FastQCFastQC Report
Sun 1 Jan 2017
ERR1142508_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142508_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71707
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3300.4602061165576582No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT2230.3109877696738115No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG2000.27891279791373225No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG1810.2524160821119277No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1650.2301030582788291No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT1420.1980280865187499No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA1280.17850419066478865No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1240.172925934706514No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG1200.16734767874823936No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC1050.14642921890470945No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT910.12690532305074817No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA860.11993250310290487No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT850.11853793911333621No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT810.11295968315506158No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC800.11156511916549292No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC790.11017055517592424No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC780.10877599118635559No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC780.10877599118635559No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC760.10598686320721826No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT720.10040860724894363No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCAC250.00204459471.438215
TACAAGA359.069878E-568.0363852
GAACAAA807.2759576E-1267.020221
GTACAAA753.1286618E-1063.5450971
GTACAAG507.327071E-659.573531
AACTCGC150.00423173759.4902128-29
CCTATAG150.00424347359.44864116-117
TCTCTGC350.00775013151.027296
TTCTCTG350.00775013151.027295
TAATGTG600.001296881839.6878976
TACAAAA957.3474675E-637.5990562
ATTTAGG700.002764342834.0181923
CCATAAT700.002764342834.0181923
AATGTGA700.002764342834.0181927
CAGGATT350.002783112733.99440464-65
AGCATTG504.4055964E-429.7659224-25
GTACTAG850.007122799828.0346011
ACCATAA850.007147216228.0149842
CATAATG850.007147216228.0149844
TAGGCAT1107.655946E-427.0599255
Produced by FastQC (version 0.11.5)