##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142521_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	954541
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.453687164825816	33.0	33.0	33.0	14.0	33.0
2	29.87204111714426	33.0	27.0	33.0	14.0	33.0
3	31.17662520520334	33.0	33.0	33.0	27.0	33.0
4	31.966020317618625	33.0	33.0	33.0	27.0	33.0
5	32.17918245523241	33.0	33.0	33.0	33.0	33.0
6	35.43814356847951	37.0	37.0	37.0	33.0	37.0
7	35.96283135035583	37.0	37.0	37.0	33.0	37.0
8	36.10691526084265	37.0	37.0	37.0	37.0	37.0
9	36.15338157292353	37.0	37.0	37.0	37.0	37.0
10-11	36.21426685705486	37.0	37.0	37.0	37.0	37.0
12-13	36.236777152579094	37.0	37.0	37.0	37.0	37.0
14-15	36.2579113940627	37.0	37.0	37.0	37.0	37.0
16-17	36.259578163745715	37.0	37.0	37.0	37.0	37.0
18-19	36.24478309470206	37.0	37.0	37.0	37.0	37.0
20-21	36.25786687004539	37.0	37.0	37.0	37.0	37.0
22-23	36.254278234250805	37.0	37.0	37.0	37.0	37.0
24-25	36.240509312852986	37.0	37.0	37.0	37.0	37.0
26-27	36.20193056139024	37.0	37.0	37.0	37.0	37.0
28-29	36.18540010329572	37.0	37.0	37.0	37.0	37.0
30-31	36.12182713995523	37.0	37.0	37.0	37.0	37.0
32-33	36.08410691630847	37.0	37.0	37.0	37.0	37.0
34-35	36.034407636759454	37.0	37.0	37.0	37.0	37.0
36-37	35.97317087479742	37.0	37.0	37.0	37.0	37.0
38-39	35.92817123622767	37.0	37.0	37.0	37.0	37.0
40-41	35.805674140765035	37.0	37.0	37.0	35.0	37.0
42-43	35.76711057984937	37.0	37.0	37.0	33.0	37.0
44-45	35.712408896003424	37.0	37.0	37.0	33.0	37.0
46-47	35.597993695399154	37.0	37.0	37.0	33.0	37.0
48-49	35.4975537981082	37.0	37.0	37.0	33.0	37.0
50-51	35.42159163409429	37.0	37.0	37.0	33.0	37.0
52-53	35.364828226341245	37.0	37.0	37.0	33.0	37.0
54-55	35.314135275488425	37.0	37.0	37.0	33.0	37.0
56-57	35.27226646105301	37.0	37.0	37.0	33.0	37.0
58-59	35.23299313492035	37.0	37.0	37.0	33.0	37.0
60-61	35.205724007664415	37.0	37.0	37.0	33.0	37.0
62-63	35.196720727553874	37.0	37.0	37.0	33.0	37.0
64-65	35.17119746558817	37.0	37.0	37.0	33.0	37.0
66-67	35.12134418532048	37.0	37.0	37.0	33.0	37.0
68-69	35.09040313616702	37.0	37.0	37.0	33.0	37.0
70-71	35.05412287162102	37.0	37.0	37.0	33.0	37.0
72-73	35.03258110442611	37.0	37.0	37.0	33.0	37.0
74-75	34.9324607324358	37.0	37.0	37.0	27.0	37.0
76-77	34.89114139675509	37.0	37.0	37.0	27.0	37.0
78-79	34.867795097329505	37.0	37.0	37.0	27.0	37.0
80-81	34.842681980134955	37.0	37.0	37.0	27.0	37.0
82-83	34.78590023896302	37.0	37.0	37.0	27.0	37.0
84-85	34.72144360483206	37.0	37.0	37.0	27.0	37.0
86-87	34.56108171361943	37.0	37.0	37.0	27.0	37.0
88-89	34.49423178260546	37.0	37.0	37.0	27.0	37.0
90-91	34.450738627256456	37.0	37.0	37.0	27.0	37.0
92-93	34.39824952516445	37.0	37.0	37.0	27.0	37.0
94-95	34.40993210349268	37.0	37.0	37.0	27.0	37.0
96-97	34.373719934502546	37.0	37.0	37.0	27.0	37.0
98-99	34.31441289583161	37.0	37.0	37.0	27.0	37.0
100-101	34.26108778983826	37.0	37.0	37.0	27.0	37.0
102-103	34.16661672992569	37.0	37.0	37.0	27.0	37.0
104-105	34.06939932386351	37.0	37.0	37.0	24.5	37.0
106-107	34.00212510515526	37.0	37.0	37.0	22.0	37.0
108-109	33.919549815042	37.0	37.0	37.0	22.0	37.0
110-111	33.84189678599452	37.0	35.0	37.0	22.0	37.0
112-113	33.78848629865034	37.0	33.0	37.0	22.0	37.0
114-115	33.683173902430596	37.0	33.0	37.0	22.0	37.0
116-117	33.552043338107005	37.0	33.0	37.0	22.0	37.0
118-119	33.408689097691976	37.0	33.0	37.0	22.0	37.0
120-121	33.32634323722082	37.0	33.0	37.0	22.0	37.0
122-123	33.19217927778901	37.0	33.0	37.0	14.0	37.0
124-125	31.90980900768013	37.0	30.0	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	5.0
15	40.0
16	22.0
17	15.0
18	74.0
19	351.0
20	1548.0
21	4008.0
22	6587.0
23	9216.0
24	8774.0
25	6626.0
26	6284.0
27	7415.0
28	9485.0
29	12316.0
30	16706.0
31	22594.0
32	32118.0
33	47919.0
34	79380.0
35	165507.0
36	517551.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.80461140884597	19.39570884534531	12.277703140293553	24.521976605515164
2	17.55199619502986	22.803630226464865	35.01578245460383	24.628591123901437
3	21.395548617913434	25.53264154797886	28.239928342072318	24.83188149203539
4	15.652758760493263	17.37620489848	34.08234952715494	32.8886868138718
5	16.30652281865554	35.878540762640306	31.40899252613339	16.40594389257076
6	30.96040296862494	34.346726508513214	18.137254901960784	16.555615620901065
7	26.836980368244706	31.32007786487636	22.491340780366986	19.351600986511944
8	26.383675504771404	31.611947522421772	21.262680178221785	20.74169679458504
9	27.002192676899163	15.667006446030083	20.78077316741764	36.55002770965312
10-11	25.722624800820498	24.90024001064386	26.79900601440902	22.57812917412662
12-13	26.669048265082377	23.62355310039066	26.808172723853662	22.899225910673298
14-15	24.818944393169073	24.127303070271473	25.277594152582232	25.776158383977222
16-17	23.975921411442776	26.176245965338314	26.01234520046808	23.83548742275083
18-19	23.800915832845316	24.974726072531194	28.19229346879809	23.032064625825395
20-21	24.996110685949283	24.1407235014617	27.874255464813626	22.98891034777539
22-23	24.670653224953146	23.958426091702716	27.947463754830853	23.423456928513286
24-25	23.87178759215162	24.55892413212214	27.943535165068866	23.625753110657374
26-27	23.737639347078858	24.671334180511888	28.011473577352884	23.57955289505637
28-29	23.603962532777533	24.59449096476736	28.260336643475764	23.541209858979343
30-31	23.999911999589333	24.359194628622554	28.207274491090484	23.433618880697633
32-33	23.543357488049228	24.500781003644683	27.987849657584118	23.96801185072197
34-35	23.918052717511724	24.537094025868992	28.02432165005047	23.520531606568813
36-37	23.8973871694152	24.604377293972643	27.722215790965173	23.776019745646984
38-39	23.919925912035207	24.420637772500083	27.66696244582475	23.992473869639962
40-41	23.628075770258675	24.80128693360097	27.85759708837035	23.713040207770003
42-43	23.938636314826482	24.579679080519014	27.78719768767839	23.69448691697611
44-45	23.750787027482318	24.70883911743969	27.450051909766053	24.090321945311935
46-47	23.77977487595548	24.735619635912563	27.393147797371597	24.09145769076036
48-49	23.88755567918522	25.00677081941845	27.17542368042361	23.930249820972723
50-51	23.72611548377702	25.223327232669945	27.02047371459162	24.030083568961416
52-53	23.9230687838448	25.011183385522468	26.90942557731936	24.156322253313373
54-55	23.88027334603752	24.945864033079772	27.056250072024145	24.11761254885856
56-57	23.96167372590596	24.783010892146066	26.764120137322543	24.491195244625427
58-59	23.811392072210623	24.82051582907387	27.004235543575394	24.363856555140114
60-61	23.868749482735684	24.879601819094205	27.18976974273499	24.061878955435127
62-63	23.45745232525371	25.031350146300685	27.106693164568103	24.404504363877507
64-65	23.577457647183305	24.595643351097543	27.380227774396282	24.44667122732287
66-67	23.832106903923613	24.759537939860518	27.100903176597978	24.30745197961789
68-69	23.91505446073034	24.642524522257293	27.027335651375893	24.415085365636468
70-71	23.420890249868783	24.629324460657006	27.553504773498467	24.39628051597574
72-73	23.70144394007172	24.780653738288873	27.428051807098907	24.089850514540494
74-75	23.683878099674967	24.760813514475416	27.487431997026974	24.06787638882264
76-77	23.667225231626304	24.61673326479647	27.505027608618875	24.21101389495835
78-79	23.624924505402348	24.74818983204973	27.476943046299336	24.149942616248584
80-81	23.479766715101814	24.7935395127082	27.642395664513103	24.084298107676883
82-83	23.586519594234296	24.89337807385958	27.43910423962931	24.08099809227681
84-85	23.467744344627743	24.87378495251442	27.209149850659287	24.44932085219855
86-87	23.320149183630523	24.948165143151318	27.27974974430236	24.451935928915795
88-89	23.45903541797557	24.593288225658046	27.289461211545756	24.658215144820623
90-91	23.765336594790828	24.660751428898095	27.17528690721314	24.398625069097935
92-93	23.58099233138887	24.919341941403946	27.089571999737828	24.41009372746936
94-95	23.07742674227718	24.91637341926643	27.276355861089257	24.729843977367132
96-97	23.349861346972002	24.81627295213092	27.089878800386785	24.743986900510297
98-99	23.231794129324985	24.858544577969933	26.93268282871034	24.976978463994737
100-101	23.39218535400784	25.053140724180523	26.69932459684812	24.855349324963516
102-103	23.59254343186935	24.85529694376669	26.860030108711936	24.692129515652024
104-105	23.422105375090293	24.991108128820297	26.751724813480234	24.835061682609176
106-107	23.34070529911987	25.047746661400478	26.84698124810768	24.764566791371973
108-109	23.479585940933568	25.05464894502523	26.61305403920266	24.852711074838542
110-111	23.321395589792104	25.244844220042108	26.581515302469107	24.85224488769669
112-113	23.310889736533056	25.2779084397632	26.49928080616757	24.911921017536176
114-115	23.204697134063075	25.306600722128316	26.60214689910685	24.886555244701764
116-117	23.2570382142584	25.448986369336403	26.46995041585519	24.824025000550005
118-119	23.077950554245444	25.17545081877049	26.662186328298105	25.084412298685965
120-121	22.979787529942605	25.25136597996196	26.759588050510487	25.009258439584947
122-123	23.011897865047178	25.422061493429826	26.610538468227134	24.95550217329586
124-125	23.043850395111367	25.326465809221393	26.5820430971535	25.047640698513735
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	320.0
1	276.5
2	480.5
3	787.5
4	636.5
5	521.5
6	807.0
7	1501.0
8	2200.5
9	2398.0
10	2079.5
11	1652.5
12	1548.5
13	1526.5
14	1454.5
15	1419.0
16	1402.0
17	1369.5
18	1319.0
19	1267.0
20	1249.5
21	1263.0
22	1358.5
23	1481.0
24	1610.0
25	1873.5
26	2353.0
27	2984.0
28	3599.0
29	4548.0
30	6019.5
31	7308.0
32	8873.0
33	10533.5
34	12043.5
35	13551.0
36	15059.0
37	16996.5
38	18229.5
39	19235.5
40	20283.5
41	21024.0
42	21937.0
43	24104.0
44	26943.0
45	28985.5
46	31460.0
47	34112.5
48	36980.5
49	39566.0
50	41280.0
51	40673.0
52	39158.0
53	38836.0
54	38201.5
55	37296.0
56	36090.0
57	33800.0
58	31396.5
59	28732.0
60	25240.5
61	20992.5
62	16850.5
63	13149.0
64	10042.5
65	8260.5
66	6529.5
67	4835.5
68	3796.5
69	3160.5
70	2697.5
71	2421.5
72	2165.0
73	2084.5
74	1667.5
75	1381.5
76	1115.0
77	764.5
78	563.0
79	321.0
80	199.5
81	158.0
82	122.5
83	85.0
84	47.0
85	25.0
86	19.5
87	8.5
88	1.0
89	0.0
90	0.0
91	0.5
92	0.5
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.031323955702269465
2	0.0
3	6.285743619184508E-4
4	0.0
5	0.001571435904796127
6	0.0026190598413268784
7	0.007019080374756035
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	2.619059841326878E-4
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	5.238119682653757E-5
36-37	1.571435904796127E-4
38-39	0.0
40-41	0.0025142974476738034
42-43	2.619059841326878E-4
44-45	0.0
46-47	0.004714307714388382
48-49	0.008119085508113324
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.008014323114460249
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.06605268919826388
76-77	0.032476342032453295
78-79	2.619059841326878E-4
80-81	0.0
82-83	0.0
84-85	0.053900251534507164
86-87	0.18391038205797341
88-89	0.20234856334091467
90-91	0.12403867408524095
92-93	0.10251000218953403
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
102-103	0.0
104-105	0.001833341888928815
106-107	0.0025142974476738034
108-109	0.004138114549296469
110-111	5.761931650919132E-4
112-113	0.0
114-115	1.571435904796127E-4
116-117	2.0952478730615028E-4
118-119	0.0
120-121	6.809555587449884E-4
122-123	0.0
124-125	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	954541.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.07028106724819
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.5380003272831	65.06899726826794
2	10.175665136295654	15.481314139284178
3	2.2176880138053643	5.061004515889243
4	0.8087492021708168	2.4608711648818704
5	0.41052607929153984	1.5614417118571426
6	0.22986029295240973	1.049132225465389
7	0.1336389385886905	0.7116166133979285
8	0.09569908709510651	0.5823885162563053
9	0.07544106799024987	0.5164940921428518
>10	0.2914186551141486	3.6221286610265824
>50	0.012171393394557526	0.6397590448621864
>100	0.009084857234676091	1.266255107761825
>500	0.0015427115877649222	0.8204383774614087
>1k	5.142371959216407E-4	1.1581585614450465
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3619	0.37913510263047895	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2994	0.313658606597307	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2269	0.23770587119882747	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	5.238119682653757E-5	0.0	0.0	0.0	0.0
12-13	1.0476239365307514E-4	0.0	0.0	1.571435904796127E-4	0.0
14-15	1.0476239365307514E-4	0.0	0.0	3.142871809592254E-4	0.0
16-17	1.0476239365307514E-4	0.0	0.0	3.6666837778576297E-4	0.0
18-19	1.0476239365307514E-4	0.0	0.0	5.238119682653758E-4	0.0
20-21	1.0476239365307514E-4	0.0	0.0	8.904803460511388E-4	0.0
22-23	1.0476239365307514E-4	0.0	0.0	0.002409535054020728	0.0
24-25	1.0476239365307514E-4	0.0	0.0	0.005080976092174144	0.0
26-27	1.0476239365307514E-4	0.0	0.0	0.009061947050991	0.0
28-29	1.0476239365307514E-4	0.0	0.0	0.017076270165451247	0.0
30-31	1.0476239365307514E-4	0.0	0.0	0.03640493179444361	0.0
32-33	1.0476239365307514E-4	0.0	0.0	0.0687241302364173	0.0
34-35	1.0476239365307514E-4	0.0	0.0	0.10685764152613664	0.0
36-37	1.0476239365307514E-4	0.0	0.0	0.1652102947908995	0.0
38-39	1.0476239365307514E-4	0.0	0.0	0.24551066952598158	2.0952478730615028E-4
40-41	1.0476239365307514E-4	0.0	0.0	0.33387774857235053	2.0952478730615028E-4
42-43	1.0476239365307514E-4	0.0	0.0	0.4193638617932598	3.66668377785763E-4
44-45	1.0476239365307514E-4	0.0	0.0	0.5075737972491491	5.238119682653758E-4
46-47	1.0476239365307514E-4	0.0	0.0	0.6146933447594184	5.238119682653758E-4
48-49	1.571435904796127E-4	0.0	0.0	0.7147414306981051	5.238119682653758E-4
50-51	2.0952478730615028E-4	0.0	0.0	0.8193990619575273	5.238119682653758E-4
52-53	2.0952478730615028E-4	0.0	0.0	0.9205995342263977	5.238119682653758E-4
54-55	2.0952478730615028E-4	0.0	0.0	1.016404743222135	5.761931650919132E-4
56-57	2.0952478730615028E-4	0.0	0.0	1.1289195540055377	6.285743619184508E-4
58-59	2.0952478730615028E-4	0.0	0.0	1.2518582229574213	6.809555587449884E-4
60-61	2.0952478730615028E-4	0.0	0.0	1.3755302286648767	7.33336755571526E-4
62-63	2.0952478730615028E-4	0.0	0.0	1.4876783710704937	7.33336755571526E-4
64-65	2.0952478730615028E-4	0.0	0.0	1.6003503254443758	7.33336755571526E-4
66-67	2.0952478730615028E-4	0.0	0.0	1.7300985499837096	7.33336755571526E-4
68-69	2.0952478730615028E-4	0.0	0.0	1.8684896719994217	7.33336755571526E-4
70-71	2.0952478730615028E-4	0.0	0.0	2.017095127396309	7.33336755571526E-4
72-73	2.0952478730615028E-4	0.0	0.0	2.160986275078807	7.33336755571526E-4
74-75	2.0952478730615028E-4	0.0	0.0	2.2997440654723054	7.33336755571526E-4
76-77	2.0952478730615028E-4	0.0	0.0	2.449344763608897	7.33336755571526E-4
78-79	2.0952478730615028E-4	0.0	0.0	2.613245528479133	8.904803460511387E-4
80-81	2.0952478730615028E-4	0.0	0.0	2.7855796660384415	9.428615428776763E-4
82-83	2.0952478730615028E-4	0.0	0.0	2.964618596791547	0.0011523863301838265
84-85	2.0952478730615028E-4	0.0	0.0	3.138576551452478	0.0011523863301838265
86-87	2.0952478730615028E-4	0.0	0.0	3.3119059317514914	0.0011523863301838265
88-89	2.0952478730615028E-4	0.0	0.0	3.5053496916318942	0.0011523863301838265
90-91	2.0952478730615028E-4	0.0	0.0	3.708169685744248	0.0011523863301838265
92-93	2.0952478730615028E-4	0.0	0.0	3.9118801602026525	0.0011523863301838265
94-95	2.0952478730615028E-4	0.0	0.0	4.112447762851465	0.0011523863301838265
96-97	2.0952478730615028E-4	0.0	0.0	4.3251678031640335	0.0011523863301838265
98-99	2.0952478730615028E-4	0.0	0.0	4.5436497751275216	0.0011523863301838265
100-101	2.0952478730615028E-4	0.0	0.0	4.785860429253432	0.001204767527010364
102-103	2.0952478730615028E-4	0.0	0.0	5.043837823624129	0.0012571487238369017
104-105	2.0952478730615028E-4	0.0	0.0	5.297729484642357	0.0012571487238369017
106-107	2.0952478730615028E-4	1.0476239365307514E-4	0.0	5.559897374759178	0.0012571487238369017
108-109	2.0952478730615028E-4	1.0476239365307514E-4	0.0	5.826098617031642	0.0013619111174899769
110-111	2.0952478730615028E-4	1.0476239365307514E-4	0.0	6.119223794472946	0.0013619111174899769
112-113	2.0952478730615028E-4	1.0476239365307514E-4	0.0	6.424501409578006	0.0013619111174899769
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4165	0.0	36.99834	1
GTATCAA	5630	0.0	30.646935	1
ATCAACG	7030	0.0	24.707794	3
TATCAAC	7170	0.0	24.474245	2
CAACGCA	7235	0.0	24.254366	5
TCAACGC	7230	0.0	24.188864	4
AACGCAG	7490	0.0	23.983294	6
TACAACG	155	2.1095348E-4	23.026398	2
ACGCAGA	8390	0.0	21.268799	7
CATGGGG	3145	0.0	21.183796	4
CGCAGAG	8610	0.0	20.65518	8
CATGGGT	1705	0.0	20.23532	4
GTACATG	7805	0.0	20.048428	1
GAGTACT	3775	0.0	19.53735	12-13
TACATGG	8015	0.0	19.296398	2
ACATGGG	7690	0.0	19.261026	3
GCAGAGT	9310	0.0	19.102158	9
AGTACTT	3925	0.0	18.563395	12-13
CAGAGTA	9155	0.0	18.19117	10-11
AGAGTAC	8860	0.0	17.65562	10-11
>>END_MODULE