FastQCFastQC Report
Sun 1 Jan 2017
ERR1142519_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142519_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences440255
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14990.34048449194216984No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14550.3304902840399314No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7350.16694870018511998No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACGCG401.7765694E-459.46079
TAAATCG350.00781345650.966325
GTACGAA558.5456565E-443.249061
GGTATCA16350.039.2812581
GTATCAA23350.034.636291
TATCAAC29600.027.5206642
ATCAACG28850.027.4116943
CAACGCA29250.027.2401185
CGTTTCG450.00954792826.43298344-45
TCAACGC30100.026.2733354
AACGCAG30850.026.2128226
ATGGGGG8350.024.9236495
TGTTATC2251.9184336E-723.784282
ACGCAGA33950.023.2938847
CGCAGAG35000.022.5950688
GCAGAGT37200.021.25889
CATGGGG14550.021.250564
CGCACTA853.9502012E-420.98613218-19
CATAAGC2858.072857E-820.8634053
GTGTTAT1754.7946256E-420.3888441
Produced by FastQC (version 0.11.5)