FastQCFastQC Report
Sun 1 Jan 2017
ERR1142519_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142519_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences440255
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9040.20533554417326325No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4870.11061771019068493No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20050.031.8029351
ATCAACG22900.026.788723
TATTCTC2905.456968E-1226.6990365
TATCAAC24100.026.6935562
AACGCAG23100.026.2899596
CAACGCA23500.026.1047535
GGTATCA14000.025.9656721
TCAACGC23950.025.862954
ACGCAGA24800.024.2588067
CGCAGAG25250.023.8291878
GCAGAGT27600.021.5942559
CATGGGG14250.020.0621094
TAGTACT1550.00565001919.2127064
AGAGTAC25700.019.13234310-11
GTACATG33400.019.0912841
GGGCTAT2851.8383653E-618.80186
TACATGG33550.018.4646282
ACATGGG31650.018.4417693
GTACTAG2301.4165064E-418.1371061
CAGAGTA27050.017.29616210-11
Produced by FastQC (version 0.11.5)