FastQCFastQC Report
Sun 1 Jan 2017
ERR1142509_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142509_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences643155
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21440.3333566558605624No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20360.316564436255646No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11540.17942797614882883No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA6580.1023081527781017No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27500.030.290791
GTATCAA36700.027.885451
CGAATTA557.7695487E-427.03483466-67
TATCAAC44400.023.1744542
CAACGCA45550.022.9810945
ATCAACG44700.022.3536343
TCAACGC46400.022.303744
AACGCAG47450.022.0591666
ACGCAGA52500.019.7107227
CGCAGAG54100.019.4560598
CATGGGG19800.018.323614
GAGTACT22850.018.2175812-13
GCAGAGT58700.017.8300869
CAGAGTA57800.016.97598510-11
GTGTTAA2853.8880236E-516.7016891
ATTATAC3307.523E-616.22093
GTACTTT26650.016.0668614-15
GCTTAGA2603.6597202E-416.0191671
ACATGGG46150.015.594093
AGTACTT24450.015.56610512-13
Produced by FastQC (version 0.11.5)