##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142509_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	643155
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.24146123407266	33.0	27.0	33.0	14.0	33.0
2	29.342881575980908	33.0	27.0	33.0	14.0	33.0
3	30.810983355489736	33.0	33.0	33.0	27.0	33.0
4	32.21938412979764	33.0	33.0	33.0	33.0	33.0
5	32.319336707325604	33.0	33.0	33.0	33.0	33.0
6	35.65141062418857	37.0	37.0	37.0	33.0	37.0
7	35.73868507591482	37.0	37.0	37.0	33.0	37.0
8	36.050367329803855	37.0	37.0	37.0	33.0	37.0
9	36.152123516104204	37.0	37.0	37.0	37.0	37.0
10-11	36.19171272865795	37.0	37.0	37.0	37.0	37.0
12-13	36.2135465012322	37.0	37.0	37.0	37.0	37.0
14-15	36.24302539823215	37.0	37.0	37.0	37.0	37.0
16-17	36.23437040837745	37.0	37.0	37.0	37.0	37.0
18-19	36.231127799675036	37.0	37.0	37.0	37.0	37.0
20-21	36.24014973062481	37.0	37.0	37.0	37.0	37.0
22-23	36.227869642621144	37.0	37.0	37.0	37.0	37.0
24-25	36.22207865911017	37.0	37.0	37.0	37.0	37.0
26-27	36.18314869665944	37.0	37.0	37.0	37.0	37.0
28-29	36.164309536581385	37.0	37.0	37.0	37.0	37.0
30-31	36.09513336598487	37.0	37.0	37.0	37.0	37.0
32-33	36.06318694560409	37.0	37.0	37.0	37.0	37.0
34-35	36.00652797537141	37.0	37.0	37.0	37.0	37.0
36-37	35.95319868460946	37.0	37.0	37.0	37.0	37.0
38-39	35.910234702365685	37.0	37.0	37.0	37.0	37.0
40-41	35.78054901229097	37.0	37.0	37.0	33.0	37.0
42-43	35.74660696099696	37.0	37.0	37.0	33.0	37.0
44-45	35.68518397586895	37.0	37.0	37.0	33.0	37.0
46-47	35.58074569893727	37.0	37.0	37.0	33.0	37.0
48-49	35.47939843428101	37.0	37.0	37.0	33.0	37.0
50-51	35.40547068747036	37.0	37.0	37.0	33.0	37.0
52-53	35.34740770109849	37.0	37.0	37.0	33.0	37.0
54-55	35.30251105876499	37.0	37.0	37.0	33.0	37.0
56-57	35.25708732731612	37.0	37.0	37.0	33.0	37.0
58-59	35.21832295480872	37.0	37.0	37.0	33.0	37.0
60-61	35.179241395931	37.0	37.0	37.0	33.0	37.0
62-63	35.16606416804658	37.0	37.0	37.0	33.0	37.0
64-65	35.13669022241917	37.0	37.0	37.0	33.0	37.0
66-67	35.09674028811095	37.0	37.0	37.0	33.0	37.0
68-69	35.05898267136227	37.0	37.0	37.0	33.0	37.0
70-71	35.037270953347175	37.0	37.0	37.0	33.0	37.0
72-73	35.0100014770934	37.0	37.0	37.0	33.0	37.0
74-75	34.90846840963687	37.0	37.0	37.0	27.0	37.0
76-77	34.86035870046878	37.0	37.0	37.0	27.0	37.0
78-79	34.84631620682417	37.0	37.0	37.0	27.0	37.0
80-81	34.81819779057925	37.0	37.0	37.0	27.0	37.0
82-83	34.77556498822213	37.0	37.0	37.0	27.0	37.0
84-85	34.69777580831992	37.0	37.0	37.0	27.0	37.0
86-87	34.53634271676346	37.0	37.0	37.0	27.0	37.0
88-89	34.45744260714758	37.0	37.0	37.0	27.0	37.0
90-91	34.41830507420451	37.0	37.0	37.0	27.0	37.0
92-93	34.37577566838476	37.0	37.0	37.0	27.0	37.0
94-95	34.384229307087715	37.0	37.0	37.0	27.0	37.0
96-97	34.34692336994971	37.0	37.0	37.0	27.0	37.0
98-99	34.280719266739744	37.0	37.0	37.0	27.0	37.0
100-101	34.23941507101709	37.0	37.0	37.0	27.0	37.0
102-103	34.135722337539164	37.0	37.0	37.0	27.0	37.0
104-105	34.05039065233109	37.0	37.0	37.0	24.5	37.0
106-107	33.9867528045339	37.0	37.0	37.0	22.0	37.0
108-109	33.88363225039065	37.0	37.0	37.0	22.0	37.0
110-111	33.81690960965864	37.0	33.0	37.0	22.0	37.0
112-113	33.763018245990466	37.0	33.0	37.0	22.0	37.0
114-115	33.65007113370805	37.0	33.0	37.0	22.0	37.0
116-117	33.528333760913	37.0	33.0	37.0	22.0	37.0
118-119	33.388512877922125	37.0	33.0	37.0	22.0	37.0
120-121	33.31344310469483	37.0	33.0	37.0	22.0	37.0
122-123	33.177200674798456	37.0	33.0	37.0	14.0	37.0
124-125	31.905243681538664	37.0	30.0	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	34.0
16	26.0
17	13.0
18	51.0
19	280.0
20	1113.0
21	2785.0
22	4518.0
23	6340.0
24	5608.0
25	4166.0
26	4144.0
27	5050.0
28	6352.0
29	8579.0
30	11332.0
31	15609.0
32	22087.0
33	33401.0
34	55699.0
35	116440.0
36	339526.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.112990167870926	20.74933825198991	12.393894810838049	24.743776769301114
2	17.312467445639076	21.105021340112415	35.552549540934926	26.02996167331359
3	19.830212001772512	25.193304879850114	30.006608049381565	24.969875068995808
4	15.471231662662966	17.59062745372422	34.68230830826162	32.2558325753512
5	16.000584632559992	34.70385095601418	32.77736228093771	16.51820213048812
6	30.385527369411253	34.87114103351499	18.48541153239161	16.25792006468215
7	26.006462507813787	31.80405974865261	23.088382102771913	19.10109564076169
8	25.648094160816598	32.45329663922383	21.575358972564935	20.32325022739464
9	26.29957008808141	16.043721964378726	21.253819063833756	36.402888883706105
10-11	25.084544161205308	25.321112329065308	27.456678405672037	22.13766510405734
12-13	26.09775248579269	24.099556094565074	27.24980758915036	22.552883830491872
14-15	24.311557867077145	24.584742402686754	25.61723068311682	25.486469047119282
16-17	23.407654453436574	26.66993182047873	26.513748629801526	23.408665096283165
18-19	23.27207282847836	25.665041863936377	28.49468635087965	22.568198956705615
20-21	24.21558444789273	24.81404142709211	28.371509778373454	22.598864346641705
22-23	24.10686381976351	24.72094596170441	28.22375632623551	22.948433892296567
24-25	23.527143534606743	25.21343999502453	28.274132985050258	22.98528348531847
26-27	23.240898383748863	25.269414060374253	28.272111699357076	23.21757585651981
28-29	23.126618000326516	25.175268792126314	28.600959333286692	23.09715387426048
30-31	23.597033374536462	24.976949568921956	28.561855229299315	22.864161827242267
32-33	23.144420862778023	25.155289160466758	28.3747308191649	23.325559157590316
34-35	23.322138520263387	25.142228545218494	28.45379418647138	23.08183874804674
36-37	23.339337593066674	25.201720581913058	28.19143767166365	23.26750415335662
38-39	23.292596652439926	25.184831028290226	28.187839634302776	23.334732684967076
40-41	23.119030256289854	25.225884295618833	28.371394053229547	23.283691394861762
42-43	23.384290543166955	25.157427303569595	28.077256768985343	23.381025384278104
44-45	23.355800701230653	25.22409061579246	27.913178005301987	23.5069306776749
46-47	23.361684924158197	25.236233022087806	27.84610839429962	23.555973659454374
48-49	23.48933437413504	25.34613913362588	27.746488071728237	23.418038420510843
50-51	23.228926153104617	25.61412101281962	27.490807037183885	23.666145796891886
52-53	23.260178339591544	25.47270875605414	27.358646049552597	23.90846685480172
54-55	23.241831284838025	25.43648109709168	27.43506619710645	23.88662142096384
56-57	23.411152832520933	25.33837099921481	27.05016675606969	24.200309412194574
58-59	23.21166748295512	25.379885097682518	27.42993524111606	23.9785121782463
60-61	23.320661426872217	25.468199734123186	27.60944095902232	23.601697879982275
62-63	22.93545101880573	25.535601837815147	27.643569590534163	23.88537755284496
64-65	23.0008318368045	25.254254417675366	27.709183633805225	24.035730111714905
66-67	23.20688982239752	25.34335986913256	27.479985414251583	23.969764894218336
68-69	23.18445786785456	25.221680621312125	27.607575156843993	23.98628635398932
70-71	23.046621731930873	25.239328000248772	27.865600049754725	23.84845021806563
72-73	23.213533285133444	25.32173426312475	27.799830522968804	23.664901928773002
74-75	23.155978434225162	25.274045605545485	27.92145452282222	23.648521437407126
76-77	23.018591454650664	25.193505434951685	28.00454520000747	23.783357910390183
78-79	23.04315309600219	25.270387093564196	27.926524367490114	23.7599354429435
80-81	22.858486678949863	25.535290870785428	27.844454291733715	23.76176815853099
82-83	23.047787858292324	25.323055873001067	27.94264213136802	23.686514137338587
84-85	22.932281404048663	25.441166975622924	27.711781540570247	23.914770079758167
86-87	22.753411476897785	25.515003486465115	27.79012648661704	23.941458550020062
88-89	23.030122134453887	25.161552273421574	27.691914437302444	24.1164111548221
90-91	23.15777426384545	25.138215104043798	27.72430320201244	23.97970743009831
92-93	22.970216270025038	25.356526043543536	27.570750660204585	24.102507026226835
94-95	22.492340487394554	25.486022409856417	27.829394025852267	24.192243076896762
96-97	22.706579284931315	25.40911599847626	27.491739938273046	24.39256477831938
98-99	22.763253026097907	25.408027613872243	27.34154286291796	24.487176497111893
100-101	22.816272904665283	25.608290381012356	27.17058873832902	24.404847975993345
102-103	22.917337189324503	25.56545467266833	27.274140759226	24.243067378781163
104-105	22.836507813107364	25.74531602270077	27.139703024177877	24.278473140013993
106-107	22.78042105917841	25.642550611064465	27.235827347078395	24.341200982678732
108-109	22.89479781009527	25.656745960788825	27.10605737848936	24.34239885062654
110-111	22.711893922432022	25.91012699952733	26.97612694977237	24.401852128268278
112-113	22.714664427704054	25.944134773110683	26.903157092769238	24.43804370641603
114-115	22.65806842829489	26.02677426125895	26.99870171265092	24.316455597795244
116-117	22.668425704865626	25.966855553370145	26.974000803850394	24.39071793791383
118-119	22.499397501379917	25.73120009950945	27.18100613382466	24.588396265285972
120-121	22.546260520468756	25.800239757071047	27.196179435311457	24.45732028714874
122-123	22.33909399755891	26.06743320039493	27.18629257332991	24.40718022871625
124-125	22.32105790983511	25.975075992567888	27.07994184916544	24.62392424843156
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	192.0
1	172.0
2	257.0
3	336.0
4	270.0
5	285.0
6	466.5
7	813.5
8	1148.0
9	1356.5
10	1265.5
11	1004.0
12	953.0
13	938.5
14	890.0
15	915.5
16	934.0
17	904.0
18	880.5
19	867.0
20	867.5
21	879.5
22	930.0
23	1055.5
24	1171.5
25	1308.5
26	1621.0
27	2071.5
28	2603.0
29	3517.5
30	4885.0
31	6083.5
32	7309.0
33	8824.5
34	10164.0
35	11405.5
36	12703.0
37	14133.0
38	15047.5
39	15435.5
40	15696.0
41	15880.0
42	16409.5
43	17645.0
44	19433.5
45	20941.0
46	22428.5
47	23806.5
48	25354.5
49	26883.0
50	27817.5
51	27118.5
52	25313.5
53	24944.0
54	24703.0
55	23496.5
56	22045.5
57	19968.0
58	18186.5
59	16363.5
60	13987.5
61	11718.5
62	9504.0
63	7556.0
64	5751.0
65	4386.0
66	3411.0
67	2645.0
68	2174.5
69	1804.5
70	1542.5
71	1385.5
72	1221.0
73	1146.5
74	923.5
75	746.0
76	625.5
77	436.5
78	312.5
79	217.5
80	162.5
81	119.5
82	70.5
83	41.0
84	31.5
85	16.0
86	6.5
87	3.0
88	1.5
89	2.0
90	1.0
91	1.0
92	0.5
93	0.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.025032845892514248
2	0.0
3	0.0
4	0.0
5	0.0024877362377653906
6	0.0015548351486033693
7	0.009484494406480552
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	1.5548351486033692E-4
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	7.774175743016846E-5
38-39	0.0
40-41	0.0024877362377653906
42-43	1.5548351486033692E-4
44-45	0.0
46-47	0.006219340594413477
48-49	0.009484494406480552
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.009562236163910722
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.07307725198435837
76-77	0.043379900646034
78-79	4.6645054458101086E-4
80-81	0.0
82-83	0.0
84-85	0.062115664186704604
86-87	0.21573337686871752
88-89	0.243642667786148
90-91	0.14763159735988993
92-93	0.1151355427540795
94-95	7.774175743016846E-5
96-97	0.0
98-99	0.0
100-101	0.0
102-103	0.0
104-105	7.774175743016846E-4
106-107	0.002332252722905054
108-109	0.0034206373269274125
110-111	4.6645054458101086E-4
112-113	0.0
114-115	0.0
116-117	7.774175743016846E-5
118-119	0.0
120-121	6.219340594413477E-4
122-123	0.0
124-125	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	643155.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.38582797494541
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.54466509366364	66.10785899993854
2	9.473119486582961	14.472241509764583
3	1.987181024887293	4.553774037663494
4	0.7462294955572762	2.280054315098736
5	0.3626884340610506	1.385212816634487
6	0.20815687254763082	0.9540141034935579
7	0.14072057346246092	0.7524340261927445
8	0.09803279751867686	0.5990653125731445
9	0.06556662117210228	0.4507524581175556
>10	0.34161536253326946	4.648539688616857
>50	0.020136460852035687	1.054830381468834
>100	0.010432130978533665	1.4326926711230565
>500	7.278230915231116E-4	0.32004401022178
>1k	7.278230915231116E-4	0.9884856690926311
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2144	0.3333566558605624	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2036	0.316564436255646	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1154	0.17942797614882883	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	658	0.1023081527781017	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	7.774175743016847E-5	0.0
14-15	0.0	0.0	0.0	1.5548351486033694E-4	0.0
16-17	0.0	0.0	0.0	2.332252722905054E-4	0.0
18-19	0.0	0.0	0.0	4.664505445810108E-4	0.0
20-21	0.0	0.0	0.0	0.00101064284659219	0.0
22-23	0.0	0.0	0.0	0.002876445024916233	0.0
24-25	0.0	0.0	0.0	0.006219340594413477	0.0
26-27	0.0	0.0	0.0	0.010883846040223584	0.0
28-29	1.5548351486033694E-4	0.0	0.0	0.022000917352737674	0.0
30-31	1.5548351486033694E-4	0.0	0.0	0.041436356710279794	0.0
32-33	1.5548351486033694E-4	0.0	0.0	0.079374334336202	0.0
34-35	1.5548351486033694E-4	0.0	0.0	0.12345391079910753	0.0
36-37	1.5548351486033694E-4	0.0	0.0	0.18176022887173388	0.0
38-39	1.5548351486033694E-4	0.0	0.0	0.2576361841235783	0.0
40-41	1.5548351486033694E-4	0.0	0.0	0.34828307328715474	0.0
42-43	1.5548351486033694E-4	0.0	0.0	0.42827934168279813	0.0
44-45	1.5548351486033694E-4	0.0	0.0	0.5088198023804525	0.0
46-47	1.5548351486033694E-4	0.0	0.0	0.605452806866152	0.0
48-49	1.5548351486033694E-4	0.0	0.0	0.7096267618225778	0.0
50-51	1.5548351486033694E-4	0.0	0.0	0.8085920190311822	0.0
52-53	1.5548351486033694E-4	0.0	0.0	0.905846957576323	0.0
54-55	1.5548351486033694E-4	0.0	0.0	1.0002254510965474	0.0
56-57	1.5548351486033694E-4	0.0	0.0	1.102067153330068	0.0
58-59	1.5548351486033694E-4	0.0	0.0	1.2223336520745387	0.0
60-61	1.5548351486033694E-4	0.0	0.0	1.3324937223530875	0.0
62-63	1.5548351486033694E-4	0.0	0.0	1.439310897062139	0.0
64-65	1.5548351486033694E-4	0.0	0.0	1.5413858245679504	0.0
66-67	1.5548351486033694E-4	0.0	0.0	1.657143301381471	0.0
68-69	1.5548351486033694E-4	0.0	0.0	1.7899262230721988	0.0
70-71	1.5548351486033694E-4	0.0	0.0	1.9311829963228149	0.0
72-73	1.5548351486033694E-4	0.0	0.0	2.071506868484269	3.109670297206739E-4
74-75	1.5548351486033694E-4	1.5548351486033694E-4	0.0	2.1996252847291866	3.109670297206739E-4
76-77	1.5548351486033694E-4	1.5548351486033694E-4	0.0	2.3371504536231544	3.109670297206739E-4
78-79	1.5548351486033694E-4	1.5548351486033694E-4	0.0	2.4920897761814804	3.109670297206739E-4
80-81	1.5548351486033694E-4	1.5548351486033694E-4	0.0	2.6530929558193597	3.109670297206739E-4
82-83	1.5548351486033694E-4	1.5548351486033694E-4	0.0	2.8225699870171264	4.664505445810108E-4
84-85	1.5548351486033694E-4	1.5548351486033694E-4	0.0	2.9790641447240556	4.664505445810108E-4
86-87	1.5548351486033694E-4	1.5548351486033694E-4	0.0	3.1528169725804824	6.219340594413478E-4
88-89	1.5548351486033694E-4	1.5548351486033694E-4	0.0	3.3411852508337803	6.219340594413478E-4
90-91	1.5548351486033694E-4	1.5548351486033694E-4	0.0	3.532585457626855	6.219340594413478E-4
92-93	1.5548351486033694E-4	1.5548351486033694E-4	0.0	3.7265511424151256	6.219340594413478E-4
94-95	1.5548351486033694E-4	1.5548351486033694E-4	0.0	3.9143752283664126	6.219340594413478E-4
96-97	1.5548351486033694E-4	1.5548351486033694E-4	0.0	4.106397369218929	6.219340594413478E-4
98-99	1.5548351486033694E-4	1.5548351486033694E-4	0.0	4.324618482325412	6.219340594413478E-4
100-101	1.5548351486033694E-4	1.5548351486033694E-4	0.0	4.55939858976452	6.219340594413478E-4
102-103	1.5548351486033694E-4	1.5548351486033694E-4	0.0	4.805451252031004	6.219340594413478E-4
104-105	1.5548351486033694E-4	1.5548351486033694E-4	0.0	5.065497430634917	6.219340594413478E-4
106-107	1.5548351486033694E-4	1.5548351486033694E-4	0.0	5.316681048891791	6.219340594413478E-4
108-109	1.5548351486033694E-4	1.5548351486033694E-4	0.0	5.559935007890788	6.219340594413478E-4
110-111	1.5548351486033694E-4	1.5548351486033694E-4	0.0	5.83265309295582	6.219340594413478E-4
112-113	1.5548351486033694E-4	1.5548351486033694E-4	0.0	6.131025957972806	6.219340594413478E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2750	0.0	30.29079	1
GTATCAA	3670	0.0	27.88545	1
CGAATTA	55	7.7695487E-4	27.034834	66-67
TATCAAC	4440	0.0	23.174454	2
CAACGCA	4555	0.0	22.981094	5
ATCAACG	4470	0.0	22.353634	3
TCAACGC	4640	0.0	22.30374	4
AACGCAG	4745	0.0	22.059166	6
ACGCAGA	5250	0.0	19.710722	7
CGCAGAG	5410	0.0	19.456059	8
CATGGGG	1980	0.0	18.32361	4
GAGTACT	2285	0.0	18.21758	12-13
GCAGAGT	5870	0.0	17.830086	9
CAGAGTA	5780	0.0	16.975985	10-11
GTGTTAA	285	3.8880236E-5	16.701689	1
ATTATAC	330	7.523E-6	16.2209	3
GTACTTT	2665	0.0	16.06686	14-15
GCTTAGA	260	3.6597202E-4	16.019167	1
ACATGGG	4615	0.0	15.59409	3
AGTACTT	2445	0.0	15.566105	12-13
>>END_MODULE