	10700.5
37	11868.0
38	12284.0
39	12203.5
40	12138.5
41	12059.0
42	12314.5
43	13120.0
44	14318.0
45	15653.0
46	17343.5
47	18846.0
48	20490.5
49	21865.5
50	22700.5
51	21898.5
52	20130.5
53	19577.5
54	19242.5
55	18586.0
56	17483.0
57	15828.0
58	14371.0
59	13004.0
60	11202.5
61	9177.5
62	7357.5
63	5893.5
64	4514.0
65	3441.5
66	2613.5
67	1983.5
68	1653.0
69	1399.5
70	1152.0
71	944.5
72	758.0
73	709.0
74	606.5
75	513.5
76	415.0
77	258.0
78	180.0
79	114.5
80	73.5
81	66.0
82	48.5
83	26.0
84	13.5
85	6.5
86	2.5
87	2.0
88	1.0
89	0.5
90	0.5
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.029835363042122108
2	0.0
3	1.9373612365014358E-4
4	0.0
5	0.0015498889892011486
6	0.0023248334838017227
7	0.0046496669676034455
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	2.9060418547521534E-4
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.002615437669276938
42-43	1.9373612365014358E-4
44-45	0.0
46-47	0.0046496669676034455
48-49	0.0082337852551311
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.008524389440606317
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.05802396903321799
76-77	0.029351022732996748
78-79	9.686806182507179E-5
80-81	0.0
82-83	0.0
84-85	0.04659353773785953
86-87	0.1684535595137998
88-89	0.18065893530375887
90-91	0.11013898629510663
92-93	0.09338081159936919
94-95	2.9060418547521534E-4
96-97	0.0
98-99	0.0
100-101	0.0
102-103	0.0
104-105	0.0018404931746763638
106-107	0.002518569607451866
108-109	0.003584118287527656
110-111	4.843403091253589E-4
112-113	0.0
114-115	9.686806182507179E-5
116-117	0.0
118-119	0.0
120-121	5.812083709504307E-4
122-123	0.0
124-125	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	516166.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.33523978371451
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.05475051587106	55.925115411627644
2	11.07494190565939	14.914677376165661
3	2.7618784809590324	5.579152493065728
4	1.0785366301150794	2.9049409041727308
5	0.4998484036581846	1.682870605791539
6	0.33944884983590257	1.371412181880396
7	0.2148077297057949	1.0124890990994522
8	0.14605720424946125	0.7867837496221151
9	0.11298824768185853	0.6847281675359701
>10	0.6393576252098507	8.038952392468893
>50	0.05423817819246423	2.5183487622801737
>100	0.021073432247423418	2.5374236333557785
>500	0.001036398307250332	0.4808264255726434
>1k	0.001036398307250332	1.562278797361332
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2599	0.5035201853667232	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2382	0.46147944653464196	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1735	0.3361321745329991	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	771	0.1493705513342607	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	744	0.14413967599570682	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	552	0.10694234025487924	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.9373612365014355E-4	0.0
2	0.0	0.0	0.0	1.9373612365014355E-4	0.0
3	0.0	0.0	0.0	1.9373612365014355E-4	0.0
4	0.0	0.0	0.0	1.9373612365014355E-4	0.0
5	0.0	0.0	0.0	1.9373612365014355E-4	0.0
6	0.0	0.0	0.0	1.9373612365014355E-4	0.0
7	0.0	0.0	0.0	1.9373612365014355E-4	0.0
8	0.0	0.0	0.0	1.9373612365014355E-4	0.0
9	0.0	0.0	0.0	1.9373612365014355E-4	0.0
10-11	0.0	0.0	0.0	2.9060418547521534E-4	0.0
12-13	0.0	0.0	0.0	3.874722473002871E-4	0.0
14-15	0.0	0.0	0.0	6.780764327755024E-4	0.0
16-17	0.0	0.0	0.0	7.749444946005742E-4	0.0
18-19	0.0	0.0	0.0	0.0011624167419008614	0.0
20-21	0.0	0.0	0.0	0.0019373612365014355	0.0
22-23	0.0	0.0	0.0	0.003777854411177799	0.0
24-25	0.0	0.0	0.0	0.008427521378781245	0.0
26-27	0.0	0.0	0.0	0.01637070244843713	0.0
28-29	0.0	0.0	0.0	0.02944789079482182	0.0
30-31	0.0	0.0	0.0	0.05434298268386527	0.0
32-33	0.0	0.0	0.0	0.0933808115993692	0.0
34-35	0.0	0.0	0.0	0.1352278143078002	1.9373612365014355E-4
36-37	0.0	0.0	0.0	0.191605026289992	1.9373612365014355E-4
38-39	0.0	0.0	0.0	0.2777207332524808	1.9373612365014355E-4
40-41	0.0	0.0	0.0	0.3760418160049287	1.9373612365014355E-4
42-43	0.0	0.0	0.0	0.4643854883893941	1.9373612365014355E-4
44-45	0.0	0.0	0.0	0.5466264728788801	3.874722473002871E-4
46-47	0.0	0.0	0.0	0.6478535974860801	3.874722473002871E-4
48-49	0.0	0.0	0.0	0.7578957157193615	3.874722473002871E-4
50-51	0.0	0.0	0.0	0.8660973407779669	3.874722473002871E-4
52-53	0.0	0.0	0.0	0.9660651805814409	3.874722473002871E-4
54-55	0.0	0.0	0.0	1.067486041312291	3.874722473002871E-4
56-57	0.0	0.0	0.0	1.1764626108654967	5.812083709504307E-4
58-59	0.0	0.0	0.0	1.2990975771360376	7.749444946005742E-4
60-61	0.0	0.0	0.0	1.4198920502319021	7.749444946005742E-4
62-63	0.0	0.0	0.0	1.5312903213307347	7.749444946005742E-4
64-65	0.0	0.0	0.0	1.6444322175424184	7.749444946005742E-4
66-67	0.0	0.0	0.0	1.7635799335872568	7.749444946005742E-4
68-69	0.0	0.0	0.0	1.89599857410213	7.749444946005742E-4
70-71	0.0	0.0	0.0	2.045756597683691	7.749444946005742E-4
72-73	0.0	0.0	0.0	2.1910586904212987	7.749444946005742E-4
74-75	0.0	0.0	0.0	2.3161153582374663	7.749444946005742E-4
76-77	0.0	0.0	0.0	2.4662608540663276	7.749444946005742E-4
78-79	0.0	0.0	0.0	2.626190024139521	7.749444946005742E-4
80-81	0.0	0.0	0.0	2.786894138707315	7.749444946005742E-4
82-83	0.0	0.0	0.0	2.962225330610695	7.749444946005742E-4
84-85	0.0	0.0	0.0	3.1242855980440405	7.749444946005742E-4
86-87	0.0	0.0	0.0	3.283536691684458	7.749444946005742E-4
88-89	0.0	0.0	0.0	3.460805244824339	7.749444946005742E-4
90-91	0.0	0.0	0.0	3.6583192228856607	7.749444946005742E-4
92-93	0.0	0.0	0.0	3.847405679568201	7.749444946005742E-4
94-95	0.0	0.0	0.0	4.033586094395989	7.749444946005742E-4
96-97	0.0	0.0	0.0	4.221025794027502	7.749444946005742E-4
98-99	0.0	0.0	0.0	4.4231894390564275	7.749444946005742E-4
100-101	0.0	0.0	0.0	4.658094488982227	7.749444946005742E-4
102-103	0.0	0.0	0.0	4.902976949276008	9.686806182507177E-4
104-105	0.0	0.0	0.0	5.143306610664011	9.686806182507177E-4
106-107	0.0	0.0	0.0	5.387607862586842	9.686806182507177E-4
108-109	0.0	0.0	0.0	5.641305316506705	9.686806182507177E-4
110-111	0.0	0.0	0.0	5.907886222649303	9.686806182507177E-4
112-113	0.0	0.0	0.0	6.172336031431748	9.686806182507177E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCGCT	75	0.0039151493	31.722328	7
GGTATCA	3010	0.0	30.43425	1
GTATCAA	3990	0.0	29.369852	1
TATCAAC	4945	0.0	23.815804	2
GTACTAG	175	1.7517144E-5	23.79405	1
CATGGGG	1825	0.0	23.465832	4
CAACGCA	5105	0.0	23.185884	5
ATCAACG	5055	0.0	23.17989	3
GGACTAA	130	0.0020547772	22.876678	6
TCAACGC	5180	0.0	22.850182	4
AACGCAG	5315	0.0	22.26979	6
ATGGGGC	545	0.0	20.735926	5
CTAGACA	145	0.0038723038	20.510126	4
ACGCAGA	5780	0.0	20.375284	7
GTGTTAT	205	5.9402628E-5	20.311996	1
CGCAGAG	5960	0.0	19.759924	8
AGTACTT	2775	0.0	19.612116	12-13
CAATACC	215	8.573279E-5	19.365376	4
ACATGGG	4615	0.0	19.203522	3
GAGTACT	2680	0.0	19.197632	12-13
>>END_MODULE