FastQCFastQC Report
Sun 1 Jan 2017
ERR1142521_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142521_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences954541
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21580.22607724550333616No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21260.22272484890643773No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG953.266086E-431.3480932
GTATCAA49750.026.8246421
GGTATCA37800.025.3754121
CATGGGG32750.024.0001094
AACGCAG57200.022.8901656
CAACGCA57150.022.6096615
ATCAACG56700.022.5790653
TCAACGC57200.022.3816974
TATCAAC60800.021.6497762
GTACATG84400.021.1766781
CATGGGT17750.020.7990684
ACGCAGA63250.020.6174137
TACATGG85550.020.6080282
ACATGGG81050.020.424073
CGCAGAG65000.019.8822468
ATGGGGG16700.018.8977435
GTATAGG2551.4794388E-518.690841
GCAGAGT68400.018.6464719
ATGGGCG2251.206196E-418.5253355
GGGTTAG1959.820907E-418.33141
Produced by FastQC (version 0.11.5)