##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142519_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	440255
Sequences flagged as poor quality	0
Sequence length	125
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.24607102701843	33.0	27.0	33.0	14.0	33.0
2	29.290879149583763	33.0	27.0	33.0	14.0	33.0
3	30.798528125745307	33.0	33.0	33.0	27.0	33.0
4	32.24098306663184	33.0	33.0	33.0	33.0	33.0
5	32.33222109913573	33.0	33.0	33.0	33.0	33.0
6	35.66169151968745	37.0	37.0	37.0	33.0	37.0
7	35.762776118385936	37.0	37.0	37.0	33.0	37.0
8	36.06689305061839	37.0	37.0	37.0	33.0	37.0
9	36.168257032855955	37.0	37.0	37.0	37.0	37.0
10-11	36.20253375884431	37.0	37.0	37.0	37.0	37.0
12-13	36.220733438575365	37.0	37.0	37.0	37.0	37.0
14-15	36.24655597324278	37.0	37.0	37.0	37.0	37.0
16-17	36.246359496201066	37.0	37.0	37.0	37.0	37.0
18-19	36.23252546819457	37.0	37.0	37.0	37.0	37.0
20-21	36.24363721025315	37.0	37.0	37.0	37.0	37.0
22-23	36.24322154206085	37.0	37.0	37.0	37.0	37.0
24-25	36.22854822773165	37.0	37.0	37.0	37.0	37.0
26-27	36.18609328684512	37.0	37.0	37.0	37.0	37.0
28-29	36.1597971630078	37.0	37.0	37.0	37.0	37.0
30-31	36.10239406707477	37.0	37.0	37.0	37.0	37.0
32-33	36.074824817435356	37.0	37.0	37.0	37.0	37.0
34-35	36.00860637585036	37.0	37.0	37.0	37.0	37.0
36-37	35.94727487478848	37.0	37.0	37.0	37.0	37.0
38-39	35.90033389740037	37.0	37.0	37.0	37.0	37.0
40-41	35.78876787316442	37.0	37.0	37.0	33.0	37.0
42-43	35.74869904941454	37.0	37.0	37.0	33.0	37.0
44-45	35.67370274045724	37.0	37.0	37.0	33.0	37.0
46-47	35.57369819763546	37.0	37.0	37.0	33.0	37.0
48-49	35.4620379098477	37.0	37.0	37.0	33.0	37.0
50-51	35.38071458586501	37.0	37.0	37.0	33.0	37.0
52-53	35.3160747748464	37.0	37.0	37.0	33.0	37.0
54-55	35.261505263994735	37.0	37.0	37.0	33.0	37.0
56-57	35.22071526728827	37.0	37.0	37.0	33.0	37.0
58-59	35.17310763080487	37.0	37.0	37.0	33.0	37.0
60-61	35.14702161247459	37.0	37.0	37.0	33.0	37.0
62-63	35.13510465525661	37.0	37.0	37.0	33.0	37.0
64-65	35.10559562071981	37.0	37.0	37.0	33.0	37.0
66-67	35.066124178033185	37.0	37.0	37.0	33.0	37.0
68-69	35.03676051379314	37.0	37.0	37.0	33.0	37.0
70-71	35.00475519869167	37.0	37.0	37.0	33.0	37.0
72-73	34.96633996206744	37.0	37.0	37.0	27.0	37.0
74-75	34.876383005303744	37.0	37.0	37.0	27.0	37.0
76-77	34.8230559562072	37.0	37.0	37.0	27.0	37.0
78-79	34.80735596415714	37.0	37.0	37.0	27.0	37.0
80-81	34.77516893618471	37.0	37.0	37.0	27.0	37.0
82-83	34.75035490795108	37.0	37.0	37.0	27.0	37.0
84-85	34.66847849541743	37.0	37.0	37.0	27.0	37.0
86-87	34.492323766907816	37.0	37.0	37.0	27.0	37.0
88-89	34.416643763273555	37.0	37.0	37.0	27.0	37.0
90-91	34.384399950028964	37.0	37.0	37.0	27.0	37.0
92-93	34.33485707146994	37.0	37.0	37.0	27.0	37.0
94-95	34.36094876832745	37.0	37.0	37.0	27.0	37.0
96-97	34.3208492805306	37.0	37.0	37.0	27.0	37.0
98-99	34.246880784999604	37.0	37.0	37.0	27.0	37.0
100-101	34.19926065575632	37.0	37.0	37.0	27.0	37.0
102-103	34.10996808667704	37.0	37.0	37.0	27.0	37.0
104-105	34.0381006462164	37.0	37.0	37.0	24.5	37.0
106-107	33.968124155319074	37.0	37.0	37.0	22.0	37.0
108-109	33.859157760843146	37.0	35.0	37.0	22.0	37.0
110-111	33.795703626307485	37.0	33.0	37.0	22.0	37.0
112-113	33.75915889654859	37.0	33.0	37.0	22.0	37.0
114-115	33.64772688555496	37.0	33.0	37.0	22.0	37.0
116-117	33.5116693734313	37.0	33.0	37.0	22.0	37.0
118-119	33.37247844998922	37.0	33.0	37.0	22.0	37.0
120-121	33.30290967734608	37.0	33.0	37.0	22.0	37.0
122-123	33.182476065007776	37.0	33.0	37.0	14.0	37.0
124-125	31.922929892902978	37.0	30.0	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	29.0
16	17.0
17	8.0
18	43.0
19	198.0
20	847.0
21	2040.0
22	3355.0
23	4629.0
24	3846.0
25	2791.0
26	2791.0
27	3315.0
28	4345.0
29	5742.0
30	7745.0
31	10753.0
32	15042.0
33	22883.0
34	38036.0
35	79349.0
36	232450.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.31419665009588	21.581025692292314	12.892268683031455	24.212508974580352
2	17.053298656460463	21.54433226198453	35.973924203018704	25.428444878536304
3	19.453088444398006	25.820549046686686	30.361336864628147	24.36502564428716
4	15.17824896934731	18.030005337815584	35.52214057761979	31.26960511521732
5	15.895121319637836	34.61959558606403	32.970502587213396	16.51478050708474
6	29.18539651244404	35.93972529307145	18.819264465043805	16.055613729440704
7	25.370608465728655	32.22351449950253	23.736205823425454	18.669671211343367
8	24.935775857173684	32.865044122156476	21.839842818366627	20.35933720230321
9	25.87205142474248	16.38891097205029	21.83552713768157	35.90351046552566
10-11	24.66695437871234	25.58119726067847	27.68259304266845	22.06925531794074
12-13	25.83377815129868	24.455713166233206	27.454770530715155	22.25573815175296
14-15	23.910006700662116	25.093752484355658	25.77517575041737	25.221065064564858
16-17	23.055047642843352	27.05375293863784	26.717129845203345	23.174069573315464
18-19	22.9838389115399	25.793574178601038	28.997285663990187	22.225301245868874
20-21	24.166731023454645	24.87722996270335	28.630404266627902	22.325634747214107
22-23	23.854357133933743	25.001192490715606	28.516995831961022	22.62745454338963
24-25	23.166914629021818	25.708396270343325	28.512680151275966	22.612008949358895
26-27	23.07526319973652	25.697607068630678	28.42023372818026	22.806896003452547
28-29	22.673450613848793	25.52588840558313	28.863272421664714	22.93738855890336
30-31	23.145109084507844	25.241280621458017	28.904612099805792	22.708998194228343
32-33	22.74556790950699	25.39039874618119	28.65907258293489	23.20496076137693
34-35	23.13718542343122	25.25312063817633	28.795276368555005	22.81441756983745
36-37	23.300159339654677	25.389405446168066	28.318506681930565	22.99192853224669
38-39	23.091276646488968	25.307037966632972	28.52017580720264	23.081509579675416
40-41	22.635072209628255	25.534701053501905	28.82490698382268	23.00531975304716
42-43	23.137124406733847	25.35153042508464	28.443044745811218	23.068300422370296
44-45	22.969415452408263	25.642866066257053	28.052151593962588	23.335566887372092
46-47	23.154652453062056	25.55972882695739	27.882917211356194	23.40270150862436
48-49	23.295027338168715	25.61053729098851	27.777828256785703	23.316607114057067
50-51	22.980999647931313	25.82310252013038	27.478961056660346	23.716936775277965
52-53	22.968393317509168	25.588920057693837	27.753688203427558	23.688998421369433
54-55	23.165665353034036	25.717595484435158	27.44488989335726	23.67184926917355
56-57	23.304221417133252	25.51214636971755	27.197419677232514	23.986212535916685
58-59	23.105359393987577	25.57915299088028	27.600140827474984	23.715346787657154
60-61	23.18193226872184	25.73590957048707	27.725327055146508	23.356831105644577
62-63	22.9216022532396	25.888519153672302	27.416724398360042	23.773154194728054
64-65	22.908655211184428	25.605387786623663	27.719049187402756	23.766907814789157
66-67	23.170458843345415	25.43497495039485	27.705283530182754	23.689282676076974
68-69	23.118760718220123	25.2940909245778	27.62069709600118	23.966451261200895
70-71	22.900137420358654	25.516234909313923	27.971516507478622	23.612111162848805
72-73	23.207459313352487	25.403459358780704	28.040567398439542	23.348513929427263
74-75	23.095449720054468	25.521871668329897	27.83588349355659	23.546795118059045
76-77	22.97181682355548	25.52290273651201	28.054218955046668	23.45106148488584
78-79	23.047263625089155	25.663026343883306	27.86028065616667	23.429429374860874
80-81	22.91785442527626	25.696812074820276	28.093264131014976	23.292069368888484
82-83	23.05345765522254	25.69465423447775	27.897354942022233	23.354533168277474
84-85	23.03279554801487	25.738351551819555	27.685129101432217	23.543723798733364
86-87	22.91418606186581	25.831377307673918	27.785088368344486	23.469348262115787
88-89	23.12734850037576	25.36334859146911	27.767643642822982	23.74165926533215
90-91	23.186401193899464	25.581934990718157	27.74280657372693	23.488857241655445
92-93	23.075296180440663	25.842830778887187	27.552882663764844	23.52899037690731
94-95	22.612401023045788	25.611124487227826	27.86096208098052	23.915512408745858
96-97	22.82336373238237	25.739855311126508	27.453861966360403	23.98291899013072
98-99	22.651872210423505	25.85399370819184	27.430012152048246	24.06412192933641
100-101	22.923218274884185	25.826538967801575	27.21528116123742	24.034961596076815
102-103	23.08503026655007	25.752007359371277	27.307355964157136	23.855606409921524
104-105	23.043454064938622	26.177039454033142	27.067904003779702	23.71160247724854
106-107	22.83375317722258	25.969975672526306	27.238366084185323	23.957905066065788
108-109	22.918486229623262	25.930717099935602	27.12234391131171	24.028452759129426
110-111	22.861591612975765	26.175152157346425	27.119953026849426	23.843303202828384
112-113	22.93171003168618	26.114865248549137	26.90349910847123	24.049925611293453
114-115	22.822000885850247	26.21196806396293	26.96880217146881	23.997228878718015
116-117	22.835288639538447	26.175852630861662	27.014798241927974	23.974060487671917
118-119	22.713881727635123	25.946553701831892	27.13075376770281	24.20881080283018
120-121	22.743513388892296	25.977449330271458	27.284311520875498	23.99472575996075
122-123	22.691167618766396	26.390955241848474	26.902136261939102	24.015740877446028
124-125	22.608601833028587	26.186187550396927	27.161985667397303	24.04322494917718
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	88.0
1	81.5
2	119.5
3	166.5
4	154.5
5	160.0
6	230.5
7	406.5
8	630.0
9	788.0
10	765.0
11	638.5
12	579.0
13	590.5
14	618.0
15	624.5
16	611.0
17	592.5
18	603.0
19	611.5
20	608.0
21	624.5
22	686.0
23	758.0
24	808.5
25	898.0
26	1082.0
27	1333.0
28	1681.5
29	2422.5
30	3714.0
31	4958.0
32	6737.5
33	8737.5
34	10211.0
35	11015.5
36	11724.0
37	12467.0
38	11899.0
39	11005.5
40	10403.0
41	10035.0
42	10192.0
43	10855.0
44	11843.5
45	12890.0
46	14133.5
47	15190.0
48	16170.0
49	17000.5
50	17162.5
51	16547.0
52	16138.5
53	16181.0
54	15916.5
55	15361.5
56	14531.5
57	13220.0
58	12235.0
59	11343.0
60	9854.0
61	8223.5
62	6623.5
63	5225.5
64	3964.0
65	3086.0
66	2458.5
67	1900.5
68	1532.0
69	1302.5
70	1117.0
71	978.5
72	896.0
73	840.5
74	640.5
75	515.0
76	420.0
77	288.0
78	219.5
79	162.0
80	100.5
81	64.0
82	48.0
83	29.5
84	19.0
85	8.5
86	2.5
87	0.5
88	0.0
89	0.0
90	0.0
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.027938353908530285
2	0.0
3	2.2714108868723807E-4
4	0.0
5	0.002952834152934095
6	9.085643547489523E-4
7	0.007495655926678856
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	2.2714108868723807E-4
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	1.1357054434361903E-4
36-37	1.1357054434361903E-4
38-39	0.0
40-41	0.0020442697981851426
42-43	3.407116330308571E-4
44-45	0.0
46-47	0.0044292512294011425
48-49	0.006359950483242666
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	1.1357054434361903E-4
62-63	0.0
64-65	0.0
66-67	0.006473521027586285
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.07904509886315886
76-77	0.04258895412885714
78-79	2.2714108868723807E-4
80-81	0.0
82-83	0.0
84-85	0.061668805578585145
86-87	0.23032106392885943
88-89	0.26007654654688755
90-91	0.15604592792813257
92-93	0.12072548863726704
94-95	2.2714108868723807E-4
96-97	0.0
98-99	0.0
100-101	1.1357054434361903E-4
102-103	0.0
104-105	0.0019306992538415237
106-107	0.002725693064246857
108-109	0.003747827963339428
110-111	7.949938104053333E-4
112-113	0.0
114-115	0.0
116-117	0.0
118-119	0.0
120-121	4.5428217737447614E-4
122-123	0.0
124-125	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	440255.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.80828398309997
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.98929590076474	61.74743779548929
2	9.572604040394014	13.747845387807672
3	2.071241205221035	4.4619682998603105
4	0.7519085185009057	2.1597304170330003
5	0.37439169869149813	1.3442212710277146
6	0.22200258991431815	0.9564975012930623
7	0.15698749410412433	0.7891101790896934
8	0.11755149842100455	0.6752937105003539
9	0.0990594270905461	0.640195872454901
>10	0.5776913939480535	7.954551015247513
>50	0.048954869537226706	2.4129895911880928
>100	0.01719025953020137	2.1202213921592223
>500	3.7370129413481236E-4	0.19723613760752162
>1k	7.474025882696247E-4	0.7927014292416583
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1499	0.34048449194216984	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1455	0.3304902840399314	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	735	0.16694870018511998	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.2714108868723807E-4	0.0
2	0.0	0.0	0.0	4.5428217737447614E-4	0.0
3	0.0	0.0	0.0	4.5428217737447614E-4	0.0
4	0.0	0.0	0.0	4.5428217737447614E-4	0.0
5	0.0	0.0	0.0	4.5428217737447614E-4	0.0
6	0.0	0.0	0.0	4.5428217737447614E-4	0.0
7	0.0	0.0	0.0	4.5428217737447614E-4	0.0
8	0.0	0.0	0.0	4.5428217737447614E-4	0.0
9	0.0	0.0	0.0	4.5428217737447614E-4	0.0
10-11	0.0	0.0	0.0	4.5428217737447614E-4	0.0
12-13	0.0	0.0	0.0	4.5428217737447614E-4	0.0
14-15	0.0	0.0	0.0	6.814232660617142E-4	0.0
16-17	0.0	0.0	0.0	0.0012492759877798095	0.0
18-19	0.0	0.0	0.0	0.0013628465321234285	0.0
20-21	0.0	0.0	0.0	0.0015899876208106665	2.2714108868723807E-4
22-23	0.0	0.0	0.0	0.003293545785964952	2.2714108868723807E-4
24-25	0.0	0.0	0.0	0.007836367559709713	2.2714108868723807E-4
26-27	0.0	0.0	0.0	0.013969176954265142	2.2714108868723807E-4
28-29	0.0	0.0	0.0	0.027824783364186666	2.2714108868723807E-4
30-31	0.0	0.0	0.0	0.04826748134603809	2.2714108868723807E-4
32-33	0.0	0.0	0.0	0.08131650975003124	2.2714108868723807E-4
34-35	0.0	0.0	0.0	0.11743194285130208	4.5428217737447614E-4
36-37	0.0	0.0	0.0	0.16797083508421257	4.5428217737447614E-4
38-39	0.0	0.0	0.0	0.23770314931119463	4.5428217737447614E-4
40-41	0.0	0.0	0.0	0.3229946281132525	4.5428217737447614E-4
42-43	0.0	0.0	0.0	0.39874618119044647	4.5428217737447614E-4
44-45	0.0	0.0	0.0	0.4724534644694552	4.5428217737447614E-4
46-47	0.0	0.0	0.0	0.5645591759321302	4.5428217737447614E-4
48-49	0.0	0.0	0.0	0.6626841262450172	6.814232660617142E-4
50-51	0.0	0.0	0.0	0.7504741570226345	6.814232660617142E-4
52-53	0.0	0.0	0.0	0.8286106915310445	7.949938104053333E-4
54-55	0.0	0.0	0.0	0.9074286493055161	9.085643547489523E-4
56-57	0.0	0.0	0.0	0.9970358087926315	9.085643547489523E-4
58-59	0.0	0.0	0.0	1.100044292512294	9.085643547489523E-4
60-61	0.0	0.0	0.0	1.202939205687613	9.085643547489523E-4
62-63	0.0	0.0	0.0	1.2986791745692838	9.085643547489523E-4
64-65	0.0	0.0	0.0	1.3965769837934832	9.085643547489523E-4
66-67	0.0	0.0	0.0	1.5044690009199213	9.085643547489523E-4
68-69	0.0	0.0	0.0	1.6197431034286947	9.085643547489523E-4
70-71	0.0	0.0	0.0	1.743989278940614	9.085643547489523E-4
72-73	0.0	0.0	0.0	1.8593769519937309	9.085643547489523E-4
74-75	0.0	0.0	0.0	1.964316134967235	0.0011357054434361904
76-77	0.0	0.0	0.0	2.090038727555621	0.0011357054434361904
78-79	0.0	0.0	0.0	2.223711258248061	0.0011357054434361904
80-81	0.0	0.0	0.0	2.3688544139192063	0.0012492759877798095
82-83	0.0	0.0	0.0	2.5227425015048097	0.0013628465321234285
84-85	0.0	0.0	0.0	2.666295669555144	0.0013628465321234285
86-87	0.0	0.0	0.0	2.813369524480131	0.0013628465321234285
88-89	0.0	0.0	0.0	2.9670304709770474	0.0014764170764670474
90-91	0.0	0.0	0.0	3.1409069743671285	0.0015899876208106665
92-93	0.0	0.0	0.0	3.320689146063077	0.0015899876208106665
94-95	0.0	0.0	0.0	3.5014934526581185	0.0015899876208106665
96-97	0.0	0.0	0.0	3.685591305039125	0.0015899876208106665
98-99	0.0	0.0	0.0	3.881727635120555	0.0015899876208106665
100-101	0.0	0.0	0.0	4.108300871086075	0.0015899876208106665
102-103	0.0	2.2714108868723807E-4	0.0	4.329309150378758	0.0018171287094979045
104-105	0.0	2.2714108868723807E-4	0.0	4.541686068301326	0.0020442697981851426
106-107	0.0	2.2714108868723807E-4	0.0	4.757129390921171	0.0020442697981851426
108-109	0.0	2.2714108868723807E-4	0.0	4.9874504548500305	0.0020442697981851426
110-111	0.0	2.2714108868723807E-4	0.0	5.234352818253058	0.0020442697981851426
112-113	0.0	2.2714108868723807E-4	0.0	5.499085757118033	0.0020442697981851426
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCACGCG	40	1.7765694E-4	59.4607	9
TAAATCG	35	0.007813456	50.96632	5
GTACGAA	55	8.5456565E-4	43.24906	1
GGTATCA	1635	0.0	39.281258	1
GTATCAA	2335	0.0	34.63629	1
TATCAAC	2960	0.0	27.520664	2
ATCAACG	2885	0.0	27.411694	3
CAACGCA	2925	0.0	27.240118	5
CGTTTCG	45	0.009547928	26.432983	44-45
TCAACGC	3010	0.0	26.273335	4
AACGCAG	3085	0.0	26.212822	6
ATGGGGG	835	0.0	24.923649	5
TGTTATC	225	1.9184336E-7	23.78428	2
ACGCAGA	3395	0.0	23.293884	7
CGCAGAG	3500	0.0	22.595068	8
GCAGAGT	3720	0.0	21.2588	9
CATGGGG	1455	0.0	21.25056	4
CGCACTA	85	3.9502012E-4	20.986132	18-19
CATAAGC	285	8.072857E-8	20.863405	3
GTGTTAT	175	4.7946256E-4	20.388844	1
>>END_MODULE