##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142521_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	954541
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.211026032407197	33.0	33.0	33.0	27.0	33.0
2	31.286892862642883	33.0	33.0	33.0	27.0	33.0
3	31.381956353891557	33.0	33.0	33.0	27.0	33.0
4	31.358249671831803	33.0	33.0	33.0	27.0	33.0
5	31.390158201690657	33.0	33.0	33.0	27.0	33.0
6	34.770521119574745	37.0	37.0	37.0	27.0	37.0
7	34.73840306492859	37.0	37.0	37.0	27.0	37.0
8	34.757047628127026	37.0	37.0	37.0	27.0	37.0
9	34.68841568879702	37.0	37.0	37.0	27.0	37.0
10-11	34.752908465953794	37.0	37.0	37.0	27.0	37.0
12-13	34.7015350833542	37.0	37.0	37.0	27.0	37.0
14-15	34.68704487287607	37.0	37.0	37.0	27.0	37.0
16-17	34.66165727821016	37.0	37.0	37.0	27.0	37.0
18-19	34.691323892844835	37.0	37.0	37.0	27.0	37.0
20-21	34.67928355094229	37.0	37.0	37.0	27.0	37.0
22-23	34.750215024812974	37.0	37.0	37.0	27.0	37.0
24-25	34.80292622317952	37.0	37.0	37.0	27.0	37.0
26-27	34.855217324347514	37.0	37.0	37.0	27.0	37.0
28-29	34.89435236412056	37.0	37.0	37.0	27.0	37.0
30-31	34.82785076806549	37.0	37.0	37.0	27.0	37.0
32-33	34.85425874844559	37.0	37.0	37.0	27.0	37.0
34-35	34.91201111319472	37.0	37.0	37.0	27.0	37.0
36-37	34.89443041210383	37.0	37.0	37.0	27.0	37.0
38-39	34.86186083154102	37.0	37.0	37.0	27.0	37.0
40-41	34.83161278562157	37.0	37.0	37.0	27.0	37.0
42-43	34.83847157953404	37.0	37.0	37.0	27.0	37.0
44-45	34.83121364090175	37.0	37.0	37.0	27.0	37.0
46-47	34.80621261946841	37.0	37.0	37.0	27.0	37.0
48-49	34.80328451056581	37.0	37.0	37.0	27.0	37.0
50-51	34.72127336594237	37.0	37.0	37.0	27.0	37.0
52-53	34.745126191541274	37.0	37.0	37.0	27.0	37.0
54-55	34.72259022923059	37.0	37.0	37.0	27.0	37.0
56-57	34.685556199262265	37.0	37.0	37.0	27.0	37.0
58-59	34.651438754333235	37.0	37.0	37.0	27.0	37.0
60-61	34.63667406638373	37.0	37.0	37.0	27.0	37.0
62-63	34.61537482413013	37.0	37.0	37.0	27.0	37.0
64-65	34.57850108062409	37.0	37.0	37.0	27.0	37.0
66-67	34.54329044011729	37.0	37.0	37.0	27.0	37.0
68-69	34.514619068222316	37.0	37.0	37.0	27.0	37.0
70-71	34.47344378083288	37.0	37.0	37.0	27.0	37.0
72-73	34.41928057569031	37.0	37.0	37.0	27.0	37.0
74-75	34.34776295622713	37.0	37.0	37.0	27.0	37.0
76-77	34.30917425233699	37.0	37.0	37.0	27.0	37.0
78-79	34.25036797790771	37.0	37.0	37.0	27.0	37.0
80-81	34.20071426999993	37.0	37.0	37.0	27.0	37.0
82-83	34.14544320254447	37.0	37.0	37.0	27.0	37.0
84-85	34.09345800756594	37.0	37.0	37.0	27.0	37.0
86-87	34.022769058636555	37.0	37.0	37.0	27.0	37.0
88-89	33.97245849052057	37.0	37.0	37.0	27.0	37.0
90-91	33.87801676407823	37.0	37.0	37.0	27.0	37.0
92-93	33.804995280454165	37.0	35.0	37.0	22.0	37.0
94-95	33.73779806210524	37.0	33.0	37.0	22.0	37.0
96-97	33.64280319022441	37.0	33.0	37.0	22.0	37.0
98-99	33.54734998287135	37.0	33.0	37.0	22.0	37.0
100-101	33.43888633385051	37.0	33.0	37.0	22.0	37.0
102-103	33.3218028350799	37.0	33.0	37.0	18.0	37.0
104-105	33.191028462894735	37.0	33.0	37.0	18.0	37.0
106-107	33.09896274754044	37.0	33.0	37.0	14.0	37.0
108-109	32.990476050793	37.0	33.0	37.0	14.0	37.0
110-111	32.90984462689397	37.0	33.0	37.0	14.0	37.0
112-113	32.77252208129352	37.0	33.0	37.0	14.0	37.0
114-115	32.666099727513014	37.0	33.0	37.0	14.0	37.0
116-117	32.46748699112977	37.0	33.0	37.0	14.0	37.0
118-119	32.39908343381793	37.0	33.0	37.0	14.0	37.0
120-121	32.29183712381134	37.0	33.0	37.0	14.0	37.0
122-123	32.20518500514907	37.0	33.0	37.0	14.0	37.0
124-125	30.573480866720235	35.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	1908.0
1	103.0
2	71.0
3	46.0
4	56.0
5	37.0
6	29.0
7	51.0
8	61.0
9	32.0
10	44.0
11	35.0
12	37.0
13	29.0
14	77.0
15	385.0
16	477.0
17	963.0
18	1965.0
19	3620.0
20	5389.0
21	6958.0
22	7581.0
23	8739.0
24	10018.0
25	11346.0
26	13553.0
27	15886.0
28	18840.0
29	22273.0
30	26485.0
31	32021.0
32	40245.0
33	53664.0
34	80703.0
35	152444.0
36	438370.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.96436464698637	19.338564255876896	12.1435386465172	24.553532450619535
2	17.448707487571188	22.534216038426273	35.862382967268694	24.154693506733853
3	19.836972521048693	26.95620358143173	28.05416305691768	25.152660840601897
4	14.51675641125378	18.638314245525212	34.12761322851334	32.717316114707664
5	15.450667244303634	37.29207435113107	31.233195338385556	16.024063066179743
6	30.294359338647748	35.58772038594994	18.143051997318274	15.974868278084037
7	26.84814680935128	32.76887867044394	21.45334028262313	18.929634237581645
8	25.815426675954907	33.548622997222985	20.52848411490101	20.107466211921103
9	26.578998251395518	18.001378707377764	19.775518696617123	35.64410434460959
10-11	25.26405328824694	26.780044648556807	25.771958205420137	22.183943857776114
12-13	25.898212011619638	25.605093131074124	25.443685908166596	23.053008949139645
14-15	24.57605995528174	26.103232713742138	23.26064687929225	26.060060451683874
16-17	24.166327615190767	27.944622643592243	24.057410613940743	23.831639127276244
18-19	24.30379494035982	27.070115580445925	25.41476940670947	23.21132007248479
20-21	25.90310541511161	25.959754778673354	24.789614353414255	23.34752545280078
22-23	25.465260480709613	25.78669693434646	24.77530451597986	23.972738068964066
24-25	24.60827391482299	26.345303201217668	24.799047132175733	24.247375751783608
26-27	24.43717266483401	26.264680167797366	24.913306095684852	24.384841071683773
28-29	24.246883510978112	26.322353821324096	25.210200144581552	24.220562523116236
30-31	24.75034925098208	26.08204758009393	25.166981110762602	24.000622058161387
32-33	24.1446194307077	26.33868694282122	24.876815846586016	24.63987777988506
34-35	24.538667173757624	26.226197936721142	25.175279946251162	24.059854943270075
36-37	24.454888965819027	26.30333110422709	24.782320683461837	24.459459246492045
38-39	24.492092149089608	26.186597861788975	24.814615013281742	24.506694975839675
40-41	24.211760045768006	26.401816010712825	25.084777461462043	24.301646482057123
42-43	24.38738036917132	26.264229758429643	25.063548337447315	24.284841534951717
44-45	24.245450166539637	26.41905375230269	24.869901578515258	24.465594502642414
46-47	24.287250320282343	26.174381719822943	25.083346946536615	24.455021013358095
48-49	24.296354811448246	26.485064659145642	24.95095611287736	24.26762441652875
50-51	24.002985593617847	26.694484583274896	24.97430151869226	24.328228304414992
52-53	24.165328117104615	26.325216592901995	25.0501890427681	24.45926624722529
54-55	24.11689096875284	26.19299627000715	25.233997826403264	24.456114934836744
56-57	24.308843805396886	25.965780316459853	24.868649464907097	24.856726413236164
58-59	24.265336531568035	26.123141840168447	25.0384591749752	24.57306245328832
60-61	24.27021042603052	26.21433770893709	25.20217516210252	24.313276702929873
62-63	23.8306970676238	26.293067453633608	25.14917130553844	24.727064173204155
64-65	23.964377397493372	25.92312685654744	25.277825628442624	24.834670117516563
66-67	24.188343148124126	25.93126722927747	25.071648060316193	24.808741562282215
68-69	24.290436045885635	25.84452015834441	24.935253060609227	24.929790735160726
70-71	23.909045073055953	25.740801042005863	25.381456077142044	24.968697807796136
72-73	24.135834075101027	25.95903341761915	25.176304395203324	24.728828112076503
74-75	24.199120033908684	25.844920288854095	25.331668012124354	24.624291665112867
76-77	24.13492764753903	25.621113281270517	25.40047846236443	24.843480608826024
78-79	24.12284571959216	25.71002224925468	25.45564750793641	24.71148452321675
80-81	24.011160994509325	25.78574231276523	25.527413194745378	24.675683497980064
82-83	23.98477004053304	25.88389283142469	25.471786809981094	24.65955031806118
84-85	23.921125363861837	25.905334469258072	25.242320690042764	24.931219476837324
86-87	23.7421149956751	25.947211501143062	25.396449430672618	24.914224072509217
88-89	23.95407212136807	25.520745264639316	25.528254625083363	24.996927988909253
90-91	24.3065608727317	25.56025155726803	25.321023262798793	24.812164307201474
92-93	24.013606656717577	25.963761091641192	25.299286585218567	24.723345666422667
94-95	23.588661404754205	26.067206999827775	25.39606112667655	24.948070468741466
96-97	23.863111929692106	25.869358743412835	25.346743314129466	24.920786012765596
98-99	23.629507666524557	25.97598291334234	25.29202307622842	25.10248634390469
100-101	23.756819355841234	26.275571271980482	25.14567907974919	24.821930292429098
102-103	23.97583359451657	25.994425641001413	25.456729418892238	24.573011345589784
104-105	23.782937791943986	26.242079415589632	25.303080975424336	24.67190181704205
106-107	23.818301230746457	26.142875367693573	25.423999319606104	24.614824081953866
108-109	23.860725634939758	26.120295865101976	25.22434266928533	24.79463583067293
110-111	23.63680177155526	26.414674340746135	25.201770085265025	24.74675380243358
112-113	23.710239911047225	26.43557354956949	25.13719064840923	24.716995890974054
114-115	23.582200805236663	26.587192852605995	25.276609817168232	24.55399652498911
116-117	23.57116459167582	26.669476951117847	25.115281157935186	24.644077299271146
118-119	23.444463982489246	26.426908611816085	25.335268474606522	24.79335893108815
120-121	23.411047865404925	26.28670951324136	25.499450945151203	24.802791676202514
122-123	23.39274504575811	26.521806222431238	25.42314967804514	24.66229905376552
124-125	23.35450502488693	26.508581437180123	25.229042151104046	24.9078713868289
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3164.0
1	1861.0
2	473.0
3	349.5
4	294.0
5	262.0
6	221.5
7	193.5
8	203.5
9	201.5
10	189.0
11	182.5
12	188.0
13	207.5
14	204.5
15	222.0
16	253.5
17	300.0
18	352.5
19	410.0
20	488.5
21	605.5
22	778.0
23	1052.0
24	1363.0
25	1746.0
26	2293.0
27	3025.0
28	3939.5
29	5078.0
30	6494.5
31	7868.5
32	9518.5
33	11228.0
34	12689.5
35	14355.0
36	15989.5
37	17688.0
38	19126.5
39	20275.5
40	21138.5
41	21888.0
42	23010.5
43	25371.0
44	28288.5
45	30261.0
46	32681.0
47	35079.0
48	37466.5
49	40091.0
50	41477.5
51	40607.0
52	39317.0
53	39208.5
54	38547.5
55	37223.5
56	35833.5
57	33637.0
58	31351.5
59	28512.5
60	24984.5
61	21115.0
62	17040.0
63	13340.0
64	10259.0
65	8272.5
66	6562.5
67	4948.0
68	3861.5
69	3237.5
70	2803.5
71	2465.5
72	2218.0
73	2107.0
74	1692.0
75	1406.0
76	1122.5
77	745.0
78	536.5
79	332.5
80	208.5
81	168.5
82	142.0
83	86.0
84	50.5
85	33.0
86	19.5
87	11.0
88	7.0
89	6.5
90	3.0
91	1.5
92	1.5
93	2.5
94	1.5
95	0.0
96	0.5
97	1.5
98	1.0
99	0.5
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.40983048397083
2	0.3692874376270899
3	0.35755404953794545
4	0.3481254341091687
5	0.370858873531886
6	0.305382377498714
7	0.30223950568912183
8	0.2933347022286104
9	0.3064300014352448
10-11	0.3023966492796014
12-13	0.3373872887597285
14-15	0.387516094122725
16-17	0.35179211788702636
18-19	0.3319920254865951
20-21	0.32203959808955296
22-23	0.3175348151624708
24-25	0.30250141167325445
26-27	0.3055919022860202
28-29	0.2963728116445496
30-31	0.29977758943827454
32-33	0.29495851933023304
34-35	0.2835394184220479
36-37	0.2869965774125994
38-39	0.2797679722505372
40-41	0.29171088512698773
42-43	0.2835394184220479
44-45	0.2792441602822718
46-47	0.2772536748028634
48-49	0.27107269357733194
50-51	0.2762060508663326
52-53	0.27657271924411836
54-55	0.27892987310131256
56-57	0.2727488918757811
58-59	0.26792982176773966
60-61	0.2640012320057494
62-63	0.26520599953275975
64-65	0.26242979610095324
66-67	0.2610155037866367
68-69	0.2687679209169643
70-71	0.2651536183359332
72-73	0.25389166101822763
74-75	0.2695012576725358
76-77	0.28725848339673205
78-79	0.2722774611043423
80-81	0.278091773952088
82-83	0.272539367088475
84-85	0.2723822234979954
86-87	0.2615393157549021
88-89	0.2510106951927681
90-91	0.24226303532273624
92-93	0.24723924902125735
94-95	0.239801119071889
96-97	0.23210108313838798
98-99	0.23246775151617374
100-101	0.2205248386397232
102-103	0.22466295318901963
104-105	0.23011059765897954
106-107	0.22539628994459118
108-109	0.22497724036997885
110-111	0.22602486430650962
112-113	0.22267246770961122
114-115	0.21224860954113023
116-117	0.2186391155539678
118-119	0.20779620781087457
120-121	0.20900097533788492
122-123	0.2014580829948635
124-125	0.2024533257345677
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	954541.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.59429809536441
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.32296511339014	69.50410115806281
2	8.962653430296944	14.267522177129905
3	1.9447551772528282	4.643742699022944
4	0.7092416548789902	2.258063668003516
5	0.347308760189711	1.3821898494835643
6	0.19342208856018187	0.9237177225052252
7	0.1229029735863223	0.6847663141505503
8	0.0769331114219654	0.4898749603139039
9	0.06052809430709561	0.4335922063280967
>10	0.2426848873665618	3.1351522254246054
>50	0.010093440944283775	0.5751823193708924
>100	0.005697359341648928	0.889852055609302
>500	4.883450779790019E-4	0.25718641721492774
>1k	3.255633853193346E-4	0.5550562273797232
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	2158	0.22607724550333616	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2126	0.22272484890643773	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.0476239365307514E-4
7	0.0	0.0	0.0	0.0	1.0476239365307514E-4
8	0.0	0.0	0.0	0.0	1.0476239365307514E-4
9	0.0	0.0	0.0	0.0	2.0952478730615028E-4
10-11	0.0	0.0	0.0	0.0	2.0952478730615028E-4
12-13	0.0	0.0	0.0	1.0476239365307514E-4	2.0952478730615028E-4
14-15	0.0	0.0	0.0	2.619059841326879E-4	2.0952478730615028E-4
16-17	0.0	0.0	0.0	3.6666837778576297E-4	2.0952478730615028E-4
18-19	0.0	0.0	0.0	5.238119682653758E-4	2.0952478730615028E-4
20-21	0.0	0.0	0.0	8.904803460511388E-4	2.0952478730615028E-4
22-23	0.0	0.0	0.0	0.002304772660367653	2.0952478730615028E-4
24-25	0.0	0.0	0.0	0.004976213698521069	2.0952478730615028E-4
26-27	0.0	0.0	0.0	0.008957184657337924	2.0952478730615028E-4
28-29	0.0	0.0	0.0	0.01702388896862471	2.0952478730615028E-4
30-31	0.0	0.0	0.0	0.03593350102300477	2.0952478730615028E-4
32-33	0.0	0.0	0.0	0.06835746185863154	2.0952478730615028E-4
34-35	0.0	0.0	0.0	0.10659573554200395	2.0952478730615028E-4
36-37	0.0	0.0	0.0	0.16431981444484836	2.0952478730615028E-4
38-39	0.0	0.0	0.0	0.24451542678627736	2.0952478730615028E-4
40-41	0.0	0.0	0.0	0.33256821865168706	2.0952478730615028E-4
42-43	0.0	0.0	0.0	0.41747813870750444	2.0952478730615028E-4
44-45	0.0	0.0	0.0	0.5047452126205161	2.0952478730615028E-4
46-47	0.0	0.0	0.0	0.610659992603775	2.0952478730615028E-4
48-49	0.0	0.0	0.0	0.7102366477710229	5.238119682653758E-4
50-51	0.0	0.0	0.0	0.814894279030445	5.238119682653758E-4
52-53	0.0	0.0	0.0	0.9154661769373971	5.238119682653758E-4
54-55	0.0	0.0	0.0	1.0106951927680425	5.238119682653758E-4
56-57	0.0	0.0	0.0	1.1224766667958737	5.238119682653758E-4
58-59	0.0	0.0	0.0	1.244996286173145	5.238119682653758E-4
60-61	0.0	0.0	0.0	1.3685111482901207	5.238119682653758E-4
62-63	0.0	0.0	0.0	1.4795069043655538	5.238119682653758E-4
64-65	0.0	0.0	0.0	1.5912359971965584	5.238119682653758E-4
66-67	0.0	0.0	0.0	1.7202508849803204	5.238119682653758E-4
68-69	0.0	0.0	0.0	1.8580658138309407	5.238119682653758E-4
70-71	0.0	0.0	0.0	2.00572840768495	5.238119682653758E-4
72-73	0.0	0.0	0.0	2.149462411776969	6.285743619184508E-4
74-75	0.0	0.0	0.0	2.2870678158402837	6.285743619184508E-4
76-77	0.0	0.0	0.0	2.4360399396149566	6.285743619184508E-4
78-79	0.0	0.0	0.0	2.59952165491058	6.285743619184508E-4
80-81	0.0	0.0	0.0	2.7712272181079705	6.809555587449884E-4
82-83	0.0	0.0	0.0	2.9487470941531058	7.33336755571526E-4
84-85	0.0	0.0	0.0	3.1209764693187614	7.33336755571526E-4
86-87	0.0	0.0	0.0	3.2922106017447126	7.33336755571526E-4
88-89	0.0	0.0	0.0	3.4843972129012792	7.33336755571526E-4
90-91	0.0	0.0	0.0	3.687531494194592	7.33336755571526E-4
92-93	0.0	0.0	0.0	3.8900895823228128	7.33336755571526E-4
94-95	0.0	0.0	0.0	4.088876224279522	7.33336755571526E-4
96-97	0.0	0.0	0.0	4.297458150042796	7.33336755571526E-4
98-99	0.0	0.0	0.0	4.51190676985064	7.33336755571526E-4
100-101	0.0	0.0	0.0	4.751184076954264	7.33336755571526E-4
102-103	0.0	0.0	0.0	5.004551926004226	9.428615428776763E-4
104-105	0.0	0.0	0.0	5.255562621196994	9.428615428776763E-4
106-107	0.0	0.0	0.0	5.514273352323263	9.428615428776763E-4
108-109	0.0	0.0	0.0	5.777960297148054	9.428615428776763E-4
110-111	0.0	0.0	0.0	6.066737835252756	9.428615428776763E-4
112-113	0.0	0.0	0.0	6.36635828110055	9.428615428776763E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCACG	95	3.266086E-4	31.348093	2
GTATCAA	4975	0.0	26.824642	1
GGTATCA	3780	0.0	25.375412	1
CATGGGG	3275	0.0	24.000109	4
AACGCAG	5720	0.0	22.890165	6
CAACGCA	5715	0.0	22.609661	5
ATCAACG	5670	0.0	22.579065	3
TCAACGC	5720	0.0	22.381697	4
TATCAAC	6080	0.0	21.649776	2
GTACATG	8440	0.0	21.176678	1
CATGGGT	1775	0.0	20.799068	4
ACGCAGA	6325	0.0	20.617413	7
TACATGG	8555	0.0	20.608028	2
ACATGGG	8105	0.0	20.42407	3
CGCAGAG	6500	0.0	19.882246	8
ATGGGGG	1670	0.0	18.897743	5
GTATAGG	255	1.4794388E-5	18.69084	1
GCAGAGT	6840	0.0	18.646471	9
ATGGGCG	225	1.206196E-4	18.525335	5
GGGTTAG	195	9.820907E-4	18.3314	1
>>END_MODULE