FastQCFastQC Report
Sun 1 Jan 2017
ERR1142509_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142509_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences643155
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14860.2310485030824607No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11380.17694023991106342No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAATAC2451.6028935E-824.3071273
GGTATCA27450.023.8755531
GTATACA2052.2341555E-623.250871
TAAGACC2403.5277844E-722.3321764
GTATCAA34150.022.157261
CATGGGG22250.021.6797734
ATGGGGG13300.020.1477585
GTGAGCG1805.7304994E-419.8585689
TACATGG54200.019.669232
GTACATG54200.019.566981
GTACTAG2158.4663625E-519.3982561
ACATGGG52050.019.2215443
ATCAACG39550.019.1230433
TGGGGGG8150.018.9850036
AACGCAG38450.018.8824546
GTACAAA5700.018.8148481
CAACGCA38950.018.8045755
TCGCTAA958.3341805E-418.78552486-87
TATCAAC41100.018.6931082
TCAACGC40050.018.289524
Produced by FastQC (version 0.11.5)