FastQCFastQC Report
Sun 1 Jan 2017
ERR1142521_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142521_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences954541
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36190.37913510263047895No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29940.313658606597307No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22690.23770587119882747No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41650.036.998341
GTATCAA56300.030.6469351
ATCAACG70300.024.7077943
TATCAAC71700.024.4742452
CAACGCA72350.024.2543665
TCAACGC72300.024.1888644
AACGCAG74900.023.9832946
TACAACG1552.1095348E-423.0263982
ACGCAGA83900.021.2687997
CATGGGG31450.021.1837964
CGCAGAG86100.020.655188
CATGGGT17050.020.235324
GTACATG78050.020.0484281
GAGTACT37750.019.5373512-13
TACATGG80150.019.2963982
ACATGGG76900.019.2610263
GCAGAGT93100.019.1021589
AGTACTT39250.018.56339512-13
CAGAGTA91550.018.1911710-11
AGAGTAC88600.017.6556210-11
Produced by FastQC (version 0.11.5)