##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142519_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	440255
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.3887883158624	33.0	33.0	33.0	27.0	33.0
2	31.459465537018318	33.0	33.0	33.0	27.0	33.0
3	31.559457587080214	33.0	33.0	33.0	27.0	33.0
4	31.540820660753425	33.0	33.0	33.0	27.0	33.0
5	31.559796027302358	33.0	33.0	33.0	27.0	33.0
6	34.97257725636279	37.0	37.0	37.0	33.0	37.0
7	34.95566887372091	37.0	37.0	37.0	33.0	37.0
8	34.959914140668474	37.0	37.0	37.0	33.0	37.0
9	34.926883283551575	37.0	37.0	37.0	33.0	37.0
10-11	35.006211173070156	37.0	37.0	37.0	33.0	37.0
12-13	34.93658788656574	37.0	37.0	37.0	33.0	37.0
14-15	34.92044156227641	37.0	37.0	37.0	33.0	37.0
16-17	34.89337997297021	37.0	37.0	37.0	30.0	37.0
18-19	34.93686272728306	37.0	37.0	37.0	30.0	37.0
20-21	34.908830109822716	37.0	37.0	37.0	27.0	37.0
22-23	34.99103246981863	37.0	37.0	37.0	30.0	37.0
24-25	35.057796050016464	37.0	37.0	37.0	33.0	37.0
26-27	35.08297350399201	37.0	37.0	37.0	33.0	37.0
28-29	35.123490931392034	37.0	37.0	37.0	33.0	37.0
30-31	35.055666602310026	37.0	37.0	37.0	33.0	37.0
32-33	35.07179589101771	37.0	37.0	37.0	33.0	37.0
34-35	35.12204631406799	37.0	37.0	37.0	33.0	37.0
36-37	35.11180225096819	37.0	37.0	37.0	33.0	37.0
38-39	35.09195352693325	37.0	37.0	37.0	33.0	37.0
40-41	35.05026632292648	37.0	37.0	37.0	33.0	37.0
42-43	35.053483776447734	37.0	37.0	37.0	33.0	37.0
44-45	35.048124382460166	37.0	37.0	37.0	33.0	37.0
46-47	35.038926304073776	37.0	37.0	37.0	33.0	37.0
48-49	35.04047427059318	37.0	37.0	37.0	33.0	37.0
50-51	34.96717470556837	37.0	37.0	37.0	27.0	37.0
52-53	34.99124030391478	37.0	37.0	37.0	27.0	37.0
54-55	34.96635926905998	37.0	37.0	37.0	27.0	37.0
56-57	34.93546808099851	37.0	37.0	37.0	27.0	37.0
58-59	34.902308889166505	37.0	37.0	37.0	27.0	37.0
60-61	34.89935264789724	37.0	37.0	37.0	27.0	37.0
62-63	34.87445003463902	37.0	37.0	37.0	27.0	37.0
64-65	34.83912505252638	37.0	37.0	37.0	27.0	37.0
66-67	34.787543582696394	37.0	37.0	37.0	27.0	37.0
68-69	34.75720889030221	37.0	37.0	37.0	27.0	37.0
70-71	34.71164325220611	37.0	37.0	37.0	27.0	37.0
72-73	34.64856617187766	37.0	37.0	37.0	27.0	37.0
74-75	34.57550737640686	37.0	37.0	37.0	27.0	37.0
76-77	34.525079783307405	37.0	37.0	37.0	27.0	37.0
78-79	34.489951278236475	37.0	37.0	37.0	27.0	37.0
80-81	34.424115569385926	37.0	37.0	37.0	27.0	37.0
82-83	34.37772427343245	37.0	37.0	37.0	27.0	37.0
84-85	34.33756345754165	37.0	37.0	37.0	27.0	37.0
86-87	34.26531101293568	37.0	37.0	37.0	27.0	37.0
88-89	34.220380234182464	37.0	37.0	37.0	27.0	37.0
90-91	34.14157476916787	37.0	37.0	37.0	27.0	37.0
92-93	34.07857491680958	37.0	37.0	37.0	27.0	37.0
94-95	34.02186914401881	37.0	37.0	37.0	27.0	37.0
96-97	33.93473668669294	37.0	37.0	37.0	22.0	37.0
98-99	33.858216261030535	37.0	37.0	37.0	22.0	37.0
100-101	33.770812370103684	37.0	35.0	37.0	22.0	37.0
102-103	33.67544604831291	37.0	33.0	37.0	22.0	37.0
104-105	33.54572350115274	37.0	33.0	37.0	22.0	37.0
106-107	33.47842273228015	37.0	33.0	37.0	22.0	37.0
108-109	33.393717277486914	37.0	33.0	37.0	22.0	37.0
110-111	33.325757799457136	37.0	33.0	37.0	22.0	37.0
112-113	33.179602730235885	37.0	33.0	37.0	14.0	37.0
114-115	33.09745488410126	37.0	33.0	37.0	14.0	37.0
116-117	32.89436235817878	37.0	33.0	37.0	14.0	37.0
118-119	32.84361676755517	37.0	33.0	37.0	14.0	37.0
120-121	32.73818468841921	37.0	33.0	37.0	14.0	37.0
122-123	32.67463288321541	37.0	33.0	37.0	14.0	37.0
124-125	31.096538369808407	35.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	802.0
1	43.0
2	33.0
3	17.0
4	12.0
5	14.0
6	19.0
7	16.0
8	17.0
9	18.0
10	11.0
11	17.0
12	8.0
13	13.0
14	38.0
15	144.0
16	230.0
17	443.0
18	782.0
19	1554.0
20	2415.0
21	3137.0
22	3361.0
23	3539.0
24	3924.0
25	4452.0
26	5165.0
27	6154.0
28	7480.0
29	8830.0
30	10741.0
31	13358.0
32	16741.0
33	22912.0
34	34863.0
35	66484.0
36	222468.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.82121554821881	20.58853036134469	12.537165058460864	24.05308903197563
2	16.87213815707002	23.231383918294597	36.59261487827837	23.303863046357012
3	19.17666175173599	27.923719060435413	28.82686605940305	24.072753128425546
4	14.113277199858718	19.341210249866123	35.58786331992662	30.957649230348537
5	14.911380979732503	37.375892344301306	32.177502438846496	15.5352242371197
6	28.809038415543885	36.84156577793469	18.76473469015865	15.584661116362767
7	25.55804079847666	33.52185242212484	22.55874233548046	18.36136444391804
8	24.665143747338117	34.40484297456664	21.17913129826975	19.750881979825493
9	25.940520988036774	18.1901633668328	20.80694832943063	35.0623673156998
10-11	24.360638860037536	27.344476029082166	26.75738898303541	21.537496127844896
12-13	25.122413592750654	26.308156425090175	26.041341502594094	22.528088479565074
14-15	23.70087015198594	26.705709902224733	24.040199790725826	25.5532201550635
16-17	23.337921259340114	28.484184188518185	24.932377464775897	23.24551708736581
18-19	23.373739928254654	27.70090234180539	26.422290496874666	22.503067233065284
20-21	24.888684869685264	26.685218239164925	25.733985030407343	22.692111860742465
22-23	24.76556368933786	26.61804621410844	25.552162577438303	23.064227519115402
24-25	23.884526690391457	27.25568683274021	25.60170818505338	23.258078291814947
26-27	23.660551587156966	27.20352418091038	25.7032645140129	23.432659717919748
28-29	23.515914137675793	27.078972840630872	25.964812389682855	23.440300632010477
30-31	23.82651870156639	26.903722092844497	25.96514203396972	23.304617171619398
32-33	23.382686863579735	27.11859195539735	25.77258658504089	23.726134595982025
34-35	23.747529216727546	26.940304965249627	25.928422433345784	23.383743384677043
36-37	23.817190771861945	27.189553498127477	25.453214260094487	23.540041469916094
38-39	23.628656432280785	26.967678909141835	25.8506104538297	23.553054204747685
40-41	23.280380504279762	27.261692342203343	26.06447484237699	23.393452311139907
42-43	23.609221341573804	26.961618733255378	25.983214671620363	23.445945253550455
44-45	23.480581523898657	27.184841850228793	25.766896311451614	23.56768031442094
46-47	23.52823973977564	27.23976140787117	25.541631229655742	23.690367622697448
48-49	23.598690764195247	27.12508894264978	25.827237797068452	23.448982496086522
50-51	23.354035145643227	27.273679476743197	25.696850326466254	23.67543505114733
52-53	23.227433411700414	27.09789433536936	25.89825182587708	23.776420427053154
54-55	23.45974477852569	27.078342608869903	25.8205661872535	23.641346425350907
56-57	23.49924801586624	26.88403049833607	25.559490996114278	24.057230489683416
58-59	23.494877049180328	26.79678961748634	25.894922586520945	23.813410746812387
60-61	23.48349360269667	27.084744489234613	25.9315336565663	23.50022825150242
62-63	23.24820243803591	27.1430718159588	25.766382972910208	23.842342773095083
64-65	23.26988752789035	26.820044624561724	25.880538226856704	24.029529620691225
66-67	23.614488279329	26.514261515764165	25.987309436642807	23.88394076826403
68-69	23.521636818395535	26.517468951612443	25.648945168815114	24.311949061176907
70-71	23.415317187010842	26.4933261234596	26.076785155249453	24.014571534280105
72-73	23.693080340627233	26.590984374448634	26.114602554135075	23.601332730789064
74-75	23.662618670570836	26.6524510546155	25.882394461603614	23.802535813210046
76-77	23.55856763690149	26.47371279637366	26.217518881491525	23.750200685233324
78-79	23.52966624619182	26.435382730779217	26.23330434030229	23.80164668272668
80-81	23.33142819408739	26.619273481600224	26.299688270642108	23.749610053670278
82-83	23.58584651115134	26.696494643487366	26.15025558705329	23.567403258308005
84-85	23.594781585038326	26.643562767048707	25.84479325463283	23.916862393280137
86-87	23.302813285839996	26.681078595098807	26.188841797779926	23.827266321281275
88-89	23.66800526332564	26.23888486702818	26.077593075721772	24.01551679392441
90-91	23.634434891084872	26.498849271660667	26.10241140757637	23.76430442967809
92-93	23.485582313363437	26.693180382430377	26.029237176721807	23.792000127484382
94-95	23.069060927542765	26.699605395395636	26.245132887397123	23.986200789664476
96-97	23.25971786744664	26.727478618130913	26.112682307819245	23.9001212066032
98-99	23.066881539802743	26.983763876274956	25.915885361539985	24.033469222382315
100-101	23.25972811132278	26.968894169164553	25.817930632654406	23.953447086858258
102-103	23.34969057053831	27.065916782935062	25.995602664803336	23.58878998172329
104-105	23.223569204435783	27.290046797895005	25.955646703206657	23.530737294462554
106-107	23.162869670805637	27.08925813767965	25.953402729649117	23.794469461865596
108-109	23.095604435623372	27.20051348349387	25.948556055281415	23.755326025601338
110-111	22.97878151619126	27.55000199154447	25.726430071183486	23.744786421080782
112-113	23.07513727275314	27.458761273435943	25.7470767304902	23.719024723320718
114-115	23.00509550371296	27.54769496161873	25.774623526707586	23.672586007960728
116-117	22.95301539904137	27.564306815543794	25.799994537732886	23.68268324768195
118-119	22.994618939194808	27.189547838415702	25.99374390520048	23.822089317189015
120-121	22.902466722347516	27.223042768809925	26.1978020477505	23.67668846109206
122-123	22.92891447140115	27.560504676874135	26.021533759851817	23.4890470918729
124-125	22.973810956912157	27.420953282533773	25.921998363824827	23.683237396729243
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1146.0
1	641.5
2	108.5
3	80.5
4	70.5
5	60.0
6	58.5
7	55.5
8	58.0
9	60.5
10	56.0
11	52.0
12	54.5
13	68.0
14	76.0
15	81.5
16	96.0
17	123.5
18	167.0
19	205.5
20	249.5
21	286.0
22	358.5
23	473.0
24	620.0
25	787.0
26	979.0
27	1294.0
28	1812.0
29	2635.0
30	3865.0
31	5309.5
32	7064.5
33	8800.0
34	10344.0
35	11520.0
36	12378.0
37	13002.5
38	12491.0
39	11584.5
40	10775.5
41	10431.5
42	10629.0
43	11198.5
44	12357.0
45	13185.5
46	14251.0
47	15416.0
48	16380.5
49	17245.0
50	17356.5
51	16902.0
52	16460.0
53	16443.0
54	16027.5
55	15153.0
56	14395.0
57	13204.0
58	12143.0
59	11012.0
60	9573.0
61	8154.5
62	6636.0
63	5233.0
64	4028.5
65	3180.0
66	2477.5
67	1904.0
68	1553.0
69	1302.5
70	1145.0
71	1066.5
72	913.5
73	844.5
74	700.0
75	561.0
76	445.5
77	306.5
78	221.5
79	145.0
80	100.5
81	70.0
82	50.0
83	31.0
84	15.0
85	8.0
86	6.5
87	3.5
88	0.5
89	1.0
90	2.5
91	2.0
92	1.5
93	1.0
94	0.0
95	1.5
96	1.5
97	1.0
98	1.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3795527591963748
2	0.342528761740355
3	0.3307174251286186
4	0.32254034593587805
5	0.3459358780706636
6	0.2816549499721752
7	0.27688498710974324
8	0.26938933118306435
9	0.28233637323823696
10-11	0.2777935514644922
12-13	0.31209185585626514
14-15	0.362517177544832
16-17	0.3229946281132525
18-19	0.3047097704739299
20-21	0.29494270366037867
22-23	0.2899455997092594
24-25	0.2708657482595314
26-27	0.27961068017399004
28-29	0.2680264846509409
30-31	0.2697300428160952
32-33	0.26757220247356645
34-35	0.25507944259576837
36-37	0.2592815527364823
38-39	0.2531487433419269
40-41	0.2620072458007291
42-43	0.25473873096273747
44-45	0.24951448592293105
46-47	0.24883306265686933
48-49	0.24247311217362663
50-51	0.24588022850393523
52-53	0.24588022850393523
54-55	0.25144518517677256
56-57	0.24610736959262244
58-59	0.23963384856503617
60-61	0.23770314931119466
62-63	0.23827100203291274
64-65	0.2350910267912914
66-67	0.23418246243654245
68-69	0.24111026564150323
70-71	0.23679458495644568
72-73	0.2286175057637051
74-75	0.24224597108493942
76-77	0.2576915651156716
78-79	0.2428138238066575
80-81	0.24803806884646398
82-83	0.24304096489534474
84-85	0.2451988052378735
86-87	0.23520459733563504
88-89	0.22464253671167847
90-91	0.21987257384924647
92-93	0.22384754290127312
94-95	0.21680616915196876
96-97	0.20953765431397714
98-99	0.21033264812438246
100-101	0.20079272239951845
102-103	0.2039726976411398
104-105	0.2087426605035718
106-107	0.20317770383073444
108-109	0.20510840308457598
110-111	0.20385912709679618
112-113	0.20272342165336
114-115	0.1926156432067779
116-117	0.19920273477870779
118-119	0.19159350830768532
120-121	0.19216136102940343
122-123	0.18296214693757026
124-125	0.18523355782444267
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	440255.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.90788471215832
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.30206723059091	65.39613187242198
2	8.730236686640506	13.079271264654441
3	1.8806348009880625	4.226231245742598
4	0.6817996785255591	2.0428868686311676
5	0.33864409659765	1.2683556473194884
6	0.1949722779022791	0.8762976549102491
7	0.126793169182528	0.6648465669589795
8	0.10099658684985913	0.6052352547256585
9	0.0798250404772818	0.5381572436294036
>10	0.5085781307599604	7.2064936140416815
>50	0.04140918617718442	2.127346480733307
>100	0.013683035428625217	1.72491197430446
>500	3.6007987970066357E-4	0.24383431192669774
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	904	0.20533554417326325	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	487	0.11061771019068493	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.2714108868723807E-4	0.0
2	0.0	0.0	0.0	4.5428217737447614E-4	0.0
3	0.0	0.0	0.0	4.5428217737447614E-4	0.0
4	0.0	0.0	0.0	4.5428217737447614E-4	0.0
5	0.0	0.0	0.0	4.5428217737447614E-4	0.0
6	0.0	0.0	0.0	4.5428217737447614E-4	0.0
7	0.0	0.0	0.0	4.5428217737447614E-4	0.0
8	0.0	0.0	0.0	4.5428217737447614E-4	0.0
9	0.0	0.0	0.0	4.5428217737447614E-4	0.0
10-11	0.0	0.0	0.0	4.5428217737447614E-4	0.0
12-13	0.0	0.0	0.0	4.5428217737447614E-4	0.0
14-15	0.0	0.0	0.0	6.814232660617142E-4	1.1357054434361903E-4
16-17	0.0	0.0	0.0	0.0012492759877798095	2.2714108868723807E-4
18-19	0.0	0.0	0.0	0.0015899876208106665	2.2714108868723807E-4
20-21	0.0	0.0	0.0	0.0018171287094979045	2.2714108868723807E-4
22-23	0.0	0.0	0.0	0.0035206868746521904	2.2714108868723807E-4
24-25	0.0	0.0	0.0	0.00829064973708419	2.2714108868723807E-4
26-27	0.0	0.0	0.0	0.014537029675983236	5.678527217180952E-4
28-29	0.0	0.0	0.0	0.02839263608590476	6.814232660617142E-4
30-31	0.0	0.0	0.0	0.048835334067756184	6.814232660617142E-4
32-33	0.0	0.0	0.0	0.0822250741047802	6.814232660617142E-4
34-35	0.0	0.0	0.0	0.11777265448433294	6.814232660617142E-4
36-37	0.0	0.0	0.0	0.16956082270502323	6.814232660617142E-4
38-39	0.0	0.0	0.0	0.24020170128675428	6.814232660617142E-4
40-41	0.0	0.0	0.0	0.3261746033548739	6.814232660617142E-4
42-43	0.0	0.0	0.0	0.4013583037103497	6.814232660617142E-4
44-45	0.0	0.0	0.0	0.4742705931789531	6.814232660617142E-4
46-47	0.0	0.0	0.0	0.5655813108312229	6.814232660617142E-4
48-49	0.0	0.0	0.0	0.6620027029789555	6.814232660617142E-4
50-51	0.0	0.0	0.0	0.7500198748452601	6.814232660617142E-4
52-53	0.0	0.0	0.0	0.8272478449989211	7.949938104053333E-4
54-55	0.0	0.0	0.0	0.9058386616847054	9.085643547489523E-4
56-57	0.0	0.0	0.0	0.9954458211718209	9.085643547489523E-4
58-59	0.0	0.0	0.0	1.098567875435827	9.085643547489523E-4
60-61	0.0	0.0	0.0	1.2010085064337712	0.0010221348990925713
62-63	0.0	0.0	0.0	1.295612769872006	0.0011357054434361904
64-65	0.0	0.0	0.0	1.3932834380075185	0.0011357054434361904
66-67	0.0	0.0	0.0	1.5014025962226436	0.0011357054434361904
68-69	0.0	0.0	0.0	1.6164495576427296	0.0011357054434361904
70-71	0.0	0.0	0.0	1.7409228742433362	0.0011357054434361904
72-73	0.0	0.0	0.0	1.8569919705625149	0.0011357054434361904
74-75	0.0	0.0	0.0	1.9622718651690496	0.0011357054434361904
76-77	0.0	0.0	0.0	2.0891301632008723	0.0011357054434361904
78-79	0.0	0.0	0.0	2.222461982260281	0.0011357054434361904
80-81	0.0	0.0	0.0	2.3689679844635494	0.0011357054434361904
82-83	0.0	0.0	0.0	2.522628930960466	0.0011357054434361904
84-85	0.0	0.0	0.0	2.665727816833426	0.0011357054434361904
86-87	0.0	0.0	0.0	2.8129152423027564	0.0011357054434361904
88-89	0.0	0.0	0.0	2.966916900432704	0.0011357054434361904
90-91	0.0	0.0	0.0	3.1411341154558157	0.0011357054434361904
92-93	0.0	0.0	0.0	3.320689146063077	0.0011357054434361904
94-95	0.0	0.0	0.0	3.4993356123155896	0.0015899876208106665
96-97	0.0	3.407116330308571E-4	0.0	3.6834334646965963	0.0015899876208106665
98-99	0.0	4.5428217737447614E-4	0.0	3.8799105064110573	0.0015899876208106665
100-101	0.0	4.5428217737447614E-4	0.0	4.1059158896548595	0.0015899876208106665
102-103	0.0	4.5428217737447614E-4	0.0	4.324539187516326	0.0015899876208106665
104-105	0.0	4.5428217737447614E-4	0.0	4.5332818480198975	0.0015899876208106665
106-107	0.0	4.5428217737447614E-4	0.0	4.743955207777311	0.0017035581651542856
108-109	0.0	4.5428217737447614E-4	0.0	4.972686284085359	0.0018171287094979045
110-111	0.0	4.5428217737447614E-4	0.0	5.2183393715006074	0.0018171287094979045
112-113	0.0	5.678527217180952E-4	0.0	5.482050175466491	0.0018171287094979045
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2005	0.0	31.802935	1
ATCAACG	2290	0.0	26.78872	3
TATTCTC	290	5.456968E-12	26.699036	5
TATCAAC	2410	0.0	26.693556	2
AACGCAG	2310	0.0	26.289959	6
CAACGCA	2350	0.0	26.104753	5
GGTATCA	1400	0.0	25.965672	1
TCAACGC	2395	0.0	25.86295	4
ACGCAGA	2480	0.0	24.258806	7
CGCAGAG	2525	0.0	23.829187	8
GCAGAGT	2760	0.0	21.594255	9
CATGGGG	1425	0.0	20.062109	4
TAGTACT	155	0.005650019	19.212706	4
AGAGTAC	2570	0.0	19.132343	10-11
GTACATG	3340	0.0	19.091284	1
GGGCTAT	285	1.8383653E-6	18.8018	6
TACATGG	3355	0.0	18.464628	2
ACATGGG	3165	0.0	18.441769	3
GTACTAG	230	1.4165064E-4	18.137106	1
CAGAGTA	2705	0.0	17.296162	10-11
>>END_MODULE